Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_3168

Experiment: nitrite 10 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_3167 and Pf1N1B4_3168 are separated by 141 nucleotidesPf1N1B4_3168 and Pf1N1B4_3169 are separated by 44 nucleotidesPf1N1B4_3169 and Pf1N1B4_3170 are separated by 167 nucleotides Pf1N1B4_3167 - Prolyl-tRNA synthetase (EC 6.1.1.15), bacterial type, at 3,549,122 to 3,550,837 _3167 Pf1N1B4_3168 - AmpG permease, at 3,550,979 to 3,552,538 _3168 Pf1N1B4_3169 - methylated-DNA--protein-cysteine methyltransferase-related protein, at 3,552,583 to 3,552,936 _3169 Pf1N1B4_3170 - Peptide chain release factor RF-3, at 3,553,104 to 3,554,111 _3170 Position (kb) 3550 3551 3552 3553Strain fitness (log2 ratio) -2 -1 0 1 2 3at 3550.885 kb on - strandat 3550.973 kb on - strandat 3551.141 kb on - strand, within Pf1N1B4_3168at 3551.466 kb on - strand, within Pf1N1B4_3168at 3551.691 kb on + strand, within Pf1N1B4_3168at 3551.692 kb on - strand, within Pf1N1B4_3168at 3551.987 kb on - strand, within Pf1N1B4_3168at 3551.988 kb on + strand, within Pf1N1B4_3168at 3551.989 kb on - strandat 3551.989 kb on - strand, within Pf1N1B4_3168at 3551.989 kb on - strand, within Pf1N1B4_3168at 3552.075 kb on + strand, within Pf1N1B4_3168at 3552.445 kb on - strandat 3552.624 kb on + strand, within Pf1N1B4_3169at 3552.836 kb on + strand, within Pf1N1B4_3169at 3552.837 kb on - strand, within Pf1N1B4_3169at 3552.878 kb on + strand, within Pf1N1B4_3169at 3552.878 kb on + strand, within Pf1N1B4_3169at 3552.878 kb on + strand, within Pf1N1B4_3169at 3552.879 kb on - strand, within Pf1N1B4_3169at 3552.879 kb on - strand, within Pf1N1B4_3169at 3552.879 kb on - strand, within Pf1N1B4_3169at 3552.976 kb on + strandat 3552.981 kb on + strandat 3552.982 kb on - strandat 3552.982 kb on - strandat 3553.041 kb on + strandat 3553.042 kb on - strandat 3553.042 kb on - strandat 3553.103 kb on + strandat 3553.104 kb on - strandat 3553.104 kb on - strandat 3553.104 kb on - strandat 3553.213 kb on + strand, within Pf1N1B4_3170at 3553.214 kb on - strand, within Pf1N1B4_3170at 3553.329 kb on + strand, within Pf1N1B4_3170at 3553.329 kb on + strand, within Pf1N1B4_3170at 3553.329 kb on + strand, within Pf1N1B4_3170at 3553.329 kb on + strand, within Pf1N1B4_3170at 3553.329 kb on + strand, within Pf1N1B4_3170at 3553.329 kb on + strand, within Pf1N1B4_3170at 3553.330 kb on - strand, within Pf1N1B4_3170at 3553.330 kb on - strand, within Pf1N1B4_3170at 3553.330 kb on - strand, within Pf1N1B4_3170at 3553.330 kb on - strand, within Pf1N1B4_3170at 3553.330 kb on - strand, within Pf1N1B4_3170at 3553.330 kb on - strand, within Pf1N1B4_3170at 3553.330 kb on - strand, within Pf1N1B4_3170at 3553.468 kb on + strand, within Pf1N1B4_3170at 3553.468 kb on + strand, within Pf1N1B4_3170at 3553.469 kb on - strand, within Pf1N1B4_3170at 3553.530 kb on + strand, within Pf1N1B4_3170at 3553.530 kb on + strand, within Pf1N1B4_3170at 3553.530 kb on + strand, within Pf1N1B4_3170at 3553.530 kb on + strand, within Pf1N1B4_3170at 3553.530 kb on + strand, within Pf1N1B4_3170at 3553.531 kb on - strand, within Pf1N1B4_3170at 3553.531 kb on - strand, within Pf1N1B4_3170at 3553.531 kb on - strand, within Pf1N1B4_3170

