Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_1236

Experiment: nitrite 10 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_1235 and Pf1N1B4_1236 overlap by 4 nucleotidesPf1N1B4_1236 and Pf1N1B4_5178 are separated by 154 nucleotidesPf1N1B4_5178 and Pf1N1B4_5179 are separated by 171 nucleotides Pf1N1B4_1235 - Exodeoxyribonuclease V beta chain (EC 3.1.11.5), at 1,402,162 to 1,405,851 _1235 Pf1N1B4_1236 - Exodeoxyribonuclease V gamma chain (EC 3.1.11.5), at 1,405,848 to 1,409,300 _1236 Pf1N1B4_5178 - 5S RNA, at 1,409,455 to 1,409,574 _5178 Pf1N1B4_5179 - Large Subunit Ribosomal RNA; lsuRNA; LSU rRNA, at 1,409,746 to 1,412,637 _5179 Position (kb) 1405 1406 1407 1408 1409 1410Strain fitness (log2 ratio) -2 -1 0 1 2at 1404.998 kb on - strand, within Pf1N1B4_1235at 1405.377 kb on + strand, within Pf1N1B4_1235at 1405.629 kb on + strandat 1405.749 kb on - strandat 1405.761 kb on + strandat 1405.761 kb on + strandat 1405.761 kb on + strandat 1406.030 kb on - strandat 1406.030 kb on - strandat 1406.098 kb on - strandat 1406.225 kb on + strand, within Pf1N1B4_1236at 1406.403 kb on + strand, within Pf1N1B4_1236at 1406.580 kb on + strand, within Pf1N1B4_1236at 1406.739 kb on + strand, within Pf1N1B4_1236at 1406.740 kb on - strand, within Pf1N1B4_1236at 1406.797 kb on + strand, within Pf1N1B4_1236at 1406.874 kb on + strand, within Pf1N1B4_1236at 1406.874 kb on + strand, within Pf1N1B4_1236at 1406.875 kb on - strand, within Pf1N1B4_1236at 1406.896 kb on + strand, within Pf1N1B4_1236at 1406.897 kb on - strand, within Pf1N1B4_1236at 1407.067 kb on + strand, within Pf1N1B4_1236at 1407.067 kb on + strand, within Pf1N1B4_1236at 1407.067 kb on + strand, within Pf1N1B4_1236at 1407.068 kb on - strand, within Pf1N1B4_1236at 1407.068 kb on - strand, within Pf1N1B4_1236at 1407.068 kb on - strand, within Pf1N1B4_1236at 1407.068 kb on - strand, within Pf1N1B4_1236at 1407.354 kb on + strand, within Pf1N1B4_1236at 1407.430 kb on + strandat 1407.463 kb on - strand, within Pf1N1B4_1236at 1407.546 kb on - strand, within Pf1N1B4_1236at 1407.799 kb on - strand, within Pf1N1B4_1236at 1408.006 kb on + strand, within Pf1N1B4_1236at 1408.006 kb on + strand, within Pf1N1B4_1236at 1408.006 kb on + strandat 1408.007 kb on - strand, within Pf1N1B4_1236at 1408.007 kb on - strand, within Pf1N1B4_1236at 1408.095 kb on + strandat 1408.096 kb on - strandat 1408.294 kb on + strand, within Pf1N1B4_1236at 1408.299 kb on - strand, within Pf1N1B4_1236at 1408.299 kb on - strand, within Pf1N1B4_1236at 1408.299 kb on - strandat 1408.328 kb on + strand, within Pf1N1B4_1236at 1408.328 kb on + strand, within