Experiment: nitrate 100 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1906 and Pf1N1B4_1907 are separated by 81 nucleotides Pf1N1B4_1907 and Pf1N1B4_1908 are separated by 173 nucleotides
Pf1N1B4_1906 - Shikimate 5-dehydrogenase I alpha (EC 1.1.1.25), at 2,180,656 to 2,181,477
_1906
Pf1N1B4_1907 - Coproporphyrinogen III oxidase, aerobic (EC 1.3.3.3), at 2,181,559 to 2,182,473
_1907
Pf1N1B4_1908 - Quinone oxidoreductase (EC 1.6.5.5), at 2,182,647 to 2,183,624
_1908
Position (kb)
2181
2182
2183 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2180.564 kb on + strand at 2180.564 kb on + strand at 2180.565 kb on - strand at 2180.649 kb on - strand at 2180.649 kb on - strand at 2180.726 kb on + strand at 2180.727 kb on - strand at 2180.831 kb on + strand, within Pf1N1B4_1906 at 2180.831 kb on + strand, within Pf1N1B4_1906 at 2180.832 kb on - strand, within Pf1N1B4_1906 at 2180.832 kb on - strand, within Pf1N1B4_1906 at 2180.832 kb on - strand, within Pf1N1B4_1906 at 2180.844 kb on - strand, within Pf1N1B4_1906 at 2180.844 kb on - strand, within Pf1N1B4_1906 at 2180.890 kb on + strand, within Pf1N1B4_1906 at 2180.890 kb on + strand, within Pf1N1B4_1906 at 2180.891 kb on - strand, within Pf1N1B4_1906 at 2181.121 kb on - strand, within Pf1N1B4_1906 at 2181.380 kb on + strand, within Pf1N1B4_1906 at 2181.381 kb on - strand, within Pf1N1B4_1906 at 2181.381 kb on - strand, within Pf1N1B4_1906 at 2181.381 kb on - strand, within Pf1N1B4_1906 at 2181.460 kb on + strand at 2181.461 kb on - strand at 2181.468 kb on - strand at 2181.479 kb on + strand at 2181.479 kb on + strand at 2181.479 kb on + strand at 2181.479 kb on + strand at 2181.480 kb on - strand at 2181.480 kb on - strand at 2181.480 kb on - strand at 2181.480 kb on - strand at 2181.480 kb on - strand at 2181.480 kb on - strand at 2181.480 kb on - strand at 2181.480 kb on - strand at 2181.480 kb on - strand at 2181.480 kb on - strand at 2181.480 kb on - strand at 2181.480 kb on - strand at 2181.495 kb on - strand at 2181.495 kb on - strand at 2181.495 kb on - strand at 2181.495 kb on - strand at 2182.499 kb on + strand at 2182.499 kb on + strand at 2182.499 kb on + strand at 2182.500 kb on - strand at 2182.513 kb on - strand at 2182.538 kb on - strand at 2182.538 kb on - strand at 2182.596 kb on + strand at 2182.597 kb on - strand at 2182.612 kb on - strand at 2182.619 kb on + strand at 2182.619 kb on + strand at 2182.619 kb on + strand at 2182.620 kb on - strand at 2182.620 kb on - strand at 2182.690 kb on + strand at 2182.691 kb on - strand at 2182.707 kb on + strand at 2182.782 kb on + strand, within Pf1N1B4_1908 at 2182.782 kb on + strand, within