Experiment: nitrate 100 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2184 and Pf1N1B4_2185 are separated by 126 nucleotides Pf1N1B4_2185 and Pf1N1B4_2186 overlap by 4 nucleotides Pf1N1B4_2186 and Pf1N1B4_2187 are separated by 16 nucleotides
Pf1N1B4_2184 - Autolysis response regulater LytR, at 2,496,479 to 2,497,225
_2184
Pf1N1B4_2185 - Porphobilinogen deaminase (EC 2.5.1.61), at 2,497,352 to 2,498,293
_2185
Pf1N1B4_2186 - Uroporphyrinogen-III synthase (EC 4.2.1.75), at 2,498,290 to 2,499,057
_2186
Pf1N1B4_2187 - Uroporphyrinogen-III synthase (EC 4.2.1.75), at 2,499,074 to 2,500,228
_2187
Position (kb)
2497
2498
2499 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2496.392 kb on + strand at 2496.393 kb on - strand at 2496.436 kb on + strand at 2496.478 kb on + strand at 2496.479 kb on - strand at 2496.479 kb on - strand at 2496.479 kb on - strand at 2496.554 kb on + strand, within Pf1N1B4_2184 at 2496.554 kb on + strand, within Pf1N1B4_2184 at 2496.554 kb on + strand, within Pf1N1B4_2184 at 2496.554 kb on + strand, within Pf1N1B4_2184 at 2496.554 kb on + strand, within Pf1N1B4_2184 at 2496.555 kb on - strand, within Pf1N1B4_2184 at 2496.555 kb on - strand, within Pf1N1B4_2184 at 2496.555 kb on - strand, within Pf1N1B4_2184 at 2496.555 kb on - strand, within Pf1N1B4_2184 at 2496.753 kb on + strand, within Pf1N1B4_2184 at 2496.804 kb on + strand, within Pf1N1B4_2184 at 2496.804 kb on + strand, within Pf1N1B4_2184 at 2496.804 kb on + strand, within Pf1N1B4_2184 at 2496.804 kb on + strand, within Pf1N1B4_2184 at 2496.805 kb on - strand, within Pf1N1B4_2184 at 2496.805 kb on - strand, within Pf1N1B4_2184 at 2496.816 kb on + strand, within Pf1N1B4_2184 at 2496.957 kb on - strand, within Pf1N1B4_2184 at 2496.957 kb on - strand, within Pf1N1B4_2184 at 2496.977 kb on + strand, within Pf1N1B4_2184 at 2496.977 kb on + strand, within Pf1N1B4_2184 at 2496.977 kb on + strand, within Pf1N1B4_2184 at 2496.977 kb on + strand, within Pf1N1B4_2184 at 2496.978 kb on - strand, within Pf1N1B4_2184 at 2496.978 kb on - strand, within Pf1N1B4_2184 at 2496.978 kb on - strand, within Pf1N1B4_2184 at 2496.978 kb on - strand, within Pf1N1B4_2184 at 2496.978 kb on - strand, within Pf1N1B4_2184 at 2496.978 kb on - strand, within Pf1N1B4_2184 at 2496.978 kb on - strand, within Pf1N1B4_2184 at 2496.978 kb on - strand, within Pf1N1B4_2184 at 2496.978 kb on - strand, within Pf1N1B4_2184 at 2497.243 kb on + strand at 2497.243 kb on + strand at 2497.243 kb on + strand at 2497.244 kb on - strand at 2497.244 kb on - strand at 2497.244 