Experiment: nitrate 100 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2205 and Pf1N1B4_2206 overlap by 20 nucleotides Pf1N1B4_2206 and Pf1N1B4_2207 are separated by 198 nucleotides Pf1N1B4_2207 and Pf1N1B4_2208 are separated by 18 nucleotides
Pf1N1B4_2205 - Sterol desaturase, at 2,517,391 to 2,518,638
_2205
Pf1N1B4_2206 - FIG139438: lipoprotein B, at 2,518,619 to 2,519,251
_2206
Pf1N1B4_2207 - Porphobilinogen synthase (EC 4.2.1.24), at 2,519,450 to 2,520,463
_2207
Pf1N1B4_2208 - Polyphosphate kinase (EC 2.7.4.1), at 2,520,482 to 2,522,704
_2208
Position (kb)
2519
2520
2521 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2518.466 kb on + strand, within Pf1N1B4_2205 at 2518.466 kb on + strand, within Pf1N1B4_2205 at 2518.466 kb on + strand, within Pf1N1B4_2205 at 2518.467 kb on - strand, within Pf1N1B4_2205 at 2518.535 kb on + strand at 2518.535 kb on + strand at 2518.535 kb on + strand at 2518.535 kb on + strand at 2518.535 kb on + strand at 2518.535 kb on + strand at 2518.535 kb on + strand at 2518.536 kb on - strand at 2518.536 kb on - strand at 2518.536 kb on - strand at 2518.536 kb on - strand at 2518.573 kb on + strand at 2518.574 kb on - strand at 2518.600 kb on + strand at 2518.600 kb on + strand at 2518.601 kb on - strand at 2518.608 kb on + strand at 2518.609 kb on - strand at 2518.636 kb on + strand at 2518.636 kb on + strand at 2518.637 kb on - strand at 2518.637 kb on - strand at 2518.637 kb on - strand at 2518.686 kb on + strand, within Pf1N1B4_2206 at 2518.686 kb on + strand, within Pf1N1B4_2206 at 2518.686 kb on + strand, within Pf1N1B4_2206 at 2518.686 kb on + strand, within Pf1N1B4_2206 at 2518.687 kb on - strand, within Pf1N1B4_2206 at 2518.687 kb on - strand, within Pf1N1B4_2206 at 2518.687 kb on - strand, within Pf1N1B4_2206 at 2518.749 kb on - strand, within Pf1N1B4_2206 at 2518.779 kb on + strand, within Pf1N1B4_2206 at 2518.779 kb on + strand, within Pf1N1B4_2206 at 2518.779 kb on + strand, within Pf1N1B4_2206 at 2518.779 kb on + strand, within Pf1N1B4_2206 at 2518.780 kb on - strand, within Pf1N1B4_2206 at 2518.780 kb on - strand, within Pf1N1B4_2206 at 2518.780 kb on - strand, within Pf1N1B4_2206 at 2518.828 kb on - strand, within Pf1N1B4_2206 at 2518.888 kb on - strand, within Pf1N1B4_2206 at 2518.888 kb on - strand, within Pf1N1B4_2206 at 2518.993 kb on + strand, within Pf1N1B4_2206 at 2518.994 kb on - strand at 2518.994 kb on - strand, within Pf1N1B4_2206 at 2519.076 kb on + strand, within Pf1N1B4_2206 at 2519.076 kb on + strand, within