Experiment: nitrite 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2225 and Pf1N1B4_2226 are separated by 294 nucleotides Pf1N1B4_2226 and Pf1N1B4_2227 overlap by 4 nucleotides Pf1N1B4_2227 and Pf1N1B4_2228 are separated by 126 nucleotides Pf1N1B4_2228 and Pf1N1B4_2229 are separated by 27 nucleotides
Pf1N1B4_2225 - 5-formyltetrahydrofolate cyclo-ligase (EC 6.3.3.2), at 2,537,856 to 2,538,461
_2225
Pf1N1B4_2226 - Z-ring-associated protein ZapA, at 2,538,756 to 2,539,073
_2226
Pf1N1B4_2227 - FIG019278: hypothetical protein, at 2,539,070 to 2,539,279
_2227
Pf1N1B4_2228 - FIG001590: Putative conserved exported protein precursor, at 2,539,406 to 2,539,960
_2228
Pf1N1B4_2229 - Xaa-Pro aminopeptidase (EC 3.4.11.9), at 2,539,988 to 2,541,322
_2229
Position (kb)
2539
2540 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2538.487 kb on - strand at 2538.524 kb on + strand at 2538.524 kb on + strand at 2538.524 kb on + strand at 2538.525 kb on - strand at 2538.525 kb on - strand at 2538.545 kb on + strand at 2538.545 kb on + strand at 2538.545 kb on + strand at 2538.545 kb on + strand at 2538.545 kb on + strand at 2538.545 kb on + strand at 2538.546 kb on - strand at 2538.546 kb on - strand at 2538.575 kb on + strand at 2538.576 kb on - strand at 2538.613 kb on + strand at 2538.613 kb on + strand at 2538.614 kb on - strand at 2538.614 kb on - strand at 2538.635 kb on - strand at 2538.733 kb on + strand at 2538.733 kb on + strand at 2538.733 kb on + strand at 2538.734 kb on - strand at 2538.734 kb on - strand at 2538.734 kb on - strand at 2538.734 kb on - strand at 2538.734 kb on - strand at 2538.734 kb on - strand at 2538.734 kb on - strand at 2538.735 kb on + strand at 2538.735 kb on + strand at 2538.736 kb on - strand at 2539.188 kb on - strand, within Pf1N1B4_2227 at 2539.188 kb on - strand at 2539.191 kb on - strand, within Pf1N1B4_2227 at 2539.191 kb on - strand, within Pf1N1B4_2227 at 2539.191 kb on - strand, within Pf1N1B4_2227 at 2539.191 kb on - strand, within Pf1N1B4_2227 at 2539.212 kb on - strand, within Pf1N1B4_2227 at 2539.212 kb on - strand, within Pf1N1B4_2227 at 2539.310 kb on - strand at 2539.310 kb on - strand at 2539.310 kb on - strand at 2539.310 kb on - strand at 2539.310 kb on - strand at 2539.310 kb on - strand at 2539.310 kb on - strand at 2539.328 kb on - strand at 2539.328 kb on - strand at 2539.328 kb on - strand at 2539.328 kb on - strand at 2539.331 kb on - strand at 2539.331 kb on - strand at 2539.331 kb on - strand at 2539.331 kb on - strand at 2539.331 kb on - strand at 2539.331 kb on - strand at 2539.353 kb on + strand at 2539.354 kb on - strand at 2539.354 kb on - strand at 2539.354 kb on - strand at 2539.354 kb on - strand at 2539.354 kb on - strand at 2539.354 kb on - strand at 2539.354 kb on - strand at 2539.359 kb on - strand at 2539.359 kb on - strand at 2539.359 kb on - strand at 2539.359 kb on - strand at 2539.359 kb on - strand at 2539.359 kb on - strand at 2539.359 kb on - strand at 2539.359 kb on - strand at 2539.359 kb on - strand at 2539.359 kb on - strand at 2539.375 kb on - strand at 2539.375 kb on - strand at 2539.375 kb on - strand at 2539.375 kb on - strand at 2539.375 kb on - strand at 2539.381 kb on - strand at 2539.381 kb on - strand at 2539.381 kb on - strand at 2539.398 kb on - strand at 2539.398 kb on - strand at 2539.636 kb on + strand, within Pf1N1B4_2228 at 2539.637 kb on - strand, within Pf1N1B4_2228 at 2539.637 kb on - strand, within Pf1N1B4_2228 at 2539.763 kb on + strand, within Pf1N1B4_2228 at 2539.784 kb on - strand, within Pf1N1B4_2228 at 2539.862 kb on + strand at 2539.863 kb on - strand, within Pf1N1B4_2228 at 2539.863 kb on - strand, within Pf1N1B4_2228 at 2539.863 kb on - strand, within Pf1N1B4_2228 at 2539.880 kb on + strand, within Pf1N1B4_2228 at 2539.880 kb on + strand, within Pf1N1B4_2228 at 2539.881 kb on - strand, within Pf1N1B4_2228 at 2539.881 kb on - strand, within Pf1N1B4_2228 at 2539.881 kb on - strand, within Pf1N1B4_2228 at 2539.881 kb on - strand, within Pf1N1B4_2228 at 2539.958 kb on + strand at 2539.996 kb on + strand at 2540.012 kb on + strand at 2540.012 kb on + strand at 2540.141 kb on + strand, within Pf1N1B4_2229 at 2540.141 kb on + strand, within Pf1N1B4_2229 at 2540.142 kb on - strand, within Pf1N1B4_2229 at 2540.207 kb on + strand, within Pf1N1B4_2229 at 2540.207 kb on + strand, within Pf1N1B4_2229 at 2540.207 kb on + strand, within Pf1N1B4_2229 at 2540.207 kb on + strand, within Pf1N1B4_2229 at 2540.208 kb on - strand, within Pf1N1B4_2229 at 2540.208 kb on - strand, within Pf1N1B4_2229 at 2540.231 kb on - strand, within Pf1N1B4_2229 at 2540.369 kb on + strand, within Pf1N1B4_2229 at 2540.369 kb on + strand, within Pf1N1B4_2229 at 2540.370 kb on - strand, within Pf1N1B4_2229 at 2540.370 kb on - strand, within Pf1N1B4_2229 at 2540.471 kb on + strand, within Pf1N1B4_2229 at 2540.471 kb on + strand, within Pf1N1B4_2229 at 2540.471 kb on + strand, within Pf1N1B4_2229 at 2540.471 kb on + strand, within Pf1N1B4_2229 at 2540.471 kb on + strand, within Pf1N1B4_2229 at 2540.471 kb on + strand, within Pf1N1B4_2229 at 2540.471 kb on + strand, within Pf1N1B4_2229 at 2540.471 kb on + strand, within Pf1N1B4_2229 at 2540.471 kb on + strand, within Pf1N1B4_2229 at 2540.472 kb on - strand, within Pf1N1B4_2229 at 2540.472 kb on - strand, within Pf1N1B4_2229 at 2540.472 kb on - strand, within Pf1N1B4_2229 at 2540.472 kb on - strand, within Pf1N1B4_2229 at 2540.510 kb on + strand, within Pf1N1B4_2229 at 2540.511 kb on - strand, within Pf1N1B4_2229 at 2540.511 kb on - strand, within