Experiment: PYE with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_02278 and CCNA_02279 overlap by 4 nucleotides CCNA_02279 and CCNA_02280 overlap by 4 nucleotides CCNA_02280 and CCNA_02281 are separated by 91 nucleotides
CCNA_02278: CCNA_02278 - hypothetical protein, at 2,429,076 to 2,429,684
_02278
CCNA_02279: CCNA_02279 - disulfide bond formation protein B, at 2,429,681 to 2,430,310
_02279
CCNA_02280: CCNA_02280 - 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase, at 2,430,307 to 2,430,867
_02280
CCNA_02281: CCNA_02281 - membrane alanine aminopeptidase, at 2,430,959 to 2,432,740
_02281
Position (kb)
2430
2431 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 2429.309 kb on + strand, within CCNA_02278 at 2429.327 kb on + strand, within CCNA_02278 at 2429.345 kb on + strand, within CCNA_02278 at 2429.395 kb on + strand, within CCNA_02278 at 2429.411 kb on + strand, within CCNA_02278 at 2429.446 kb on - strand, within CCNA_02278 at 2429.491 kb on + strand, within CCNA_02278 at 2429.511 kb on + strand, within CCNA_02278 at 2429.538 kb on + strand, within CCNA_02278 at 2429.546 kb on - strand, within CCNA_02278 at 2429.564 kb on + strand, within CCNA_02278 at 2429.580 kb on + strand, within CCNA_02278 at 2429.594 kb on - strand, within CCNA_02278 at 2429.627 kb on - strand at 2429.661 kb on - strand at 2429.701 kb on - strand at 2429.718 kb on - strand at 2429.722 kb on - strand at 2429.740 kb on + strand at 2429.740 kb on + strand at 2429.740 kb on + strand at 2429.740 kb on + strand at 2429.740 kb on + strand at 2429.740 kb on + strand at 2429.740 kb on + strand at 2429.740 kb on + strand at 2429.747 kb on + strand, within CCNA_02279 at 2429.748 kb on - strand, within CCNA_02279 at 2429.797 kb on - strand, within CCNA_02279 at 2429.804 kb on + strand, within CCNA_02279 at 2429.824 kb on - strand, within CCNA_02279 at 2430.299 kb on - strand at 2430.869 kb on - strand at 2430.869 kb on - strand at 2430.935 kb on + strand at 2430.977 kb on - strand at 2431.041 kb on + strand at 2431.053 kb on + strand at 2431.063 kb on - strand at 2431.123 kb on + strand at 2431.187 kb on - strand, within CCNA_02281 at 2431.297 kb on + strand, within CCNA_02281 at 2431.297 kb on + strand, within CCNA_02281 at 2431.304 kb on + strand, within CCNA_02281 at 2431.310 kb on - strand, within CCNA_02281 at 2431.320 kb on + strand, within CCNA_02281 at 2431.320 kb on + strand, within CCNA_02281 at 2431.328 kb on - strand, within CCNA_02281 at 2431.388 kb on + strand, within CCNA_02281 at 2431.417 kb on - strand, within CCNA_02281 at 2431.430 kb on - strand, within CCNA_02281 at 2431.480 kb on - strand, within CCNA_02281 at 2431.495 kb on + strand, within CCNA_02281 at 2431.495 kb on + strand, within CCNA_02281 at 2431.503 kb on - strand, within CCNA_02281 at 2431.513 kb on - strand, within CCNA_02281 at 2431.546 kb on + strand, within CCNA_02281 at 2431.552 kb on + strand, within CCNA_02281 at 2431.554 kb on - strand, within CCNA_02281 at 