Pf1N1B4_2206 at 2519.077 kb on - strand, within Pf1N1B4_2206 at 2519.097 kb on + strand, within Pf1N1B4_2206 at 2519.097 kb on + strand at 2519.097 kb on + strand, within Pf1N1B4_2206 at 2519.097 kb on + strand, within Pf1N1B4_2206 at 2519.097 kb on + strand at 2519.098 kb on - strand, within Pf1N1B4_2206 at 2519.098 kb on - strand, within Pf1N1B4_2206 at 2519.098 kb on - strand, within Pf1N1B4_2206 at 2519.098 kb on - strand at 2519.126 kb on + strand, within Pf1N1B4_2206 at 2519.126 kb on + strand, within Pf1N1B4_2206 at 2519.127 kb on - strand at 2519.127 kb on - strand, within Pf1N1B4_2206 at 2519.142 kb on + strand, within Pf1N1B4_2206 at 2519.142 kb on + strand, within Pf1N1B4_2206 at 2519.142 kb on + strand, within Pf1N1B4_2206 at 2519.143 kb on - strand, within Pf1N1B4_2206 at 2519.143 kb on - strand, within Pf1N1B4_2206 at 2519.143 kb on - strand, within Pf1N1B4_2206 at 2519.285 kb on - strand at 2519.341 kb on + strand at 2519.342 kb on - strand at 2519.342 kb on - strand at 2519.394 kb on + strand at 2519.395 kb on - strand at 2519.405 kb on + strand at 2519.405 kb on + strand at 2519.405 kb on + strand at 2519.405 kb on + strand at 2519.405 kb on + strand at 2519.405 kb on + strand at 2519.405 kb on + strand at 2519.405 kb on + strand at 2519.405 kb on + strand at 2519.405 kb on + strand at 2519.405 kb on + strand at 2519.405 kb on + strand at 2519.405 kb on + strand at 2519.406 kb on - strand at 2519.406 kb on - strand at 2519.406 kb on - strand at 2519.406 kb on - strand at 2519.406 kb on - strand at 2519.406 kb on - strand at 2519.407 kb on + strand at 2519.407 kb on + strand at 2519.407 kb on + strand at 2519.407 kb on + strand at 2519.407 kb on + strand at 2519.407 kb on + strand at 2519.407 kb on + strand at 2519.407 kb on + strand at 2519.407 kb on + strand at 2519.407 kb on + strand at 2519.407 kb on + strand at 2519.407 kb on + strand at 2519.407 kb on + strand at 2519.407 kb on + strand at 2519.407 kb on + strand at 2519.408 kb on - strand at 2519.408 kb on - strand at 2519.408 kb on - strand at 2519.408 kb on - strand at 2519.544 kb on + strand at 2519.544 kb on + strand at 2519.544 kb on + strand at 2519.544 kb on + strand at 2519.545 kb on - strand at 2519.822 kb on - strand, within Pf1N1B4_2207 at 2519.848 kb on + strand, within Pf1N1B4_2207 at 2519.888 kb on + strand, within Pf1N1B4_2207 at 2519.888 kb on + strand, within Pf1N1B4_2207 at 2519.888 kb on + strand, within Pf1N1B4_2207 at 2519.888 kb on + strand, within Pf1N1B4_2207 at 2519.906 