Experiment: nitrate 100 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1444 and Pf1N1B4_1445 are separated by 228 nucleotides Pf1N1B4_1445 and Pf1N1B4_1446 overlap by 32 nucleotides Pf1N1B4_1446 and Pf1N1B4_1447 are separated by 184 nucleotides
Pf1N1B4_1444 - ADP-ribose pyrophosphatase (EC 3.6.1.13), at 1,655,552 to 1,656,169
_1444
Pf1N1B4_1445 - lipoprotein, putative, at 1,656,398 to 1,657,144
_1445
Pf1N1B4_1446 - Predicted hydroxymethylpyrimidine transporter CytX, at 1,657,113 to 1,658,366
_1446
Pf1N1B4_1447 - Hydroxymethylpyrimidine phosphate synthase ThiC (EC 4.1.99.17), at 1,658,551 to 1,660,440
_1447
Position (kb)
1657
1658
1659 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1656.215 kb on - strand at 1656.313 kb on + strand at 1656.313 kb on + strand at 1656.313 kb on + strand at 1656.313 kb on + strand at 1656.313 kb on + strand at 1656.313 kb on + strand at 1656.313 kb on + strand at 1656.314 kb on - strand at 1656.314 kb on - strand at 1656.317 kb on + strand at 1656.317 kb on + strand at 1656.318 kb on - strand at 1656.318 kb on - strand at 1656.318 kb on - strand at 1656.318 kb on - strand at 1656.318 kb on - strand at 1656.322 kb on + strand at 1656.322 kb on + strand at 1656.322 kb on + strand at 1656.387 kb on - strand at 1656.477 kb on - strand, within Pf1N1B4_1445 at 1656.477 kb on - strand, within Pf1N1B4_1445 at 1656.496 kb on + strand, within Pf1N1B4_1445 at 1656.496 kb on + strand, within Pf1N1B4_1445 at 1656.654 kb on + strand, within Pf1N1B4_1445 at 1656.654 kb on + strand, within Pf1N1B4_1445 at 1656.655 kb on - strand, within Pf1N1B4_1445 at 1656.655 kb on - strand, within Pf1N1B4_1445 at 1656.655 kb on - strand, within Pf1N1B4_1445 at 1656.655 kb on - strand at 1656.719 kb on + strand, within Pf1N1B4_1445 at 1656.719 kb on + strand, within Pf1N1B4_1445 at 1656.719 kb on + strand, within Pf1N1B4_1445 at 1656.719 kb on + strand, within Pf1N1B4_1445 at 1656.720 kb on - strand, within Pf1N1B4_1445 at 1656.720 kb on - strand, within Pf1N1B4_1445 at 1656.720 kb on - strand, within Pf1N1B4_1445 at 1656.720 kb on - strand, within Pf1N1B4_1445 at 1656.735 kb on + strand, within Pf1N1B4_1445 at 1656.735 kb on + strand, within Pf1N1B4_1445 at 1656.735 kb on + strand, within Pf1N1B4_1445 at 1656.736 kb on - strand, within Pf1N1B4_1445 at 1656.736 kb on - strand, within Pf1N1B4_1445 at 1656.736 kb on - strand, within Pf1N1B4_1445 at 1656.736 kb on - strand, within Pf1N1B4_1445 at 1656.736 kb on - strand, within Pf1N1B4_1445 at 1656.779 kb on + strand, within Pf1N1B4_1445 at 1656.780 kb on - strand, within Pf1N1B4_1445 at 1656.780 kb on - strand, within Pf1N1B4_1445 at 1656.780 kb on - strand, within Pf1N1B4_1445 at 1656.780 kb on - strand, within Pf1N1B4_1445 at 1656.830 kb on + strand at 1656.830 kb on + strand, within Pf1N1B4_1445 at 1656.830 kb on + strand, within Pf1N1B4_1445 at 1656.830 kb