Experiment: nitrate 100 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1531 and Pf1N1B4_1532 are separated by 181 nucleotides Pf1N1B4_1532 and Pf1N1B4_1533 are separated by 246 nucleotides
Pf1N1B4_1531 - Permeases of the major facilitator superfamily, at 1,762,394 to 1,763,668
_1531
Pf1N1B4_1532 - Uroporphyrinogen III decarboxylase (EC 4.1.1.37), at 1,763,850 to 1,764,917
_1532
Pf1N1B4_1533 - Glutamate synthase [NADPH] small chain (EC 1.4.1.13), at 1,765,164 to 1,766,582
_1533
Position (kb)
1763
1764
1765 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1762.892 kb on + strand, within Pf1N1B4_1531 at 1762.892 kb on + strand, within Pf1N1B4_1531 at 1762.892 kb on + strand, within Pf1N1B4_1531 at 1762.892 kb on + strand, within Pf1N1B4_1531 at 1762.892 kb on + strand, within Pf1N1B4_1531 at 1762.945 kb on - strand, within Pf1N1B4_1531 at 1763.177 kb on + strand, within Pf1N1B4_1531 at 1763.177 kb on + strand, within Pf1N1B4_1531 at 1763.177 kb on + strand, within Pf1N1B4_1531 at 1763.177 kb on + strand, within Pf1N1B4_1531 at 1763.178 kb on - strand, within Pf1N1B4_1531 at 1763.178 kb on - strand, within Pf1N1B4_1531 at 1763.249 kb on + strand, within Pf1N1B4_1531 at 1763.249 kb on + strand, within Pf1N1B4_1531 at 1763.249 kb on + strand, within Pf1N1B4_1531 at 1763.249 kb on + strand, within Pf1N1B4_1531 at 1763.250 kb on - strand, within Pf1N1B4_1531 at 1763.264 kb on + strand, within Pf1N1B4_1531 at 1763.264 kb on + strand, within Pf1N1B4_1531 at 1763.264 kb on + strand, within Pf1N1B4_1531 at 1763.264 kb on + strand, within Pf1N1B4_1531 at 1763.264 kb on + strand, within Pf1N1B4_1531 at 1763.264 kb on + strand, within Pf1N1B4_1531 at 1763.264 kb on + strand, within Pf1N1B4_1531 at 1763.264 kb on + strand, within Pf1N1B4_1531 at 1763.265 kb on - strand, within Pf1N1B4_1531 at 1763.265 kb on - strand, within Pf1N1B4_1531 at 1763.265 kb on - strand, within Pf1N1B4_1531 at 1763.265 kb on - strand, within Pf1N1B4_1531 at 1763.443 kb on + strand, within Pf1N1B4_1531 at 1763.444 kb on - strand, within Pf1N1B4_1531 at 1763.444 kb on - strand, within Pf1N1B4_1531 at 1763.495 kb on + strand, within Pf1N1B4_1531 at 1763.495 kb on + strand, within Pf1N1B4_1531 at 1763.496 kb on - strand, within Pf1N1B4_1531 at 1763.496 kb on - strand, within Pf1N1B4_1531 at 1763.496 kb on - strand, within Pf1N1B4_1531 at 1763.507 kb on + strand, within Pf1N1B4_1531 at 1763.508 kb on - strand, within Pf1N1B4_1531 at 1763.508 kb on - strand, within Pf1N1B4_1531 at 1763.508 kb on - strand, within Pf1N1B4_1531 at 1763.510 kb on + strand, within Pf1N1B4_1531 at 1763.587 kb on + strand at 1763.731 kb on + strand at 1763.731 kb on + strand at 1763.732 kb on - strand at 1763.821 kb on + strand at 1763.837 kb on + strand at 1763.860 kb on + strand at 1763.861 kb on - strand at 1763.861 kb on - strand at 1765.160 kb on - strand at 1765.160 kb on - strand at 1765.177 kb on + strand at 1765.178 kb on - strand at 1765.295 kb on - strand at 1765.664 kb on + strand, within Pf1N1B4_1533 at 1765.664 kb on + strand, within