kb on - strand, within Pf1N1B4_2207 at 2519.924 kb on - strand, within Pf1N1B4_2207 at 2520.053 kb on + strand, within Pf1N1B4_2207 at 2520.054 kb on - strand, within Pf1N1B4_2207 at 2520.054 kb on - strand, within Pf1N1B4_2207 at 2520.065 kb on + strand, within Pf1N1B4_2207 at 2520.065 kb on + strand, within Pf1N1B4_2207 at 2520.065 kb on + strand, within Pf1N1B4_2207 at 2520.077 kb on + strand, within Pf1N1B4_2207 at 2520.077 kb on + strand, within Pf1N1B4_2207 at 2520.077 kb on + strand, within Pf1N1B4_2207 at 2520.078 kb on - strand, within Pf1N1B4_2207 at 2520.080 kb on + strand, within Pf1N1B4_2207 at 2520.143 kb on + strand, within Pf1N1B4_2207 at 2520.143 kb on + strand, within Pf1N1B4_2207 at 2520.143 kb on + strand, within Pf1N1B4_2207 at 2520.144 kb on - strand, within Pf1N1B4_2207 at 2520.242 kb on + strand, within Pf1N1B4_2207 at 2520.242 kb on + strand, within Pf1N1B4_2207 at 2520.277 kb on + strand, within Pf1N1B4_2207 at 2520.277 kb on + strand, within Pf1N1B4_2207 at 2520.277 kb on + strand, within Pf1N1B4_2207 at 2520.278 kb on - strand, within Pf1N1B4_2207 at 2520.296 kb on + strand, within Pf1N1B4_2207 at 2520.296 kb on + strand, within Pf1N1B4_2207 at 2520.296 kb on + strand, within Pf1N1B4_2207 at 2520.296 kb on + strand, within Pf1N1B4_2207 at 2520.296 kb on + strand, within Pf1N1B4_2207 at 2520.296 kb on + strand, within Pf1N1B4_2207 at 2520.296 kb on + strand, within Pf1N1B4_2207 at 2520.314 kb on + strand, within Pf1N1B4_2207 at 2520.314 kb on + strand, within Pf1N1B4_2207 at 2520.314 kb on + strand, within Pf1N1B4_2207 at 2520.314 kb on + strand, within Pf1N1B4_2207 at 2520.314 kb on + strand, within Pf1N1B4_2207 at 2520.388 kb on + strand at 2520.419 kb on + strand at 2520.419 kb on + strand at 2520.419 kb on + strand at 2520.419 kb on + strand at 2520.419 kb on + strand at 2520.419 kb on + strand at 2520.419 kb on + strand at 2520.419 kb on + strand at 2520.420 kb on - strand at 2520.420 kb on - strand at 2520.420 kb on - strand at 2520.461 kb on + strand at 2520.461 kb on + strand at 2520.650 kb on + strand at 2520.650 kb on + strand at 2520.651 kb on - strand at 2520.651 kb on - strand at 2520.651 kb on - strand at 2520.651 kb on - strand at 2521.232 kb on + strand, within Pf1N1B4_2208 at 2521.232 kb on + strand, within Pf1N1B4_2208 at 2521.232 kb on + strand, within Pf1N1B4_2208 at 2521.232 kb on + strand, within Pf1N1B4_2208 at 2521.232 kb on + strand, within Pf1N1B4_2208 at 2521.233 kb on - strand, within Pf1N1B4_2208 at 2521.233 kb on - strand, within Pf1N1B4_2208 at 2521.460 kb on + strand, within Pf1N1B4_2208