on + strand, within Pf1N1B4_1445 at 1656.830 kb on + strand, within Pf1N1B4_1445 at 1656.830 kb on + strand, within Pf1N1B4_1445 at 1656.830 kb on + strand, within Pf1N1B4_1445 at 1656.830 kb on + strand, within Pf1N1B4_1445 at 1656.831 kb on - strand, within Pf1N1B4_1445 at 1656.831 kb on - strand, within Pf1N1B4_1445 at 1656.831 kb on - strand, within Pf1N1B4_1445 at 1656.831 kb on - strand, within Pf1N1B4_1445 at 1656.831 kb on - strand, within Pf1N1B4_1445 at 1656.831 kb on - strand, within Pf1N1B4_1445 at 1656.831 kb on - strand, within Pf1N1B4_1445 at 1656.847 kb on + strand at 1656.847 kb on + strand, within Pf1N1B4_1445 at 1656.847 kb on + strand, within Pf1N1B4_1445 at 1656.848 kb on - strand, within Pf1N1B4_1445 at 1656.848 kb on - strand, within Pf1N1B4_1445 at 1656.848 kb on - strand, within Pf1N1B4_1445 at 1656.848 kb on - strand, within Pf1N1B4_1445 at 1656.848 kb on - strand at 1656.848 kb on - strand, within Pf1N1B4_1445 at 1657.014 kb on - strand, within Pf1N1B4_1445 at 1657.034 kb on + strand, within Pf1N1B4_1445 at 1657.034 kb on + strand, within Pf1N1B4_1445 at 1657.035 kb on - strand, within Pf1N1B4_1445 at 1657.035 kb on - strand, within Pf1N1B4_1445 at 1657.035 kb on - strand at 1657.139 kb on - strand at 1657.237 kb on + strand at 1657.238 kb on - strand at 1657.238 kb on - strand at 1657.238 kb on - strand at 1657.238 kb on - strand at 1657.238 kb on - strand at 1657.296 kb on + strand, within Pf1N1B4_1446 at 1657.378 kb on - strand, within Pf1N1B4_1446 at 1657.630 kb on + strand at 1657.630 kb on + strand, within Pf1N1B4_1446 at 1657.868 kb on + strand, within Pf1N1B4_1446 at 1657.869 kb on - strand, within Pf1N1B4_1446 at 1657.869 kb on - strand, within Pf1N1B4_1446 at 1657.869 kb on - strand, within Pf1N1B4_1446 at 1657.869 kb on - strand, within Pf1N1B4_1446 at 1657.869 kb on - strand, within Pf1N1B4_1446 at 1657.893 kb on - strand, within Pf1N1B4_1446 at 1658.383 kb on + strand at 1658.383 kb on + strand at 1658.383 kb on + strand at 1658.384 kb on - strand at 1658.384 kb on - strand at 1658.384 kb on - strand at 1658.384 kb on - strand at 1658.384 kb on - strand at 1658.412 kb on + strand at 1658.412 kb on + strand at 1658.413 kb on - strand at 1658.413 kb on - strand at 1658.413 kb on - strand at 1658.413 kb on - strand at 1658.413 kb on - strand at 1658.565 kb on - strand at 1658.565 kb on - strand at 1658.765 kb on + strand, within Pf1N1B4_1447 at 1658.766 kb on - strand, within Pf1N1B4_1447 at 1658.766 kb on - strand, within Pf1N1B4_1447 at 1658.909 kb on + strand, within Pf1N1B4_1447 at 1658.909 kb on + strand, within Pf1N1B4_1447 at 1658.910 kb on - strand, within Pf1N1B4_1447 at 1658.910 kb on - strand, within Pf1N1B4_1447 at 1658.910 kb on - strand, within Pf1N1B4_1447 at 1658.910 kb on - strand, within Pf1N1B4_1447 at 1658.910 kb on - strand at 1658.910 kb on - strand, within