Experiment: nitrate 100 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2185 and Pf1N1B4_2186 overlap by 4 nucleotides Pf1N1B4_2186 and Pf1N1B4_2187 are separated by 16 nucleotides Pf1N1B4_2187 and Pf1N1B4_2188 overlap by 4 nucleotides
Pf1N1B4_2185 - Porphobilinogen deaminase (EC 2.5.1.61), at 2,497,352 to 2,498,293
_2185
Pf1N1B4_2186 - Uroporphyrinogen-III synthase (EC 4.2.1.75), at 2,498,290 to 2,499,057
_2186
Pf1N1B4_2187 - Uroporphyrinogen-III synthase (EC 4.2.1.75), at 2,499,074 to 2,500,228
_2187
Pf1N1B4_2188 - Uncharacterized protein EC-HemY, likely associated with heme metabolism based on gene clustering with hemC, hemD in Proteobacteria (unrelated to HemY-type PPO in GramPositives), at 2,500,225 to 2,501,463
_2188
Position (kb)
2499
2500
2501 Strain fitness (log2 ratio)
-1
0
1 at 2498.270 kb on + strand at 2498.270 kb on + strand at 2498.271 kb on - strand at 2498.271 kb on - strand at 2498.271 kb on - strand at 2498.271 kb on - strand at 2499.071 kb on + strand at 2500.881 kb on - strand, within Pf1N1B4_2188 at 2501.047 kb on + strand, within Pf1N1B4_2188 at 2501.122 kb on + strand, within Pf1N1B4_2188 at 2501.122 kb on + strand, within Pf1N1B4_2188
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate 100 mM remove 2,498,270 + -0.7 2,498,270 + +1.2 2,498,271 - -0.2 2,498,271 - -1.0 2,498,271 - +1.5 2,498,271 - +0.9 2,499,071 + -0.8 2,500,881 - Pf1N1B4_2188 0.53 +1.0 2,501,047 + Pf1N1B4_2188 0.66 +1.3 2,501,122 + Pf1N1B4_2188 0.72 +0.8 2,501,122 + Pf1N1B4_2188 0.72 -1.2
Or see this region's nucleotide sequence