Experiment: nitrite 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4980 and Pf1N1B4_4981 are separated by 9 nucleotides Pf1N1B4_4981 and Pf1N1B4_4982 are separated by 101 nucleotides Pf1N1B4_4982 and Pf1N1B4_4983 are separated by 178 nucleotides
Pf1N1B4_4980 - putative lipoprotein, at 5,519,754 to 5,520,896
_4980
Pf1N1B4_4981 - lipoprotein, putative, at 5,520,906 to 5,521,544
_4981
Pf1N1B4_4982 - Transcriptional regulator, AraC family, at 5,521,646 to 5,522,650
_4982
Pf1N1B4_4983 - Polyhydroxyalkanoic acid synthase, at 5,522,829 to 5,524,586
_4983
Position (kb) 5521
5522
5523 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 5520.819 kb on + strand at 5520.840 kb on + strand at 5520.891 kb on - strand at 5520.991 kb on + strand, within Pf1N1B4_4981 at 5520.991 kb on + strand, within Pf1N1B4_4981 at 5521.018 kb on + strand, within Pf1N1B4_4981 at 5521.019 kb on - strand, within Pf1N1B4_4981 at 5521.081 kb on + strand, within Pf1N1B4_4981 at 5521.082 kb on - strand, within Pf1N1B4_4981 at 5521.130 kb on + strand, within Pf1N1B4_4981 at 5521.139 kb on + strand at 5521.234 kb on + strand, within Pf1N1B4_4981 at 5521.235 kb on - strand at 5521.235 kb on - strand, within Pf1N1B4_4981 at 5521.248 kb on - strand, within Pf1N1B4_4981 at 5521.588 kb on + strand at 5521.588 kb on + strand at 5521.588 kb on + strand at 5521.588 kb on + strand at 5521.589 kb on - strand at 5521.589 kb on - strand at 5521.589 kb on - strand at 5521.634 kb on + strand at 5521.634 kb on + strand at 5521.635 kb on - strand at 5521.635 kb on - strand at 5521.636 kb on + strand at 5521.636 kb on + strand at 5521.636 kb on + strand at 5521.644 kb on + strand at 5521.644 kb on + strand at 5521.648 kb on - strand at 5521.724 kb on - strand at 5521.961 kb on + strand, within Pf1N1B4_4982 at 5521.987 kb on + strand, within Pf1N1B4_4982 at 5521.998 kb on + strand, within Pf1N1B4_4982 at 5522.165 kb on + strand at 5522.166 kb on - strand, within Pf1N1B4_4982 at 5522.166 kb on - strand, within Pf1N1B4_4982 at 5522.614 kb on - strand at 5522.684 kb on - strand at 5522.692 kb on - strand at 5522.835 kb on - strand at 5522.949 kb on - strand at 5523.529 kb on + strand, within Pf1N1B4_4983 at 5523.529 kb on + strand, within Pf1N1B4_4983 at 5523.529 kb on + strand, within Pf1N1B4_4983 at 5523.529 kb on + strand, within Pf1N1B4_4983 at 5523.573 kb on + strand, within Pf1N1B4_4983 at 5523.574 kb on - strand, within Pf1N1B4_4983 at 5523.576 kb on + strand, within Pf1N1B4_4983 at 5523.576 kb on + strand, within Pf1N1B4_4983 at 5523.577 kb on - strand, within Pf1N1B4_4983 at 5523.577 kb on - strand, within Pf1N1B4_4983 at 5523.634 kb on + strand, within Pf1N1B4_4983 at 5523.634 kb on + strand, within Pf1N1B4_4983 at 5523.634 kb on + strand, within Pf1N1B4_4983 at 5523.634 kb on + strand, within Pf1N1B4_4983 at 5523.634 kb on + strand, within Pf1N1B4_4983 at 5523.635 kb on - strand, within Pf1N1B4_4983 at 5523.635 kb on - strand, within Pf1N1B4_4983 at 5523.640 kb on + strand, within Pf1N1B4_4983 at 5523.640 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrite 10 mM remove 5,520,819 + -0.4 5,520,840 + +0.1 5,520,891 - -1.4 5,520,991 + Pf1N1B4_4981 0.13 -0.3 5,520,991 + Pf1N1B4_4981 0.13 +0.1 5,521,018 + Pf1N1B4_4981 0.18 +1.2 5,521,019 - Pf1N1B4_4981 0.18 +0.6 5,521,081 + Pf1N1B4_4981 0.27 -0.9 5,521,082 - Pf1N1B4_4981 0.28 +0.2 5,521,130 + Pf1N1B4_4981 0.35 +1.4 5,521,139 + +0.2 5,521,234 + Pf1N1B4_4981 0.51 -2.6 5,521,235 - +2.1 5,521,235 - Pf1N1B4_4981 0.51 +1.1 5,521,248 - Pf1N1B4_4981 0.54 +1.8 5,521,588 + -0.2 5,521,588 + -0.3 5,521,588 + +0.3 5,521,588 + -0.1 5,521,589 - -0.8 5,521,589 - +0.6 5,521,589 - -0.0 5,521,634 + +2.1 5,521,634 + +0.3 5,521,635 - -1.4 5,521,635 - +2.1 5,521,636 + -0.4 5,521,636 + -1.0 5,521,636 + -0.0 5,521,644 + +0.6 5,521,644 + +2.1 5,521,648 - +0.1 5,521,724 - -3.1 5,521,961 + Pf1N1B4_4982 0.31 +0.6 5,521,987 + Pf1N1B4_4982 0.34 -0.3 5,521,998 + Pf1N1B4_4982 0.35 -1.4 5,522,165 + +0.6 5,522,166 - Pf1N1B4_4982 0.52 -1.3 5,522,166 - Pf1N1B4_4982 0.52 -0.0 5,522,614 - +1.1 5,522,684 - -1.0 5,522,692 - +1.1 5,522,835 - -1.0 5,522,949 - -0.9 5,523,529 + Pf1N1B4_4983 0.40 -0.7 5,523,529 + Pf1N1B4_4983 0.40 +0.6 5,523,529 + Pf1N1B4_4983 0.40 -0.0 5,523,529 + Pf1N1B4_4983 0.40 +0.3 5,523,573 + Pf1N1B4_4983 0.42 -1.4 5,523,574 - Pf1N1B4_4983 0.42 -1.2 5,523,576 + Pf1N1B4_4983 0.42 +0.6 5,523,576 + Pf1N1B4_4983 0.42 +1.9 5,523,577 - Pf1N1B4_4983 0.43 +0.9 5,523,577 - Pf1N1B4_4983 0.43 +0.9 5,523,634 + Pf1N1B4_4983 0.46 +0.6 5,523,634 + Pf1N1B4_4983 0.46 +1.1 5,523,634 + Pf1N1B4_4983 0.46 +1.1 5,523,634 + Pf1N1B4_4983 0.46 -0.4 5,523,634 + Pf1N1B4_4983 0.46 -1.6 5,523,635 - Pf1N1B4_4983 0.46 -1.2 5,523,635 - Pf1N1B4_4983 0.46 -0.0 5,523,640 + Pf1N1B4_4983 0.46 +0.1 5,523,640 + +2.9
Or see this region's nucleotide sequence