Experiment: nitrite 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2385 and Pf1N1B4_2386 are separated by 325 nucleotides Pf1N1B4_2386 and Pf1N1B4_2387 are separated by 205 nucleotides
Pf1N1B4_2385 - Adenosylhomocysteinase (EC 3.3.1.1), at 2,702,566 to 2,703,975
_2385
Pf1N1B4_2386 - cytosolic long-chain acyl-CoA thioester hydrolase family protein, at 2,704,301 to 2,704,705
_2386
Pf1N1B4_2387 - Putative transport, at 2,704,911 to 2,705,798
_2387
Position (kb)
2704
2705 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2703.993 kb on - strand at 2703.993 kb on - strand at 2703.998 kb on - strand at 2703.998 kb on - strand at 2703.998 kb on - strand at 2703.998 kb on - strand at 2704.155 kb on - strand at 2704.165 kb on - strand at 2704.210 kb on + strand at 2704.222 kb on + strand at 2704.223 kb on - strand at 2704.237 kb on - strand at 2704.244 kb on + strand at 2704.277 kb on + strand at 2704.278 kb on - strand at 2704.300 kb on + strand at 2704.406 kb on + strand, within Pf1N1B4_2386 at 2704.406 kb on + strand, within Pf1N1B4_2386 at 2704.406 kb on + strand, within Pf1N1B4_2386 at 2704.406 kb on + strand, within Pf1N1B4_2386 at 2704.406 kb on + strand, within Pf1N1B4_2386 at 2704.406 kb on + strand, within Pf1N1B4_2386 at 2704.406 kb on + strand, within Pf1N1B4_2386 at 2704.407 kb on - strand, within Pf1N1B4_2386 at 2704.407 kb on - strand, within Pf1N1B4_2386 at 2704.407 kb on - strand, within Pf1N1B4_2386 at 2704.407 kb on - strand, within Pf1N1B4_2386 at 2704.448 kb on + strand, within Pf1N1B4_2386 at 2704.448 kb on + strand, within Pf1N1B4_2386 at 2704.448 kb on + strand, within Pf1N1B4_2386 at 2704.449 kb on - strand, within Pf1N1B4_2386 at 2704.449 kb on - strand, within Pf1N1B4_2386 at 2704.588 kb on + strand, within Pf1N1B4_2386 at 2704.664 kb on + strand, within Pf1N1B4_2386 at 2704.664 kb on + strand, within Pf1N1B4_2386 at 2704.840 kb on + strand at 2704.877 kb on + strand at 2704.878 kb on - strand at 2704.882 kb on - strand at 2704.910 kb on + strand at 2704.910 kb on + strand at 2704.910 kb on + strand at 2704.911 kb on - strand at 2704.911 kb on - strand at 2705.027 kb on + strand at 2705.028 kb on - strand, within Pf1N1B4_2387 at 2705.245 kb on + strand, within Pf1N1B4_2387 at 2705.246 kb on - strand, within Pf1N1B4_2387 at 2705.267 kb on + strand, within Pf1N1B4_2387 at 2705.268 kb on - strand, within Pf1N1B4_2387 at 2705.349 kb on + strand, within Pf1N1B4_2387 at 2705.349 kb on + strand, within Pf1N1B4_2387 at 2705.349 kb on + strand, within Pf1N1B4_2387 at 2705.349 kb on + strand, within Pf1N1B4_2387 at 2705.349 kb on + strand, within Pf1N1B4_2387 at 2705.349 kb on + strand, within Pf1N1B4_2387 at 2705.349 kb on + strand, within Pf1N1B4_2387 at 2705.350 kb on - strand, within Pf1N1B4_2387 at 2705.350 kb on - strand, within Pf1N1B4_2387 at 2705.350 kb on - strand, within Pf1N1B4_2387 at 2705.350 kb on - strand, within Pf1N1B4_2387 at 2705.544 kb on + strand, within Pf1N1B4_2387 at 2705.544 kb on + strand, within Pf1N1B4_2387 at 2705.544 kb on + strand, within Pf1N1B4_2387 at 2705.545 kb on - strand, within