Pf1N1B4_1908 at 2182.782 kb on + strand, within Pf1N1B4_1908 at 2182.782 kb on + strand, within Pf1N1B4_1908 at 2182.782 kb on + strand, within Pf1N1B4_1908 at 2182.782 kb on + strand at 2182.782 kb on + strand, within Pf1N1B4_1908 at 2182.782 kb on + strand, within Pf1N1B4_1908 at 2182.782 kb on + strand, within Pf1N1B4_1908 at 2182.782 kb on + strand, within Pf1N1B4_1908 at 2182.782 kb on + strand, within Pf1N1B4_1908 at 2182.782 kb on + strand, within Pf1N1B4_1908 at 2182.782 kb on + strand, within Pf1N1B4_1908 at 2182.782 kb on + strand, within Pf1N1B4_1908 at 2182.782 kb on + strand, within Pf1N1B4_1908 at 2182.782 kb on + strand, within Pf1N1B4_1908 at 2182.783 kb on - strand, within Pf1N1B4_1908 at 2182.783 kb on - strand, within Pf1N1B4_1908 at 2182.783 kb on - strand, within Pf1N1B4_1908 at 2182.783 kb on - strand, within Pf1N1B4_1908 at 2182.783 kb on - strand, within Pf1N1B4_1908 at 2182.783 kb on - strand, within Pf1N1B4_1908 at 2182.783 kb on - strand, within Pf1N1B4_1908 at 2182.786 kb on - strand, within Pf1N1B4_1908 at 2182.800 kb on + strand, within Pf1N1B4_1908 at 2182.801 kb on - strand, within Pf1N1B4_1908 at 2182.929 kb on + strand, within Pf1N1B4_1908 at 2182.929 kb on + strand, within Pf1N1B4_1908 at 2182.929 kb on + strand, within Pf1N1B4_1908 at 2182.929 kb on + strand, within Pf1N1B4_1908 at 2182.930 kb on - strand, within Pf1N1B4_1908 at 2182.931 kb on + strand, within Pf1N1B4_1908 at 2182.931 kb on + strand, within Pf1N1B4_1908 at 2182.932 kb on - strand, within Pf1N1B4_1908 at 2182.932 kb on - strand, within Pf1N1B4_1908 at 2182.945 kb on + strand, within Pf1N1B4_1908 at 2182.945 kb on + strand, within Pf1N1B4_1908 at 2182.945 kb on + strand, within Pf1N1B4_1908 at 2182.945 kb on + strand, within Pf1N1B4_1908 at 2182.946 kb on - strand, within Pf1N1B4_1908 at 2182.946 kb on - strand, within Pf1N1B4_1908 at 2182.966 kb on + strand, within Pf1N1B4_1908 at 2182.966 kb on + strand, within Pf1N1B4_1908 at 2182.966 kb on + strand, within Pf1N1B4_1908 at 2182.966 kb on + strand, within Pf1N1B4_1908 at 2183.052 kb on + strand, within Pf1N1B4_1908 at 2183.052 kb on + strand, within Pf1N1B4_1908 at 2183.052 kb on + strand, within Pf1N1B4_1908 at 2183.052 kb on + strand, within Pf1N1B4_1908 at 2183.052 kb on + strand, within Pf1N1B4_1908 at 2183.052 kb on + strand, within Pf1N1B4_1908 at 2183.052 kb on + strand, within Pf1N1B4_1908 at 2183.052 kb on + strand, within Pf1N1B4_1908 at 2183.052 kb on + strand, within Pf1N1B4_1908 at 2183.052 kb on + strand, within Pf1N1B4_1908 at 2183.053 kb on - strand, within Pf1N1B4_1908 at 2183.053 kb on - strand, within Pf1N1B4_1908 at 2183.053 kb on - strand, within