Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5032

Experiment: nitrite 10 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5031 and Pf1N1B4_5032 are separated by 75 nucleotidesPf1N1B4_5032 and Pf1N1B4_5033 are separated by 69 nucleotidesPf1N1B4_5033 and Pf1N1B4_5034 are separated by 131 nucleotidesPf1N1B4_5034 and Pf1N1B4_5035 are separated by 306 nucleotides Pf1N1B4_5031 - Molybdenum transport ATP-binding protein ModC (TC 3.A.1.8.1), at 5,576,918 to 5,577,997 _5031 Pf1N1B4_5032 - Hypothetical protein PA2244 (similar to DNA topoisomerase IB, but possibly involved in glycosyl-transfer), at 5,578,073 to 5,579,110 _5032 Pf1N1B4_5033 - hypothetical protein, at 5,579,180 to 5,579,437 _5033 Pf1N1B4_5034 - hypothetical protein, at 5,579,569 to 5,579,799 _5034 Pf1N1B4_5035 - hypothetical protein, at 5,580,106 to 5,580,390 _5035 Position (kb) 5578 5579 5580Strain fitness (log2 ratio) -2 -1 0 1 2at 5577.375 kb on + strand, within Pf1N1B4_5031at 5577.376 kb on - strand, within Pf1N1B4_5031at 5577.376 kb on - strand, within Pf1N1B4_5031at 5577.376 kb on - strand, within Pf1N1B4_5031at 5577.462 kb on + strand, within Pf1N1B4_5031at 5577.462 kb on + strandat 5577.462 kb on + strand, within Pf1N1B4_5031at 5577.462 kb on + strand, within Pf1N1B4_5031at 5577.463 kb on - strand, within Pf1N1B4_5031at 5577.650 kb on + strand, within Pf1N1B4_5031at 5577.868 kb on - strand, within Pf1N1B4_5031at 5578.037 kb on + strandat 5578.119 kb on - strandat 5578.376 kb on + strand, within Pf1N1B4_5032at 5578.377 kb on - strand, within Pf1N1B4_5032at 5578.487 kb on - strand, within Pf1N1B4_5032at 5578.487 kb on - strand, within Pf1N1B4_5032at 5578.530 kb on - strand, within Pf1N1B4_5032at 5578.772 kb on + strand, within Pf1N1B4_5032at 5578.776 kb on + strand, within Pf1N1B4_5032at 5578.777 kb on - strandat 5578.964 kb on + strand, within Pf1N1B4_5032at 5578.965 kb on - strandat 5578.965 kb on - strand, within Pf1N1B4_5032at 5579.035 kb on + strandat 5579.036 kb on - strandat 5579.036 kb on - strandat 5579.036 kb on - strandat 5579.130 kb on - strandat 5579.179 kb on + strandat 5579.179 kb on + strandat 5579.179 kb on + strandat 5579.180 kb on - strandat 5579.194 kb on + strandat 5579.194 kb on + strandat 5579.194 kb on + strandat 5579.194 kb on + strandat 5579.200 kb on + strandat 5579.200 kb on + strandat 5579.200 kb on + strandat 5579.200 kb on + strandat 5579.201 kb on - strandat 5579.201 kb on - strandat 5579.564 kb on - strandat 5579.772 kb on - strand, within Pf1N1B4_5034at 5579.875 kb on + strandat 5579.876 kb on - strandat 5579.876 kb on - strandat 5580.044 kb on + strandat 5580.044 kb on + strandat 5580.044 kb on + strandat 5580.045 kb on - strandat 5580.045 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrite 10 mM
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5,577,375 + Pf1N1B4_5031 0.42 +0.3
5,577,376 - Pf1N1B4_5031 0.42 -1.8
5,577,376 - Pf1N1B4_5031 0.42 +0.6
5,577,376 - Pf1N1B4_5031 0.42 +0.6
5,577,462 + Pf1N1B4_5031 0.50 -0.4
5,577,462 + -0.0
5,577,462 + Pf1N1B4_5031 0.50 -1.6
5,577,462 + Pf1N1B4_5031 0.50 -0.4
5,577,463 - Pf1N1B4_5031 0.50 -0.9
5,577,650 + Pf1N1B4_5031 0.68 +0.6
5,577,868 - Pf1N1B4_5031 0.88 +0.3
5,578,037 + -0.4
5,578,119 - -0.8
5,578,376 + Pf1N1B4_5032 0.29 +0.6
5,578,377 - Pf1N1B4_5032 0.29 -0.0
5,578,487 - Pf1N1B4_5032 0.40 -0.1
5,578,487 - Pf1N1B4_5032 0.40 -0.1
5,578,530 - Pf1N1B4_5032 0.44 -0.8
5,578,772 + Pf1N1B4_5032 0.67 +0.6
5,578,776 + Pf1N1B4_5032 0.68 +0.6
5,578,777 - -1.8
5,578,964 + Pf1N1B4_5032 0.86 +0.6
5,578,965 - +0.6
5,578,965 - Pf1N1B4_5032 0.86 +0.1
5,579,035 + -1.4
5,579,036 - +2.4
5,579,036 - +1.0
5,579,036 - +0.1
5,579,130 - +2.1
5,579,179 + -0.4
5,579,179 + +1.3
5,579,179 + +0.7
5,579,180 - -1.8
5,579,194 + -0.7
5,579,194 + -1.0
5,579,194 + +0.2
5,579,194 + +0.0
5,579,200 + +1.3
5,579,200 + +0.1
5,579,200 + +1.6
5,579,200 + +0.6
5,579,201 - -1.2
5,579,201 - +1.0
5,579,564 - +0.6
5,579,772 - Pf1N1B4_5034 0.88 +0.7
5,579,875 + -0.0
5,579,876 - -1.9
5,579,876 - +0.1
5,580,044 + +0.6
5,580,044 + -0.0
5,580,044 + +0.9
5,580,045 - +1.3
5,580,045 - -0.0

Or see this region's nucleotide sequence