Experiment: PYE with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01302 and CCNA_01303 are separated by 112 nucleotides CCNA_01303 and CCNA_01304 are separated by 228 nucleotides CCNA_01304 and CCNA_01305 are separated by 13 nucleotides CCNA_01305 and CCNA_01306 overlap by 23 nucleotides
CCNA_01302: CCNA_01302 - acetyltransferase domain protein, at 1,428,800 to 1,429,948
_01302
CCNA_01303: CCNA_01303 - hypothetical protein, at 1,430,061 to 1,430,900
_01303
CCNA_01304: CCNA_01304 - hypothetical protein, at 1,431,129 to 1,431,329
_01304
CCNA_01305: CCNA_01305 - SSU ribosomal protein S10P, at 1,431,343 to 1,431,651
_01305
CCNA_01306: CCNA_01306 - LSU ribosomal protein L3P, at 1,431,629 to 1,432,432
_01306
Position (kb)
1430
1431 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1429.120 kb on - strand, within CCNA_01302 at 1429.158 kb on - strand, within CCNA_01302 at 1429.264 kb on + strand, within CCNA_01302 at 1429.287 kb on + strand, within CCNA_01302 at 1429.299 kb on + strand, within CCNA_01302 at 1429.299 kb on + strand, within CCNA_01302 at 1429.307 kb on + strand, within CCNA_01302 at 1429.315 kb on - strand, within CCNA_01302 at 1429.315 kb on - strand, within CCNA_01302 at 1429.391 kb on + strand, within CCNA_01302 at 1429.440 kb on - strand, within CCNA_01302 at 1429.447 kb on - strand, within CCNA_01302 at 1429.458 kb on - strand, within CCNA_01302 at 1429.481 kb on + strand, within CCNA_01302 at 1429.493 kb on + strand, within CCNA_01302 at 1429.497 kb on + strand, within CCNA_01302 at 1429.498 kb on + strand, within CCNA_01302 at 1429.518 kb on + strand, within CCNA_01302 at 1429.542 kb on + strand, within CCNA_01302 at 1429.612 kb on + strand, within CCNA_01302 at 1429.617 kb on + strand, within CCNA_01302 at 1429.620 kb on - strand, within CCNA_01302 at 1429.626 kb on - strand, within CCNA_01302 at 1429.635 kb on + strand, within CCNA_01302 at 1429.640 kb on + strand, within CCNA_01302 at 1429.648 kb on - strand, within CCNA_01302 at 1429.648 kb on - strand, within CCNA_01302 at 1429.648 kb on - strand, within CCNA_01302 at 1429.678 kb on + strand, within CCNA_01302 at 1429.686 kb on - strand, within CCNA_01302 at 1429.720 kb on - strand, within CCNA_01302 at 1429.745 kb on + strand, within CCNA_01302 at 1429.818 kb on + strand, within CCNA_01302 at 1429.826 kb on - strand, within CCNA_01302 at 1429.826 kb on - strand, within CCNA_01302 at 1429.838 kb on + strand at 1429.845 kb on - strand at 1429.847 kb on + strand at 1429.889 kb on - strand at 1429.901 kb on - strand at 1429.912 kb on - strand at 1429.938 kb on + strand at 1429.947 kb on + strand at 1429.950 kb on - strand at 1429.950 kb on - strand at 1429.952 kb on + strand at 1429.955 kb on - strand at 1429.956 kb on - strand at 1430.006 kb on + strand at 1430.052 kb on - strand at 1430.085 kb on + strand at 1430.085 kb on + strand at 1430.093 kb on - strand at 1430.093 kb on - strand at 1430.093 kb on - strand at 1430.183 kb on + strand, within CCNA_01303 at 1430.183 kb on + strand, within CCNA_01303 at 1430.192 kb on + strand, within CCNA_01303 at 1430.193 kb on - strand, within CCNA_01303 at 1430.223 kb on + strand, within CCNA_01303 at 1430.276 kb on + strand, within CCNA_01303 at 1430.283 kb on - strand, within CCNA_01303 at 1430.316 kb on + strand, within CCNA_01303 at 1430.324 kb on - strand, within CCNA_01303 at 1430.329 kb on - strand, within CCNA_01303 at 1430.344 kb on - strand, within CCNA_01303 at 1430.349 kb on - strand, within CCNA_01303 at 1430.452 kb on + strand, within CCNA_01303 at 1430.457 kb on + strand, within CCNA_01303 at 1430.500 kb on - strand, within CCNA_01303 at 1430.506 kb on - strand, within CCNA_01303 at 1430.506 kb on - strand, within CCNA_01303 at 1430.529 kb on + strand, within CCNA_01303 at 1430.595 kb on + strand, within CCNA_01303 at 1430.635 kb on - strand, within CCNA_01303 at 1430.654 kb on - strand, within CCNA_01303 at 1430.708 kb on + strand, within CCNA_01303 at 1430.716 kb on - strand, within CCNA_01303 at 1430.716 kb on - strand, within CCNA_01303 at 1430.716 kb on - strand, within CCNA_01303 at 1430.747 kb on - strand, within CCNA_01303 at 1430.789 kb on - strand, within CCNA_01303 at 1430.825 kb on + strand at 1430.864 kb on + strand at 1430.885 kb on - strand at 1430.890 kb on + strand at 1430.900 kb on + strand at 1430.931 kb on + strand at 1430.939 kb on - strand at 1430.953 kb on + strand at 1430.983 kb on - strand at 1431.021 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Polymyxin B sulfate 0.005 mg/ml remove 1,429,120 - CCNA_01302 0.28 -0.6 1,429,158 - CCNA_01302 0.31 -1.5 1,429,264 + CCNA_01302 0.40 -0.1 1,429,287 + CCNA_01302 0.42 -2.5 1,429,299 + CCNA_01302 0.43 +1.0 1,429,299 + CCNA_01302 0.43 -0.2 1,429,307 + CCNA_01302 0.44 -0.5 1,429,315 - CCNA_01302 0.45 +1.1 1,429,315 - CCNA_01302 0.45 -0.5 1,429,391 + CCNA_01302 0.51 -1.1 1,429,440 - CCNA_01302 0.56 -0.4 1,429,447 - CCNA_01302 0.56 -0.2 1,429,458 - CCNA_01302 0.57 +0.3 1,429,481 + CCNA_01302 0.59 -0.4 1,429,493 + CCNA_01302 0.60 +0.1 1,429,497 + CCNA_01302 0.61 -1.0 1,429,498 + CCNA_01302 0.61 +0.0 1,429,518 + CCNA_01302 0.62 -1.3 1,429,542 + CCNA_01302 0.65 +1.3 1,429,612 + CCNA_01302 0.71 -1.5 1,429,617 + CCNA_01302 0.71 -1.2 1,429,620 - CCNA_01302 0.71 +1.5 1,429,626 - CCNA_01302 0.72 +1.0 1,429,635 + CCNA_01302 0.73 -0.2 1,429,640 + CCNA_01302 0.73 +0.1 1,429,648 - CCNA_01302 0.74 -0.9 1,429,648 - CCNA_01302 0.74 +0.6 1,429,648 - CCNA_01302 0.74 -1.3 1,429,678 + CCNA_01302 0.76 -0.2 1,429,686 - CCNA_01302 0.77 +0.1 1,429,720 - CCNA_01302 0.80 -1.3 1,429,745 + CCNA_01302 0.82 +0.3 1,429,818 + CCNA_01302 0.89 -1.7 1,429,826 - CCNA_01302 0.89 -1.6 1,429,826 - CCNA_01302 0.89 -0.1 1,429,838 + -0.1 1,429,845 - +2.5 1,429,847 + -0.5 1,429,889 - -1.8 1,429,901 - -1.5 1,429,912 - -0.9 1,429,938 + +2.0 1,429,947 + +0.7 1,429,950 - +2.0 1,429,950 - +0.2 1,429,952 + +0.0 1,429,955 - +0.4 1,429,956 - -0.3 1,430,006 + -1.5 1,430,052 - -0.2 1,430,085 + +2.1 1,430,085 + +3.0 1,430,093 - +0.6 1,430,093 - -1.0 1,430,093 - -0.3 1,430,183 + CCNA_01303 0.15 +0.7 1,430,183 + CCNA_01303 0.15 +0.1 1,430,192 + CCNA_01303 0.16 +0.7 1,430,193 - CCNA_01303 0.16 +0.4 1,430,223 + CCNA_01303 0.19 -1.3 1,430,276 + CCNA_01303 0.26 +3.7 1,430,283 - CCNA_01303 0.26 -2.8 1,430,316 + CCNA_01303 0.30 +1.0 1,430,324 - CCNA_01303 0.31 +0.3 1,430,329 - CCNA_01303 0.32 +2.0 1,430,344 - CCNA_01303 0.34 +0.2 1,430,349 - CCNA_01303 0.34 -0.3 1,430,452 + CCNA_01303 0.47 +0.5 1,430,457 + CCNA_01303 0.47 +0.3 1,430,500 - CCNA_01303 0.52 -1.3 1,430,506 - CCNA_01303 0.53 +0.3 1,430,506 - CCNA_01303 0.53 +1.0 1,430,529 + CCNA_01303 0.56 +1.1 1,430,595 + CCNA_01303 0.64 +0.2 1,430,635 - CCNA_01303 0.68 -1.9 1,430,654 - CCNA_01303 0.71 +1.2 1,430,708 + CCNA_01303 0.77 +2.1 1,430,716 - CCNA_01303 0.78 +1.3 1,430,716 - CCNA_01303 0.78 -2.6 1,430,716 - CCNA_01303 0.78 -1.7 1,430,747 - CCNA_01303 0.82 -0.1 1,430,789 - CCNA_01303 0.87 -0.5 1,430,825 + +2.3 1,430,864 + +1.7 1,430,885 - -2.0 1,430,890 + +0.7 1,430,900 + +1.3 1,430,931 + +1.2 1,430,939 - +0.3 1,430,953 + +1.0 1,430,983 - -0.6 1,431,021 + +0.2
Or see this region's nucleotide sequence