Pf1N1B4_1908 at 2183.053 kb on - strand, within Pf1N1B4_1908 at 2183.053 kb on - strand, within Pf1N1B4_1908 at 2183.220 kb on + strand at 2183.220 kb on + strand, within Pf1N1B4_1908 at 2183.286 kb on + strand, within Pf1N1B4_1908 at 2183.286 kb on + strand, within Pf1N1B4_1908 at 2183.286 kb on + strand, within Pf1N1B4_1908 at 2183.286 kb on + strand, within Pf1N1B4_1908 at 2183.286 kb on + strand, within Pf1N1B4_1908 at 2183.287 kb on - strand, within Pf1N1B4_1908 at 2183.287 kb on - strand, within Pf1N1B4_1908 at 2183.287 kb on - strand, within Pf1N1B4_1908 at 2183.287 kb on - strand, within Pf1N1B4_1908 at 2183.380 kb on + strand, within Pf1N1B4_1908 at 2183.381 kb on - strand, within Pf1N1B4_1908 at 2183.424 kb on + strand, within Pf1N1B4_1908 at 2183.424 kb on + strand, within Pf1N1B4_1908 at 2183.424 kb on + strand, within Pf1N1B4_1908 at 2183.424 kb on + strand, within Pf1N1B4_1908 at 2183.424 kb on + strand at 2183.425 kb on - strand, within Pf1N1B4_1908 at 2183.451 kb on + strand, within Pf1N1B4_1908 at 2183.451 kb on + strand, within Pf1N1B4_1908
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate 100 mM remove 2,180,564 + -0.8 2,180,564 + -0.8 2,180,565 - -0.2 2,180,649 - -1.1 2,180,649 - -0.2 2,180,726 + +1.4 2,180,727 - +0.4 2,180,831 + Pf1N1B4_1906 0.21 -0.6 2,180,831 + Pf1N1B4_1906 0.21 +1.0 2,180,832 - Pf1N1B4_1906 0.21 +0.2 2,180,832 - Pf1N1B4_1906 0.21 +0.1 2,180,832 - Pf1N1B4_1906 0.21 +0.4 2,180,844 - Pf1N1B4_1906 0.23 -0.6 2,180,844 - Pf1N1B4_1906 0.23 +1.1 2,180,890 + Pf1N1B4_1906 0.28 -0.3 2,180,890 + Pf1N1B4_1906 0.28 -0.6 2,180,891 - Pf1N1B4_1906 0.29 +0.3 2,181,121 - Pf1N1B4_1906 0.57 -0.2 2,181,380 + Pf1N1B4_1906 0.88 -0.1 2,181,381 - Pf1N1B4_1906 0.88 -0.2 2,181,381 - Pf1N1B4_1906 0.88 +0.1 2,181,381 - Pf1N1B4_1906 0.88 -1.4 2,181,460 + -0.2 2,181,461 - -1.4 2,181,468 - -0.3 2,181,479 + +0.2 2,181,479 + -1.2 2,181,479 + -0.2 2,181,479 + -0.1 2,181,480 - +0.3 2,181,480 - -1.2 2,181,480 - -0.3 2,181,480 - +0.1 2,181,480 - +1.4 2,181,480 - -2.3 2,181,480 - -1.8 2,181,480 - +0.5 2,181,480 - -0.5 2,181,480 - +1.0 2,181,480 - +0.8 2,181,480 - -0.5 2,181,495 - +0.9 2,181,495 - +0.5 2,181,495 - +0.4 2,181,495 - +0.1 2,182,499 + -0.3 2,182,499 + -0.9 2,182,499 + +0.4 2,182,500 - -1.7 2,182,513 - +0.1 2,182,538 - +1.2 2,182,538 - -0.9 2,182,596 + +0.2 2,182,597 - -0.3 2,182,612 - +0.4 2,182,619 + -1.1 2,182,619 + -0.2 2,182,619 + -1.2 2,182,620 - -1.2 2,182,620 - +0.2 2,182,690 + -0.0 2,182,691 - -1.6 2,182,707 + -0.4 2,182,782 + Pf1N1B4_1908 0.14 +0.9 2,182,782 + Pf1N1B4_1908 0.14 +0.4 2,182,782 + Pf1N1B4_1908 0.14 -0.4 2,182,782 + Pf1N1B4_1908 0.14 +1.9 2,182,782 + Pf1N1B4_1908 