Strain Fitness in Caulobacter crescentus NA1000 around CCNA_00898

Experiment: PYE with Polymyxin B sulfate 0.005 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntCCNA_00895 and CCNA_00896 are separated by 7 nucleotidesCCNA_00896 and CCNA_00897 are separated by 92 nucleotidesCCNA_00897 and CCNA_00898 are separated by 75 nucleotidesCCNA_00898 and CCNA_00899 are separated by 3 nucleotides CCNA_00895: CCNA_00895 - hypothetical protein, at 975,146 to 975,541 _00895 CCNA_00896: CCNA_00896 - hypothetical protein, at 975,549 to 975,920 _00896 CCNA_00897: CCNA_00897 - hypothetical protein, at 976,013 to 976,177 _00897 CCNA_00898: CCNA_00898 - ribosomal large subunit pseudouridine synthase F, at 976,253 to 977,020 _00898 CCNA_00899: CCNA_00899 - bacterial peptide chain Release factor 1 (RF-1), at 977,024 to 978,094 _00899 Position (kb) 976 977 978Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 975.271 kb on + strand, within CCNA_00895at 975.326 kb on + strand, within CCNA_00895at 975.453 kb on + strand, within CCNA_00895at 975.453 kb on + strand, within CCNA_00895at 975.501 kb on + strand, within CCNA_00895at 975.501 kb on + strand, within CCNA_00895at 975.535 kb on + strandat 975.540 kb on + strandat 975.553 kb on + strandat 975.558 kb on + strandat 975.566 kb on - strandat 975.653 kb on + strand, within CCNA_00896at 975.672 kb on - strand, within CCNA_00896at 975.710 kb on + strand, within CCNA_00896at 975.733 kb on - strand, within CCNA_00896at 975.805 kb on - strand, within CCNA_00896at 975.827 kb on - strand, within CCNA_00896at 975.828 kb on - strand, within CCNA_00896at 975.888 kb on + strandat 975.888 kb on + strandat 975.902 kb on + strandat 976.051 kb on - strand, within CCNA_00897at 976.098 kb on + strand, within CCNA_00897at 976.106 kb on - strand, within CCNA_00897at 976.145 kb on - strand, within CCNA_00897at 976.152 kb on - strand, within CCNA_00897at 976.168 kb on + strandat 976.181 kb on - strandat 976.191 kb on - strandat 976.195 kb on - strandat 976.234 kb on + strandat 976.234 kb on + strandat 976.240 kb on + strandat 976.242 kb on - strandat 976.242 kb on - strandat 976.275 kb on - strandat 976.324 kb on + strandat 976.324 kb on + strandat 976.324 kb on + strandat 976.332 kb on - strand, within CCNA_00898at 976.332 kb on - strand, within CCNA_00898at 976.332 kb on - strand, within CCNA_00898at 976.332 kb on - strand, within CCNA_00898at 976.332 kb on - strand, within CCNA_00898at 976.333 kb on - strand, within CCNA_00898at 976.357 kb on - strand, within CCNA_00898at 976.362 kb on + strand, within CCNA_00898at 976.362 kb on + strand, within CCNA_00898at 976.404 kb on - strand, within CCNA_00898at 976.415 kb on + strand, within CCNA_00898at 976.420 kb on + strand, within CCNA_00898at 976.420 kb on + strand, within CCNA_00898at 976.420 kb on + strand, within CCNA_00898at 976.428 kb on - strand, within CCNA_00898at 976.428 kb on - strand, within CCNA_00898at 976.428 kb on - strand, within CCNA_00898at 976.446 kb on - strand, within CCNA_00898at 976.449 kb on + strand, within CCNA_00898at 976.451 kb on - strand, within CCNA_00898at 976.479 kb on - strand, within CCNA_00898at 976.481 kb on + strand, within CCNA_00898at 976.500 kb on - strand, within CCNA_00898at 976.712 kb on - strand, within CCNA_00898at 976.745 kb on - strand, within CCNA_00898at 976.760 kb on - strand, within CCNA_00898at 976.799 kb on + strand, within CCNA_00898at 976.807 kb on - strand, within CCNA_00898at 976.807 kb on - strand, within CCNA_00898at 976.807 kb on - strand, within