Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_501

Experiment: nitrite 10 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_500 and Pf1N1B4_501 are separated by 75 nucleotidesPf1N1B4_501 and Pf1N1B4_502 are separated by 57 nucleotides Pf1N1B4_500 - Von Willebrand factor type A domain protein, associated with Flp pilus assembly, at 591,940 to 593,286 _500 Pf1N1B4_501 - Flp pilus assembly protein RcpC/CpaB, at 593,362 to 594,132 _501 Pf1N1B4_502 - Predicted ATPase with chaperone activity, associated with Flp pilus assembly, at 594,190 to 595,524 _502 Position (kb) 593 594 595Strain fitness (log2 ratio) -2 -1 0 1 2 3at 592.368 kb on + strand, within Pf1N1B4_500at 592.382 kb on + strand, within Pf1N1B4_500at 592.382 kb on + strand, within Pf1N1B4_500at 592.383 kb on - strand, within Pf1N1B4_500at 592.383 kb on - strand, within Pf1N1B4_500at 592.383 kb on - strand, within Pf1N1B4_500at 592.455 kb on - strand, within Pf1N1B4_500at 592.455 kb on - strand, within Pf1N1B4_500at 592.524 kb on + strandat 592.657 kb on + strand, within Pf1N1B4_500at 592.657 kb on + strand, within Pf1N1B4_500at 592.657 kb on + strand, within Pf1N1B4_500at 592.861 kb on - strand, within Pf1N1B4_500at 592.861 kb on - strand, within Pf1N1B4_500at 592.861 kb on - strand, within Pf1N1B4_500at 592.862 kb on + strand, within Pf1N1B4_500at 592.862 kb on + strand, within Pf1N1B4_500at 592.862 kb on + strand, within Pf1N1B4_500at 592.863 kb on - strand, within Pf1N1B4_500at 592.863 kb on - strand, within Pf1N1B4_500at 592.863 kb on - strand, within Pf1N1B4_500at 592.863 kb on - strand, within Pf1N1B4_500at 592.863 kb on - strand, within Pf1N1B4_500at 592.886 kb on + strand, within Pf1N1B4_500at 592.887 kb on - strand, within Pf1N1B4_500at 592.887 kb on - strand, within Pf1N1B4_500at 592.887 kb on - strand, within Pf1N1B4_500at 592.945 kb on + strand, within Pf1N1B4_500at 593.028 kb on + strand, within Pf1N1B4_500at 593.029 kb on - strand, within Pf1N1B4_500at 593.029 kb on - strand, within Pf1N1B4_500at 593.188 kb on + strandat 593.218 kb on + strandat 593.219 kb on - strandat 593.219 kb on - strandat 593.300 kb on - strandat 593.331 kb on - strandat 593.374 kb on + strandat 593.375 kb on - strandat 593.406 kb on + strandat 593.406 kb on + strandat 593.623 kb on + strand, within Pf1N1B4_501at 593.624 kb on - strand, within Pf1N1B4_501at 593.624 kb on - strand, within Pf1N1B4_501at 593.624 kb on - strand, within Pf1N1B4_501at 593.624 kb on - strand, within Pf1N1B4_501at 593.986 kb on + strand, within Pf1N1B4_501at 594.162 kb on + strandat 594.163 kb on - strandat 594.180 kb on + strandat 594.180 kb on + strandat 594.215 kb on + strandat 594.215 kb on + strandat 594.215 kb on + strandat 594.215 kb on + strandat 594.215 kb on + strandat 594.215 kb on + strandat 594.216 kb on - strandat 594.216 kb on - strandat 594.348 kb on - strand, within Pf1N1B4_502at 594.348 kb on - strand, within Pf1N1B4_502at 594.361 kb on + strand, within Pf1N1B4_502at 594.361 kb on + strand, within Pf1N1B4_502at 594.361 kb on + strandat 594.361 kb on + strand, within Pf1N1B4_502at 594.361 kb on + strand, within Pf1N1B4_502at 594.361 kb on + strand, within Pf1N1B4_502at 594.361 kb on + strandat 594.361 kb on + strand, within Pf1N1B4_502at 594.362 kb on - strand, within Pf1N1B4_502at 594.362 kb on - strand, within Pf1N1B4_502at 594.362 kb on - strand, within Pf1N1B4_502at 594.362 kb on - strand, within Pf1N1B4_502at 594.365 kb on + strand, within Pf1N1B4_502at 594.366 kb on - strand, within Pf1N1B4_502at 594.431 kb on + strand, within Pf1N1B4_502at 594.432 kb on - strand, within Pf1N1B4_502at 594.607 kb on + strand, within Pf1N1B4_502at 594.881 kb on + strand, within Pf1N1B4_502at 594.881 kb on + strand, within Pf1N1B4_502at 594.881 kb on + strand, within Pf1N1B4_502at 594.881 kb on + strand, within Pf1N1B4_502at 594.881 kb on + strand, within Pf1N1B4_502at 594.882 kb on - strand, within Pf1N1B4_502at 594.882 kb on - strand, within Pf1N1B4_502at 594.968 kb on + strand, within Pf1N1B4_502at 594.968 kb on + strand, within Pf1N1B4_502at 594.968 kb on + strand, within Pf1N1B4_502at 594.969 kb on - strand, within Pf1N1B4_502at 594.969 kb on - strand, within Pf1N1B4_502at 594.969 kb on - strand, within Pf1N1B4_502at 594.969 kb on - strand, within Pf1N1B4_502at 594.995 kb on + strand, within Pf1N1B4_502at 594.996 kb on - strand, within Pf1N1B4_502at 594.996 kb on - strand, within Pf1N1B4_502at 594.996 kb on - strand, within Pf1N1B4_502at 595.060 kb on + strand, within Pf1N1B4_502

