Experiment: nitrite 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_398 and Pf1N1B4_399 are separated by 46 nucleotides Pf1N1B4_399 and Pf1N1B4_400 are separated by 19 nucleotides
Pf1N1B4_398 - Galactonate dehydratase (EC 4.2.1.6), at 467,549 to 468,697
_398
Pf1N1B4_399 - 2-dehydro-3-deoxyphosphogalactonate aldolase (EC 4.1.2.21), at 468,744 to 469,364
_399
Pf1N1B4_400 - 2-dehydro-3-deoxygalactonokinase (EC 2.7.1.58), at 469,384 to 470,376
_400
Position (kb) 468
469
470 Strain fitness (log2 ratio) -2
-1
0
1 at 467.748 kb on + strand, within Pf1N1B4_398 at 467.748 kb on + strand, within Pf1N1B4_398 at 467.748 kb on + strand, within Pf1N1B4_398 at 467.748 kb on + strand, within Pf1N1B4_398 at 467.749 kb on - strand, within Pf1N1B4_398 at 467.749 kb on - strand, within Pf1N1B4_398 at 467.865 kb on + strand, within Pf1N1B4_398 at 467.866 kb on - strand, within Pf1N1B4_398 at 467.866 kb on - strand, within Pf1N1B4_398 at 467.946 kb on + strand, within Pf1N1B4_398 at 467.946 kb on + strand, within Pf1N1B4_398 at 467.946 kb on + strand, within Pf1N1B4_398 at 467.947 kb on - strand, within Pf1N1B4_398 at 468.091 kb on - strand, within Pf1N1B4_398 at 468.130 kb on + strand, within Pf1N1B4_398 at 468.348 kb on + strand, within Pf1N1B4_398 at 468.349 kb on - strand, within Pf1N1B4_398 at 468.349 kb on - strand, within Pf1N1B4_398 at 468.480 kb on + strand, within Pf1N1B4_398 at 468.480 kb on + strand, within Pf1N1B4_398 at 468.480 kb on + strand, within Pf1N1B4_398 at 468.495 kb on + strand, within Pf1N1B4_398 at 468.546 kb on + strand, within Pf1N1B4_398 at 468.547 kb on - strand at 468.547 kb on - strand, within Pf1N1B4_398 at 468.699 kb on + strand at 468.700 kb on - strand at 468.700 kb on - strand at 468.700 kb on - strand at 468.700 kb on - strand at 468.712 kb on - strand at 468.712 kb on - strand at 468.712 kb on - strand at 468.712 kb on - strand at 468.712 kb on - strand at 468.714 kb on + strand at 468.714 kb on + strand at 468.715 kb on - strand at 468.715 kb on - strand at 468.715 kb on - strand at 468.715 kb on - strand at 468.715 kb on - strand at 468.715 kb on - strand at 468.715 kb on - strand at 468.715 kb on - strand at 468.772 kb on + strand at 468.772 kb on + strand at 468.772 kb on + strand at 468.772 kb on + strand at 468.773 kb on - strand at 468.773 kb on - strand at 468.773 kb on - strand at 468.773 kb on - strand at 469.196 kb on + strand, within Pf1N1B4_399 at 469.197 kb on - strand, within Pf1N1B4_399 at 469.214 kb on + strand, within Pf1N1B4_399 at 469.222 kb on + strand, within Pf1N1B4_399 at 469.223 kb on - strand, within Pf1N1B4_399 at 469.239 kb on - strand, within Pf1N1B4_399 at 469.274 kb on - strand, within Pf1N1B4_399 at 469.629 kb on - strand, within Pf1N1B4_400 at 470.324 kb on + strand at 470.324 kb on + strand at 470.324 kb on + strand at 470.324 kb on + strand at 470.325 kb on - strand at 470.332 kb on + strand at 470.332 kb on + strand at 470.333 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrite 10 mM remove 467,748 + Pf1N1B4_398 0.17 +0.2 467,748 + Pf1N1B4_398 0.17 -1.4 467,748 + Pf1N1B4_398 0.17 -0.2 467,748 + Pf1N1B4_398 0.17 +1.3 467,749 - Pf1N1B4_398 0.17 -1.0 467,749 - Pf1N1B4_398 0.17 -1.0 467,865 + Pf1N1B4_398 0.28 +1.6 467,866 - Pf1N1B4_398 0.28 +0.0 467,866 - Pf1N1B4_398 0.28 -0.5 467,946 + Pf1N1B4_398 0.35 -1.1 467,946 + Pf1N1B4_398 0.35 +1.3 467,946 + Pf1N1B4_398 0.35 -0.5 467,947 - Pf1N1B4_398 0.35 -0.7 468,091 - Pf1N1B4_398 0.47 -1.6 468,130 + Pf1N1B4_398 0.51 -0.8 468,348 + Pf1N1B4_398 0.70 -1.0 468,349 - Pf1N1B4_398 0.70 -0.2 468,349 - Pf1N1B4_398 0.70 -0.1 468,480 + Pf1N1B4_398 0.81 -0.2 468,480 + Pf1N1B4_398 0.81 -0.2 468,480 + Pf1N1B4_398 0.81 -0.6 468,495 + Pf1N1B4_398 0.82 -0.2 468,546 + Pf1N1B4_398 0.87 -0.2 468,547 - +0.8 468,547 - Pf1N1B4_398 0.87 +0.8 468,699 + +0.5 468,700 - -0.2 468,700 - -2.0 468,700 - +1.1 468,700 - +0.2 468,712 - +0.4 468,712 - -0.5 468,712 - -0.2 468,712 - +1.6 468,712 - -0.8 468,714 + +0.4 468,714 + -0.2 468,715 - -1.2 468,715 - -0.2 468,715 - -1.0 468,715 - -0.1 468,715 - -0.5 468,715 - -0.8 468,715 - +0.8 468,715 - -0.0 468,772 + -0.2 468,772 + +1.5 468,772 + -0.2 468,772 + +0.1 468,773 - -1.2 468,773 - -0.5 468,773 - +0.4 468,773 - +0.1 469,196 + Pf1N1B4_399 0.73 -1.8 469,197 - Pf1N1B4_399 0.73 +0.0 469,214 + Pf1N1B4_399 0.76 -0.8 469,222 + Pf1N1B4_399 0.77 -1.2 469,223 - Pf1N1B4_399 0.77 -0.2 469,239 - Pf1N1B4_399 0.80 -0.2 469,274 - Pf1N1B4_399 0.85 -0.6 469,629 - Pf1N1B4_400 0.25 -0.4 470,324 + -1.2 470,324 + -0.2 470,324 + -0.2 470,324 + +0.0 470,325 - -1.6 470,332 + -0.6 470,332 + -0.6 470,333 - +0.2
Or see this region's nucleotide sequence