CCNA_00898at 976.809 kb on + strand, within CCNA_00898at 976.817 kb on - strand, within CCNA_00898at 976.817 kb on - strand, within CCNA_00898at 976.824 kb on - strand, within CCNA_00898at 976.840 kb on + strand, within CCNA_00898at 976.840 kb on + strand, within CCNA_00898at 976.840 kb on + strand, within CCNA_00898at 976.847 kb on + strand, within CCNA_00898at 976.847 kb on + strand, within CCNA_00898at 976.855 kb on - strand, within CCNA_00898at 976.888 kb on - strand, within CCNA_00898at 976.950 kb on + strandat 976.955 kb on + strandat 976.955 kb on + strandat 976.963 kb on - strandat 977.004 kb on - strandat 977.004 kb on - strandat 977.004 kb on - strandat 977.012 kb on - strandat 977.026 kb on + strandat 977.038 kb on + strandat 977.038 kb on + strandat 977.043 kb on + strandat 977.046 kb on - strandat 977.046 kb on - strandat 977.046 kb on - strandat 977.056 kb on - strandat 977.069 kb on - strandat 977.069 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction PYE with Polymyxin B sulfate 0.005 mg/ml
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975,271 + CCNA_00895 0.32 +2.2
975,326 + CCNA_00895 0.45 +1.1
975,453 + CCNA_00895 0.78 +2.6
975,453 + CCNA_00895 0.78 +2.6
975,501 + CCNA_00895 0.90 +2.1
975,501 + CCNA_00895 0.90 +2.4
975,535 + +2.6
975,540 + +1.7
975,553 + +1.8
975,558 + +3.0
975,566 - -2.0
975,653 + CCNA_00896 0.28 +1.3
975,672 - CCNA_00896 0.33 -1.3
975,710 + CCNA_00896 0.43 +0.9
975,733 - CCNA_00896 0.49 +1.7
975,805 - CCNA_00896 0.69 +0.1
975,827 - CCNA_00896 0.75 -0.5
975,828 - CCNA_00896 0.75 -0.8
975,888 + +1.8
975,888 + +1.9
975,902 + +1.8
976,051 - CCNA_00897 0.23 -0.2
976,098 + CCNA_00897 0.52 +0.0
976,106 - CCNA_00897 0.56 -0.2
976,145 - CCNA_00897 0.80 +1.2
976,152 - CCNA_00897 0.84 +0.2
976,168 + -0.3
976,181 - +0.9
976,191 - +2.3
976,195 - +1.4
976,234 + -1.5
976,234 + -0.8
976,240 + +0.5
976,242 - +0.7
976,242 - +1.6
976,275 - +1.6
976,324 + -0.7
976,324 + +0.3
976,324 + -0.6
976,332 - CCNA_00898 0.10 +0.5
976,332 - CCNA_00898 0.10 +3.3
976,332 - CCNA_00898 0.10 +0.0
976,332 - CCNA_00898 0.10 +0.8
976,332 - CCNA_00898 0.10 +4.0
976,333 - CCNA_00898 0.10 +3.0
976,357 - CCNA_00898 0.14 +1.0
976,362 + CCNA_00898 0.14 +0.3
976,362 + CCNA_00898 0.14 -0.3
976,404 - CCNA_00898 0.20 +0.7
976,415 + CCNA_00898 0.21 -0.7
976,420 + CCNA_00898 0.22 -0.7
976,420 + CCNA_00898 0.22 +0.6
976,420 + CCNA_00898 0.22 -0.5
976,428 - CCNA_00898 0.23 +1.6
976,428 - CCNA_00898 0.23 +2.2
976,428 - CCNA_00898 0.23 +0.7
976,446 - CCNA_00898 0.25 +2.5
976,449 + CCNA_00898 0.26 +0.7
976,451 - CCNA_00898 0.26 +0.7
976,479 - CCNA_00898 0.29 +1.3
976,481 + CCNA_00898 0.30 +1.0
976,500 - CCNA_00898 0.32 +1.0
976,712 - CCNA_00898 0.60 +1.0
976,745 - CCNA_00898 0.64 +1.8
976,760 - CCNA_00898 0.66 +0.8
976,799 + CCNA_00898 0.71 -1.4
976,807 - CCNA_00898 0.72 +0.8
976,807 - CCNA_00898 0.72 +1.3
976,807 - CCNA_00898 0.72 +0.3
976,809 + CCNA_00898 0.72 +1.9
976,817 - CCNA_00898 0.73 +0.1
976,817 - CCNA_00898 0.73 +1.6
976,824 - CCNA_00898 0.74 +0.1
976,840 + CCNA_00898 0.76 -1.5
976,840 + CCNA_00898 0.76 +1.2
976,840 + CCNA_00898 0.76 -0.3
976,847 + CCNA_00898 0.77 -0.5
976,847 + CCNA_00898 0.77 -1.7
976,855 - CCNA_00898 0.78 +1.1
976,888 - CCNA_00898 0.83 +0.7
976,950 + -1.4
976,955 + -1.5
976,955 + -0.3
976,963 - -2.0
977,004 - +0.4
977,004 - +1.6
977,004 - +0.9
977,012 - +1.3
977,026 + +0.0
977,038 + +1.7
977,038 + -1.6
977,043 + +0.8
977,046 - +1.2
977,046 - +1.3
977,046 - -0.4
977,056 - +0.4
977,069 - +1.4
977,069 - +0.9

Or see this region's nucleotide sequence