Experiment: Control soil assay; outgrowth in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS01115 and HSERO_RS01120 are separated by 115 nucleotides HSERO_RS01120 and HSERO_RS01125 are separated by 28 nucleotides HSERO_RS01125 and HSERO_RS01130 are separated by 81 nucleotides HSERO_RS01130 and HSERO_RS01135 overlap by 4 nucleotides
HSERO_RS01115: HSERO_RS01115 - integrase, at 233,271 to 235,010
_RS01115
HSERO_RS01120: HSERO_RS01120 - hypothetical protein, at 235,126 to 236,010
_RS01120
HSERO_RS01125: HSERO_RS01125 - bacteriophage transcription regulator protein, at 236,039 to 236,407
_RS01125
HSERO_RS01130: HSERO_RS01130 - hypothetical protein, at 236,489 to 236,929
_RS01130
HSERO_RS01135: HSERO_RS01135 - hypothetical protein, at 236,926 to 237,348
_RS01135
Position (kb) 235
236
237 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 234.204 kb on - strand, within HSERO_RS01115 at 234.624 kb on - strand, within HSERO_RS01115 at 234.627 kb on + strand, within HSERO_RS01115 at 234.635 kb on - strand, within HSERO_RS01115 at 234.905 kb on + strand at 235.095 kb on - strand at 235.153 kb on - strand at 235.237 kb on + strand, within HSERO_RS01120 at 235.240 kb on - strand, within HSERO_RS01120 at 235.388 kb on + strand, within HSERO_RS01120 at 235.433 kb on - strand at 235.541 kb on + strand, within HSERO_RS01120 at 235.860 kb on + strand, within HSERO_RS01120 at 235.860 kb on + strand, within HSERO_RS01120 at 235.945 kb on + strand at 235.945 kb on + strand at 236.033 kb on - strand at 236.138 kb on - strand, within HSERO_RS01125 at 236.213 kb on - strand, within HSERO_RS01125 at 236.301 kb on + strand at 236.327 kb on - strand, within HSERO_RS01125 at 236.404 kb on - strand at 236.410 kb on - strand at 236.540 kb on + strand, within HSERO_RS01130 at 236.600 kb on - strand, within HSERO_RS01130 at 236.633 kb on + strand, within HSERO_RS01130 at 236.633 kb on + strand, within HSERO_RS01130 at 236.695 kb on + strand, within HSERO_RS01130 at 236.811 kb on + strand, within HSERO_RS01130 at 236.819 kb on - strand at 236.828 kb on - strand, within HSERO_RS01130 at 236.990 kb on - strand, within HSERO_RS01135 at 236.990 kb on - strand, within HSERO_RS01135
Per-strain Table
Position Strand Gene LocusTag Fraction Control soil assay; outgrowth in LB remove 234,204 - HSERO_RS01115 0.54 -0.8 234,624 - HSERO_RS01115 0.78 -0.3 234,627 + HSERO_RS01115 0.78 +1.1 234,635 - HSERO_RS01115 0.78 -0.0 234,905 + +0.5 235,095 - -3.2 235,153 - -0.8 235,237 + HSERO_RS01120 0.13 -1.6 235,240 - HSERO_RS01120 0.13 -2.1 235,388 + HSERO_RS01120 0.30 -1.0 235,433 - +1.0 235,541 + HSERO_RS01120 0.47 -0.2 235,860 + HSERO_RS01120 0.83 -0.8 235,860 + HSERO_RS01120 0.83 -1.3 235,945 + -0.2 235,945 + +0.5 236,033 - -0.6 236,138 - HSERO_RS01125 0.27 +1.5 236,213 - HSERO_RS01125 0.47 -0.4 236,301 + +0.1 236,327 - HSERO_RS01125 0.78 +0.1 236,404 - +0.0 236,410 - -2.1 236,540 + HSERO_RS01130 0.12 +1.1 236,600 - HSERO_RS01130 0.25 -1.4 236,633 + HSERO_RS01130 0.33 -1.8 236,633 + HSERO_RS01130 0.33 -0.0 236,695 + HSERO_RS01130 0.47 -0.7 236,811 + HSERO_RS01130 0.73 -0.3 236,819 - -0.9 236,828 - HSERO_RS01130 0.77 +0.3 236,990 - HSERO_RS01135 0.15 -2.5 236,990 - HSERO_RS01135 0.15 -2.7
Or see this region's nucleotide sequence