Pf1N1B4_2229 at 2540.511 kb on - strand, within Pf1N1B4_2229 at 2540.511 kb on - strand, within Pf1N1B4_2229 at 2540.511 kb on - strand, within Pf1N1B4_2229 at 2540.511 kb on - strand, within Pf1N1B4_2229 at 2540.512 kb on + strand, within Pf1N1B4_2229 at 2540.512 kb on + strand, within Pf1N1B4_2229 at 2540.513 kb on - strand, within Pf1N1B4_2229 at 2540.513 kb on - strand, within Pf1N1B4_2229 at 2540.513 kb on - strand, within Pf1N1B4_2229 at 2540.513 kb on - strand, within Pf1N1B4_2229 at 2540.513 kb on - strand, within Pf1N1B4_2229 at 2540.513 kb on - strand, within Pf1N1B4_2229 at 2540.513 kb on - strand, within Pf1N1B4_2229 at 2540.513 kb on - strand, within Pf1N1B4_2229 at 2540.535 kb on + strand, within Pf1N1B4_2229 at 2540.535 kb on + strand, within Pf1N1B4_2229 at 2540.535 kb on + strand, within Pf1N1B4_2229 at 2540.535 kb on + strand, within Pf1N1B4_2229 at 2540.536 kb on - strand, within Pf1N1B4_2229 at 2540.536 kb on - strand, within Pf1N1B4_2229 at 2540.536 kb on - strand, within Pf1N1B4_2229 at 2540.536 kb on - strand, within Pf1N1B4_2229 at 2540.637 kb on - strand, within Pf1N1B4_2229 at 2540.672 kb on + strand, within Pf1N1B4_2229 at 2540.673 kb on - strand, within Pf1N1B4_2229 at 2540.673 kb on - strand, within Pf1N1B4_2229 at 2540.717 kb on + strand at 2540.717 kb on + strand, within Pf1N1B4_2229 at 2540.717 kb on + strand, within Pf1N1B4_2229 at 2540.789 kb on + strand, within Pf1N1B4_2229 at 2540.831 kb on + strand, within Pf1N1B4_2229 at 2540.831 kb on + strand, within Pf1N1B4_2229 at 2540.831 kb on + strand, within Pf1N1B4_2229 at 2540.831 kb on + strand, within Pf1N1B4_2229 at 2540.831 kb on + strand, within Pf1N1B4_2229 at 2540.831 kb on + strand, within Pf1N1B4_2229 at 2540.831 kb on + strand, within Pf1N1B4_2229 at 2540.831 kb on + strand, within Pf1N1B4_2229 at 2540.831 kb on + strand, within Pf1N1B4_2229 at 2540.831 kb on + strand, within Pf1N1B4_2229 at 2540.831 kb on + strand, within Pf1N1B4_2229 at 2540.831 kb on + strand, within Pf1N1B4_2229 at 2540.831 kb on + strand, within Pf1N1B4_2229 at 2540.831 kb on + strand, within Pf1N1B4_2229 at 2540.832 kb on - strand, within Pf1N1B4_2229 at 2540.832 kb on - strand, within Pf1N1B4_2229 at 2540.832 kb on - strand, within Pf1N1B4_2229 at 2540.832 kb on - strand, within Pf1N1B4_2229 at 2540.832 kb on - strand, within Pf1N1B4_2229 at 2540.832 kb on - strand, within Pf1N1B4_2229 at 2540.832 kb on - strand, within Pf1N1B4_2229 at 2540.832 kb on - strand, within Pf1N1B4_2229 at 2540.832 kb on - strand, within Pf1N1B4_2229 at 2540.832 kb on - strand at 2540.832 kb on - strand, within Pf1N1B4_2229 at 2540.832 kb on - strand, within Pf1N1B4_2229 at 2540.855 kb on + strand, within Pf1N1B4_2229 at 2540.855 kb on + strand, within Pf1N1B4_2229 at 2540.856 kb on - strand, within Pf1N1B4_2229 at 2540.856 kb on - strand, within Pf1N1B4_2229 at 2540.856 kb on - strand, within Pf1N1B4_2229