Pf1N1B4_2387 at 2705.545 kb on - strand, within Pf1N1B4_2387 at 2705.545 kb on - strand, within Pf1N1B4_2387 at 2705.601 kb on + strand, within Pf1N1B4_2387 at 2705.601 kb on + strand, within Pf1N1B4_2387 at 2705.601 kb on + strand, within Pf1N1B4_2387 at 2705.602 kb on - strand, within Pf1N1B4_2387 at 2705.602 kb on - strand, within Pf1N1B4_2387 at 2705.647 kb on + strand, within Pf1N1B4_2387 at 2705.647 kb on + strand, within Pf1N1B4_2387 at 2705.648 kb on - strand, within Pf1N1B4_2387 at 2705.693 kb on + strand, within Pf1N1B4_2387 at 2705.693 kb on + strand, within Pf1N1B4_2387 at 2705.693 kb on + strand, within Pf1N1B4_2387 at 2705.694 kb on - strand, within Pf1N1B4_2387
Per-strain Table
Position Strand Gene LocusTag Fraction nitrite 10 mM remove 2,703,993 - -1.2 2,703,993 - +1.0 2,703,998 - -0.7 2,703,998 - -0.2 2,703,998 - -2.4 2,703,998 - -1.5 2,704,155 - -1.5 2,704,165 - -0.2 2,704,210 + -0.1 2,704,222 + -0.9 2,704,223 - -0.5 2,704,237 - -2.1 2,704,244 + +0.1 2,704,277 + -0.4 2,704,278 - -0.5 2,704,300 + +0.7 2,704,406 + Pf1N1B4_2386 0.26 +0.8 2,704,406 + Pf1N1B4_2386 0.26 -1.2 2,704,406 + Pf1N1B4_2386 0.26 -0.9 2,704,406 + Pf1N1B4_2386 0.26 -0.8 2,704,406 + Pf1N1B4_2386 0.26 -2.1 2,704,406 + Pf1N1B4_2386 0.26 -1.2 2,704,406 + Pf1N1B4_2386 0.26 -0.9 2,704,407 - Pf1N1B4_2386 0.26 -3.4 2,704,407 - Pf1N1B4_2386 0.26 -1.9 2,704,407 - Pf1N1B4_2386 0.26 -2.2 2,704,407 - Pf1N1B4_2386 0.26 -2.4 2,704,448 + Pf1N1B4_2386 0.36 -0.9 2,704,448 + Pf1N1B4_2386 0.36 -1.9 2,704,448 + Pf1N1B4_2386 0.36 -2.7 2,704,449 - Pf1N1B4_2386 0.37 +0.1 2,704,449 - Pf1N1B4_2386 0.37 -2.1 2,704,588 + Pf1N1B4_2386 0.71 -1.2 2,704,664 + Pf1N1B4_2386 0.90 -2.9 2,704,664 + Pf1N1B4_2386 0.90 -0.5 2,704,840 + +1.2 2,704,877 + +1.1 2,704,878 - -0.9 2,704,882 - +0.8 2,704,910 + +1.1 2,704,910 + -0.3 2,704,910 + +0.5 2,704,911 - -0.5 2,704,911 - -1.2 2,705,027 + +2.1 2,705,028 - Pf1N1B4_2387 0.13 +0.9 2,705,245 + Pf1N1B4_2387 0.38 +0.9 2,705,246 - Pf1N1B4_2387 0.38 -0.5 2,705,267 + Pf1N1B4_2387 0.40 -1.2 2,705,268 - Pf1N1B4_2387 0.40 +1.1 2,705,349 + Pf1N1B4_2387 0.49 +0.5 2,705,349 + Pf1N1B4_2387 0.49 +0.5 2,705,349 + Pf1N1B4_2387 0.49 -0.5 2,705,349 + Pf1N1B4_2387 0.49 +2.1 2,705,349 + Pf1N1B4_2387 0.49 -0.9 2,705,349 + Pf1N1B4_2387 0.49 -0.1 2,705,349 + Pf1N1B4_2387 0.49 +0.8 2,705,350 - Pf1N1B4_2387 0.49 +1.3 2,705,350 - Pf1N1B4_2387 0.49 +0.8 2,705,350 - Pf1N1B4_2387 0.49 +0.1 2,705,350 - Pf1N1B4_2387 0.49 +0.1 2,705,544 + Pf1N1B4_2387 0.71 -1.2 2,705,544 + Pf1N1B4_2387 0.71 +2.1 2,705,544 + Pf1N1B4_2387 0.71 +0.1 2,705,545 - Pf1N1B4_2387 0.71 -0.7 2,705,545 - Pf1N1B4_2387 0.71 -0.2 2,705,545 - Pf1N1B4_2387 0.71 +0.3 2,705,601 + Pf1N1B4_2387 0.78 +0.4 2,705,601 + Pf1N1B4_2387 0.78 +1.3 2,705,601 + Pf1N1B4_2387 0.78 +0.8 2,705,602 - Pf1N1B4_2387 0.78 -0.4 2,705,602 - Pf1N1B4_2387 0.78 -0.1 2,705,647 + Pf1N1B4_2387 0.83 +0.7 2,705,647 + Pf1N1B4_2387 0.83 -1.2 2,705,648 - Pf1N1B4_2387 0.83 +1.7 2,705,693 + Pf1N1B4_2387 0.88 +1.4 2,705,693 + Pf1N1B4_2387 0.88 +0.4 2,705,693 + Pf1N1B4_2387 0.88 +1.5 2,705,694 - Pf1N1B4_2387 0.88 -1.4
Or see this region's nucleotide sequence