Experiment: Recovery broth with Pyrimethamine 0.004 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS18155 and BBR_RS18160 are separated by 40 nucleotides BBR_RS18160 and BBR_RS18165 are separated by 94 nucleotides BBR_RS18165 and BBR_RS18170 are separated by 116 nucleotides
BBR_RS18155: BBR_RS18155 - acyl-CoA thioesterase II, at 1,858,899 to 1,859,804
_RS18155
BBR_RS18160: BBR_RS18160 - DUF3180 domain-containing protein, at 1,859,845 to 1,860,372
_RS18160
BBR_RS18165: BBR_RS18165 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine diphosphokinase, at 1,860,467 to 1,862,077
_RS18165
BBR_RS18170: BBR_RS18170 - dihydropteroate synthase, at 1,862,194 to 1,863,069
_RS18170
Position (kb)
1860
1861
1862
1863 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1859.480 kb on - strand, within BBR_RS18155 at 1859.517 kb on + strand, within BBR_RS18155 at 1859.517 kb on + strand, within BBR_RS18155 at 1859.517 kb on + strand, within BBR_RS18155 at 1859.517 kb on + strand, within BBR_RS18155 at 1859.525 kb on - strand, within BBR_RS18155 at 1859.525 kb on - strand, within BBR_RS18155 at 1859.525 kb on - strand, within BBR_RS18155 at 1859.535 kb on - strand, within BBR_RS18155 at 1859.565 kb on + strand, within BBR_RS18155 at 1859.571 kb on + strand, within BBR_RS18155 at 1859.571 kb on + strand, within BBR_RS18155 at 1859.571 kb on + strand, within BBR_RS18155 at 1859.631 kb on + strand, within BBR_RS18155 at 1859.682 kb on + strand, within BBR_RS18155 at 1859.682 kb on + strand, within BBR_RS18155 at 1859.690 kb on - strand, within BBR_RS18155 at 1859.690 kb on - strand, within BBR_RS18155 at 1859.710 kb on + strand, within BBR_RS18155 at 1859.718 kb on - strand at 1859.718 kb on - strand at 1859.720 kb on + strand at 1859.725 kb on + strand at 1859.728 kb on - strand at 1859.733 kb on + strand at 1859.778 kb on + strand at 1859.778 kb on + strand at 1859.778 kb on + strand at 1859.782 kb on - strand at 1859.786 kb on - strand at 1859.812 kb on + strand at 1859.843 kb on + strand at 1859.860 kb on - strand at 1859.865 kb on - strand at 1859.905 kb on + strand, within BBR_RS18160 at 1859.912 kb on + strand, within BBR_RS18160 at 1859.913 kb on - strand, within BBR_RS18160 at 1859.913 kb on - strand, within BBR_RS18160 at 1859.962 kb on + strand, within BBR_RS18160 at 1859.974 kb on + strand, within BBR_RS18160 at 1859.974 kb on + strand, within BBR_RS18160 at 1859.974 kb on + strand, within BBR_RS18160 at 1859.979 kb on + strand, within BBR_RS18160 at 1859.987 kb on - strand, within BBR_RS18160 at 1860.010 kb on - strand, within BBR_RS18160 at 1860.010 kb on - strand, within BBR_RS18160 at 1860.012 kb on + strand, within BBR_RS18160 at 1860.014 kb on + strand, within BBR_RS18160 at 1860.014 kb on + strand, within BBR_RS18160 at 1860.017 kb on - strand, within BBR_RS18160 at 1860.022 kb on - strand, within BBR_RS18160 at 1860.022 kb on - strand, within BBR_RS18160 at 