Experiment: nitrite 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1914 and Pf1N1B4_1915 are separated by 22 nucleotides Pf1N1B4_1915 and Pf1N1B4_1916 are separated by 131 nucleotides Pf1N1B4_1916 and Pf1N1B4_1917 are separated by 102 nucleotides
Pf1N1B4_1914 - Ribosomal RNA small subunit methyltransferase B (EC 2.1.1.-), at 2,188,260 to 2,189,570
_1914
Pf1N1B4_1915 - Trk system potassium uptake protein TrkA, at 2,189,593 to 2,190,966
_1915
Pf1N1B4_1916 - Chaperone protein YscY (Yop proteins translocation protein Y), at 2,191,098 to 2,191,412
_1916
Pf1N1B4_1917 - Lipid A biosynthesis lauroyl acyltransferase (EC 2.3.1.-), at 2,191,515 to 2,192,402
_1917
Position (kb)
2189
2190
2191 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2188.812 kb on + strand, within Pf1N1B4_1914 at 2188.813 kb on - strand, within Pf1N1B4_1914 at 2188.813 kb on - strand, within Pf1N1B4_1914 at 2188.813 kb on - strand, within Pf1N1B4_1914 at 2188.813 kb on - strand, within Pf1N1B4_1914 at 2188.813 kb on - strand, within Pf1N1B4_1914 at 2188.813 kb on - strand, within Pf1N1B4_1914 at 2188.860 kb on + strand, within Pf1N1B4_1914 at 2188.860 kb on + strand, within Pf1N1B4_1914 at 2188.861 kb on - strand, within Pf1N1B4_1914 at 2188.877 kb on + strand, within Pf1N1B4_1914 at 2188.877 kb on + strand, within Pf1N1B4_1914 at 2189.330 kb on + strand, within Pf1N1B4_1914 at 2189.331 kb on - strand, within Pf1N1B4_1914 at 2189.331 kb on - strand, within Pf1N1B4_1914 at 2189.331 kb on - strand, within Pf1N1B4_1914 at 2189.331 kb on - strand, within Pf1N1B4_1914 at 2189.331 kb on - strand, within Pf1N1B4_1914 at 2189.368 kb on - strand, within Pf1N1B4_1914 at 2189.497 kb on + strand at 2189.497 kb on + strand at 2189.529 kb on + strand at 2189.530 kb on - strand at 2189.532 kb on + strand at 2189.578 kb on + strand at 2189.579 kb on - strand at 2189.903 kb on - strand, within Pf1N1B4_1915 at 2189.983 kb on + strand, within Pf1N1B4_1915 at 2189.983 kb on + strand, within Pf1N1B4_1915 at 2189.983 kb on + strand, within Pf1N1B4_1915 at 2189.983 kb on + strand, within Pf1N1B4_1915 at 2189.983 kb on + strand, within Pf1N1B4_1915 at 2189.983 kb on + strand, within Pf1N1B4_1915 at 2189.983 kb on + strand, within Pf1N1B4_1915 at 2189.983 kb on + strand, within Pf1N1B4_1915 at 2189.983 kb on + strand, within Pf1N1B4_1915 at 2189.983 kb on + strand, within Pf1N1B4_1915 at 2189.983 kb on + strand, within Pf1N1B4_1915 at 2189.983 kb on + strand, within Pf1N1B4_1915 at 2189.983 kb on + strand, within Pf1N1B4_1915 at 2189.984 kb on - strand, within Pf1N1B4_1915 at 2189.984 kb on - strand, within Pf1N1B4_1915 at 2189.984 kb on - strand, within Pf1N1B4_1915 at 2189.984 kb on - strand, within Pf1N1B4_1915 at 2189.984 kb on - strand, within Pf1N1B4_1915 at 2189.984 kb on - strand, within Pf1N1B4_1915 at 2189.984 kb on - strand, within Pf1N1B4_1915 at 2189.984 kb on - strand, within Pf1N1B4_1915 at 2189.984 kb on - strand, within Pf1N1B4_1915 at 2189.984 kb on - strand, within Pf1N1B4_1915 at 2189.984 kb on - strand, within Pf1N1B4_1915 at 2189.984 kb on - strand, within Pf1N1B4_1915 at 2189.984 kb on - strand, within Pf1N1B4_1915 at 2189.984 kb on - strand, within Pf1N1B4_1915 at 2189.984 kb on - strand, within Pf1N1B4_1915 at 2189.984 kb on - strand, within Pf1N1B4_1915 at 2190.023 kb on + strand, within Pf1N1B4_1915 at 2190.023 kb on + strand, within Pf1N1B4_1915 at 2190.023 kb on + strand, within Pf1N1B4_1915 at 2190.023 kb on + strand, within Pf1N1B4_1915 at 2190.023 kb on + strand, within Pf1N1B4_1915 at 2190.024 kb on - strand, within Pf1N1B4_1915 at 2190.070 kb on + strand, within Pf1N1B4_1915 at 2190.070 kb on + strand, within Pf1N1B4_1915 at 2190.071 kb on - strand, within Pf1N1B4_1915 at 2190.071 kb on - strand, within Pf1N1B4_1915 at 2190.071 kb on - strand, within Pf1N1B4_1915 at 2190.071 kb on - strand, within Pf1N1B4_1915 at 2190.071 kb on - strand, within Pf1N1B4_1915 at 2190.074 kb on - strand, within Pf1N1B4_1915 at 2190.215 kb on + strand, within Pf1N1B4_1915 at 2190.215 kb on + strand, within Pf1N1B4_1915 at 2190.215 kb on + strand, within Pf1N1B4_1915 at 2190.240 kb on + strand, within Pf1N1B4_1915 at 2190.283 kb on + strand, within Pf1N1B4_1915 at 2190.283 kb on + strand, within Pf1N1B4_1915 at 2190.283 kb on + strand, within Pf1N1B4_1915 at 2190.283 kb on + strand, within Pf1N1B4_1915 at 2190.283 kb on + strand, within Pf1N1B4_1915 at 2190.283 kb on + strand, within Pf1N1B4_1915 at 2190.283 kb on + strand, within Pf1N1B4_1915 at 2190.283 kb on + strand, within Pf1N1B4_1915 at 2190.283 kb on + strand, within Pf1N1B4_1915 at 2190.283 kb on + strand at 2190.284 kb on - strand, within Pf1N1B4_1915 at 2190.284 kb on - strand, within Pf1N1B4_1915 at 2190.284 kb on - strand, within Pf1N1B4_1915 at 2190.284 kb on - strand, within Pf1N1B4_1915 at 2190.284 kb on - strand, within Pf1N1B4_1915 at 2190.284 kb on - strand, within Pf1N1B4_1915 at 2190.284 kb on - strand, within Pf1N1B4_1915 at 2190.284 kb on - strand, within Pf1N1B4_1915 at 2190.284 kb on - strand, within Pf1N1B4_1915 at 2190.284 kb on - strand, within Pf1N1B4_1915 at 2190.291 kb on + strand, within Pf1N1B4_1915 at 2190.291 kb on + strand, within Pf1N1B4_1915 at 2190.291 kb on + strand, within Pf1N1B4_1915 at 2190.291 kb on + strand, within Pf1N1B4_1915 at 2190.291 kb on + strand, within Pf1N1B4_1915 at 2190.292 kb on - strand, within Pf1N1B4_1915 at 2190.292 kb on - strand, within Pf1N1B4_1915 at 2190.292 kb on - strand, within Pf1N1B4_1915 