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate 100 mM remove 2,518,466 + Pf1N1B4_2205 0.86 -0.4 2,518,466 + Pf1N1B4_2205 0.86 +1.3 2,518,466 + Pf1N1B4_2205 0.86 -1.3 2,518,467 - Pf1N1B4_2205 0.86 -1.1 2,518,535 + -0.7 2,518,535 + -0.0 2,518,535 + -1.6 2,518,535 + -1.3 2,518,535 + +0.5 2,518,535 + +0.3 2,518,535 + -0.3 2,518,536 - +0.3 2,518,536 - -1.1 2,518,536 - +0.5 2,518,536 - +0.3 2,518,573 + -0.3 2,518,574 - -0.3 2,518,600 + -2.9 2,518,600 + +0.0 2,518,601 - -0.3 2,518,608 + -0.7 2,518,609 - -0.2 2,518,636 + -1.5 2,518,636 + -1.1 2,518,637 - -1.3 2,518,637 - -0.4 2,518,637 - +0.7 2,518,686 + Pf1N1B4_2206 0.11 -1.5 2,518,686 + Pf1N1B4_2206 0.11 +0.7 2,518,686 + Pf1N1B4_2206 0.11 -0.8 2,518,686 + Pf1N1B4_2206 0.11 -0.6 2,518,687 - Pf1N1B4_2206 0.11 +0.6 2,518,687 - Pf1N1B4_2206 0.11 -0.4 2,518,687 - Pf1N1B4_2206 0.11 -1.5 2,518,749 - Pf1N1B4_2206 0.21 +0.3 2,518,779 + Pf1N1B4_2206 0.25 -0.3 2,518,779 + Pf1N1B4_2206 0.25 -1.5 2,518,779 + Pf1N1B4_2206 0.25 +0.7 2,518,779 + Pf1N1B4_2206 0.25 -0.7 2,518,780 - Pf1N1B4_2206 0.25 +0.3 2,518,780 - Pf1N1B4_2206 0.25 +0.7 2,518,780 - Pf1N1B4_2206 0.25 +0.6 2,518,828 - Pf1N1B4_2206 0.33 -1.5 2,518,888 - Pf1N1B4_2206 0.42 -0.7 2,518,888 - Pf1N1B4_2206 0.42 +1.3 2,518,993 + Pf1N1B4_2206 0.59 -0.3 2,518,994 - +1.3 2,518,994 - Pf1N1B4_2206 0.59 +0.0 2,519,076 + Pf1N1B4_2206 0.72 -1.0 2,519,076 + Pf1N1B4_2206 0.72 -2.0 2,519,077 - Pf1N1B4_2206 0.72 +0.7 2,519,097 + Pf1N1B4_2206 0.76 -1.8 2,519,097 + +0.3 2,519,097 + Pf1N1B4_2206 0.76 -0.3 2,519,097 + Pf1N1B4_2206 0.76 -0.3 2,519,097 + +2.3 2,519,098 - Pf1N1B4_2206 0.76 -0.9 2,519,098 - Pf1N1B4_2206 0.76 -0.7 2,519,098 - Pf1N1B4_2206 0.76 -1.5 2,519,098 - +1.3 2,519,126 + Pf1N1B4_2206 0.80 -0.3 2,519,126 + Pf1N1B4_2206 0.80 +0.6 2,519,127 - +2.1 2,519,127 - Pf1N1B4_2206 0.80 -0.5 2,519,142 + Pf1N1B4_2206 0.83 -0.7 2,519,142 + Pf1N1B4_2206 0.83 -0.8 2,519,142 + Pf1N1B4_2206 0.83 -1.3 2,519,143 - Pf1N1B4_2206 0.83 -1.0 2,519,143 - Pf1N1B4_2206 0.83 -1.3 2,519,143 - Pf1N1B4_2206 0.83 -0.3 2,519,285 - -0.7 2,519,341 + -1.3 2,519,342 - -0.3 2,519,342 - +0.6 2,519,394 + -2.0 2,519,395 - +0.8 2,519,405 + +0.3 2,519,405 + -0.8 2,519,405 + +0.7 2,519,405 + +0.4 2,519,405 + -0.4 2,519,405 + +0.1 2,519,405 + -0.3 2,519,405 + -0.9 2,519,405 + +0.3 2,519,405 + +2.3 2,519,405 + +0.7 2,519,405 + -0.4 2,519,405 + -0.7 2,519,406 - -1.8 2,519,406 - -1.0 2,519,406 - -1.0 2,519,406 - +0.3 2,519,406 - -1.3 2,519,406 - +2.3 2,519,407 + +1.3 2,519,407 + -1.3 2,519,407 + -2.3 2,519,407 + -0.9 2,519,407 + -1.0 2,519,407 + -3.6 2,519,407 + -0.8 2,519,407 + +1.9 2,519,407 + -0.3 2,519,407 + +0.7 2,519,407 + +0.1 2,519,407 + +1.9 2,519,407 + +0.1 2,519,407 + -0.3 2,519,407 + -1.7 2,519,408 - -0.7 2,519,408 - +0.3 2,519,408 - -1.3 2,519,408 - -0.8 2,519,544 + -1.0 2,519,544 + +0.7 2,519,544 + +1.0 2,519,544 + -2.0 2,519,545 - -0.7 2,519,822 - Pf1N1B4_2207 0.37 +0.3 2,519,848 + Pf1N1B4_2207 0.39 +0.1 2,519,888 + Pf1N1B4_2207 0.43 -0.3 2,519,888 + Pf1N1B4_2207 0.43 +0.3 2,519,888 + Pf1N1B4_2207 0.43 +0.1 2,519,888 + Pf1N1B4_2207 0.43 -2.8 2,519,906 - Pf1N1B4_2207 0.45 -0.0 2,519,924 - Pf1N1B4_2207 0.47 -0.3 2,520,053 + Pf1N1B4_2207 0.59 +0.7 2,520,054 - Pf1N1B4_2207 0.60 +0.9 2,520,054 - Pf1N1B4_2207 0.60 -0.7 2,520,065 + Pf1N1B4_2207 0.61 +0.5 2,520,065 + Pf1N1B4_2207 0.61 +0.5 2,520,065 + Pf1N1B4_2207 0.61 -0.1 2,520,077 + Pf1N1B4_2207 0.62 +0.9 2,520,077 + Pf1N1B4_2207 0.62 -0.6 2,520,077 + Pf1N1B4_2207 0.62 +0.3 2,520,078 - Pf1N1B4_2207 0.62 -0.3 2,520,080 + Pf1N1B4_2207 0.62 +0.7 2,520,143 + Pf1N1B4_2207 0.68 +1.9 2,520,143 + Pf1N1B4_2207 0.68 +0.6 2,520,143 + Pf1N1B4_2207 0.68 +2.1 2,520,144 - Pf1N1B4_2207 0.68 -0.6 2,520,242 + Pf1N1B4_2207 0.78 -0.5 2,520,242 + Pf1N1B4_2207 0.78 -1.6 2,520,277 + Pf1N1B4_2207 0.82 +0.3 2,520,277 + Pf1N1B4_2207 0.82 +0.9 2,520,277 + Pf1N1B4_2207 0.82 +0.3 2,520,278 - Pf1N1B4_2207 0.82 -0.3 2,520,296 + Pf1N1B4_2207 0.83 -0.2 2,520,296 + Pf1N1B4_2207 0.83 +0.9 2,520,296 + Pf1N1B4_2207 0.83 +0.3 2,520,296 + Pf1N1B4_2207 0.83 +1.1 2,520,296 + Pf1N1B4_2207 0.83 +1.6 2,520,296 + Pf1N1B4_2207 0.83 +0.5 2,520,296 + Pf1N1B4_2207 0.83 +0.4 2,520,314 + Pf1N1B4_2207 0.85 +0.0 2,520,314 + Pf1N1B4_2207 0.85 +0.6 2,520,314 + Pf1N1B4_2207 0.85 -0.1 2,520,314 + Pf1N1B4_2207 0.85 +0.6 2,520,314 + Pf1N1B4_2207 0.85 +0.8 2,520,388 + -0.4 2,520,419 + +0.7 2,520,419 + +1.7 2,520,419 + -0.1 2,520,419 + -0.1 2,520,419 + +1.4 2,520,419 + +0.3 2,520,419 + +0.0 2,520,419 + +0.7 2,520,420 - -0.7 2,520,420 - -0.7 2,520,420 - -0.7 2,520,461 + +0.6 2,520,461 + -0.5 2,520,650 + +0.7 2,520,650 + -1.0 2,520,651 - -1.5 2,520,651 - -1.1 2,520,651 - -1.3 2,520,651 - -1.7 2,521,232 + Pf1N1B4_2208 0.34 -1.7 2,521,232 + Pf1N1B4_2208 0.34 -0.5 2,521,232 + Pf1N1B4_2208 0.34 -1.4 2,521,232 + Pf1N1B4_2208 0.34 -0.7 2,521,232 + Pf1N1B4_2208 0.34 -0.7 2,521,233 - Pf1N1B4_2208 0.34 -1.7 2,521,233 - Pf1N1B4_2208 0.34 -2.3 2,521,460 + Pf1N1B4_2208 0.44 -0.3
Or see this region's nucleotide sequence