1860.036 kb on + strand, within BBR_RS18160 at 1860.118 kb on + strand, within BBR_RS18160 at 1860.135 kb on - strand, within BBR_RS18160 at 1860.184 kb on + strand, within BBR_RS18160 at 1860.200 kb on + strand, within BBR_RS18160 at 1860.200 kb on + strand, within BBR_RS18160 at 1860.200 kb on + strand, within BBR_RS18160 at 1860.200 kb on + strand, within BBR_RS18160 at 1860.201 kb on - strand, within BBR_RS18160 at 1860.210 kb on + strand, within BBR_RS18160 at 1860.215 kb on - strand, within BBR_RS18160 at 1860.220 kb on + strand, within BBR_RS18160 at 1860.223 kb on - strand, within BBR_RS18160 at 1860.228 kb on - strand, within BBR_RS18160 at 1860.300 kb on + strand, within BBR_RS18160 at 1860.302 kb on - strand, within BBR_RS18160 at 1860.324 kb on + strand at 1860.324 kb on + strand at 1860.334 kb on + strand at 1860.439 kb on + strand at 1860.499 kb on - strand at 1860.535 kb on + strand at 1860.584 kb on - strand at 1860.657 kb on - strand, within BBR_RS18165 at 1860.657 kb on - strand, within BBR_RS18165 at 1860.663 kb on + strand, within BBR_RS18165 at 1860.665 kb on - strand, within BBR_RS18165 at 1860.671 kb on - strand, within BBR_RS18165 at 1860.673 kb on - strand, within BBR_RS18165 at 1860.744 kb on - strand, within BBR_RS18165 at 1860.756 kb on + strand, within BBR_RS18165 at 1860.793 kb on - strand, within BBR_RS18165 at 1860.828 kb on + strand, within BBR_RS18165 at 1860.836 kb on - strand, within BBR_RS18165 at 1860.836 kb on - strand, within BBR_RS18165 at 1860.865 kb on + strand, within BBR_RS18165 at 1860.966 kb on - strand, within BBR_RS18165 at 1861.004 kb on + strand, within BBR_RS18165 at 1861.012 kb on - strand, within BBR_RS18165 at 1861.034 kb on + strand, within BBR_RS18165 at 1861.057 kb on - strand, within BBR_RS18165 at 1861.075 kb on - strand, within BBR_RS18165 at 1861.080 kb on - strand, within BBR_RS18165 at 1861.098 kb on - strand, within BBR_RS18165 at 1861.098 kb on - strand, within BBR_RS18165 at 1861.185 kb on + strand, within BBR_RS18165 at 1861.244 kb on + strand, within BBR_RS18165 at 1862.125 kb on - strand at 1862.150 kb on - strand at 1862.150 kb on - strand at 1862.247 kb on - strand at 1862.333 kb on - strand, within BBR_RS18170 at 1862.371 kb on - strand, within BBR_RS18170 at 1862.371 kb on - strand, within BBR_RS18170 at 1862.514 kb on + strand, within BBR_RS18170 at 1862.810 kb on - strand, within BBR_RS18170 at 1862.970 kb on - strand, within BBR_RS18170
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Pyrimethamine 0.004 mM remove 1,859,480 - BBR_RS18155 0.64 +0.1 1,859,517 + BBR_RS18155 0.68 -0.0 1,859,517 + BBR_RS18155 0.68 +0.5 1,859,517 + BBR_RS18155 0.68 -0.5 1,859,517 + BBR_RS18155 0.68 -0.1 1,859,525 - BBR_RS18155 0.69 -0.7 1,859,525 - BBR_RS18155 0.69 +0.3 1,859,525 - BBR_RS18155 0.69 -0.6 1,859,535 - BBR_RS18155 0.70 +0.5 1,859,565 + BBR_RS18155 0.74 -0.7 1,859,571 + BBR_RS18155 0.74 -1.2 1,859,571 + BBR_RS18155 0.74 -0.9 1,859,571 + BBR_RS18155 0.74 -0.8 1,859,631 + BBR_RS18155 0.81 +0.2 1,859,682 + BBR_RS18155 0.86 -0.7 1,859,682 + BBR_RS18155 0.86 +0.3 1,859,690 - BBR_RS18155 0.87 +0.8 1,859,690 - BBR_RS18155 0.87 +0.8 1,859,710 + BBR_RS18155 0.90 +0.1 1,859,718 - -0.0 1,859,718 - -2.8 1,859,720 + -0.8 1,859,725 + -0.5 1,859,728 - +0.3 1,859,733 + +2.7 1,859,778 + +0.0 1,859,778 + -0.2 1,859,778 + +0.2 1,859,782 - -0.3 1,859,786 - -0.9 1,859,812 + +0.0 1,859,843 + -0.0 1,859,860 - +0.1 1,859,865 - -0.4 1,859,905 + BBR_RS18160 0.11 +0.4 1,859,912 + BBR_RS18160 0.13 -1.5 1,859,913 - BBR_RS18160 0.13 +0.3 1,859,913 - BBR_RS18160 0.13 +0.7 1,859,962 + BBR_RS18160 0.22 -0.4 1,859,974 + BBR_RS18160 0.24 +0.1 1,859,974 + BBR_RS18160 0.24 +0.2 1,859,974 + BBR_RS18160 0.24 -0.0 1,859,979 + BBR_RS18160 0.25 +0.2 1,859,987 - BBR_RS18160 0.27 +0.5 1,860,010 - BBR_RS18160 0.31 -0.1 1,860,010 - BBR_RS18160 0.31 -0.2 1,860,012 + BBR_RS18160 0.32 +0.0 1,860,014 + BBR_RS18160 0.32 +0.1 1,860,014 + BBR_RS18160 0.32 -0.6 1,860,017 - BBR_RS18160 0.33 -0.1 1,860,022 - BBR_RS18160 0.34 +0.4 1,860,022 - BBR_RS18160 0.34 +0.8 1,860,036 + BBR_RS18160 0.36 -0.8 1,860,118 + BBR_RS18160 0.52 -1.3 1,860,135 - BBR_RS18160 0.55 -0.2 1,860,184 + BBR_RS18160 0.64 -0.1 1,860,200 + BBR_RS18160 0.67 -0.7 1,860,200 + BBR_RS18160 0.67 -0.9 1,860,200 + BBR_RS18160 0.67 +0.3 1,860,200 + BBR_RS18160 0.67 +0.6 1,860,201 - BBR_RS18160 0.67 -0.2 1,860,210 + BBR_RS18160 0.69 +0.5 1,860,215 - BBR_RS18160 0.70 +0.0 1,860,220 + BBR_RS18160 0.71 -0.7 1,860,223 - BBR_RS18160 0.72 +0.1 1,860,228 - BBR_RS18160 0.73 -0.6 1,860,300 + BBR_RS18160 0.86 +0.1 1,860,302 - BBR_RS18160 0.87 -1.6 1,860,324 + -0.3 1,860,324 + +0.3 1,860,334 + -1.8 1,860,439 + +0.6 1,860,499 - -1.9 1,860,535 + -0.5 1,860,584 - -1.9 1,860,657 - BBR_RS18165 0.12 -0.3 1,860,657 - BBR_RS18165 0.12 -0.8 1,860,663 + BBR_RS18165 0.12 -0.5 1,860,665 - BBR_RS18165 0.12 -0.7 1,860,671 - BBR_RS18165 0.13 -0.7 1,860,673 - BBR_RS18165 0.13 -0.8 1,860,744 - BBR_RS18165 0.17 -0.3 1,860,756 + BBR_RS18165 0.18 +0.2 1,860,793 - BBR_RS18165 0.20 -2.7 1,860,828 + BBR_RS18165 0.22 -1.3 1,860,836 - BBR_RS18165 0.23 -0.3 1,860,836 - BBR_RS18165 0.23 -1.5 1,860,865 + BBR_RS18165 0.25 -1.5 1,860,966 - BBR_RS18165 0.31 -0.7 1,861,004 + BBR_RS18165 0.33 -0.6 1,861,012 - BBR_RS18165 0.34 -1.1 1,861,034 + BBR_RS18165 0.35 -0.7 1,861,057 - BBR_RS18165 0.37 -0.4 1,861,075 - BBR_RS18165 0.38 -0.7 1,861,080 - BBR_RS18165 0.38 -0.0 1,861,098 - BBR_RS18165 0.39 +0.2 1,861,098 - BBR_RS18165 0.39 -0.2 1,861,185 + BBR_RS18165 0.45 -0.8 1,861,244 + BBR_RS18165 0.48 +0.8 1,862,125 - +0.2 1,862,150 - +0.2 1,862,150 - +0.4 1,862,247 - -2.0 1,862,333 - BBR_RS18170 0.16 -2.6 1,862,371 - BBR_RS18170 0.20 -1.7 1,862,371 - BBR_RS18170 0.20 -2.7 1,862,514 + BBR_RS18170 0.37 -1.1 1,862,810 - BBR_RS18170 0.70 -1.0 1,862,970 - BBR_RS18170 0.89 -0.2
Or see this region's nucleotide sequence