Experiment: nitrite 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2243 and Pf1N1B4_2244 are separated by 167 nucleotides Pf1N1B4_2244 and Pf1N1B4_2245 are separated by 369 nucleotides
Pf1N1B4_2243 - N-acetylglutamate synthase (EC 2.3.1.1), at 2,558,421 to 2,559,719
_2243
Pf1N1B4_2244 - Taurine-binding periplasmic protein TauA, at 2,559,887 to 2,560,873
_2244
Pf1N1B4_2245 - Outer membrane porin, OprD family, at 2,561,243 to 2,562,601
_2245
Position (kb)
2559
2560
2561 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2559.803 kb on + strand at 2559.889 kb on - strand at 2559.889 kb on - strand at 2559.916 kb on - strand at 2560.144 kb on + strand, within Pf1N1B4_2244 at 2560.156 kb on - strand, within Pf1N1B4_2244 at 2560.455 kb on + strand, within Pf1N1B4_2244 at 2560.456 kb on - strand, within Pf1N1B4_2244 at 2560.456 kb on - strand, within Pf1N1B4_2244 at 2560.852 kb on + strand at 2560.853 kb on - strand at 2560.857 kb on - strand at 2560.907 kb on - strand at 2560.968 kb on - strand at 2560.983 kb on - strand at 2561.122 kb on - strand at 2561.332 kb on + strand at 2561.332 kb on + strand at 2561.332 kb on + strand at 2561.332 kb on + strand at 2561.333 kb on - strand at 2561.333 kb on - strand at 2561.333 kb on - strand at 2561.333 kb on - strand at 2561.333 kb on - strand at 2561.333 kb on - strand at 2561.347 kb on + strand at 2561.348 kb on - strand at 2561.354 kb on + strand at 2561.354 kb on + strand at 2561.354 kb on + strand at 2561.355 kb on - strand at 2561.355 kb on - strand at 2561.355 kb on - strand at 2561.355 kb on - strand at 2561.355 kb on - strand at 2561.457 kb on - strand, within Pf1N1B4_2245 at 2561.548 kb on + strand, within Pf1N1B4_2245 at 2561.548 kb on + strand, within Pf1N1B4_2245 at 2561.548 kb on + strand, within Pf1N1B4_2245 at 2561.548 kb on + strand, within Pf1N1B4_2245 at 2561.548 kb on + strand, within Pf1N1B4_2245 at 2561.549 kb on - strand, within Pf1N1B4_2245 at 2561.555 kb on + strand, within Pf1N1B4_2245 at 2561.556 kb on - strand, within Pf1N1B4_2245 at 2561.556 kb on - strand, within Pf1N1B4_2245 at 2561.568 kb on + strand, within Pf1N1B4_2245 at 2561.568 kb on + strand, within Pf1N1B4_2245 at 2561.568 kb on + strand, within Pf1N1B4_2245 at 2561.569 kb on - strand, within Pf1N1B4_2245 at 2561.569 kb on - strand at 2561.569 kb on - strand, within Pf1N1B4_2245 at 2561.569 kb on - strand, within Pf1N1B4_2245 at 2561.569 kb on - strand, within Pf1N1B4_2245 at 2561.602 kb on + strand, within Pf1N1B4_2245 at 2561.602 kb on + strand, within Pf1N1B4_2245 at 2561.602 kb on + strand, within Pf1N1B4_2245 at 2561.807 kb on - strand, within Pf1N1B4_2245
Per-strain Table
Position Strand Gene LocusTag Fraction nitrite 10 mM remove 2,559,803 + +0.8 2,559,889 - +0.7 2,559,889 - +0.7 2,559,916 - +0.6 2,560,144 + Pf1N1B4_2244 0.26 -0.1 2,560,156 - Pf1N1B4_2244 0.27 -1.2 2,560,455 + Pf1N1B4_2244 0.58 -2.1 2,560,456 - Pf1N1B4_2244 0.58 -0.9 2,560,456 - Pf1N1B4_2244 0.58 -2.1 2,560,852 + +0.0 2,560,853 - -0.2 2,560,857 - +1.3 2,560,907 - +1.1 2,560,968 - -0.5 2,560,983 - -0.3 2,561,122 - +1.5 2,561,332 + -0.2 2,561,332 + +1.1 2,561,332 + -0.1 2,561,332 + -0.1 2,561,333 - -0.6 2,561,333 - -0.3 2,561,333 - +0.1 2,561,333 - +0.8 2,561,333 - -0.2 2,561,333 - -0.8 2,561,347 + +0.9 2,561,348 - -0.5 2,561,354 + -0.7 2,561,354 + -0.9 2,561,354 + -0.2 2,561,355 - -1.5 2,561,355 - +1.8 2,561,355 - -0.5 2,561,355 - +1.5 2,561,355 - -0.7 2,561,457 - Pf1N1B4_2245 0.16 -0.2 2,561,548 + Pf1N1B4_2245 0.22 +0.3 2,561,548 + Pf1N1B4_2245 0.22 -0.9 2,561,548 + Pf1N1B4_2245 0.22 -0.1 2,561,548 + Pf1N1B4_2245 0.22 -2.6 2,561,548 + Pf1N1B4_2245 0.22 +0.1 2,561,549 - Pf1N1B4_2245 0.23 +0.4 2,561,555 + Pf1N1B4_2245 0.23 +0.1 2,561,556 - Pf1N1B4_2245 0.23 -0.2 2,561,556 - Pf1N1B4_2245 0.23 +0.1 2,561,568 + Pf1N1B4_2245 0.24 -0.7 2,561,568 + Pf1N1B4_2245 0.24 -0.5 2,561,568 + Pf1N1B4_2245 0.24 +0.1 2,561,569 - Pf1N1B4_2245 0.24 -0.1 2,561,569 - -0.5 2,561,569 - Pf1N1B4_2245 0.24 +1.1 2,561,569 - Pf1N1B4_2245 0.24 +0.8 2,561,569 - Pf1N1B4_2245 0.24 -2.1 2,561,602 + Pf1N1B4_2245 0.26 -0.2 2,561,602 + Pf1N1B4_2245 0.26 -0.9 2,561,602 + Pf1N1B4_2245 0.26 -1.0 2,561,807 - Pf1N1B4_2245 0.42 +0.7
Or see this region's nucleotide sequence