Experiment: nitrate 100 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_223 and Pf1N1B4_224 overlap by 4 nucleotides Pf1N1B4_224 and Pf1N1B4_225 are separated by 116 nucleotides
Pf1N1B4_223 - Citronellyl-CoA dehydrogenase, at 283,755 to 284,912
_223
Pf1N1B4_224 - Isohexenylglutaconyl-CoA hydratase, at 284,909 to 285,706
_224
Pf1N1B4_225 - Methylcrotonyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.4), at 285,823 to 287,784
_225
Position (kb) 284
285
286 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 283.948 kb on - strand, within Pf1N1B4_223 at 283.948 kb on - strand, within Pf1N1B4_223 at 283.948 kb on - strand, within Pf1N1B4_223 at 283.954 kb on - strand, within Pf1N1B4_223 at 284.156 kb on - strand, within Pf1N1B4_223 at 284.196 kb on + strand, within Pf1N1B4_223 at 284.196 kb on + strand, within Pf1N1B4_223 at 284.197 kb on - strand at 284.197 kb on - strand, within Pf1N1B4_223 at 284.197 kb on - strand, within Pf1N1B4_223 at 284.332 kb on + strand, within Pf1N1B4_223 at 284.333 kb on - strand, within Pf1N1B4_223 at 284.520 kb on + strand, within Pf1N1B4_223 at 284.520 kb on + strand, within Pf1N1B4_223 at 284.520 kb on + strand, within Pf1N1B4_223 at 284.520 kb on + strand, within Pf1N1B4_223 at 284.521 kb on - strand, within Pf1N1B4_223 at 284.544 kb on + strand, within Pf1N1B4_223 at 284.653 kb on - strand, within Pf1N1B4_223 at 284.670 kb on + strand at 284.670 kb on + strand, within Pf1N1B4_223 at 284.670 kb on + strand, within Pf1N1B4_223 at 284.671 kb on - strand, within Pf1N1B4_223 at 284.671 kb on - strand, within Pf1N1B4_223 at 285.288 kb on + strand, within Pf1N1B4_224 at 285.289 kb on - strand, within Pf1N1B4_224 at 285.289 kb on - strand, within Pf1N1B4_224 at 285.289 kb on - strand, within Pf1N1B4_224 at 285.806 kb on + strand at 285.806 kb on + strand at 285.806 kb on + strand at 285.807 kb on - strand at 285.807 kb on - strand at 285.807 kb on - strand at 285.807 kb on - strand at 285.807 kb on - strand at 285.807 kb on - strand at 285.920 kb on + strand at 286.006 kb on + strand at 286.007 kb on - strand at 286.007 kb on - strand at 286.495 kb on + strand, within Pf1N1B4_225 at 286.495 kb on + strand, within Pf1N1B4_225 at 286.631 kb on - strand, within Pf1N1B4_225 at 286.631 kb on - strand, within Pf1N1B4_225 at 286.631 kb on - strand, within Pf1N1B4_225 at 286.631 kb on - strand, within Pf1N1B4_225 at 286.682 kb on - strand, within Pf1N1B4_225 at 286.682 kb on - strand, within Pf1N1B4_225
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate 100 mM remove 283,948 - Pf1N1B4_223 0.17 -0.6 283,948 - Pf1N1B4_223 0.17 -0.4 283,948 - Pf1N1B4_223 0.17 -0.5 283,954 - Pf1N1B4_223 0.17 -0.6 284,156 - Pf1N1B4_223 0.35 -0.5 284,196 + Pf1N1B4_223 0.38 -0.8 284,196 + Pf1N1B4_223 0.38 +0.6 284,197 - -0.2 284,197 - Pf1N1B4_223 0.38 -0.6 284,197 - Pf1N1B4_223 0.38 +1.2 284,332 + Pf1N1B4_223 0.50 -1.2 284,333 - Pf1N1B4_223 0.50 +0.1 284,520 + Pf1N1B4_223 0.66 -1.2 284,520 + Pf1N1B4_223 0.66 -1.5 284,520 + Pf1N1B4_223 0.66 +2.1 284,520 + Pf1N1B4_223 0.66 -1.8 284,521 - Pf1N1B4_223 0.66 -0.9 284,544 + Pf1N1B4_223 0.68 +0.7 284,653 - Pf1N1B4_223 0.78 -0.8 284,670 + -0.5 284,670 + Pf1N1B4_223 0.79 -1.0 284,670 + Pf1N1B4_223 0.79 +0.1 284,671 - Pf1N1B4_223 0.79 -0.8 284,671 - Pf1N1B4_223 0.79 +0.2 285,288 + Pf1N1B4_224 0.47 -2.3 285,289 - Pf1N1B4_224 0.48 +0.0 285,289 - Pf1N1B4_224 0.48 -0.5 285,289 - Pf1N1B4_224 0.48 -3.2 285,806 + +0.8 285,806 + +0.3 285,806 + +0.3 285,807 - +0.8 285,807 - -0.2 285,807 - +1.8 285,807 - -2.3 285,807 - +1.1 285,807 - -1.2 285,920 + -1.5 286,006 + -1.0 286,007 - -0.8 286,007 - -0.9 286,495 + Pf1N1B4_225 0.34 +0.5 286,495 + Pf1N1B4_225 0.34 -0.5 286,631 - Pf1N1B4_225 0.41 +0.5 286,631 - Pf1N1B4_225 0.41 -0.0 286,631 - Pf1N1B4_225 0.41 +0.4 286,631 - Pf1N1B4_225 0.41 +1.8 286,682 - Pf1N1B4_225 0.44 +1.8 286,682 - Pf1N1B4_225 0.44 -0.7
Or see this region's nucleotide sequence