kb on - strand at 2497.244 kb on - strand at 2497.286 kb on + strand at 2497.286 kb on + strand at 2497.286 kb on + strand at 2497.287 kb on - strand at 2497.287 kb on - strand at 2497.316 kb on + strand at 2497.316 kb on + strand at 2497.317 kb on - strand at 2497.319 kb on + strand at 2497.319 kb on + strand at 2497.319 kb on + strand at 2497.332 kb on + strand at 2497.332 kb on + strand at 2497.332 kb on + strand at 2497.332 kb on + strand at 2497.332 kb on + strand at 2497.332 kb on + strand at 2498.270 kb on + strand at 2498.270 kb on + strand at 2498.271 kb on - strand at 2498.271 kb on - strand at 2498.271 kb on - strand at 2498.271 kb on - strand at 2499.071 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate 100 mM remove 2,496,392 + -1.2 2,496,393 - +0.7 2,496,436 + +0.3 2,496,478 + -1.0 2,496,479 - +0.0 2,496,479 - +0.6 2,496,479 - -0.3 2,496,554 + Pf1N1B4_2184 0.10 -0.6 2,496,554 + Pf1N1B4_2184 0.10 +0.1 2,496,554 + Pf1N1B4_2184 0.10 -0.3 2,496,554 + Pf1N1B4_2184 0.10 +1.3 2,496,554 + Pf1N1B4_2184 0.10 +3.0 2,496,555 - Pf1N1B4_2184 0.10 -2.0 2,496,555 - Pf1N1B4_2184 0.10 +1.2 2,496,555 - Pf1N1B4_2184 0.10 -0.8 2,496,555 - Pf1N1B4_2184 0.10 +0.3 2,496,753 + Pf1N1B4_2184 0.37 +0.4 2,496,804 + Pf1N1B4_2184 0.44 +1.5 2,496,804 + Pf1N1B4_2184 0.44 +0.9 2,496,804 + Pf1N1B4_2184 0.44 +0.3 2,496,804 + Pf1N1B4_2184 0.44 +0.3 2,496,805 - Pf1N1B4_2184 0.44 -1.8 2,496,805 - Pf1N1B4_2184 0.44 +0.3 2,496,816 + Pf1N1B4_2184 0.45 +1.6 2,496,957 - Pf1N1B4_2184 0.64 +0.1 2,496,957 - Pf1N1B4_2184 0.64 -0.9 2,496,977 + Pf1N1B4_2184 0.67 -0.5 2,496,977 + Pf1N1B4_2184 0.67 +1.3 2,496,977 + Pf1N1B4_2184 0.67 -0.2 2,496,977 + Pf1N1B4_2184 0.67 +0.2 2,496,978 - Pf1N1B4_2184 0.67 +0.3 2,496,978 - Pf1N1B4_2184 0.67 -0.4 2,496,978 - Pf1N1B4_2184 0.67 -1.8 2,496,978 - Pf1N1B4_2184 0.67 +0.3 2,496,978 - Pf1N1B4_2184 0.67 +2.3 2,496,978 - Pf1N1B4_2184 0.67 +0.6 2,496,978 - Pf1N1B4_2184 0.67 -0.2 2,496,978 - Pf1N1B4_2184 0.67 -1.5 2,496,978 - Pf1N1B4_2184 0.67 +0.9 2,497,243 + -0.7 2,497,243 + -0.7 2,497,243 + +1.3 2,497,244 - +1.1 2,497,244 - -1.2 2,497,244 - +1.3 2,497,244 - -0.2 2,497,286 + -0.1 2,497,286 + -0.2 2,497,286 + +1.9 2,497,287 - -1.2 2,497,287 - -0.7 2,497,316 + -0.7 2,497,316 + -0.7 2,497,317 - -2.4 2,497,319 + +0.9 2,497,319 + -1.0 2,497,319 + -0.9 2,497,332 + +1.3 2,497,332 + -0.7 2,497,332 + +0.6 2,497,332 + -1.0 2,497,332 + -2.0 2,497,332 + +0.8 2,498,270 + -0.7 2,498,270 + +1.2 2,498,271 - -0.2 2,498,271 - -1.0 2,498,271 - +1.5 2,498,271 - +0.9 2,499,071 + -0.8
Or see this region's nucleotide sequence