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrite 10 mM
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592,368 + Pf1N1B4_500 0.32 +0.7
592,382 + Pf1N1B4_500 0.33 +0.0
592,382 + Pf1N1B4_500 0.33 +0.8
592,383 - Pf1N1B4_500 0.33 +1.8
592,383 - Pf1N1B4_500 0.33 +0.0
592,383 - Pf1N1B4_500 0.33 +0.0
592,455 - Pf1N1B4_500 0.38 +0.8
592,455 - Pf1N1B4_500 0.38 +1.1
592,524 + -0.2
592,657 + Pf1N1B4_500 0.53 -0.2
592,657 + Pf1N1B4_500 0.53 -0.4
592,657 + Pf1N1B4_500 0.53 +0.0
592,861 - Pf1N1B4_500 0.68 +0.6
592,861 - Pf1N1B4_500 0.68 +0.1
592,861 - Pf1N1B4_500 0.68 -1.4
592,862 + Pf1N1B4_500 0.68 -0.4
592,862 + Pf1N1B4_500 0.68 +0.4
592,862 + Pf1N1B4_500 0.68 +0.8
592,863 - Pf1N1B4_500 0.69 -0.5
592,863 - Pf1N1B4_500 0.69 -0.4
592,863 - Pf1N1B4_500 0.69 +0.1
592,863 - Pf1N1B4_500 0.69 +0.3
592,863 - Pf1N1B4_500 0.69 +1.0
592,886 + Pf1N1B4_500 0.70 +0.2
592,887 - Pf1N1B4_500 0.70 +0.4
592,887 - Pf1N1B4_500 0.70 +0.8
592,887 - Pf1N1B4_500 0.70 +3.6
592,945 + Pf1N1B4_500 0.75 -0.7
593,028 + Pf1N1B4_500 0.81 +0.2
593,029 - Pf1N1B4_500 0.81 +1.8
593,029 - Pf1N1B4_500 0.81 +0.1
593,188 + -0.4
593,218 + +0.3
593,219 - -0.4
593,219 - -1.3
593,300 - +1.8
593,331 - +1.0
593,374 + +1.5
593,375 - -0.4
593,406 + -0.0
593,406 + -0.5
593,623 + Pf1N1B4_501 0.34 -0.6
593,624 - Pf1N1B4_501 0.34 -0.2
593,624 - Pf1N1B4_501 0.34 -1.2
593,624 - Pf1N1B4_501 0.34 -1.0
593,624 - Pf1N1B4_501 0.34 -0.5
593,986 + Pf1N1B4_501 0.81 +0.1
594,162 + -1.5
594,163 - -1.5
594,180 + -0.2
594,180 + +0.3
594,215 + +0.2
594,215 + +0.2
594,215 + +1.7
594,215 + +1.1
594,215 + +1.5
594,215 + +0.2
594,216 - +0.8
594,216 - -0.2
594,348 - Pf1N1B4_502 0.12 -1.2
594,348 - Pf1N1B4_502 0.12 +0.8
594,361 + Pf1N1B4_502 0.13 +0.5
594,361 + Pf1N1B4_502 0.13 +0.2
594,361 + +1.4
594,361 + Pf1N1B4_502 0.13 +0.8
594,361 + Pf1N1B4_502 0.13 -2.2
594,361 + Pf1N1B4_502 0.13 +0.2
594,361 + +0.8
594,361 + Pf1N1B4_502 0.13 -0.4
594,362 - Pf1N1B4_502 0.13 +0.8
594,362 - Pf1N1B4_502 0.13 -0.2
594,362 - Pf1N1B4_502 0.13 +0.2
594,362 - Pf1N1B4_502 0.13 -0.2
594,365 + Pf1N1B4_502 0.13 -0.2
594,366 - Pf1N1B4_502 0.13 -1.4
594,431 + Pf1N1B4_502 0.18 -0.2
594,432 - Pf1N1B4_502 0.18 +0.1
594,607 + Pf1N1B4_502 0.31 -1.0
594,881 + Pf1N1B4_502 0.52 +0.4
594,881 + Pf1N1B4_502 0.52 -0.8
594,881 + Pf1N1B4_502 0.52 +2.4
594,881 + Pf1N1B4_502 0.52 +1.1
594,881 + Pf1N1B4_502 0.52 +0.8
594,882 - Pf1N1B4_502 0.52 -1.8
594,882 - Pf1N1B4_502 0.52 -0.0
594,968 + Pf1N1B4_502 0.58 -0.2
594,968 + Pf1N1B4_502 0.58 -0.2
594,968 + Pf1N1B4_502 0.58 +1.1
594,969 - Pf1N1B4_502 0.58 +1.8
594,969 - Pf1N1B4_502 0.58 -0.2
594,969 - Pf1N1B4_502 0.58 +0.4
594,969 - Pf1N1B4_502 0.58 +0.1
594,995 + Pf1N1B4_502 0.60 -0.8
594,996 - Pf1N1B4_502 0.60 -2.5
594,996 - Pf1N1B4_502 0.60 +0.5
594,996 - Pf1N1B4_502 0.60 +0.1
595,060 + Pf1N1B4_502 0.65 -0.2

Or see this region's nucleotide sequence