Experiment: nitrite 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_581 and Pf1N1B4_582 are separated by 157 nucleotides Pf1N1B4_582 and Pf1N1B4_583 are separated by 201 nucleotides Pf1N1B4_583 and Pf1N1B4_584 are separated by 25 nucleotides
Pf1N1B4_581 - membrane protein, putative, at 672,568 to 674,556
_581
Pf1N1B4_582 - FIG00959696: hypothetical protein, at 674,714 to 675,181
_582
Pf1N1B4_583 - FIG00957748: hypothetical protein, at 675,383 to 675,622
_583
Pf1N1B4_584 - Isopenicillin N epimerase (EC 5.1.1.17), at 675,648 to 676,829
_584
Position (kb) 675
676 Strain fitness (log2 ratio) -2
-1
0
1
2 at 674.410 kb on - strand at 674.558 kb on + strand at 674.559 kb on - strand at 674.559 kb on - strand at 674.559 kb on - strand at 674.559 kb on - strand at 674.559 kb on - strand at 674.634 kb on + strand at 674.656 kb on - strand at 674.715 kb on + strand at 674.715 kb on + strand at 674.715 kb on + strand at 674.715 kb on + strand at 674.715 kb on + strand at 674.716 kb on - strand at 674.716 kb on - strand at 674.716 kb on - strand at 674.716 kb on - strand at 674.716 kb on - strand at 674.716 kb on - strand at 674.716 kb on - strand at 674.716 kb on - strand at 674.716 kb on - strand at 674.781 kb on + strand, within Pf1N1B4_582 at 674.850 kb on + strand, within Pf1N1B4_582 at 674.851 kb on - strand, within Pf1N1B4_582 at 674.851 kb on - strand, within Pf1N1B4_582 at 674.851 kb on - strand, within Pf1N1B4_582 at 674.851 kb on - strand, within Pf1N1B4_582 at 674.851 kb on - strand, within Pf1N1B4_582 at 674.851 kb on - strand, within Pf1N1B4_582 at 675.056 kb on - strand, within Pf1N1B4_582 at 675.056 kb on - strand, within Pf1N1B4_582 at 675.056 kb on - strand, within Pf1N1B4_582 at 675.149 kb on + strand at 675.150 kb on - strand at 675.150 kb on - strand at 675.166 kb on + strand at 675.167 kb on - strand at 675.167 kb on - strand at 675.169 kb on + strand at 675.215 kb on + strand at 675.215 kb on + strand at 675.215 kb on + strand at 675.216 kb on - strand at 675.216 kb on - strand at 675.220 kb on - strand at 675.441 kb on - strand, within Pf1N1B4_583 at 675.441 kb on - strand, within Pf1N1B4_583 at 675.441 kb on - strand, within Pf1N1B4_583 at 675.441 kb on - strand, within Pf1N1B4_583 at 675.784 kb on - strand, within Pf1N1B4_584 at 675.960 kb on + strand, within Pf1N1B4_584 at 675.960 kb on + strand, within Pf1N1B4_584 at 675.961 kb on - strand, within Pf1N1B4_584 at 675.961 kb on - strand, within Pf1N1B4_584 at 675.961 kb on - strand, within Pf1N1B4_584 at 675.961 kb on - strand, within Pf1N1B4_584 at 675.961 kb on - strand at 675.995 kb on - strand at 676.008 kb on + strand, within Pf1N1B4_584 at 676.365 kb on + strand, within Pf1N1B4_584 at 676.365 kb on + strand, within Pf1N1B4_584 at 676.365 kb on + strand, within Pf1N1B4_584 at 676.366 kb on - strand, within Pf1N1B4_584 at 676.366 kb on - strand, within Pf1N1B4_584 at 676.366 kb on - strand, within Pf1N1B4_584 at 676.551 kb on + strand, within Pf1N1B4_584 at 676.551 kb on + strand, within Pf1N1B4_584 at 676.552 kb on - strand, within Pf1N1B4_584
Per-strain Table
Position Strand Gene LocusTag Fraction nitrite 10 mM remove 674,410 - -0.3 674,558 + -1.2 674,559 - -1.2 674,559 - -0.0 674,559 - +0.1 674,559 - +0.1 674,559 - +1.4 674,634 + +0.1 674,656 - +0.3 674,715 + -0.1 674,715 + -0.2 674,715 + +0.5 674,715 + +0.4 674,715 + -1.2 674,716 - -0.1 674,716 - -1.2 674,716 - +0.0 674,716 - +0.0 674,716 - -1.5 674,716 - +0.3 674,716 - -1.5 674,716 - +0.7 674,716 - +0.4 674,781 + Pf1N1B4_582 0.14 -1.7 674,850 + Pf1N1B4_582 0.29 +0.8 674,851 - Pf1N1B4_582 0.29 +0.4 674,851 - Pf1N1B4_582 0.29 +1.7 674,851 - Pf1N1B4_582 0.29 -2.3 674,851 - Pf1N1B4_582 0.29 +1.3 674,851 - Pf1N1B4_582 0.29 +1.8 674,851 - Pf1N1B4_582 0.29 -0.2 675,056 - Pf1N1B4_582 0.73 +0.4 675,056 - Pf1N1B4_582 0.73 +1.0 675,056 - Pf1N1B4_582 0.73 +0.8 675,149 + +2.4 675,150 - +0.0 675,150 - +0.8 675,166 + -0.2 675,167 - -1.7 675,167 - +1.8 675,169 + -0.4 675,215 + +0.8 675,215 + +0.2 675,215 + -0.2 675,216 - -0.6 675,216 - +0.1 675,220 - +2.6 675,441 - Pf1N1B4_583 0.24 -1.6 675,441 - Pf1N1B4_583 0.24 -1.1 675,441 - Pf1N1B4_583 0.24 -0.2 675,441 - Pf1N1B4_583 0.24 +0.8 675,784 - Pf1N1B4_584 0.12 +0.2 675,960 + Pf1N1B4_584 0.26 +0.6 675,960 + Pf1N1B4_584 0.26 +0.8 675,961 - Pf1N1B4_584 0.26 -0.3 675,961 - Pf1N1B4_584 0.26 +0.4 675,961 - Pf1N1B4_584 0.26 +0.8 675,961 - Pf1N1B4_584 0.26 -1.0 675,961 - +1.8 675,995 - +1.4 676,008 + Pf1N1B4_584 0.30 +0.3 676,365 + Pf1N1B4_584 0.61 -0.4 676,365 + Pf1N1B4_584 0.61 +0.5 676,365 + Pf1N1B4_584 0.61 -0.0 676,366 - Pf1N1B4_584 0.61 +2.2 676,366 - Pf1N1B4_584 0.61 -0.2 676,366 - Pf1N1B4_584 0.61 +0.4 676,551 + Pf1N1B4_584 0.76 +1.5 676,551 + Pf1N1B4_584 0.76 +0.1 676,552 - Pf1N1B4_584 0.76 +0.1
Or see this region's nucleotide sequence