Per-strain Table
Position Strand Gene LocusTag Fraction nitrite 10 mM remove 2,538,487 - +2.1 2,538,524 + -0.5 2,538,524 + +0.6 2,538,524 + +0.3 2,538,525 - +0.1 2,538,525 - +1.1 2,538,545 + -0.5 2,538,545 + -1.2 2,538,545 + +0.3 2,538,545 + -0.1 2,538,545 + -0.7 2,538,545 + +0.4 2,538,546 - +0.8 2,538,546 - -0.0 2,538,575 + +0.4 2,538,576 - +1.5 2,538,613 + +0.8 2,538,613 + -0.3 2,538,614 - +0.3 2,538,614 - -0.1 2,538,635 - -1.1 2,538,733 + -0.7 2,538,733 + +0.1 2,538,733 + -0.5 2,538,734 - +0.1 2,538,734 - +0.6 2,538,734 - -0.2 2,538,734 - -0.9 2,538,734 - -0.5 2,538,734 - -2.2 2,538,734 - +0.3 2,538,735 + +1.4 2,538,735 + -3.1 2,538,736 - -0.3 2,539,188 - Pf1N1B4_2227 0.56 -0.5 2,539,188 - +1.7 2,539,191 - Pf1N1B4_2227 0.58 +0.1 2,539,191 - Pf1N1B4_2227 0.58 +2.7 2,539,191 - Pf1N1B4_2227 0.58 +1.9 2,539,191 - Pf1N1B4_2227 0.58 -0.7 2,539,212 - Pf1N1B4_2227 0.68 +1.3 2,539,212 - Pf1N1B4_2227 0.68 +1.3 2,539,310 - -0.1 2,539,310 - +0.6 2,539,310 - +2.1 2,539,310 - +1.1 2,539,310 - +0.2 2,539,310 - +0.3 2,539,310 - -1.1 2,539,328 - -0.2 2,539,328 - -1.5 2,539,328 - +2.1 2,539,328 - +1.8 2,539,331 - +0.5 2,539,331 - -0.7 2,539,331 - +1.7 2,539,331 - -0.2 2,539,331 - -0.5 2,539,331 - -1.2 2,539,353 + -2.1 2,539,354 - -0.2 2,539,354 - -0.5 2,539,354 - -2.1 2,539,354 - -1.7 2,539,354 - -1.9 2,539,354 - -0.5 2,539,354 - +0.1 2,539,359 - +1.1 2,539,359 - -0.5 2,539,359 - -2.9 2,539,359 - -0.4 2,539,359 - +0.3 2,539,359 - -1.5 2,539,359 - +0.8 2,539,359 - -1.2 2,539,359 - -1.5 2,539,359 - -1.2 2,539,375 - +1.1 2,539,375 - -1.3 2,539,375 - -1.9 2,539,375 - -0.9 2,539,375 - -1.5 2,539,381 - -1.2 2,539,381 - -1.7 2,539,381 - -2.5 2,539,398 - -1.7 2,539,398 - +0.3 2,539,636 + Pf1N1B4_2228 0.41 -0.7 2,539,637 - Pf1N1B4_2228 0.42 -0.5 2,539,637 - Pf1N1B4_2228 0.42 -0.1 2,539,763 + Pf1N1B4_2228 0.64 +0.2 2,539,784 - Pf1N1B4_2228 0.68 -0.9 2,539,862 + +0.1 2,539,863 - Pf1N1B4_2228 0.82 -1.6 2,539,863 - Pf1N1B4_2228 0.82 -1.1 2,539,863 - Pf1N1B4_2228 0.82 -0.3 2,539,880 + Pf1N1B4_2228 0.85 +0.7 2,539,880 + Pf1N1B4_2228 0.85 -0.5 2,539,881 - Pf1N1B4_2228 0.86 -2.1 2,539,881 - Pf1N1B4_2228 0.86 -0.5 2,539,881 - Pf1N1B4_2228 0.86 -0.1 2,539,881 - Pf1N1B4_2228 0.86 -1.7 2,539,958 + +0.3 2,539,996 + -0.6 2,540,012 + -1.9 2,540,012 + -0.2 2,540,141 + Pf1N1B4_2229 0.11 +1.3 2,540,141 + Pf1N1B4_2229 0.11 -0.4 2,540,142 - Pf1N1B4_2229 0.12 -0.5 2,540,207 + Pf1N1B4_2229 0.16 +0.6 2,540,207 + Pf1N1B4_2229 0.16 -0.3 2,540,207 + Pf1N1B4_2229 0.16 +0.7 2,540,207 + Pf1N1B4_2229 0.16 +0.6 2,540,208 - Pf1N1B4_2229 0.16 -1.1 2,540,208 - Pf1N1B4_2229 0.16 -1.7 2,540,231 - Pf1N1B4_2229 0.18 -0.2 2,540,369 + Pf1N1B4_2229 0.29 +1.3 2,540,369 + Pf1N1B4_2229 