Experiment: nitrite 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_354 and Pf1N1B4_355 are separated by 103 nucleotides Pf1N1B4_355 and Pf1N1B4_356 are separated by 71 nucleotides Pf1N1B4_356 and Pf1N1B4_357 are separated by 4 nucleotides
Pf1N1B4_354 - Methylthioribose-1-phosphate isomerase (EC 5.3.1.23), at 420,767 to 421,849
_354
Pf1N1B4_355 - S-adenosylhomocysteine deaminase (EC 3.5.4.28); Methylthioadenosine deaminase, at 421,953 to 423,287
_355
Pf1N1B4_356 - 3-demethylubiquinone-9 3-methyltransferase (EC 2.1.1.64), at 423,359 to 424,057
_356
Pf1N1B4_357 - Similar to phosphoglycolate phosphatase, clustered with ubiquinone biosynthesis SAM-dependent O-methyltransferase, at 424,062 to 424,733
_357
Position (kb) 421
422
423
424 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 421.016 kb on + strand, within Pf1N1B4_354 at 421.017 kb on - strand, within Pf1N1B4_354 at 421.762 kb on + strand at 421.763 kb on - strand at 421.763 kb on - strand at 421.763 kb on - strand at 421.780 kb on - strand at 421.850 kb on - strand at 421.880 kb on + strand at 421.882 kb on + strand at 421.882 kb on + strand at 421.882 kb on + strand at 421.883 kb on - strand at 421.912 kb on + strand at 421.912 kb on + strand at 421.912 kb on + strand at 421.913 kb on - strand at 421.913 kb on - strand at 421.913 kb on - strand at 421.913 kb on - strand at 421.927 kb on + strand at 421.927 kb on + strand at 421.928 kb on - strand at 421.928 kb on - strand at 422.005 kb on + strand at 422.071 kb on + strand at 422.071 kb on + strand at 422.071 kb on + strand at 422.071 kb on + strand at 422.071 kb on + strand at 422.071 kb on + strand at 422.072 kb on - strand at 422.267 kb on - strand, within Pf1N1B4_355 at 422.349 kb on + strand at 422.349 kb on + strand, within Pf1N1B4_355 at 422.349 kb on + strand, within Pf1N1B4_355 at 422.349 kb on + strand, within Pf1N1B4_355 at 422.349 kb on + strand, within Pf1N1B4_355 at 422.349 kb on + strand, within Pf1N1B4_355 at 422.349 kb on + strand, within Pf1N1B4_355 at 422.349 kb on + strand, within Pf1N1B4_355 at 422.349 kb on + strand, within Pf1N1B4_355 at 422.349 kb on + strand, within Pf1N1B4_355 at 422.349 kb on + strand at 422.350 kb on - strand, within Pf1N1B4_355 at 422.380 kb on + strand, within Pf1N1B4_355 at 422.380 kb on + strand, within Pf1N1B4_355 at 422.380 kb on + strand, within Pf1N1B4_355 at 422.380 kb on + strand, within Pf1N1B4_355 at 422.381 kb on - strand, within Pf1N1B4_355 at 422.384 kb on + strand, within Pf1N1B4_355 at 422.384 kb on + strand, within Pf1N1B4_355 at 422.384 kb on + strand, within Pf1N1B4_355 at 422.384 kb on + strand, within Pf1N1B4_355 at 422.384 kb on + strand, within Pf1N1B4_355 at 422.384 kb on + strand, within Pf1N1B4_355 at 422.384 kb on + strand, within Pf1N1B4_355 at 422.384 kb on + strand, within Pf1N1B4_355 at 422.385 kb on - strand, within Pf1N1B4_355 at 422.385 kb on - strand, within Pf1N1B4_355 at 422.385 kb on - strand, within Pf1N1B4_355 at 422.385 kb on - strand, within Pf1N1B4_355 at 422.385 kb on - strand, within