at 2190.292 kb on - strand, within Pf1N1B4_1915 at 2190.292 kb on - strand, within Pf1N1B4_1915 at 2190.360 kb on - strand, within Pf1N1B4_1915 at 2190.592 kb on + strand, within Pf1N1B4_1915 at 2190.592 kb on + strand, within Pf1N1B4_1915 at 2190.593 kb on - strand, within Pf1N1B4_1915 at 2190.593 kb on - strand, within Pf1N1B4_1915 at 2190.593 kb on - strand, within Pf1N1B4_1915 at 2190.593 kb on - strand, within Pf1N1B4_1915 at 2190.909 kb on + strand at 2190.918 kb on + strand at 2190.918 kb on + strand at 2191.173 kb on + strand, within Pf1N1B4_1916 at 2191.242 kb on + strand, within Pf1N1B4_1916 at 2191.243 kb on - strand, within Pf1N1B4_1916 at 2191.243 kb on - strand, within Pf1N1B4_1916 at 2191.262 kb on - strand, within Pf1N1B4_1916 at 2191.407 kb on + strand at 2191.407 kb on + strand at 2191.407 kb on + strand at 2191.408 kb on - strand at 2191.408 kb on - strand at 2191.420 kb on + strand at 2191.420 kb on + strand at 2191.420 kb on + strand at 2191.420 kb on + strand at 2191.420 kb on + strand at 2191.420 kb on + strand at 2191.421 kb on - strand at 2191.421 kb on - strand at 2191.464 kb on + strand at 2191.520 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrite 10 mM remove 2,188,812 + Pf1N1B4_1914 0.42 -1.0 2,188,813 - Pf1N1B4_1914 0.42 -0.9 2,188,813 - Pf1N1B4_1914 0.42 -0.1 2,188,813 - Pf1N1B4_1914 0.42 +1.9 2,188,813 - Pf1N1B4_1914 0.42 -1.0 2,188,813 - Pf1N1B4_1914 0.42 +0.3 2,188,813 - Pf1N1B4_1914 0.42 -2.0 2,188,860 + Pf1N1B4_1914 0.46 +0.7 2,188,860 + Pf1N1B4_1914 0.46 -0.2 2,188,861 - Pf1N1B4_1914 0.46 +0.1 2,188,877 + Pf1N1B4_1914 0.47 +2.1 2,188,877 + Pf1N1B4_1914 0.47 +0.9 2,189,330 + Pf1N1B4_1914 0.82 -0.9 2,189,331 - Pf1N1B4_1914 0.82 -1.8 2,189,331 - Pf1N1B4_1914 0.82 -0.9 2,189,331 - Pf1N1B4_1914 0.82 +0.3 2,189,331 - Pf1N1B4_1914 0.82 -0.9 2,189,331 - Pf1N1B4_1914 0.82 -0.9 2,189,368 - Pf1N1B4_1914 0.85 +1.1 2,189,497 + -0.5 2,189,497 + +0.1 2,189,529 + +0.5 2,189,530 - -1.9 2,189,532 + +2.7 2,189,578 + -1.3 2,189,579 - -0.9 2,189,903 - Pf1N1B4_1915 0.23 +0.1 2,189,983 + Pf1N1B4_1915 0.28 -0.2 2,189,983 + Pf1N1B4_1915 0.28 +0.7 2,189,983 + Pf1N1B4_1915 0.28 -0.7 2,189,983 + Pf1N1B4_1915 0.28 -1.6 2,189,983 + Pf1N1B4_1915 0.28 -0.1 2,189,983 + Pf1N1B4_1915 0.28 -2.5 2,189,983 + Pf1N1B4_1915 0.28 -1.1 2,189,983 + Pf1N1B4_1915 0.28 +0.1 2,189,983 + Pf1N1B4_1915 0.28 +0.4 2,189,983 + Pf1N1B4_1915 0.28 -1.2 2,189,983 + Pf1N1B4_1915 0.28 -0.2 2,189,983 + Pf1N1B4_1915 0.28 -0.1 2,189,983 + Pf1N1B4_1915 0.28 -0.5 2,189,984 - Pf1N1B4_1915 0.28 +0.4 2,189,984 - Pf1N1B4_1915 0.28 -2.0 2,189,984 - Pf1N1B4_1915 0.28 -0.2 2,189,984 - Pf1N1B4_1915 0.28 -0.5 2,189,984 - Pf1N1B4_1915 0.28 +0.1 2,189,984 - Pf1N1B4_1915 0.28 -1.0 2,189,984 - Pf1N1B4_1915 0.28 -1.0 2,189,984 - Pf1N1B4_1915 0.28 +0.5 2,189,984 - Pf1N1B4_1915 0.28 +0.1 2,189,984 - Pf1N1B4_1915 0.28 -0.2 2,189,984 - Pf1N1B4_1915 0.28 -0.7 2,189,984 - Pf1N1B4_1915 0.28 -0.9 2,189,984 - Pf1N1B4_1915 0.28 -0.1 2,189,984 - Pf1N1B4_1915 0.28 -1.1 2,189,984 - Pf1N1B4_1915 0.28 +1.0 2,189,984 - Pf1N1B4_1915 0.28 -1.2 2,190,023 + Pf1N1B4_1915 0.31 +1.6 2,190,023 + Pf1N1B4_1915 0.31 -1.0 2,190,023 + Pf1N1B4_1915 0.31 -2.3 2,190,023 + Pf1N1B4_1915 0.31 -0.4 2,190,023 + Pf1N1B4_1915 0.31 -1.7 2,190,024 - Pf1N1B4_1915 0.31 +0.3 2,190,070 + Pf1N1B4_1915 0.35 -0.5 2,190,070 + Pf1N1B4_1915 0.35 +0.5 2,190,071 - Pf1N1B4_1915 0.35 +0.0 2,190,071 - Pf1N1B4_1915 0.35 -0.5 2,190,071 - Pf1N1B4_1915 0.35 -0.7 2,190,071 - Pf1N1B4_1915 0.35 -0.6 2,190,071 - Pf1N1B4_1915 0.35 -2.5 2,190,074 - Pf1N1B4_1915 0.35 +0.8 2,190,215 + Pf1N1B4_1915 0.45 -0.5 2,190,215 + Pf1N1B4_1915 0.45 +1.1 2,190,215 + Pf1N1B4_1915 0.45 -1.5 2,190,240 + Pf1N1B4_1915 0.47 +0.6 2,190,283 + Pf1N1B4_1915 0.50 -0.5 2,190,283 + Pf1N1B4_1915 0.50 +0.3 2,190,283 + Pf1N1B4_1915 0.50 -1.0 2,190,283 + Pf1N1B4_1915 0.50 +2.7 2,190,283 + Pf1N1B4_1915 0.50 +0.1 2,190,283 + Pf1N1B4_1915 0.50 -1.7 2,190,283 + Pf1N1B4_1915 0.50 -0.0 2,190,283 + Pf1N1B4_1915 0.50 -0.1 2,190,283 + Pf1N1B4_1915 0.50 +0.5 2,190,283 + +1.1 2,190,284 - Pf1N1B4_1915 0.50 -1.2 2,190,284 - Pf1N1B4_1915 0.50 -1.9 2,190,284 - Pf1N1B4_1915 0.50 -0.3 2,190,284 - Pf1N1B4_1915 0.50 -0.5 2,190,284 - Pf1N1B4_1915 0.50 -1.9 2,190,284 - Pf1N1B4_1915 0.50 -0.9 2,190,284 - Pf1N1B4_1915 0.50 -0.2 2,190,284 - Pf1N1B4_1915 0.50 +0.9 2,190,284 - Pf1N1B4_1915 0.50 -1.9 2,190,284 - Pf1N1B4_1915 0.50 -2.1 2,190,291 + Pf1N1B4_1915 0.51 -2.0 2,190,291 + Pf1N1B4_1915 0.51 -1.0 2,190,291 + Pf1N1B4_1915 0.51 -0.8 2,190,291 + Pf1N1B4_1915 0.51 -0.1 2,190,291 + Pf1N1B4_1915 0.51 +1.1 2,190,292 - Pf1N1B4_1915 0.51 -0.6 2,190,292 - Pf1N1B4_1915 0.51 -1.5 2,190,292 - Pf1N1B4_1915 0.51 -0.2 2,190,292 - Pf1N1B4_1915 0.51 +0.1 2,190,292 - Pf1N1B4_1915 0.51 -0.1 2,190,360 - Pf1N1B4_1915 0.56 +0.0 2,190,592 + Pf1N1B4_1915 0.73 -1.2 2,190,592 + Pf1N1B4_1915 0.73 -2.5 2,190,593 - Pf1N1B4_1915 0.73 -1.1 2,190,593 - Pf1N1B4_1915 0.73 +0.1 2,190,593 - Pf1N1B4_1915 0.73 +1.1 2,190,593 - Pf1N1B4_1915 0.73 -0.0 2,190,909 + +0.2 2,190,918 + -0.1 2,190,918 + -0.3 2,191,173 + Pf1N1B4_1916 0.24 +0.1 2,191,242 + Pf1N1B4_1916 0.46 -1.0 2,191,243 - Pf1N1B4_1916 0.46 -1.0 2,191,243 - Pf1N1B4_1916 0.46 +0.6 2,191,262 - Pf1N1B4_1916 0.52 -0.0 2,191,407 + -0.8 2,191,407 + +0.1 2,191,407 + -0.5 2,191,408 - -0.3 2,191,408 - +0.7 2,191,420 + -0.9 2,191,420 + -0.5 2,191,420 + +1.1 2,191,420 + -0.5 2,191,420 + -0.4 2,191,420 + -0.2 2,191,421 - -1.6 2,191,421 - -0.9 2,191,464 + -0.1 2,191,520 - -1.1
Or see this region's nucleotide sequence