Pf1N1B4_1236at 1408.334 kb on - strand, within Pf1N1B4_1236at 1408.442 kb on - strand, within Pf1N1B4_1236at 1408.880 kb on + strand, within Pf1N1B4_1236at 1408.880 kb on + strand, within Pf1N1B4_1236at 1408.885 kb on - strand, within Pf1N1B4_1236at 1408.885 kb on - strand, within Pf1N1B4_1236at 1408.885 kb on - strand, within Pf1N1B4_1236at 1409.167 kb on - strandat 1409.267 kb on + strandat 1409.267 kb on + strandat 1409.268 kb on - strandat 1409.268 kb on - strandat 1409.300 kb on + strandat 1409.301 kb on - strandat 1409.301 kb on - strandat 1409.301 kb on - strandat 1409.320 kb on + strandat 1409.330 kb on - strandat 1409.342 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrite 10 mM
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1,404,998 - Pf1N1B4_1235 0.77 -0.6
1,405,377 + Pf1N1B4_1235 0.87 +0.6
1,405,629 + -0.9
1,405,749 - -1.0
1,405,761 + -1.3
1,405,761 + -1.0
1,405,761 + +0.2
1,406,030 - +2.0
1,406,030 - -1.3
1,406,098 - +0.0
1,406,225 + Pf1N1B4_1236 0.11 +0.2
1,406,403 + Pf1N1B4_1236 0.16 -0.7
1,406,580 + Pf1N1B4_1236 0.21 -0.6
1,406,739 + Pf1N1B4_1236 0.26 -0.6
1,406,740 - Pf1N1B4_1236 0.26 -1.3
1,406,797 + Pf1N1B4_1236 0.27 -1.3
1,406,874 + Pf1N1B4_1236 0.30 +1.4
1,406,874 + Pf1N1B4_1236 0.30 -0.8
1,406,875 - Pf1N1B4_1236 0.30 -0.3
1,406,896 + Pf1N1B4_1236 0.30 -0.8
1,406,897 - Pf1N1B4_1236 0.30 -1.2
1,407,067 + Pf1N1B4_1236 0.35 -0.6
1,407,067 + Pf1N1B4_1236 0.35 -0.3
1,407,067 + Pf1N1B4_1236 0.35 -1.6
1,407,068 - Pf1N1B4_1236 0.35 +0.6
1,407,068 - Pf1N1B4_1236 0.35 -1.2
1,407,068 - Pf1N1B4_1236 0.35 -1.0
1,407,068 - Pf1N1B4_1236 0.35 +0.0
1,407,354 + Pf1N1B4_1236 0.44 -2.6
1,407,430 + +1.0
1,407,463 - Pf1N1B4_1236 0.47 -0.8
1,407,546 - Pf1N1B4_1236 0.49 +0.0
1,407,799 - Pf1N1B4_1236 0.57 +0.0
1,408,006 + Pf1N1B4_1236 0.62 -2.4
1,408,006 + Pf1N1B4_1236 0.62 +1.0
1,408,006 + +2.0
1,408,007 - Pf1N1B4_1236 0.63 -1.3
1,408,007 - Pf1N1B4_1236 0.63 -0.6
1,408,095 + +0.4
1,408,096 - +1.0
1,408,294 + Pf1N1B4_1236 0.71 +0.0
1,408,299 - Pf1N1B4_1236 0.71 -0.3
1,408,299 - Pf1N1B4_1236 0.71 +0.0
1,408,299 - +0.0
1,408,328 + Pf1N1B4_1236 0.72 +0.0
1,408,328 + Pf1N1B4_1236 0.72 +0.7
1,408,334 - Pf1N1B4_1236 0.72 -2.3
1,408,442 - Pf1N1B4_1236 0.75 +0.4
1,408,880 + Pf1N1B4_1236 0.88 -0.7
1,408,880 + Pf1N1B4_1236 0.88 -1.6
1,408,885 - Pf1N1B4_1236 0.88 -1.6
1,408,885 - Pf1N1B4_1236 0.88 +0.0
1,408,885 - Pf1N1B4_1236 0.88 -1.8
1,409,167 - -1.5
1,409,267 + -0.2
1,409,267 + -1.1
1,409,268 - -1.1
1,409,268 - -0.4
1,409,300 + -0.2
1,409,301 - +0.3
1,409,301 - -0.8
1,409,301 - -0.6
1,409,320 + -0.3
1,409,330 - +1.2
1,409,342 + -0.7

Or see this region's nucleotide sequence