Experiment: nitrite 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4046 and Pf1N1B4_4047 are separated by 127 nucleotides Pf1N1B4_4047 and Pf1N1B4_4048 overlap by 4 nucleotides Pf1N1B4_4048 and Pf1N1B4_4049 are separated by 59 nucleotides Pf1N1B4_4049 and Pf1N1B4_4050 are separated by 213 nucleotides Pf1N1B4_4050 and Pf1N1B4_4051 are separated by 38 nucleotides
Pf1N1B4_4046 - Adenylosuccinate lyase (EC 4.3.2.2), at 4,498,338 to 4,499,708
_4046
Pf1N1B4_4047 - FIG002903: a protein of unknown function perhaps involved in purine metabolism, at 4,499,836 to 4,500,459
_4047
Pf1N1B4_4048 - tRNA-specific 2-thiouridylase MnmA, at 4,500,456 to 4,501,589
_4048
Pf1N1B4_4049 - Nudix-like NDP and NTP phosphohydrolase YmfB, at 4,501,649 to 4,502,095
_4049
Pf1N1B4_4050 - Isocitrate dehydrogenase [NADP] (EC 1.1.1.42); Monomeric isocitrate dehydrogenase [NADP] (EC 1.1.1.42), at 4,502,309 to 4,502,422
_4050
Pf1N1B4_4051 - Isocitrate dehydrogenase [NADP] (EC 1.1.1.42); Monomeric isocitrate dehydrogenase [NADP] (EC 1.1.1.42), at 4,502,461 to 4,504,533
_4051
Position (kb)
4500
4501
4502 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4499.795 kb on - strand at 4499.795 kb on - strand at 4499.795 kb on - strand at 4499.811 kb on + strand at 4499.821 kb on + strand at 4499.868 kb on + strand at 4499.950 kb on + strand at 4499.954 kb on - strand, within Pf1N1B4_4047 at 4499.954 kb on - strand, within Pf1N1B4_4047 at 4499.954 kb on - strand, within Pf1N1B4_4047 at 4500.053 kb on + strand, within Pf1N1B4_4047 at 4500.053 kb on + strand, within Pf1N1B4_4047 at 4500.054 kb on - strand, within Pf1N1B4_4047 at 4500.054 kb on - strand, within Pf1N1B4_4047 at 4500.054 kb on - strand, within Pf1N1B4_4047 at 4500.299 kb on + strand, within Pf1N1B4_4047 at 4500.299 kb on + strand, within Pf1N1B4_4047 at 4500.299 kb on + strand, within Pf1N1B4_4047 at 4500.300 kb on - strand, within Pf1N1B4_4047 at 4500.300 kb on - strand, within Pf1N1B4_4047 at 4501.709 kb on - strand at 4501.709 kb on - strand, within Pf1N1B4_4049 at 4501.806 kb on + strand, within Pf1N1B4_4049 at 4501.854 kb on + strand, within Pf1N1B4_4049 at 4501.854 kb on + strand, within Pf1N1B4_4049 at 4501.855 kb on - strand, within Pf1N1B4_4049 at 4501.881 kb on + strand, within Pf1N1B4_4049 at 4501.881 kb on + strand, within Pf1N1B4_4049 at 4501.881 kb on + strand, within Pf1N1B4_4049 at 4501.887 kb on + strand at 4501.887 kb on + strand, within Pf1N1B4_4049 at 4501.887 kb on + strand, within Pf1N1B4_4049 at 4501.887 kb on + strand, within Pf1N1B4_4049 at 4501.887 kb on + strand, within Pf1N1B4_4049 at 