Experiment: nitrite 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4728 and Pf1N1B4_4729 are separated by 77 nucleotides Pf1N1B4_4729 and Pf1N1B4_4730 are separated by 282 nucleotides Pf1N1B4_4730 and Pf1N1B4_4731 are separated by 342 nucleotides
Pf1N1B4_4728 - Na+/H+ antiporter NhaB, at 5,224,615 to 5,226,117
_4728
Pf1N1B4_4729 - Hemolysin-type calcium-binding region, at 5,226,195 to 5,227,484
_4729
Pf1N1B4_4730 - FIG00960186: hypothetical protein, at 5,227,767 to 5,228,030
_4730
Pf1N1B4_4731 - Sigma-54 dependent transcriptional regulator, at 5,228,373 to 5,229,698
_4731
Position (kb) 5226
5227
5228 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5225.630 kb on + strand, within Pf1N1B4_4728 at 5225.631 kb on - strand, within Pf1N1B4_4728 at 5225.796 kb on - strand, within Pf1N1B4_4728 at 5225.796 kb on - strand, within Pf1N1B4_4728 at 5225.796 kb on - strand, within Pf1N1B4_4728 at 5225.812 kb on - strand, within Pf1N1B4_4728 at 5225.859 kb on - strand, within Pf1N1B4_4728 at 5226.132 kb on - strand at 5226.135 kb on - strand at 5226.135 kb on - strand at 5226.252 kb on + strand at 5226.496 kb on - strand, within Pf1N1B4_4729 at 5226.496 kb on - strand at 5226.496 kb on - strand, within Pf1N1B4_4729 at 5226.496 kb on - strand, within Pf1N1B4_4729 at 5226.539 kb on + strand, within Pf1N1B4_4729 at 5226.852 kb on - strand at 5226.882 kb on + strand, within Pf1N1B4_4729 at 5227.653 kb on - strand at 5227.704 kb on + strand at 5227.704 kb on + strand at 5227.719 kb on + strand at 5227.719 kb on + strand at 5227.720 kb on - strand at 5227.768 kb on + strand at 5227.799 kb on - strand, within Pf1N1B4_4730 at 5227.930 kb on - strand, within Pf1N1B4_4730 at 5228.045 kb on - strand at 5228.045 kb on - strand at 5228.047 kb on - strand at 5228.047 kb on - strand at 5228.103 kb on - strand at 5228.242 kb on - strand at 5228.248 kb on - strand at 5228.267 kb on - strand at 5228.480 kb on + strand at 5228.480 kb on + strand at 5228.481 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrite 10 mM remove 5,225,630 + Pf1N1B4_4728 0.68 +0.5 5,225,631 - Pf1N1B4_4728 0.68 +1.6 5,225,796 - Pf1N1B4_4728 0.79 +0.0 5,225,796 - Pf1N1B4_4728 0.79 +1.3 5,225,796 - Pf1N1B4_4728 0.79 -1.4 5,225,812 - Pf1N1B4_4728 0.80 +1.3 5,225,859 - Pf1N1B4_4728 0.83 +0.3 5,226,132 - -0.1 5,226,135 - -1.0 5,226,135 - -1.2 5,226,252 + +0.2 5,226,496 - Pf1N1B4_4729 0.23 -1.2 5,226,496 - +0.6 5,226,496 - Pf1N1B4_4729 0.23 -1.0 5,226,496 - Pf1N1B4_4729 0.23 +0.6 5,226,539 + Pf1N1B4_4729 0.27 -2.0 5,226,852 - +2.6 5,226,882 + Pf1N1B4_4729 0.53 +0.6 5,227,653 - -2.9 5,227,704 + -1.0 5,227,704 + -0.4 5,227,719 + -0.4 5,227,719 + -0.1 5,227,720 - -2.0 5,227,768 + -1.0 5,227,799 - Pf1N1B4_4730 0.12 +0.6 5,227,930 - Pf1N1B4_4730 0.62 -0.2 5,228,045 - -0.4 5,228,045 - -0.4 5,228,047 - +0.6 5,228,047 - -1.7 5,228,103 - -0.3 5,228,242 - +0.2 5,228,248 - +1.2 5,228,267 - +0.0 5,228,480 + -0.4 5,228,480 + +0.6 5,228,481 - -0.8
Or see this region's nucleotide sequence