Experiment: nitrate 100 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2298 and Pf1N1B4_2299 overlap by 8 nucleotides Pf1N1B4_2299 and Pf1N1B4_2300 are separated by 85 nucleotides Pf1N1B4_2300 and Pf1N1B4_2301 are separated by 110 nucleotides
Pf1N1B4_2298 - probable integral membrane protein NMA1898, at 2,615,717 to 2,617,093
_2298
Pf1N1B4_2299 - probable integral membrane protein NMA1899, at 2,617,086 to 2,618,465
_2299
Pf1N1B4_2300 - Dihydrofolate reductase (EC 1.5.1.3), at 2,618,551 to 2,619,063
_2300
Pf1N1B4_2301 - L-cystine uptake protein TcyP, at 2,619,174 to 2,620,565
_2301
Position (kb)
2617
2618
2619 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2616.255 kb on + strand, within Pf1N1B4_2298 at 2616.255 kb on + strand, within Pf1N1B4_2298 at 2616.255 kb on + strand, within Pf1N1B4_2298 at 2616.255 kb on + strand, within Pf1N1B4_2298 at 2616.255 kb on + strand, within Pf1N1B4_2298 at 2616.256 kb on - strand, within Pf1N1B4_2298 at 2616.386 kb on + strand, within Pf1N1B4_2298 at 2616.387 kb on - strand, within Pf1N1B4_2298 at 2616.387 kb on - strand, within Pf1N1B4_2298 at 2616.387 kb on - strand, within Pf1N1B4_2298 at 2616.504 kb on + strand, within Pf1N1B4_2298 at 2616.504 kb on + strand, within Pf1N1B4_2298 at 2616.504 kb on + strand, within Pf1N1B4_2298 at 2616.720 kb on + strand, within Pf1N1B4_2298 at 2616.720 kb on + strand at 2616.720 kb on + strand, within Pf1N1B4_2298 at 2616.721 kb on - strand, within Pf1N1B4_2298 at 2616.865 kb on - strand, within Pf1N1B4_2298 at 2616.865 kb on - strand, within Pf1N1B4_2298 at 2617.045 kb on + strand at 2617.153 kb on - strand at 2617.377 kb on + strand, within Pf1N1B4_2299 at 2617.378 kb on - strand, within Pf1N1B4_2299 at 2617.449 kb on + strand, within Pf1N1B4_2299 at 2617.450 kb on - strand, within Pf1N1B4_2299 at 2618.056 kb on - strand, within Pf1N1B4_2299 at 2618.056 kb on - strand, within Pf1N1B4_2299 at 2618.119 kb on - strand, within Pf1N1B4_2299 at 2618.119 kb on - strand, within Pf1N1B4_2299 at 2618.418 kb on + strand at 2618.418 kb on + strand at 2618.418 kb on + strand at 2618.418 kb on + strand at 2618.419 kb on - strand at 2618.419 kb on - strand at 2618.482 kb on + strand at 2618.483 kb on - strand at 2618.483 kb on - strand at 2618.483 kb on - strand at 2618.483 kb on - strand at 2618.500 kb on - strand at 2619.062 kb on - strand at 2619.062 kb on - strand at 2619.062 kb on - strand at 2619.062 kb on - strand at 2619.062 kb on - strand at 2619.062 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate 100 mM remove 2,616,255 + Pf1N1B4_2298 0.39 -1.0 2,616,255 + Pf1N1B4_2298 0.39 -0.1 2,616,255 + Pf1N1B4_2298 0.39 -0.1 2,616,255 + Pf1N1B4_2298 0.39 +0.9 2,616,255 + Pf1N1B4_2298 0.39 -2.6 2,616,256 - Pf1N1B4_2298 0.39 -2.3 2,616,386 + Pf1N1B4_2298 0.49 +0.4 2,616,387 - Pf1N1B4_2298 0.49 -0.3 2,616,387 - Pf1N1B4_2298 0.49 -0.6 2,616,387 - Pf1N1B4_2298 0.49 +2.3 2,616,504 + Pf1N1B4_2298 0.57 -0.3 2,616,504 + Pf1N1B4_2298 0.57 -0.5 2,616,504 + Pf1N1B4_2298 0.57 +0.7 2,616,720 + Pf1N1B4_2298 0.73 +0.8 2,616,720 + +1.7 2,616,720 + Pf1N1B4_2298 0.73 -0.8 2,616,721 - Pf1N1B4_2298 0.73 +1.3 2,616,865 - Pf1N1B4_2298 0.83 -0.3 2,616,865 - Pf1N1B4_2298 0.83 +1.0 2,617,045 + +1.7 2,617,153 - -2.4 2,617,377 + Pf1N1B4_2299 0.21 -0.5 2,617,378 - Pf1N1B4_2299 0.21 -0.7 2,617,449 + Pf1N1B4_2299 0.26 -1.3 2,617,450 - Pf1N1B4_2299 0.26 -1.0 2,618,056 - Pf1N1B4_2299 0.70 -1.5 2,618,056 - Pf1N1B4_2299 0.70 +0.3 2,618,119 - Pf1N1B4_2299 0.75 -1.5 2,618,119 - Pf1N1B4_2299 0.75 -1.1 2,618,418 + +0.9 2,618,418 + -1.3 2,618,418 + -1.0 2,618,418 + -0.3 2,618,419 - -0.9 2,618,419 - -0.3 2,618,482 + -0.3 2,618,483 - -1.0 2,618,483 - -0.7 2,618,483 - -1.3 2,618,483 - +1.3 2,618,500 - -1.4 2,619,062 - +0.3 2,619,062 - +0.9 2,619,062 - -0.5 2,619,062 - -0.2 2,619,062 - +0.3 2,619,062 - +0.5
Or see this region's nucleotide sequence