4502.239 kb on + strand at 4502.239 kb on + strand at 4502.240 kb on - strand at 4502.240 kb on - strand at 4502.240 kb on - strand at 4502.240 kb on - strand at 4502.240 kb on - strand at 4502.240 kb on - strand at 4502.240 kb on - strand at 4502.240 kb on - strand at 4502.240 kb on - strand at 4502.240 kb on - strand at 4502.240 kb on - strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.244 kb on - strand at 4502.244 kb on - strand at 4502.244 kb on - strand at 4502.244 kb on - strand at 4502.244 kb on - strand at 4502.247 kb on + strand at 4502.247 kb on + strand at 4502.247 kb on + strand at 4502.247 kb on + strand at 4502.248 kb on - strand at 4502.248 kb on - strand at 4502.302 kb on + strand at 4502.310 kb on + strand at 4502.551 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrite 10 mM remove 4,499,795 - -0.1 4,499,795 - +0.9 4,499,795 - +1.7 4,499,811 + +1.9 4,499,821 + -0.9 4,499,868 + -0.9 4,499,950 + +1.3 4,499,954 - Pf1N1B4_4047 0.19 -1.6 4,499,954 - Pf1N1B4_4047 0.19 +0.6 4,499,954 - Pf1N1B4_4047 0.19 -2.6 4,500,053 + Pf1N1B4_4047 0.35 -1.1 4,500,053 + Pf1N1B4_4047 0.35 +0.7 4,500,054 - Pf1N1B4_4047 0.35 -0.1 4,500,054 - Pf1N1B4_4047 0.35 -0.1 4,500,054 - Pf1N1B4_4047 0.35 -1.4 4,500,299 + Pf1N1B4_4047 0.74 -0.3 4,500,299 + Pf1N1B4_4047 0.74 -1.3 4,500,299 + Pf1N1B4_4047 0.74 -1.1 4,500,300 - Pf1N1B4_4047 0.74 -0.7 4,500,300 - Pf1N1B4_4047 0.74 -0.1 4,501,709 - +2.3 4,501,709 - Pf1N1B4_4049 0.13 -1.1 4,501,806 + Pf1N1B4_4049 0.35 -0.6 4,501,854 + Pf1N1B4_4049 0.46 +0.6 4,501,854 + Pf1N1B4_4049 0.46 -0.6 4,501,855 - Pf1N1B4_4049 0.46 +1.2 4,501,881 + Pf1N1B4_4049 0.52 +0.7 4,501,881 + Pf1N1B4_4049 0.52 +1.5 4,501,881 + Pf1N1B4_4049 0.52 -0.9 4,501,887 + +0.7 4,501,887 + Pf1N1B4_4049 0.53 +0.1 4,501,887 + Pf1N1B4_4049 0.53 -1.0 4,501,887 + Pf1N1B4_4049 0.53 +0.2 4,501,887 + Pf1N1B4_4049 0.53 -0.5 4,502,239 + -0.6 4,502,239 + +0.2 4,502,240 - -0.5 4,502,240 - -1.5 4,502,240 - -1.1 4,502,240 - +0.2 4,502,240 - -0.0 4,502,240 - +0.7 4,502,240 - +0.7 4,502,240 - -1.2 4,502,240 - +0.7 4,502,240 - -0.3 4,502,240 - +0.1 4,502,243 + -0.2 4,502,243 + +0.7 4,502,243 + -0.1 4,502,243 + +0.7 4,502,243 + +1.7 4,502,243 + -0.3 4,502,243 + -0.3 4,502,243 + +0.4 4,502,243 + +1.5 4,502,243 + +0.7 4,502,243 + +1.5 4,502,243 + +0.3 4,502,243 + +1.3 4,502,243 + -1.6 4,502,244 - -1.8 4,502,244 - +1.2 4,502,244 - +0.1 4,502,244 - +2.7 4,502,244 - -0.5 4,502,247 + -0.2 4,502,247 + -1.3 4,502,247 + +0.7 4,502,247 + +0.8 4,502,248 - -1.9 4,502,248 - +0.4 4,502,302 + -0.9 4,502,310 + -3.2 4,502,551 - -1.9
Or see this region's nucleotide sequence