0.29 +0.1 2,540,370 - Pf1N1B4_2229 0.29 -0.3 2,540,370 - Pf1N1B4_2229 0.29 -1.1 2,540,471 + Pf1N1B4_2229 0.36 +1.0 2,540,471 + Pf1N1B4_2229 0.36 -0.1 2,540,471 + Pf1N1B4_2229 0.36 +0.3 2,540,471 + Pf1N1B4_2229 0.36 +0.3 2,540,471 + Pf1N1B4_2229 0.36 +2.1 2,540,471 + Pf1N1B4_2229 0.36 +0.1 2,540,471 + Pf1N1B4_2229 0.36 -0.3 2,540,471 + Pf1N1B4_2229 0.36 +0.2 2,540,471 + Pf1N1B4_2229 0.36 +0.9 2,540,472 - Pf1N1B4_2229 0.36 -0.9 2,540,472 - Pf1N1B4_2229 0.36 -1.2 2,540,472 - Pf1N1B4_2229 0.36 -3.2 2,540,472 - Pf1N1B4_2229 0.36 -1.4 2,540,510 + Pf1N1B4_2229 0.39 -0.8 2,540,511 - Pf1N1B4_2229 0.39 +1.9 2,540,511 - Pf1N1B4_2229 0.39 -0.5 2,540,511 - Pf1N1B4_2229 0.39 -1.7 2,540,511 - Pf1N1B4_2229 0.39 +0.7 2,540,511 - Pf1N1B4_2229 0.39 -1.1 2,540,511 - Pf1N1B4_2229 0.39 -0.9 2,540,512 + Pf1N1B4_2229 0.39 +0.2 2,540,512 + Pf1N1B4_2229 0.39 +1.4 2,540,513 - Pf1N1B4_2229 0.39 +0.8 2,540,513 - Pf1N1B4_2229 0.39 -0.2 2,540,513 - Pf1N1B4_2229 0.39 -1.7 2,540,513 - Pf1N1B4_2229 0.39 -2.4 2,540,513 - Pf1N1B4_2229 0.39 -0.5 2,540,513 - Pf1N1B4_2229 0.39 -2.1 2,540,513 - Pf1N1B4_2229 0.39 -2.6 2,540,513 - Pf1N1B4_2229 0.39 +0.3 2,540,535 + Pf1N1B4_2229 0.41 -0.7 2,540,535 + Pf1N1B4_2229 0.41 -1.2 2,540,535 + Pf1N1B4_2229 0.41 +0.8 2,540,535 + Pf1N1B4_2229 0.41 -0.0 2,540,536 - Pf1N1B4_2229 0.41 -0.9 2,540,536 - Pf1N1B4_2229 0.41 -1.7 2,540,536 - Pf1N1B4_2229 0.41 -1.1 2,540,536 - Pf1N1B4_2229 0.41 -0.9 2,540,637 - Pf1N1B4_2229 0.49 +0.1 2,540,672 + Pf1N1B4_2229 0.51 +0.1 2,540,673 - Pf1N1B4_2229 0.51 -0.2 2,540,673 - Pf1N1B4_2229 0.51 -2.2 2,540,717 + +1.1 2,540,717 + Pf1N1B4_2229 0.55 +1.2 2,540,717 + Pf1N1B4_2229 0.55 +0.3 2,540,789 + Pf1N1B4_2229 0.60 -0.3 2,540,831 + Pf1N1B4_2229 0.63 +0.1 2,540,831 + Pf1N1B4_2229 0.63 +0.5 2,540,831 + Pf1N1B4_2229 0.63 +0.1 2,540,831 + Pf1N1B4_2229 0.63 -0.1 2,540,831 + Pf1N1B4_2229 0.63 +0.7 2,540,831 + Pf1N1B4_2229 0.63 -0.5 2,540,831 + Pf1N1B4_2229 0.63 +0.4 2,540,831 + Pf1N1B4_2229 0.63 -1.1 2,540,831 + Pf1N1B4_2229 0.63 +1.9 2,540,831 + Pf1N1B4_2229 0.63 +0.3 2,540,831 + Pf1N1B4_2229 0.63 +0.5 2,540,831 + Pf1N1B4_2229 0.63 -0.6 2,540,831 + Pf1N1B4_2229 0.63 +0.6 2,540,831 + Pf1N1B4_2229 0.63 +1.1 2,540,832 - Pf1N1B4_2229 0.63 -1.2 2,540,832 - Pf1N1B4_2229 0.63 -2.5 2,540,832 - Pf1N1B4_2229 0.63 -1.2 2,540,832 - Pf1N1B4_2229 0.63 -1.7 2,540,832 - Pf1N1B4_2229 0.63 -1.2 2,540,832 - Pf1N1B4_2229 0.63 -1.7 2,540,832 - Pf1N1B4_2229 0.63 -0.2 2,540,832 - Pf1N1B4_2229 0.63 -0.9 2,540,832 - Pf1N1B4_2229 0.63 -1.9 2,540,832 - +2.1 2,540,832 - Pf1N1B4_2229 0.63 +0.1 2,540,832 - Pf1N1B4_2229 0.63 -1.2 2,540,855 + Pf1N1B4_2229 0.65 +0.6 2,540,855 + Pf1N1B4_2229 0.65 -0.1 2,540,856 - Pf1N1B4_2229 0.65 -0.5 2,540,856 - Pf1N1B4_2229 0.65 -0.9 2,540,856 - Pf1N1B4_2229 0.65 -0.9
Or see this region's nucleotide sequence