Pf1N1B4_1533 at 1765.664 kb on + strand, within Pf1N1B4_1533 at 1765.664 kb on + strand, within Pf1N1B4_1533 at 1765.664 kb on + strand, within Pf1N1B4_1533 at 1765.664 kb on + strand, within Pf1N1B4_1533 at 1765.665 kb on - strand, within Pf1N1B4_1533 at 1765.665 kb on - strand, within Pf1N1B4_1533 at 1765.665 kb on - strand, within Pf1N1B4_1533 at 1765.665 kb on - strand, within Pf1N1B4_1533 at 1765.665 kb on - strand, within Pf1N1B4_1533 at 1765.843 kb on + strand, within Pf1N1B4_1533 at 1765.843 kb on + strand, within Pf1N1B4_1533 at 1765.843 kb on + strand, within Pf1N1B4_1533 at 1765.843 kb on + strand, within Pf1N1B4_1533 at 1765.843 kb on + strand, within Pf1N1B4_1533 at 1765.843 kb on + strand, within Pf1N1B4_1533 at 1765.843 kb on + strand, within Pf1N1B4_1533 at 1765.843 kb on + strand, within Pf1N1B4_1533 at 1765.844 kb on - strand, within Pf1N1B4_1533 at 1765.844 kb on - strand, within Pf1N1B4_1533 at 1765.844 kb on - strand, within Pf1N1B4_1533 at 1765.844 kb on - strand, within Pf1N1B4_1533 at 1765.844 kb on - strand, within Pf1N1B4_1533 at 1765.844 kb on - strand, within Pf1N1B4_1533 at 1765.844 kb on - strand, within Pf1N1B4_1533 at 1765.844 kb on - strand, within Pf1N1B4_1533 at 1765.844 kb on - strand, within Pf1N1B4_1533 at 1765.844 kb on - strand, within Pf1N1B4_1533 at 1765.844 kb on - strand, within Pf1N1B4_1533 at 1765.844 kb on - strand, within Pf1N1B4_1533 at 1765.849 kb on + strand, within Pf1N1B4_1533 at 1765.849 kb on + strand, within Pf1N1B4_1533 at 1765.849 kb on + strand, within Pf1N1B4_1533 at 1765.849 kb on + strand, within Pf1N1B4_1533 at 1765.849 kb on + strand, within Pf1N1B4_1533 at 1765.849 kb on + strand, within Pf1N1B4_1533 at 1765.849 kb on + strand, within Pf1N1B4_1533 at 1765.849 kb on + strand, within Pf1N1B4_1533 at 1765.849 kb on + strand, within Pf1N1B4_1533 at 1765.849 kb on + strand, within Pf1N1B4_1533 at 1765.849 kb on + strand, within Pf1N1B4_1533 at 1765.849 kb on + strand, within Pf1N1B4_1533 at 1765.850 kb on - strand, within Pf1N1B4_1533 at 1765.850 kb on - strand, within Pf1N1B4_1533 at 1765.850 kb on - strand, within Pf1N1B4_1533 at 1765.850 kb on - strand, within Pf1N1B4_1533 at 1765.850 kb on - strand, within Pf1N1B4_1533 at 1765.850 kb on - strand, within Pf1N1B4_1533 at 1765.850 kb on - strand at 1765.850 kb on - strand, within Pf1N1B4_1533 at 1765.879 kb on + strand, within Pf1N1B4_1533 at 1765.879 kb on + strand, within Pf1N1B4_1533
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate 100 mM remove 1,762,892 + Pf1N1B4_1531 0.39 +1.5 1,762,892 + Pf1N1B4_1531 0.39 +0.2 1,762,892 + Pf1N1B4_1531 0.39 +0.3 1,762,892 + Pf1N1B4_1531 0.39 -0.3 1,762,892 + Pf1N1B4_1531 0.39 -0.3 1,762,945 - Pf1N1B4_1531 0.43 -1.4 1,763,177 + Pf1N1B4_1531 0.61 +2.4 1,763,177 + Pf1N1B4_1531 0.61 -1.2 1,763,177 + Pf1N1B4_1531 0.61 -0.5 1,763,177 + Pf1N1B4_1531 0.61 -0.3 1,763,178 - Pf1N1B4_1531 0.61 -0.1 1,763,178 - Pf1N1B4_1531 0.61 +1.3 1,763,249 + Pf1N1B4_1531 0.67 +0.5 1,763,249 + Pf1N1B4_1531 0.67 +0.8 1,763,249 + Pf1N1B4_1531 0.67 +0.9 1,763,249 + Pf1N1B4_1531 0.67 -0.3 1,763,250 - Pf1N1B4_1531 0.67 -0.4 1,763,264 + Pf1N1B4_1531 0.68 +1.9 1,763,264 + Pf1N1B4_1531 0.68 +1.3 1,763,264 + Pf1N1B4_1531 0.68 -0.7 1,763,264 + Pf1N1B4_1531 0.68 +2.2 1,763,264 + Pf1N1B4_1531 0.68 -1.9 1,763,264 + Pf1N1B4_1531 0.68 -0.5 1,763,264 + Pf1N1B4_1531 0.68 -1.9 1,763,264 + Pf1N1B4_1531 0.68 -0.7 1,763,265 - Pf1N1B4_1531 0.68 +1.3 1,763,265 - Pf1N1B4_1531 0.68 -0.8 1,763,265 - Pf1N1B4_1531 0.68 -0.3 1,763,265 - Pf1N1B4_1531 0.68 -0.0 1,763,443 + Pf1N1B4_1531 0.82 -2.5 1,763,444 - Pf1N1B4_1531 0.82 -0.3 1,763,444 - Pf1N1B4_1531 0.82 +0.1 1,763,495 + Pf1N1B4_1531 0.86 -2.5 1,763,495 + Pf1N1B4_1531 0.86 -1.1 1,763,496 - Pf1N1B4_1531 0.86 -0.4 1,763,496 - Pf1N1B4_1531 0.86 -0.4 1,763,496 - Pf1N1B4_1531 0.86 +0.8 1,763,507 + Pf1N1B4_1531 0.87 -0.2 1,763,508 - Pf1N1B4_1531 0.87 +2.0 1,763,508 - Pf1N1B4_1531 0.87 +0.8 1,763,508 - Pf1N1B4_1531 0.87 -0.1 1,763,510 + Pf1N1B4_1531 0.88 -0.9 1,763,587 + +0.3 1,763,731 + +1.9 1,763,731 + +0.6 1,763,732 - -0.7 1,763,821 + -1.9 1,763,837 + +0.1 1,763,860 + +0.3 1,763,861 - -1.3 1,763,861 - -0.5 1,765,160 - +0.3 1,765,160 - -0.4 1,765,177 + +1.3 1,765,178 - +0.1 1,765,295 - +1.3 1,765,664 + Pf1N1B4_1533 0.35 +1.3 1,765,664 + Pf1N1B4_1533 0.35 -1.5 1,765,664 + Pf1N1B4_1533 0.35 -0.6 1,765,664 + Pf1N1B4_1533 0.35 -0.5 1,765,664 + Pf1N1B4_1533 0.35 +1.3 1,765,664 + Pf1N1B4_1533 0.35 +2.5 1,765,665 - Pf1N1B4_1533 0.35 -0.3 1,765,665 - Pf1N1B4_1533 0.35 +0.7 1,765,665 - Pf1N1B4_1533 0.35 -0.1 1,765,665 - Pf1N1B4_1533 0.35 -0.1 1,765,665 - Pf1N1B4_1533 0.35 +1.3 1,765,843 + Pf1N1B4_1533 0.48 -1.4 1,765,843 + Pf1N1B4_1533 0.48 -0.4 1,765,843 + Pf1N1B4_1533 0.48 -0.7 1,765,843 + Pf1N1B4_1533 0.48 +0.3 1,765,843 + Pf1N1B4_1533 0.48 -0.9 1,765,843 + Pf1N1B4_1533 0.48 +1.3 1,765,843 + Pf1N1B4_1533 0.48 +0.6 1,765,843 + Pf1N1B4_1533 0.48 -1.7 1,765,844 - Pf1N1B4_1533 0.48 -1.1 1,765,844 - Pf1N1B4_1533 0.48 +0.7 1,765,844 - Pf1N1B4_1533 0.48 -0.5 1,765,844 - Pf1N1B4_1533 0.48 -1.3 1,765,844 - Pf1N1B4_1533 0.48 -0.3 1,765,844 - Pf1N1B4_1533 0.48 +0.2 1,765,844 - Pf1N1B4_1533 0.48 +0.8 1,765,844 - Pf1N1B4_1533 0.48 +0.5 1,765,844 - Pf1N1B4_1533 0.48 +1.3 1,765,844 - Pf1N1B4_1533 0.48 +0.3 1,765,844 - Pf1N1B4_1533 0.48 -0.4 1,765,844 - Pf1N1B4_1533 0.48 -1.1 1,765,849 + Pf1N1B4_1533 0.48 +1.3 1,765,849 + Pf1N1B4_1533 0.48 -0.1 1,765,849 + Pf1N1B4_1533 0.48 +1.7 1,765,849 + Pf1N1B4_1533 0.48 +0.3 1,765,849 + Pf1N1B4_1533 0.48 -1.5 1,765,849 + Pf1N1B4_1533 0.48 +0.3 1,765,849 + Pf1N1B4_1533 0.48 -0.9 1,765,849 + Pf1N1B4_1533 0.48 +0.6 1,765,849 + Pf1N1B4_1533 0.48 +0.7 1,765,849 + Pf1N1B4_1533 0.48 +0.7 1,765,849 + Pf1N1B4_1533 0.48 -2.4 1,765,849 + Pf1N1B4_1533 0.48 +0.9 1,765,850 - Pf1N1B4_1533 0.48 -0.1 1,765,850 - Pf1N1B4_1533 0.48 -1.6 1,765,850 - Pf1N1B4_1533 0.48 -1.9 1,765,850 - Pf1N1B4_1533 0.48 -1.9 1,765,850 - Pf1N1B4_1533 0.48 +0.3 1,765,850 - Pf1N1B4_1533 0.48 +0.1 1,765,850 - +0.3 1,765,850 - Pf1N1B4_1533 0.48 +1.7 1,765,879 + Pf1N1B4_1533 0.50 +0.6 1,765,879 + Pf1N1B4_1533 0.50 -0.6
Or see this region's nucleotide sequence