Experiment: nitrite 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_680 and Pf1N1B4_681 overlap by 8 nucleotides Pf1N1B4_681 and Pf1N1B4_682 are separated by 361 nucleotides Pf1N1B4_682 and Pf1N1B4_683 are separated by 42 nucleotides
Pf1N1B4_680 - 2-polyprenylphenol hydroxylase and related flavodoxin oxidoreductases, at 777,151 to 778,086
_680
Pf1N1B4_681 - FOG: GGDEF domain, at 778,079 to 779,185
_681
Pf1N1B4_682 - hypothetical protein, at 779,547 to 779,699
_682
Pf1N1B4_683 - Fumarate hydratase class I, aerobic (EC 4.2.1.2), at 779,742 to 781,265
_683
Position (kb) 778
779
780 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 777.163 kb on + strand at 777.209 kb on + strand at 777.210 kb on - strand at 777.210 kb on - strand at 777.383 kb on + strand, within Pf1N1B4_680 at 777.383 kb on + strand, within Pf1N1B4_680 at 777.384 kb on - strand, within Pf1N1B4_680 at 777.534 kb on - strand, within Pf1N1B4_680 at 777.680 kb on + strand, within Pf1N1B4_680 at 777.761 kb on - strand, within Pf1N1B4_680 at 777.990 kb on + strand at 777.991 kb on - strand, within Pf1N1B4_680 at 777.991 kb on - strand, within Pf1N1B4_680 at 777.991 kb on - strand, within Pf1N1B4_680 at 777.991 kb on - strand, within Pf1N1B4_680 at 777.993 kb on - strand at 778.266 kb on - strand, within Pf1N1B4_681 at 778.651 kb on + strand, within Pf1N1B4_681 at 778.712 kb on + strand, within Pf1N1B4_681 at 778.712 kb on + strand at 778.713 kb on - strand, within Pf1N1B4_681 at 778.713 kb on - strand, within Pf1N1B4_681 at 778.873 kb on - strand, within Pf1N1B4_681 at 778.873 kb on - strand, within Pf1N1B4_681 at 778.947 kb on - strand, within Pf1N1B4_681 at 778.947 kb on - strand, within Pf1N1B4_681 at 779.076 kb on + strand at 779.077 kb on - strand at 779.125 kb on - strand at 779.125 kb on - strand at 779.125 kb on - strand at 779.226 kb on + strand at 779.226 kb on + strand at 779.226 kb on + strand at 779.227 kb on - strand at 779.227 kb on - strand at 779.231 kb on + strand at 779.232 kb on - strand at 779.232 kb on - strand at 779.265 kb on + strand at 779.266 kb on - strand at 779.328 kb on - strand at 779.407 kb on - strand at 779.407 kb on - strand at 779.407 kb on - strand at 779.407 kb on - strand at 779.474 kb on + strand at 779.474 kb on + strand at 779.474 kb on + strand at 779.474 kb on + strand at 779.474 kb on + strand at 779.474 kb on + strand at 779.474 kb on + strand at 779.474 kb on + strand at 779.474 kb on + strand at 779.475 kb on - strand at 779.475 kb on - strand at 779.475 kb on - strand at 779.475 kb on - strand at 779.475 kb on - strand at 779.475 kb on - strand at 779.475 kb on - strand at 779.544 kb on + strand at 779.544 kb on + strand at 779.545 kb on - strand at 779.545 kb on - strand at 779.612 kb on + strand, within Pf1N1B4_682 at 779.613 kb on - strand, within Pf1N1B4_682 at 779.707 kb on - strand at 779.806 kb on - strand at 779.808 kb on + strand at 779.848 kb on - strand at 779.848 kb on - strand at 779.848 kb on - strand at 780.049 kb on - strand, within Pf1N1B4_683 at 780.169 kb on + strand, within Pf1N1B4_683
Per-strain Table
Position Strand Gene LocusTag Fraction nitrite 10 mM remove 777,163 + -0.5 777,209 + +0.8 777,210 - +0.3 777,210 - -0.7 777,383 + Pf1N1B4_680 0.25 +0.7 777,383 + Pf1N1B4_680 0.25 +0.0 777,384 - Pf1N1B4_680 0.25 +0.7 777,534 - Pf1N1B4_680 0.41 -0.2 777,680 + Pf1N1B4_680 0.57 +0.1 777,761 - Pf1N1B4_680 0.65 +0.4 777,990 + +1.8 777,991 - Pf1N1B4_680 0.90 +0.1 777,991 - Pf1N1B4_680 0.90 -2.5 777,991 - Pf1N1B4_680 0.90 +0.1 777,991 - Pf1N1B4_680 0.90 -0.5 777,993 - -1.2 778,266 - Pf1N1B4_681 0.17 -1.5 778,651 + Pf1N1B4_681 0.52 +0.4 778,712 + Pf1N1B4_681 0.57 -1.5 778,712 + -1.5 778,713 - Pf1N1B4_681 0.57 -0.5 778,713 - Pf1N1B4_681 0.57 +0.3 778,873 - Pf1N1B4_681 0.72 -0.2 778,873 - Pf1N1B4_681 0.72 -1.2 778,947 - Pf1N1B4_681 0.78 -0.9 778,947 - Pf1N1B4_681 0.78 -0.3 779,076 + -2.6 779,077 - -0.4 779,125 - +1.1 779,125 - +0.1 779,125 - +1.8 779,226 + +0.7 779,226 + -0.7 779,226 + -0.1 779,227 - -1.2 779,227 - +1.8 779,231 + -2.2 779,232 - -0.7 779,232 - -1.2 779,265 + -0.0 779,266 - -0.9 779,328 - -1.0 779,407 - +0.4 779,407 - +1.1 779,407 - +2.6 779,407 - -0.5 779,474 + +0.0 779,474 + +0.6 779,474 + +0.6 779,474 + -1.3 779,474 + +1.2 779,474 + +0.6 779,474 + +0.8 779,474 + +0.8 779,474 + +1.6 779,475 - +0.8 779,475 - -0.3 779,475 - -0.2 779,475 - +0.8 779,475 - -0.0 779,475 - +2.2 779,475 - -0.2 779,544 + -1.3 779,544 + -0.5 779,545 - -0.6 779,545 - -1.2 779,612 + Pf1N1B4_682 0.42 +0.6 779,613 - Pf1N1B4_682 0.43 -0.6 779,707 - -1.2 779,806 - -2.0 779,808 + -0.7 779,848 - -2.7 779,848 - -1.9 779,848 - -1.2 780,049 - Pf1N1B4_683 0.20 -4.1 780,169 + Pf1N1B4_683 0.28 -2.9
Or see this region's nucleotide sequence