0.14 -0.8 2,182,782 + -0.2 2,182,782 + Pf1N1B4_1908 0.14 -1.9 2,182,782 + Pf1N1B4_1908 0.14 +0.5 2,182,782 + Pf1N1B4_1908 0.14 +0.4 2,182,782 + Pf1N1B4_1908 0.14 +0.8 2,182,782 + Pf1N1B4_1908 0.14 -0.4 2,182,782 + Pf1N1B4_1908 0.14 +0.1 2,182,782 + Pf1N1B4_1908 0.14 +0.8 2,182,782 + Pf1N1B4_1908 0.14 +0.4 2,182,782 + Pf1N1B4_1908 0.14 +0.2 2,182,782 + Pf1N1B4_1908 0.14 +0.7 2,182,783 - Pf1N1B4_1908 0.14 +0.4 2,182,783 - Pf1N1B4_1908 0.14 -0.8 2,182,783 - Pf1N1B4_1908 0.14 -1.0 2,182,783 - Pf1N1B4_1908 0.14 -0.1 2,182,783 - Pf1N1B4_1908 0.14 +0.9 2,182,783 - Pf1N1B4_1908 0.14 +0.1 2,182,783 - Pf1N1B4_1908 0.14 +0.5 2,182,786 - Pf1N1B4_1908 0.14 -0.1 2,182,800 + Pf1N1B4_1908 0.16 -1.1 2,182,801 - Pf1N1B4_1908 0.16 +0.6 2,182,929 + Pf1N1B4_1908 0.29 -0.4 2,182,929 + Pf1N1B4_1908 0.29 +0.4 2,182,929 + Pf1N1B4_1908 0.29 +0.4 2,182,929 + Pf1N1B4_1908 0.29 -0.0 2,182,930 - Pf1N1B4_1908 0.29 -1.1 2,182,931 + Pf1N1B4_1908 0.29 -1.9 2,182,931 + Pf1N1B4_1908 0.29 +1.4 2,182,932 - Pf1N1B4_1908 0.29 +0.3 2,182,932 - Pf1N1B4_1908 0.29 -0.8 2,182,945 + Pf1N1B4_1908 0.30 -1.7 2,182,945 + Pf1N1B4_1908 0.30 +1.4 2,182,945 + Pf1N1B4_1908 0.30 +1.1 2,182,945 + Pf1N1B4_1908 0.30 -0.9 2,182,946 - Pf1N1B4_1908 0.31 -0.0 2,182,946 - Pf1N1B4_1908 0.31 +1.4 2,182,966 + Pf1N1B4_1908 0.33 +0.4 2,182,966 + Pf1N1B4_1908 0.33 -2.5 2,182,966 + Pf1N1B4_1908 0.33 -0.0 2,182,966 + Pf1N1B4_1908 0.33 +1.0 2,183,052 + Pf1N1B4_1908 0.41 -1.5 2,183,052 + Pf1N1B4_1908 0.41 +0.2 2,183,052 + Pf1N1B4_1908 0.41 +1.1 2,183,052 + Pf1N1B4_1908 0.41 -0.9 2,183,052 + Pf1N1B4_1908 0.41 +0.3 2,183,052 + Pf1N1B4_1908 0.41 +0.5 2,183,052 + Pf1N1B4_1908 0.41 -1.1 2,183,052 + Pf1N1B4_1908 0.41 +1.4 2,183,052 + Pf1N1B4_1908 0.41 -0.9 2,183,052 + Pf1N1B4_1908 0.41 -0.3 2,183,053 - Pf1N1B4_1908 0.42 +1.4 2,183,053 - Pf1N1B4_1908 0.42 -0.2 2,183,053 - Pf1N1B4_1908 0.42 -2.5 2,183,053 - Pf1N1B4_1908 0.42 +1.4 2,183,053 - Pf1N1B4_1908 0.42 -0.6 2,183,220 + +1.4 2,183,220 + Pf1N1B4_1908 0.59 +0.1 2,183,286 + Pf1N1B4_1908 0.65 -0.4 2,183,286 + Pf1N1B4_1908 0.65 +0.6 2,183,286 + Pf1N1B4_1908 0.65 -0.0 2,183,286 + Pf1N1B4_1908 0.65 -0.5 2,183,286 + Pf1N1B4_1908 0.65 -1.8 2,183,287 - Pf1N1B4_1908 0.65 -1.5 2,183,287 - Pf1N1B4_1908 0.65 -1.2 2,183,287 - Pf1N1B4_1908 0.65 -0.6 2,183,287 - Pf1N1B4_1908 0.65 -0.1 2,183,380 + Pf1N1B4_1908 0.75 +0.4 2,183,381 - Pf1N1B4_1908 0.75 +0.1 2,183,424 + Pf1N1B4_1908 0.79 -1.8 2,183,424 + Pf1N1B4_1908 0.79 -1.2 2,183,424 + Pf1N1B4_1908 0.79 +1.8 2,183,424 + Pf1N1B4_1908 0.79 -0.5 2,183,424 + +0.4 2,183,425 - Pf1N1B4_1908 0.80 +0.1 2,183,451 + Pf1N1B4_1908 0.82 +0.4 2,183,451 + Pf1N1B4_1908 0.82 -2.5
Or see this region's nucleotide sequence