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrite 10 mM
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3,550,885 - -0.5
3,550,973 - -0.1
3,551,141 - Pf1N1B4_3168 0.10 +1.9
3,551,466 - Pf1N1B4_3168 0.31 -0.7
3,551,691 + Pf1N1B4_3168 0.46 -1.9
3,551,692 - Pf1N1B4_3168 0.46 -0.4
3,551,987 - Pf1N1B4_3168 0.65 -0.7
3,551,988 + Pf1N1B4_3168 0.65 -0.9
3,551,989 - +0.9
3,551,989 - Pf1N1B4_3168 0.65 -0.1
3,551,989 - Pf1N1B4_3168 0.65 -0.5
3,552,075 + Pf1N1B4_3168 0.70 -1.5
3,552,445 - -0.7
3,552,624 + Pf1N1B4_3169 0.12 +1.1
3,552,836 + Pf1N1B4_3169 0.71 -0.1
3,552,837 - Pf1N1B4_3169 0.72 +0.5
3,552,878 + Pf1N1B4_3169 0.83 +0.9
3,552,878 + Pf1N1B4_3169 0.83 -0.7
3,552,878 + Pf1N1B4_3169 0.83 +2.5
3,552,879 - Pf1N1B4_3169 0.84 +0.9
3,552,879 - Pf1N1B4_3169 0.84 +0.4
3,552,879 - Pf1N1B4_3169 0.84 +0.9
3,552,976 + +0.9
3,552,981 + +2.9
3,552,982 - +0.4
3,552,982 - +0.9
3,553,041 + +0.1
3,553,042 - -0.1
3,553,042 - +0.9
3,553,103 + +0.2
3,553,104 - +0.3
3,553,104 - +0.1
3,553,104 - +1.1
3,553,213 + Pf1N1B4_3170 0.11 +0.2
3,553,214 - Pf1N1B4_3170 0.11 -1.7
3,553,329 + Pf1N1B4_3170 0.22 +1.9
3,553,329 + Pf1N1B4_3170 0.22 +1.5
3,553,329 + Pf1N1B4_3170 0.22 -0.7
3,553,329 + Pf1N1B4_3170 0.22 +0.2
3,553,329 + Pf1N1B4_3170 0.22 -0.7
3,553,329 + Pf1N1B4_3170 0.22 +1.5
3,553,330 - Pf1N1B4_3170 0.22 +0.3
3,553,330 - Pf1N1B4_3170 0.22 -0.7
3,553,330 - Pf1N1B4_3170 0.22 +0.9
3,553,330 - Pf1N1B4_3170 0.22 +0.1
3,553,330 - Pf1N1B4_3170 0.22 -1.1
3,553,330 - Pf1N1B4_3170 0.22 +1.9
3,553,330 - Pf1N1B4_3170 0.22 +0.1
3,553,468 + Pf1N1B4_3170 0.36 +0.9
3,553,468 + Pf1N1B4_3170 0.36 -0.4
3,553,469 - Pf1N1B4_3170 0.36 +0.0
3,553,530 + Pf1N1B4_3170 0.42 +1.1
3,553,530 + Pf1N1B4_3170 0.42 +0.9
3,553,530 + Pf1N1B4_3170 0.42 +0.9
3,553,530 + Pf1N1B4_3170 0.42 +0.2
3,553,530 + Pf1N1B4_3170 0.42 -1.9
3,553,531 - Pf1N1B4_3170 0.42 +0.5
3,553,531 - Pf1N1B4_3170 0.42 -1.5
3,553,531 - Pf1N1B4_3170 0.42 -0.7

Or see this region's nucleotide sequence