Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_406

Experiment: nitrite 10 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_405 and Pf1N1B4_406 overlap by 8 nucleotidesPf1N1B4_406 and Pf1N1B4_407 are separated by 220 nucleotides Pf1N1B4_405 - Carbohydrate kinase, PfkB family, at 473,297 to 474,226 _405 Pf1N1B4_406 - Purine-cytosine permease, at 474,219 to 475,685 _406 Pf1N1B4_407 - ADP-ribosylglycohydrolase YegU (EC 3.2.-.-), at 475,906 to 476,904 _407 Position (kb) 474 475 476Strain fitness (log2 ratio) -2 -1 0 1 2at 473.256 kb on - strandat 473.256 kb on - strandat 473.256 kb on - strandat 473.312 kb on - strandat 473.818 kb on + strand, within Pf1N1B4_405at 473.819 kb on - strand, within Pf1N1B4_405at 473.832 kb on + strand, within Pf1N1B4_405at 473.833 kb on - strand, within Pf1N1B4_405at 473.833 kb on - strand, within Pf1N1B4_405at 474.060 kb on + strand, within Pf1N1B4_405at 474.061 kb on - strand, within Pf1N1B4_405at 474.281 kb on - strandat 474.470 kb on - strand, within Pf1N1B4_406at 474.511 kb on + strand, within Pf1N1B4_406at 474.512 kb on - strand, within Pf1N1B4_406at 474.512 kb on - strand, within Pf1N1B4_406at 474.512 kb on - strand, within Pf1N1B4_406at 474.515 kb on - strand, within Pf1N1B4_406at 474.515 kb on - strand, within Pf1N1B4_406at 474.515 kb on - strand, within Pf1N1B4_406at 474.631 kb on + strand, within Pf1N1B4_406at 474.631 kb on + strand, within Pf1N1B4_406at 474.631 kb on + strand, within Pf1N1B4_406at 474.631 kb on + strand, within Pf1N1B4_406at 474.632 kb on - strand, within Pf1N1B4_406at 474.657 kb on + strand, within Pf1N1B4_406at 474.676 kb on + strand, within Pf1N1B4_406at 474.728 kb on - strand, within Pf1N1B4_406at 475.135 kb on + strand, within Pf1N1B4_406at 475.135 kb on + strand, within Pf1N1B4_406at 475.135 kb on + strand, within Pf1N1B4_406at 475.135 kb on + strand, within Pf1N1B4_406at 475.135 kb on + strand, within Pf1N1B4_406at 475.135 kb on + strandat 475.136 kb on - strand, within Pf1N1B4_406at 475.136 kb on - strand, within Pf1N1B4_406at 475.136 kb on - strand, within Pf1N1B4_406at 475.136 kb on - strand, within Pf1N1B4_406at 475.136 kb on - strand, within Pf1N1B4_406at 475.136 kb on - strand, within Pf1N1B4_406at 475.136 kb on - strand, within Pf1N1B4_406at 475.563 kb on - strandat 475.685 kb on + strandat 475.685 kb on + strandat 475.685 kb on + strandat 475.685 kb on + strandat 475.686 kb on - strandat 475.686 kb on - strandat 475.686 kb on - strandat 475.686 kb on - strandat 475.740 kb on - strandat 475.794 kb on + strandat 475.795 kb on - strandat 475.795 kb on - strandat 475.798 kb on - strandat 475.837 kb on + strandat 475.861 kb on - strandat 475.864 kb on - strandat 475.864 kb on - strandat 475.902 kb on - strandat 475.966 kb on - strandat 476.184 kb on - strand, within Pf1N1B4_407at 476.256 kb on + strandat 476.490 kb on + strand, within Pf1N1B4_407at 476.491 kb on - strand, within Pf1N1B4_407at 476.491 kb on - strand, within Pf1N1B4_407

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrite 10 mM
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473,256 - -0.7
473,256 - -0.2
473,256 - +0.4
473,312 - -0.2
473,818 + Pf1N1B4_405 0.56 +0.2
473,819 - Pf1N1B4_405 0.56 -0.4
473,832 + Pf1N1B4_405 0.58 +0.2
473,833 - Pf1N1B4_405 0.58 +1.1
473,833 - Pf1N1B4_405 0.58 -0.8
474,060 + Pf1N1B4_405 0.82 -0.1
474,061 - Pf1N1B4_405 0.82 +0.5
474,281 - -0.6
474,470 - Pf1N1B4_406 0.17 -0.9
474,511 + Pf1N1B4_406 0.20 -2.7
474,512 - Pf1N1B4_406 0.20 -0.6
474,512 - Pf1N1B4_406 0.20 -2.4
474,512 - Pf1N1B4_406 0.20 -0.2
474,515 - Pf1N1B4_406 0.20 +0.4
474,515 - Pf1N1B4_406 0.20 -1.0
474,515 - Pf1N1B4_406 0.20 -2.0
474,631 + Pf1N1B4_406 0.28 -0.8
474,631 + Pf1N1B4_406 0.28 +1.8
474,631 + Pf1N1B4_406 0.28 -0.6
474,631 + Pf1N1B4_406 0.28 -1.8
474,632 - Pf1N1B4_406 0.28 -0.4
474,657 + Pf1N1B4_406 0.30 -0.2
474,676 + Pf1N1B4_406 0.31 +0.2
474,728 - Pf1N1B4_406 0.35 +0.0
475,135 + Pf1N1B4_406 0.62 +1.2
475,135 + Pf1N1B4_406 0.62 +0.8
475,135 + Pf1N1B4_406 0.62 +0.8
475,135 + Pf1N1B4_406 0.62 +0.2
475,135 + Pf1N1B4_406 0.62 -1.6
475,135 + +0.0
475,136 - Pf1N1B4_406 0.63 -1.0
475,136 - Pf1N1B4_406 0.63 +0.5
475,136 - Pf1N1B4_406 0.63 -1.8
475,136 - Pf1N1B4_406 0.63 -1.0
475,136 - Pf1N1B4_406 0.63 -2.4
475,136 - Pf1N1B4_406 0.63 -0.7
475,136 - Pf1N1B4_406 0.63 -1.4
475,563 - +0.1
475,685 + +0.2
475,685 + -0.2
475,685 + +0.8
475,685 + -1.6
475,686 - -0.2
475,686 - +0.3
475,686 - -0.8
475,686 - -0.6
475,740 - -2.0
475,794 + +0.3
475,795 - -0.7
475,795 - +0.2
475,798 - +0.8
475,837 + +0.8
475,861 - -0.4
475,864 - +0.3
475,864 - -2.4
475,902 - +0.4
475,966 - -0.8
476,184 - Pf1N1B4_407 0.28 +0.9
476,256 + +0.2
476,490 + Pf1N1B4_407 0.58 -0.2
476,491 - Pf1N1B4_407 0.59 +0.5
476,491 - Pf1N1B4_407 0.59 -0.2

Or see this region's nucleotide sequence