Pf1N1B4_355 at 422.385 kb on - strand, within Pf1N1B4_355 at 422.385 kb on - strand, within Pf1N1B4_355 at 422.385 kb on - strand, within Pf1N1B4_355 at 422.482 kb on + strand, within Pf1N1B4_355 at 422.483 kb on - strand, within Pf1N1B4_355 at 422.541 kb on + strand, within Pf1N1B4_355 at 422.541 kb on + strand, within Pf1N1B4_355 at 422.541 kb on + strand, within Pf1N1B4_355 at 422.542 kb on - strand, within Pf1N1B4_355 at 422.542 kb on - strand, within Pf1N1B4_355 at 422.609 kb on + strand, within Pf1N1B4_355 at 422.609 kb on + strand, within Pf1N1B4_355 at 422.609 kb on + strand, within Pf1N1B4_355 at 422.609 kb on + strand, within Pf1N1B4_355 at 422.610 kb on - strand, within Pf1N1B4_355 at 422.610 kb on - strand, within Pf1N1B4_355 at 422.610 kb on - strand, within Pf1N1B4_355 at 422.610 kb on - strand, within Pf1N1B4_355 at 422.764 kb on + strand, within Pf1N1B4_355 at 422.764 kb on + strand, within Pf1N1B4_355 at 422.765 kb on - strand, within Pf1N1B4_355 at 422.765 kb on - strand, within Pf1N1B4_355 at 422.771 kb on + strand, within Pf1N1B4_355 at 422.771 kb on + strand, within Pf1N1B4_355 at 422.771 kb on + strand, within Pf1N1B4_355 at 422.771 kb on + strand, within Pf1N1B4_355 at 422.771 kb on + strand, within Pf1N1B4_355 at 422.771 kb on + strand, within Pf1N1B4_355 at 422.771 kb on + strand, within Pf1N1B4_355 at 422.771 kb on + strand, within Pf1N1B4_355 at 422.895 kb on - strand, within Pf1N1B4_355 at 423.114 kb on + strand, within Pf1N1B4_355 at 423.115 kb on - strand, within Pf1N1B4_355 at 423.138 kb on + strand, within Pf1N1B4_355 at 423.138 kb on + strand, within Pf1N1B4_355 at 423.138 kb on + strand, within Pf1N1B4_355 at 423.138 kb on + strand at 423.138 kb on + strand, within Pf1N1B4_355 at 423.138 kb on + strand, within Pf1N1B4_355 at 423.138 kb on + strand, within Pf1N1B4_355 at 423.138 kb on + strand, within Pf1N1B4_355 at 423.139 kb on - strand, within Pf1N1B4_355 at 423.139 kb on - strand, within Pf1N1B4_355 at 423.139 kb on - strand, within Pf1N1B4_355 at 423.139 kb on - strand, within Pf1N1B4_355 at 423.139 kb on - strand, within Pf1N1B4_355 at 423.139 kb on - strand, within Pf1N1B4_355 at 423.139 kb on - strand, within Pf1N1B4_355 at 423.139 kb on - strand, within Pf1N1B4_355 at 423.139 kb on - strand, within Pf1N1B4_355 at 423.139 kb on - strand, within Pf1N1B4_355 at 423.139 kb on - strand, within Pf1N1B4_355 at 423.285 kb on + strand at 423.285 kb on + strand at 423.299 kb on + strand at 423.299 kb on + strand at 423.299 kb on + strand at 423.299 kb on + strand at 423.299 kb on + strand at 423.299 kb on + strand at 423.299 kb on + strand at 423.299 kb on + strand at 423.299 kb on + strand at 423.344 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrite 10 mM remove 421,016 + Pf1N1B4_354 0.23 +0.3 421,017 - Pf1N1B4_354 0.23 -1.1 421,762 + -0.8 421,763 - -0.8 421,763 - +0.8 421,763 - -0.2 421,780 - +0.6 421,850 - -0.2 421,880 + +0.8 421,882 + -1.6 421,882 + +1.2 421,882 + +1.0 421,883 - -0.2 421,912 + +0.2 421,912 + +0.3 421,912 + +0.3 421,913 - -0.2 421,913 - +0.0 421,913 - -1.6 421,913 - +1.1 421,927 + -0.2 421,927 + -0.8 421,928 - +0.8 421,928 - -1.2 422,005 + -0.2 422,071 + -0.7 422,071 + +1.2 422,071 + -0.6 422,071 + -0.0 422,071 + +0.2 422,071 + -0.6 422,072 - -1.2 422,267 - Pf1N1B4_355 0.24 -1.8 422,349 + -1.2 422,349 + Pf1N1B4_355 0.30 -2.0 422,349 + Pf1N1B4_355 0.30 -0.8 422,349 + Pf1N1B4_355 0.30 +0.1 422,349 + Pf1N1B4_355 0.30 -1.4 422,349 + Pf1N1B4_355 0.30 -0.5 422,349 + Pf1N1B4_355 0.30 -0.5 422,349 + Pf1N1B4_355 0.30 +0.2 422,349 + Pf1N1B4_355 0.30 -1.8 422,349 + Pf1N1B4_355 0.30 +0.8 422,349 + -0.8 422,350 - Pf1N1B4_355 0.30 +0.5 422,380 + Pf1N1B4_355 0.32 +0.5 422,380 + Pf1N1B4_355 0.32 -0.9 422,380 + Pf1N1B4_355 0.32 +0.8 422,380 + Pf1N1B4_355 0.32 +1.8 422,381 - Pf1N1B4_355 0.32 -1.2 422,384 + Pf1N1B4_355 0.32 -2.0 422,384 + Pf1N1B4_355 0.32 -0.5 422,384 + Pf1N1B4_355 0.32 -0.1 422,384 + Pf1N1B4_355 0.32 +2.1 422,384 + Pf1N1B4_355 0.32 -0.0 422,384 + Pf1N1B4_355 0.32 -1.2 422,384 + Pf1N1B4_355 0.32 -2.8 422,384 + Pf1N1B4_355 0.32 -0.2 422,385 - Pf1N1B4_355 0.32 -0.2 422,385 - Pf1N1B4_355 0.32 -2.8 422,385 - Pf1N1B4_355 0.32 -1.8 422,385 - Pf1N1B4_355 0.32 +0.8 422,385 - Pf1N1B4_355 0.32 +0.2 422,385 - Pf1N1B4_355 0.32 +0.2 422,385 - Pf1N1B4_355 0.32 -2.0 422,385 - Pf1N1B4_355 0.32 +0.2 422,482 + Pf1N1B4_355 0.40 +0.8 422,483 - Pf1N1B4_355 0.40 +0.2 422,541 + Pf1N1B4_355 0.44 -1.8 422,541 + Pf1N1B4_355 0.44 +0.1 422,541 + Pf1N1B4_355 0.44 +0.0 422,542 - Pf1N1B4_355 0.44 -1.0 422,542 - Pf1N1B4_355 0.44 -1.5 422,609 + Pf1N1B4_355 0.49 +0.4 422,609 + Pf1N1B4_355 0.49 -2.2 422,609 + Pf1N1B4_355 0.49 +1.4 422,609 + Pf1N1B4_355 0.49 -1.2 422,610 - Pf1N1B4_355 0.49 -0.2 422,610 - Pf1N1B4_355 0.49 -0.2 422,610 - Pf1N1B4_355 0.49 -1.2 422,610 - Pf1N1B4_355 0.49 +0.8 422,764 + Pf1N1B4_355 0.61 +0.2 422,764 + Pf1N1B4_355 0.61 +1.2 422,765 - Pf1N1B4_355 0.61 +1.5 422,765 - Pf1N1B4_355 0.61 -2.0 422,771 + Pf1N1B4_355 0.61 -1.0 422,771 + Pf1N1B4_355 0.61 -1.0 422,771 + Pf1N1B4_355 0.61 -0.8 422,771 + Pf1N1B4_355 0.61 +0.4 422,771 + Pf1N1B4_355 0.61 -1.2 422,771 + Pf1N1B4_355 0.61 +1.2 422,771 + Pf1N1B4_355 0.61 +0.8 422,771 + Pf1N1B4_355 0.61 -0.2 422,895 - Pf1N1B4_355 0.71 +0.8 423,114 + Pf1N1B4_355 0.87 -0.8 423,115 - Pf1N1B4_355 0.87 -1.3 423,138 + Pf1N1B4_355 0.89 -1.0 423,138 + Pf1N1B4_355 0.89 -0.2 423,138 + Pf1N1B4_355 0.89 -0.8 423,138 + +0.8 423,138 + Pf1N1B4_355 0.89 -1.0 423,138 + Pf1N1B4_355 0.89 -0.5 423,138 + Pf1N1B4_355 0.89 +0.8 423,138 + Pf1N1B4_355 0.89 -2.0 423,139 - Pf1N1B4_355 0.89 -1.8 423,139 - Pf1N1B4_355 0.89 -1.0 423,139 - Pf1N1B4_355 0.89 -0.2 423,139 - Pf1N1B4_355 0.89 +0.8 423,139 - Pf1N1B4_355 0.89 -2.4 423,139 - Pf1N1B4_355 0.89 -0.8 423,139 - Pf1N1B4_355 0.89 +0.0 423,139 - Pf1N1B4_355 0.89 -1.8 423,139 - Pf1N1B4_355 0.89 -0.8 423,139 - Pf1N1B4_355 0.89 -0.8 423,139 - Pf1N1B4_355 0.89 -1.0 423,285 + -0.1 423,285 + -0.8 423,299 + -0.2 423,299 + +1.0 423,299 + +2.2 423,299 + -0.2 423,299 + +0.4 423,299 + -0.8 423,299 + +0.8 423,299 + +1.4 423,299 + -3.4 423,344 + +0.5
Or see this region's nucleotide sequence