Experiment: nitrite 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2231 and Pf1N1B4_2232 are separated by 245 nucleotides Pf1N1B4_2232 and Pf1N1B4_2233 are separated by 210 nucleotides
Pf1N1B4_2231 - 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase (EC 1.14.13.-), at 2,542,627 to 2,543,850
_2231
Pf1N1B4_2232 - Ferric iron ABC transporter, iron-binding protein, at 2,544,096 to 2,545,100
_2232
Pf1N1B4_2233 - Ferric iron ABC transporter, permease protein, at 2,545,311 to 2,546,894
_2233
Position (kb)
2544
2545
2546 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2543.897 kb on + strand at 2543.897 kb on + strand at 2543.897 kb on + strand at 2543.937 kb on + strand at 2543.937 kb on + strand at 2543.938 kb on - strand at 2543.938 kb on - strand at 2543.938 kb on - strand at 2543.943 kb on + strand at 2543.943 kb on + strand at 2543.944 kb on - strand at 2543.944 kb on - strand at 2543.974 kb on + strand at 2543.974 kb on + strand at 2543.975 kb on - strand at 2544.115 kb on + strand at 2544.115 kb on + strand at 2544.183 kb on + strand at 2544.228 kb on + strand, within Pf1N1B4_2232 at 2544.228 kb on + strand, within Pf1N1B4_2232 at 2544.229 kb on - strand, within Pf1N1B4_2232 at 2544.229 kb on - strand, within Pf1N1B4_2232 at 2544.429 kb on + strand, within Pf1N1B4_2232 at 2544.486 kb on + strand, within Pf1N1B4_2232 at 2544.487 kb on - strand, within Pf1N1B4_2232 at 2544.525 kb on + strand, within Pf1N1B4_2232 at 2544.525 kb on + strand, within Pf1N1B4_2232 at 2544.526 kb on - strand, within Pf1N1B4_2232 at 2544.526 kb on - strand, within Pf1N1B4_2232 at 2544.588 kb on + strand, within Pf1N1B4_2232 at 2544.756 kb on + strand, within Pf1N1B4_2232 at 2544.756 kb on + strand, within Pf1N1B4_2232 at 2544.756 kb on + strand, within Pf1N1B4_2232 at 2544.756 kb on + strand, within Pf1N1B4_2232 at 2544.756 kb on + strand, within Pf1N1B4_2232 at 2544.757 kb on - strand, within Pf1N1B4_2232 at 2544.757 kb on - strand, within Pf1N1B4_2232 at 2544.757 kb on - strand, within Pf1N1B4_2232 at 2544.757 kb on - strand, within Pf1N1B4_2232 at 2544.757 kb on - strand, within Pf1N1B4_2232 at 2544.759 kb on + strand at 2544.997 kb on + strand, within Pf1N1B4_2232 at 2545.031 kb on - strand at 2545.112 kb on + strand at 2545.112 kb on + strand at 2545.112 kb on + strand at 2545.112 kb on + strand at 2545.112 kb on + strand at 2545.112 kb on + strand at 2545.112 kb on + strand at 2545.112 kb on + strand at 2545.112 kb on + strand at 2545.113 kb on - strand at 2545.113 kb on - strand at 2545.113 kb on - strand at 2545.113 kb on - strand at 2545.113 kb on - strand at 2545.113 kb on - strand at 2545.113 kb on - strand at 2545.262 kb on + strand at 2545.575 kb on + strand, within Pf1N1B4_2233 at 2545.575 kb on + strand, within Pf1N1B4_2233 at 2545.575 kb on + strand, within Pf1N1B4_2233 at 2545.575 kb on + strand at 2545.575 kb on + strand at 2545.576 kb on - strand, within Pf1N1B4_2233 at 2545.576 kb on - strand, within Pf1N1B4_2233 at 2545.576 kb on - strand, within Pf1N1B4_2233 at 2545.576 kb on - strand, within Pf1N1B4_2233 at 2545.576 kb on - strand, within Pf1N1B4_2233 at 2545.576 kb on - strand, within Pf1N1B4_2233 at 2545.576 kb on - strand, within Pf1N1B4_2233 at 2545.576 kb on - strand, within Pf1N1B4_2233 at 2545.576 kb on - strand, within Pf1N1B4_2233 at 2545.731 kb on + strand, within Pf1N1B4_2233 at 2545.732 kb on - strand, within Pf1N1B4_2233 at 2545.732 kb on - strand, within Pf1N1B4_2233 at 2545.732 kb on - strand, within Pf1N1B4_2233 at 2545.928 kb on + strand, within Pf1N1B4_2233 at 2545.947 kb on + strand at 2545.948 kb on - strand, within Pf1N1B4_2233 at 2546.032 kb on - strand, within Pf1N1B4_2233 at 2546.032 kb on - strand, within Pf1N1B4_2233 at 2546.097 kb on + strand, within Pf1N1B4_2233 at 2546.097 kb on + strand, within Pf1N1B4_2233
Per-strain Table
Position Strand Gene LocusTag Fraction nitrite 10 mM remove 2,543,897 + +0.5 2,543,897 + +1.2 2,543,897 + +1.8 2,543,937 + +1.1 2,543,937 + -1.2 2,543,938 - -0.8 2,543,938 - -0.9 2,543,938 - -0.1 2,543,943 + +0.6 2,543,943 + -0.8 2,543,944 - +1.1 2,543,944 - +1.8 2,543,974 + +0.4 2,543,974 + +0.1 2,543,975 - +0.3 2,544,115 + -0.1 2,544,115 + -1.5 2,544,183 + +0.2 2,544,228 + Pf1N1B4_2232 0.13 -0.5 2,544,228 + Pf1N1B4_2232 0.13 -1.2 2,544,229 - Pf1N1B4_2232 0.13 -0.9 2,544,229 - Pf1N1B4_2232 0.13 -0.9 2,544,429 + Pf1N1B4_2232 0.33 +0.2 2,544,486 + Pf1N1B4_2232 0.39 +1.1 2,544,487 - Pf1N1B4_2232 0.39 +0.4 2,544,525 + Pf1N1B4_2232 0.43 -0.3 2,544,525 + Pf1N1B4_2232 0.43 -0.1 2,544,526 - Pf1N1B4_2232 0.43 -1.1 2,544,526 - Pf1N1B4_2232 0.43 -2.1 2,544,588 + Pf1N1B4_2232 0.49 -0.7 2,544,756 + Pf1N1B4_2232 0.66 -1.2 2,544,756 + Pf1N1B4_2232 0.66 -0.9 2,544,756 + Pf1N1B4_2232 0.66 -0.7 2,544,756 + Pf1N1B4_2232 0.66 -1.5 2,544,756 + Pf1N1B4_2232 0.66 +0.6 2,544,757 - Pf1N1B4_2232 0.66 -1.1 2,544,757 - Pf1N1B4_2232 0.66 -2.8 2,544,757 - Pf1N1B4_2232 0.66 +1.1 2,544,757 - Pf1N1B4_2232 0.66 -1.2 2,544,757 - Pf1N1B4_2232 0.66 -1.2 2,544,759 + -0.9 2,544,997 + Pf1N1B4_2232 0.90 -1.9 2,545,031 - +0.1 2,545,112 + +0.5 2,545,112 + +1.6 2,545,112 + +1.1 2,545,112 + -0.1 2,545,112 + -0.5 2,545,112 + -1.2 2,545,112 + +0.4 2,545,112 + +0.8 2,545,112 + +0.8 2,545,113 - +1.4 2,545,113 - +0.1 2,545,113 - -0.9 2,545,113 - -0.5 2,545,113 - -2.2 2,545,113 - +0.5 2,545,113 - -0.8 2,545,262 + +0.1 2,545,575 + Pf1N1B4_2233 0.17 +0.1 2,545,575 + Pf1N1B4_2233 0.17 -0.2 2,545,575 + Pf1N1B4_2233 0.17 +0.7 2,545,575 + +0.7 2,545,575 + +1.1 2,545,576 - Pf1N1B4_2233 0.17 +1.1 2,545,576 - Pf1N1B4_2233 0.17 +0.3 2,545,576 - Pf1N1B4_2233 0.17 +0.9 2,545,576 - Pf1N1B4_2233 0.17 -0.5 2,545,576 - Pf1N1B4_2233 0.17 +0.1 2,545,576 - Pf1N1B4_2233 0.17 -0.3 2,545,576 - Pf1N1B4_2233 0.17 +0.1 2,545,576 - Pf1N1B4_2233 0.17 -0.8 2,545,576 - Pf1N1B4_2233 0.17 +0.6 2,545,731 + Pf1N1B4_2233 0.27 +0.5 2,545,732 - Pf1N1B4_2233 0.27 +0.1 2,545,732 - Pf1N1B4_2233 0.27 -0.8 2,545,732 - Pf1N1B4_2233 0.27 +0.2 2,545,928 + Pf1N1B4_2233 0.39 -1.5 2,545,947 + +1.1 2,545,948 - Pf1N1B4_2233 0.40 +0.6 2,546,032 - Pf1N1B4_2233 0.46 +1.9 2,546,032 - Pf1N1B4_2233 0.46 -0.5 2,546,097 + Pf1N1B4_2233 0.50 -1.0 2,546,097 + Pf1N1B4_2233 0.50 -1.4
Or see this region's nucleotide sequence