Experiment: PYE with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00420 and CCNA_00421 overlap by 4 nucleotides CCNA_00421 and CCNA_00422 are separated by 49 nucleotides CCNA_00422 and CCNA_03919 are separated by 1 nucleotides CCNA_03919 and CCNA_00423 are separated by 233 nucleotides
CCNA_00420: CCNA_00420 - hypothetical protein, at 431,334 to 431,885
_00420
CCNA_00421: CCNA_00421 - dihydroneopterin aldolase, at 431,882 to 432,286
_00421
CCNA_00422: CCNA_00422 - SDR pteridine reductase, at 432,336 to 433,088
_00422
CCNA_03919: CCNA_03919 - hypothetical protein, at 433,090 to 433,170
_03919
CCNA_00423: CCNA_00423 - phosphohydrolase, MutT-nudix family, at 433,404 to 433,874
_00423
Position (kb) 432
433
434 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 431.366 kb on + strand at 431.366 kb on + strand at 431.374 kb on - strand at 431.402 kb on - strand, within CCNA_00420 at 431.402 kb on - strand, within CCNA_00420 at 431.402 kb on - strand, within CCNA_00420 at 431.435 kb on - strand, within CCNA_00420 at 431.444 kb on + strand, within CCNA_00420 at 431.444 kb on + strand, within CCNA_00420 at 431.444 kb on + strand, within CCNA_00420 at 431.444 kb on + strand, within CCNA_00420 at 431.444 kb on + strand, within CCNA_00420 at 431.444 kb on + strand, within CCNA_00420 at 431.444 kb on + strand, within CCNA_00420 at 431.452 kb on - strand, within CCNA_00420 at 431.473 kb on - strand, within CCNA_00420 at 431.473 kb on - strand, within CCNA_00420 at 431.490 kb on + strand, within CCNA_00420 at 431.490 kb on + strand, within CCNA_00420 at 431.498 kb on - strand, within CCNA_00420 at 431.498 kb on - strand, within CCNA_00420 at 431.529 kb on + strand, within CCNA_00420 at 431.529 kb on + strand, within CCNA_00420 at 431.529 kb on + strand, within CCNA_00420 at 431.529 kb on + strand, within CCNA_00420 at 431.529 kb on + strand, within CCNA_00420 at 431.531 kb on - strand, within CCNA_00420 at 431.534 kb on + strand, within CCNA_00420 at 431.537 kb on - strand, within CCNA_00420 at 431.537 kb on - strand, within CCNA_00420 at 431.537 kb on - strand, within CCNA_00420 at 431.537 kb on - strand, within CCNA_00420 at 431.539 kb on + strand, within CCNA_00420 at 431.539 kb on + strand, within CCNA_00420 at 431.545 kb on + strand, within CCNA_00420 at 431.556 kb on + strand, within CCNA_00420 at 431.568 kb on - strand, within CCNA_00420 at 431.575 kb on + strand, within CCNA_00420 at 431.593 kb on + strand, within CCNA_00420 at 431.593 kb on - strand, within CCNA_00420 at 431.594 kb on - strand, within CCNA_00420 at 431.598 kb on - strand, within CCNA_00420 at 431.613 kb on + strand, within CCNA_00420 at 431.621 kb on - strand, within CCNA_00420 at 431.621 kb on - strand, within CCNA_00420 at 431.662 kb on + strand, within CCNA_00420 at 431.670 kb on - strand, within CCNA_00420 at 431.670 kb on - strand, within CCNA_00420 at 431.679 kb on + strand, within CCNA_00420 at 431.757 kb on + strand, within CCNA_00420 at 431.842 kb on + strand at 431.842 kb on + strand at 432.309 kb on + strand at 432.314 kb on + strand at 432.322 kb on + strand at 432.330 kb on - strand at 432.333 kb on + strand at 432.341 kb on - strand at 432.344 kb on - strand at 432.374 kb on - strand at 432.396 kb on + strand at 432.404 kb on - strand at 432.435 kb on - strand, within CCNA_00422 at 432.452 kb on + strand, within CCNA_00422 at 432.460 kb on - strand, within CCNA_00422 at 432.533 kb on + strand, within CCNA_00422 at 432.536 kb on - strand, within CCNA_00422 at 