Experiment: PYE with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01723 and CCNA_01724 are separated by 152 nucleotides CCNA_01724 and CCNA_01725 overlap by 4 nucleotides CCNA_01725 and CCNA_01726 are separated by 125 nucleotides
CCNA_01723: CCNA_01723 - 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 1,849,599 to 1,850,426
_01723
CCNA_01724: CCNA_01724 - hypothetical protein, at 1,850,579 to 1,851,331
_01724
CCNA_01725: CCNA_01725 - beta-barrel assembly machine (BAM) protein BamB, at 1,851,328 to 1,852,758
_01725
CCNA_01726: CCNA_01726 - EngA-subfamily GTPase protein, at 1,852,884 to 1,854,650
_01726
Position (kb)
1851
1852
1853 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1850.421 kb on + strand at 1850.454 kb on + strand at 1850.454 kb on + strand at 1850.456 kb on + strand at 1850.462 kb on - strand at 1850.503 kb on + strand at 1850.526 kb on + strand at 1850.569 kb on + strand at 1850.623 kb on + strand at 1850.653 kb on + strand at 1850.743 kb on + strand, within CCNA_01724 at 1850.812 kb on + strand, within CCNA_01724 at 1850.974 kb on + strand, within CCNA_01724 at 1851.244 kb on - strand, within CCNA_01724 at 1851.244 kb on - strand, within CCNA_01724 at 1851.250 kb on + strand, within CCNA_01724 at 1851.250 kb on + strand, within CCNA_01724 at 1851.250 kb on + strand, within CCNA_01724 at 1852.863 kb on + strand at 1852.888 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Polymyxin B sulfate 0.005 mg/ml remove 1,850,421 + +0.7 1,850,454 + +0.9 1,850,454 + +1.7 1,850,456 + +2.3 1,850,462 - +2.0 1,850,503 + +2.1 1,850,526 + +0.1 1,850,569 + +1.4 1,850,623 + -1.0 1,850,653 + -0.9 1,850,743 + CCNA_01724 0.22 +0.6 1,850,812 + CCNA_01724 0.31 -0.0 1,850,974 + CCNA_01724 0.52 +2.0 1,851,244 - CCNA_01724 0.88 +0.5 1,851,244 - CCNA_01724 0.88 -0.3 1,851,250 + CCNA_01724 0.89 +0.6 1,851,250 + CCNA_01724 0.89 +3.7 1,851,250 + CCNA_01724 0.89 +1.4 1,852,863 + +0.7 1,852,888 - +0.2
Or see this region's nucleotide sequence