2431.597 kb on + strand, within CCNA_02281 at 2431.597 kb on + strand, within CCNA_02281 at 2431.597 kb on - strand, within CCNA_02281 at 2431.634 kb on - strand, within CCNA_02281 at 2431.634 kb on - strand, within CCNA_02281 at 2431.648 kb on + strand, within CCNA_02281 at 2431.657 kb on - strand, within CCNA_02281 at 2431.657 kb on - strand, within CCNA_02281 at 2431.699 kb on - strand, within CCNA_02281 at 2431.771 kb on + strand, within CCNA_02281 at 2431.771 kb on + strand, within CCNA_02281 at 2431.772 kb on + strand, within CCNA_02281 at 2431.779 kb on - strand, within CCNA_02281 at 2431.830 kb on + strand, within CCNA_02281 at 2431.839 kb on - strand, within CCNA_02281 at 2431.850 kb on + strand, within CCNA_02281
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Polymyxin B sulfate 0.005 mg/ml remove 2,429,309 + CCNA_02278 0.38 +1.7 2,429,327 + CCNA_02278 0.41 +1.0 2,429,345 + CCNA_02278 0.44 +4.9 2,429,395 + CCNA_02278 0.52 +0.8 2,429,411 + CCNA_02278 0.55 +1.6 2,429,446 - CCNA_02278 0.61 +0.6 2,429,491 + CCNA_02278 0.68 +1.3 2,429,511 + CCNA_02278 0.71 +0.7 2,429,538 + CCNA_02278 0.76 +0.2 2,429,546 - CCNA_02278 0.77 -1.5 2,429,564 + CCNA_02278 0.80 +0.4 2,429,580 + CCNA_02278 0.83 +1.0 2,429,594 - CCNA_02278 0.85 +1.4 2,429,627 - -1.4 2,429,661 - +1.9 2,429,701 - +0.6 2,429,718 - -1.3 2,429,722 - -0.1 2,429,740 + +1.7 2,429,740 + +1.2 2,429,740 + +0.4 2,429,740 + +1.7 2,429,740 + +0.2 2,429,740 + +2.1 2,429,740 + +2.0 2,429,740 + +1.6 2,429,747 + CCNA_02279 0.10 +3.0 2,429,748 - CCNA_02279 0.11 +0.0 2,429,797 - CCNA_02279 0.18 -0.3 2,429,804 + CCNA_02279 0.20 +1.7 2,429,824 - CCNA_02279 0.23 +0.3 2,430,299 - +0.3 2,430,869 - -0.1 2,430,869 - +1.6 2,430,935 + -0.8 2,430,977 - +0.9 2,431,041 + -0.7 2,431,053 + -0.8 2,431,063 - -0.7 2,431,123 + -0.3 2,431,187 - CCNA_02281 0.13 +1.4 2,431,297 + CCNA_02281 0.19 +0.4 2,431,297 + CCNA_02281 0.19 -1.0 2,431,304 + CCNA_02281 0.19 -0.7 2,431,310 - CCNA_02281 0.20 +0.1 2,431,320 + CCNA_02281 0.20 -0.8 2,431,320 + CCNA_02281 0.20 -2.0 2,431,328 - CCNA_02281 0.21 +1.3 2,431,388 + CCNA_02281 0.24 +0.6 2,431,417 - CCNA_02281 0.26 -0.1 2,431,430 - CCNA_02281 0.26 -0.6 2,431,480 - CCNA_02281 0.29 +0.5 2,431,495 + CCNA_02281 0.30 -1.1 2,431,495 + CCNA_02281 0.30 +1.6 2,431,503 - CCNA_02281 0.31 -0.3 2,431,513 - CCNA_02281 0.31 -3.4 2,431,546 + CCNA_02281 0.33 -0.0 2,431,552 + CCNA_02281 0.33 -0.4 2,431,554 - CCNA_02281 0.33 -1.4 2,431,597 + CCNA_02281 0.36 +0.9 2,431,597 + CCNA_02281 0.36 +0.1 2,431,597 - CCNA_02281 0.36 +0.7 2,431,634 - CCNA_02281 0.38 -0.4 2,431,634 - CCNA_02281 0.38 -1.2 2,431,648 + CCNA_02281 0.39 -0.9 2,431,657 - CCNA_02281 0.39 -1.0 2,431,657 - CCNA_02281 0.39 -0.8 2,431,699 - CCNA_02281 0.42 +0.4 2,431,771 + CCNA_02281 0.46 +1.7 2,431,771 + CCNA_02281 0.46 -0.2 2,431,772 + CCNA_02281 0.46 -0.6 2,431,779 - CCNA_02281 0.46 +1.9 2,431,830 + CCNA_02281 0.49 -0.6 2,431,839 - CCNA_02281 0.49 -0.3 2,431,850 + CCNA_02281 0.50 +0.9
Or see this region's nucleotide sequence