Experiment: PYE with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01721 and CCNA_01722 overlap by 32 nucleotides CCNA_01722 and CCNA_01723 are separated by 92 nucleotides CCNA_01723 and CCNA_01724 are separated by 152 nucleotides CCNA_01724 and CCNA_01725 overlap by 4 nucleotides
CCNA_01721: CCNA_01721 - RNA polymerase ECF sigma factor, at 1,848,549 to 1,849,133
_01721
CCNA_01722: CCNA_01722 - hypothetical protein, at 1,849,102 to 1,849,506
_01722
CCNA_01723: CCNA_01723 - 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 1,849,599 to 1,850,426
_01723
CCNA_01724: CCNA_01724 - hypothetical protein, at 1,850,579 to 1,851,331
_01724
CCNA_01725: CCNA_01725 - beta-barrel assembly machine (BAM) protein BamB, at 1,851,328 to 1,852,758
_01725
Position (kb)
1849
1850
1851 Strain fitness (log2 ratio)
-1
0
1
2
3
4
5 at 1848.614 kb on - strand, within CCNA_01721 at 1848.629 kb on + strand, within CCNA_01721 at 1848.666 kb on + strand, within CCNA_01721 at 1848.674 kb on - strand, within CCNA_01721 at 1848.675 kb on - strand, within CCNA_01721 at 1848.731 kb on + strand, within CCNA_01721 at 1848.812 kb on + strand, within CCNA_01721 at 1848.812 kb on + strand, within CCNA_01721 at 1848.820 kb on - strand, within CCNA_01721 at 1848.820 kb on - strand, within CCNA_01721 at 1848.827 kb on + strand, within CCNA_01721 at 1848.881 kb on - strand, within CCNA_01721 at 1848.936 kb on + strand, within CCNA_01721 at 1848.936 kb on + strand, within CCNA_01721 at 1848.988 kb on + strand, within CCNA_01721 at 1849.044 kb on + strand, within CCNA_01721 at 1849.052 kb on - strand, within CCNA_01721 at 1849.122 kb on + strand at 1849.122 kb on + strand at 1849.122 kb on + strand at 1849.122 kb on + strand at 1849.130 kb on - strand at 1849.198 kb on + strand at 1849.200 kb on + strand, within CCNA_01722 at 1849.277 kb on + strand, within CCNA_01722 at 1849.282 kb on + strand, within CCNA_01722 at 1849.282 kb on + strand, within CCNA_01722 at 1849.520 kb on + strand at 1850.421 kb on + strand at 1850.454 kb on + strand at 1850.454 kb on + strand at 1850.456 kb on + strand at 1850.462 kb on - strand at 1850.503 kb on + strand at 1850.526 kb on + strand at 1850.569 kb on + strand at 1850.623 kb on + strand at 1850.653 kb on + strand at 1850.743 kb on + strand, within CCNA_01724 at 1850.812 kb on + strand, within CCNA_01724 at 1850.974 kb on + strand, within CCNA_01724 at 1851.244 kb on - strand, within CCNA_01724 at 1851.244 kb on - strand, within CCNA_01724 at 1851.250 kb on + strand, within CCNA_01724 at 1851.250 kb on + strand, within CCNA_01724 at 1851.250 kb on + strand, within CCNA_01724
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Polymyxin B sulfate 0.005 mg/ml remove 1,848,614 - CCNA_01721 0.11 +2.3 1,848,629 + CCNA_01721 0.14 +1.8 1,848,666 + CCNA_01721 0.20 +0.6 1,848,674 - CCNA_01721 0.21 -0.5 1,848,675 - CCNA_01721 0.22 +1.2 1,848,731 + CCNA_01721 0.31 -0.1 1,848,812 + CCNA_01721 0.45 +0.4 1,848,812 + CCNA_01721 0.45 +2.8 1,848,820 - CCNA_01721 0.46 +0.7 1,848,820 - CCNA_01721 0.46 -0.4 1,848,827 + CCNA_01721 0.48 +0.4 1,848,881 - CCNA_01721 0.57 +0.9 1,848,936 + CCNA_01721 0.66 -0.4 1,848,936 + CCNA_01721 0.66 +2.5 1,848,988 + CCNA_01721 0.75 +0.8 1,849,044 + CCNA_01721 0.85 -0.4 1,849,052 - CCNA_01721 0.86 -1.1 1,849,122 + +2.0 1,849,122 + +0.1 1,849,122 + -1.3 1,849,122 + +0.9 1,849,130 - -0.7 1,849,198 + +3.9 1,849,200 + CCNA_01722 0.24 +1.7 1,849,277 + CCNA_01722 0.43 +3.7 1,849,282 + CCNA_01722 0.44 +5.4 1,849,282 + CCNA_01722 0.44 +5.1 1,849,520 + +0.3 1,850,421 + +0.7 1,850,454 + +0.9 1,850,454 + +1.7 1,850,456 + +2.3 1,850,462 - +2.0 1,850,503 + +2.1 1,850,526 + +0.1 1,850,569 + +1.4 1,850,623 + -1.0 1,850,653 + -0.9 1,850,743 + CCNA_01724 0.22 +0.6 1,850,812 + CCNA_01724 0.31 -0.0 1,850,974 + CCNA_01724 0.52 +2.0 1,851,244 - CCNA_01724 0.88 +0.5 1,851,244 - CCNA_01724 0.88 -0.3 1,851,250 + CCNA_01724 0.89 +0.6 1,851,250 + CCNA_01724 0.89 +3.7 1,851,250 + CCNA_01724 0.89 +1.4
Or see this region's nucleotide sequence