Pf1N1B4_1447 at 1658.910 kb on - strand, within Pf1N1B4_1447 at 1658.975 kb on + strand, within Pf1N1B4_1447 at 1658.975 kb on + strand, within Pf1N1B4_1447 at 1658.976 kb on - strand, within Pf1N1B4_1447 at 1659.080 kb on + strand, within Pf1N1B4_1447 at 1659.081 kb on - strand, within Pf1N1B4_1447 at 1659.081 kb on - strand, within Pf1N1B4_1447 at 1659.175 kb on + strand, within Pf1N1B4_1447 at 1659.175 kb on + strand, within Pf1N1B4_1447 at 1659.175 kb on + strand, within Pf1N1B4_1447 at 1659.175 kb on + strand, within Pf1N1B4_1447 at 1659.175 kb on + strand, within Pf1N1B4_1447 at 1659.176 kb on - strand, within Pf1N1B4_1447 at 1659.176 kb on - strand, within Pf1N1B4_1447 at 1659.176 kb on - strand, within Pf1N1B4_1447 at 1659.176 kb on - strand, within Pf1N1B4_1447 at 1659.176 kb on - strand, within Pf1N1B4_1447 at 1659.176 kb on - strand, within Pf1N1B4_1447 at 1659.176 kb on - strand, within Pf1N1B4_1447 at 1659.302 kb on + strand, within Pf1N1B4_1447 at 1659.302 kb on + strand, within Pf1N1B4_1447 at 1659.342 kb on - strand, within Pf1N1B4_1447 at 1659.357 kb on + strand, within Pf1N1B4_1447 at 1659.357 kb on + strand, within Pf1N1B4_1447 at 1659.358 kb on - strand, within Pf1N1B4_1447
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate 100 mM remove 1,656,215 - -0.2 1,656,313 + +0.5 1,656,313 + -0.6 1,656,313 + +0.9 1,656,313 + -0.4 1,656,313 + -0.8 1,656,313 + -0.6 1,656,313 + -0.8 1,656,314 - +2.2 1,656,314 - -0.8 1,656,317 + +0.6 1,656,317 + +0.8 1,656,318 - +0.7 1,656,318 - +0.1 1,656,318 - -0.1 1,656,318 - +0.1 1,656,318 - +1.6 1,656,322 + +0.7 1,656,322 + -0.4 1,656,322 + -2.1 1,656,387 - -1.4 1,656,477 - Pf1N1B4_1445 0.11 +0.5 1,656,477 - Pf1N1B4_1445 0.11 +0.2 1,656,496 + Pf1N1B4_1445 0.13 -0.8 1,656,496 + Pf1N1B4_1445 0.13 -0.9 1,656,654 + Pf1N1B4_1445 0.34 -2.1 1,656,654 + Pf1N1B4_1445 0.34 -0.0 1,656,655 - Pf1N1B4_1445 0.34 -0.8 1,656,655 - Pf1N1B4_1445 0.34 +1.2 1,656,655 - Pf1N1B4_1445 0.34 -1.1 1,656,655 - +1.2 1,656,719 + Pf1N1B4_1445 0.43 +0.1 1,656,719 + Pf1N1B4_1445 0.43 +0.7 1,656,719 + Pf1N1B4_1445 0.43 -1.1 1,656,719 + Pf1N1B4_1445 0.43 -0.3 1,656,720 - Pf1N1B4_1445 0.43 +0.4 1,656,720 - Pf1N1B4_1445 0.43 -0.2 1,656,720 - Pf1N1B4_1445 0.43 +0.6 1,656,720 - Pf1N1B4_1445 0.43 +0.0 1,656,735 + Pf1N1B4_1445 0.45 -2.2 1,656,735 + Pf1N1B4_1445 0.45 +1.2 1,656,735 + Pf1N1B4_1445 0.45 -0.2 1,656,736 - Pf1N1B4_1445 0.45 -0.2 1,656,736 - Pf1N1B4_1445 0.45 +1.7 1,656,736 - Pf1N1B4_1445 0.45 -0.4 1,656,736 - Pf1N1B4_1445 0.45 +1.0 1,656,736 - Pf1N1B4_1445 0.45 -1.4 1,656,779 + Pf1N1B4_1445 0.51 +0.7 1,656,780 - Pf1N1B4_1445 0.51 +0.8 1,656,780 - Pf1N1B4_1445 0.51 +0.7 1,656,780 - Pf1N1B4_1445 0.51 +0.6 1,656,780 - Pf1N1B4_1445 0.51 -0.5 1,656,830 + +0.6 1,656,830 + Pf1N1B4_1445 0.58 -1.2 1,656,830 + Pf1N1B4_1445 0.58 -0.4 1,656,830 + Pf1N1B4_1445 0.58 -0.5 1,656,830 + Pf1N1B4_1445 0.58 -1.4 1,656,830 + Pf1N1B4_1445 0.58 -0.4 1,656,830 + Pf1N1B4_1445 0.58 -0.8 1,656,830 + Pf1N1B4_1445 0.58 -1.5 1,656,831 - Pf1N1B4_1445 0.58 +1.3 1,656,831 - Pf1N1B4_1445 0.58 +0.0 1,656,831 - Pf1N1B4_1445 0.58 -0.2 1,656,831 - Pf1N1B4_1445 0.58 +0.1 1,656,831 - Pf1N1B4_1445 0.58 -0.4 1,656,831 - Pf1N1B4_1445 0.58 -0.0 1,656,831 - Pf1N1B4_1445 0.58 +0.8 1,656,847 + +0.2 1,656,847 + Pf1N1B4_1445 0.60 -0.4 1,656,847 + Pf1N1B4_1445 0.60 -1.0 1,656,848 - Pf1N1B4_1445 0.60 -0.4 1,656,848 - Pf1N1B4_1445 0.60 -0.4 1,656,848 - Pf1N1B4_1445 0.60 -0.6 1,656,848 - Pf1N1B4_1445 0.60 +0.2 1,656,848 - +0.2 1,656,848 - Pf1N1B4_1445 0.60 +0.0 1,657,014 - Pf1N1B4_1445 0.82 +2.0 1,657,034 + Pf1N1B4_1445 0.85 -0.8 1,657,034 + Pf1N1B4_1445 0.85 -0.1 1,657,035 - Pf1N1B4_1445 0.85 +1.8 1,657,035 - Pf1N1B4_1445 0.85 -0.7 1,657,035 - -0.1 1,657,139 - -1.6 1,657,237 + +1.0 1,657,238 - +0.5 1,657,238 - +0.8 1,657,238 - -0.2 1,657,238 - -1.6 1,657,238 - -0.2 1,657,296 + Pf1N1B4_1446 0.15 +0.6 1,657,378 - Pf1N1B4_1446 0.21 +0.1 1,657,630 + +0.2 1,657,630 + Pf1N1B4_1446 0.41 -0.2 1,657,868 + Pf1N1B4_1446 0.60 -1.6 1,657,869 - Pf1N1B4_1446 0.60 -1.9 1,657,869 - Pf1N1B4_1446 0.60 -0.9 1,657,869 - Pf1N1B4_1446 0.60 -1.6 1,657,869 - Pf1N1B4_1446 0.60 -2.0 1,657,869 - Pf1N1B4_1446 0.60 -1.4 1,657,893 - Pf1N1B4_1446 0.62 -1.6 1,658,383 + +1.2 1,658,383 + -0.9 1,658,383 + -0.0 1,658,384 - -1.5 1,658,384 - +1.2 1,658,384 - -0.4 1,658,384 - -1.1 1,658,384 - +0.9 1,658,412 + +0.1 1,658,412 + +0.6 1,658,413 - +0.2 1,658,413 - -0.1 1,658,413 - +0.2 1,658,413 - -0.4 1,658,413 - +0.4 1,658,565 - +0.8 1,658,565 - +0.7 1,658,765 + Pf1N1B4_1447 0.11 -1.5 1,658,766 - Pf1N1B4_1447 0.11 +1.2 1,658,766 - Pf1N1B4_1447 0.11 -1.4 1,658,909 + Pf1N1B4_1447 0.19 -1.6 1,658,909 + Pf1N1B4_1447 0.19 -0.4 1,658,910 - Pf1N1B4_1447 0.19 -0.4 1,658,910 - Pf1N1B4_1447 0.19 -1.4 1,658,910 - Pf1N1B4_1447 0.19 -1.2 1,658,910 - Pf1N1B4_1447 0.19 -1.8 1,658,910 - +0.2 1,658,910 - Pf1N1B4_1447 0.19 +1.6 1,658,910 - Pf1N1B4_1447 0.19 +0.4 1,658,975 + Pf1N1B4_1447 0.22 -0.2 1,658,975 + Pf1N1B4_1447 0.22 -0.5 1,658,976 - Pf1N1B4_1447 0.22 -0.8 1,659,080 + Pf1N1B4_1447 0.28 -2.2 1,659,081 - Pf1N1B4_1447 0.28 +0.2 1,659,081 - Pf1N1B4_1447 0.28 -0.2 1,659,175 + Pf1N1B4_1447 0.33 -0.4 1,659,175 + Pf1N1B4_1447 0.33 -0.8 1,659,175 + Pf1N1B4_1447 0.33 -0.5 1,659,175 + Pf1N1B4_1447 0.33 -1.9 1,659,175 + Pf1N1B4_1447 0.33 -2.2 1,659,176 - Pf1N1B4_1447 0.33 +0.1 1,659,176 - Pf1N1B4_1447 0.33 +2.8 1,659,176 - Pf1N1B4_1447 0.33 +0.4 1,659,176 - Pf1N1B4_1447 0.33 +0.2 1,659,176 - Pf1N1B4_1447 0.33 -1.5 1,659,176 - Pf1N1B4_1447 0.33 -0.4 1,659,176 - Pf1N1B4_1447 0.33 -0.6 1,659,302 + Pf1N1B4_1447 0.40 +0.0 1,659,302 + Pf1N1B4_1447 0.40 +0.2 1,659,342 - Pf1N1B4_1447 0.42 +0.9 1,659,357 + Pf1N1B4_1447 0.43 -1.1 1,659,357 + Pf1N1B4_1447 0.43 +0.9 1,659,358 - Pf1N1B4_1447 0.43 +1.0
Or see this region's nucleotide sequence