432.545 kb on + strand, within CCNA_00422 at 432.587 kb on - strand, within CCNA_00422 at 432.588 kb on - strand at 432.591 kb on + strand, within CCNA_00422 at 432.591 kb on + strand, within CCNA_00422 at 432.598 kb on - strand, within CCNA_00422 at 432.616 kb on + strand, within CCNA_00422 at 432.621 kb on + strand, within CCNA_00422 at 432.626 kb on + strand, within CCNA_00422 at 432.724 kb on + strand, within CCNA_00422 at 432.732 kb on - strand, within CCNA_00422 at 432.760 kb on - strand, within CCNA_00422 at 432.793 kb on + strand, within CCNA_00422 at 432.824 kb on - strand, within CCNA_00422 at 432.903 kb on - strand, within CCNA_00422 at 432.920 kb on - strand, within CCNA_00422 at 432.944 kb on - strand, within CCNA_00422 at 432.963 kb on + strand, within CCNA_00422 at 432.984 kb on + strand, within CCNA_00422 at 433.007 kb on - strand, within CCNA_00422 at 433.039 kb on - strand at 433.062 kb on + strand at 433.067 kb on + strand at 433.070 kb on - strand at 433.070 kb on - strand at 433.070 kb on - strand at 433.070 kb on - strand at 433.075 kb on - strand at 433.102 kb on + strand, within CCNA_03919 at 433.116 kb on + strand, within CCNA_03919 at 433.124 kb on - strand, within CCNA_03919 at 433.125 kb on - strand, within CCNA_03919 at 433.136 kb on + strand, within CCNA_03919 at 433.136 kb on + strand, within CCNA_03919 at 433.144 kb on - strand, within CCNA_03919 at 433.144 kb on - strand, within CCNA_03919 at 433.148 kb on - strand, within CCNA_03919 at 433.172 kb on + strand at 433.205 kb on - strand at 433.233 kb on - strand at 433.245 kb on + strand at 433.253 kb on - strand at 433.264 kb on + strand at 433.268 kb on - strand at 433.268 kb on - strand at 433.304 kb on + strand at 433.306 kb on + strand at 433.306 kb on + strand at 433.306 kb on + strand at 433.306 kb on + strand at 433.306 kb on + strand at 433.319 kb on + strand at 433.338 kb on + strand at 433.338 kb on + strand at 433.338 kb on + strand at 433.338 kb on + strand at 433.346 kb on - strand at 433.346 kb on - strand at 433.378 kb on + strand at 433.390 kb on + strand at 433.390 kb on + strand at 433.397 kb on - strand at 433.406 kb on + strand at 433.406 kb on + strand at 433.414 kb on - strand at 433.445 kb on + strand at 433.445 kb on + strand at 433.452 kb on + strand, within CCNA_00423 at 433.453 kb on - strand, within CCNA_00423 at 433.453 kb on - strand, within CCNA_00423 at 433.474 kb on + strand, within CCNA_00423 at 433.474 kb on + strand, within CCNA_00423 at 433.474 kb on + strand, within CCNA_00423 at 433.474 kb on + strand, within CCNA_00423 at 433.474 kb on + strand, within CCNA_00423 at 433.474 kb on + strand, within CCNA_00423 at 433.474 kb on + strand, within CCNA_00423 at 433.482 kb on - strand, within CCNA_00423 at 433.514 kb on + strand, within CCNA_00423 at 433.514 kb on + strand, within CCNA_00423 at 433.522 kb on - strand, within CCNA_00423 at 433.538 kb on + strand, within CCNA_00423 at 433.541 kb on - strand, within CCNA_00423 at 433.546 kb on - strand, within CCNA_00423 at 433.552 kb on - strand, within CCNA_00423 at 433.559 kb on - strand, within CCNA_00423 at 433.560 kb on - strand, within CCNA_00423 at 433.668 kb on + strand, within CCNA_00423 at 433.683 kb on + strand, within CCNA_00423 at 433.696 kb on - strand, within CCNA_00423 at 433.707 kb on + strand, within CCNA_00423 at 433.707 kb on + strand, within CCNA_00423 at 433.741 kb on + strand, within CCNA_00423 at 433.749 kb on - strand, within CCNA_00423 at 433.815 kb on - strand, within CCNA_00423 at 433.839 kb on + strand at 433.844 kb on + strand at 433.846 kb on + strand at 433.851 kb on + strand at 433.851 kb on + strand at 433.855 kb on + strand at 433.859 kb on - strand at 433.863 kb on - strand at 433.870 kb on + strand at 433.873 kb on - strand at 433.875 kb on + strand at 433.878 kb on + strand at 433.899 kb on - strand at 433.915 kb on - strand at 433.925 kb on - strand at 433.926 kb on + strand at 433.949 kb on + strand at 433.949 kb on + strand at 433.949 kb on + strand at 433.949 kb on + strand at 433.949 kb on + strand at 433.949 kb on + strand at 433.957 kb on - strand at 433.957 kb on - strand at 433.957 kb on - strand at 433.957 kb on - strand at 433.957 kb on - strand at 433.957 kb on - strand at 433.965 kb on - strand at 433.965 kb on - strand at 433.965 kb on - strand at 433.996 kb on + strand at 433.997 kb on - strand at 434.033 kb on + strand at 434.041 kb on - strand at 434.041 kb on - strand at 434.041 kb on - strand at 434.060 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Polymyxin B sulfate 0.005 mg/ml remove 431,366 + -0.8 431,366 + -0.7 431,374 - -0.9 431,402 - CCNA_00420 0.12 -0.6 431,402 - CCNA_00420 0.12 +0.4 431,402 - CCNA_00420 0.12 +1.6 431,435 - CCNA_00420 0.18 +0.5 431,444 + CCNA_00420 0.20 -0.0 431,444 + CCNA_00420 0.20 -1.0 431,444 + CCNA_00420 0.20 +1.0 431,444 + CCNA_00420 0.20 -1.6 431,444 + CCNA_00420 0.20 -1.8 431,444 + CCNA_00420 0.20 -0.6 431,444 + CCNA_00420 0.20 -0.7 431,452 - CCNA_00420 0.21 -1.4 431,473 - CCNA_00420 0.25 -1.6 431,473 - CCNA_00420 0.25 +1.6 431,490 + CCNA_00420 0.28 -0.9 431,490 + CCNA_00420 0.28 -1.5 431,498 - CCNA_00420 0.30 +0.7 431,498 - CCNA_00420 0.30 -1.9 431,529 + CCNA_00420 0.35 -1.8 431,529 + CCNA_00420 0.35 -0.1 431,529 + CCNA_00420 0.35 -0.6 431,529 + CCNA_00420 0.35 -1.7 431,529 + CCNA_00420 0.35 -0.4 431,531 - CCNA_00420 0.36 +0.4 431,534 + CCNA_00420 0.36 -1.4 431,537 - CCNA_00420 0.37 +1.0 431,537 - CCNA_00420 0.37 +2.1 431,537 - CCNA_00420 0.37 +1.6 431,537 - CCNA_00420 0.37 +1.1 431,539 + CCNA_00420 0.37 +0.2 431,539 + CCNA_00420 0.37 +0.3 431,545 + CCNA_00420 0.38 -0.7 431,556 + CCNA_00420 0.40 -0.7 431,568 - CCNA_00420 0.42 +0.8 431,575 + CCNA_00420 0.44 -0.8 431,593 + CCNA_00420 0.47 -1.7 431,593 - CCNA_00420 0.47 +1.8 431,594 - CCNA_00420 0.47 +0.0 431,598 - CCNA_00420 0.48 -0.3 431,613 + CCNA_00420 0.51 -1.6 431,621 - CCNA_00420 0.52 +1.9 431,621 - CCNA_00420 0.52 -0.1 431,662 + CCNA_00420 0.59 +0.2 431,670 - CCNA_00420 0.61 +0.5 431,670 - CCNA_00420 0.61 +1.7 431,679 + CCNA_00420 0.62 -0.4 431,757 + CCNA_00420 0.77 -2.4 431,842 + -0.2 431,842 + -1.4 432,309 + -0.3 432,314 + +0.8 432,322 + +1.0 432,330 - +1.8 432,333 + +1.6 432,341 - +1.7 432,344 - +0.9 432,374 - -1.1 432,396 + +0.1 432,404 - -1.8 432,435 - CCNA_00422 0.13 -0.4 432,452 + CCNA_00422 0.15 -0.3 432,460 - CCNA_00422 0.16 -0.6 432,533 + CCNA_00422 0.26 +0.3 432,536 - CCNA_00422 0.27 -1.4 432,545 + CCNA_00422 0.28 +0.6 432,587 - CCNA_00422 0.33 -1.8 432,588 - +2.2 432,591 + CCNA_00422 0.34 +0.2 432,591 + CCNA_00422 0.34 -1.4 432,598 - CCNA_00422 0.35 -0.1 432,616 + CCNA_00422 0.37 +1.0 432,621 + CCNA_00422 0.38 -0.6 432,626 + CCNA_00422 0.39 -0.5 432,724 + CCNA_00422 0.52 +0.3 432,732 - CCNA_00422 0.53 +0.5 432,760 - CCNA_00422 0.56 -0.7 432,793 + CCNA_00422 0.61 +1.5 432,824 - CCNA_00422 0.65 -2.6 432,903 - CCNA_00422 0.75 -0.6 432,920 - CCNA_00422 0.78 -1.5 432,944 - CCNA_00422 0.81 -0.9 432,963 + CCNA_00422 0.83 +1.8 432,984 + CCNA_00422 0.86 +0.2 433,007 - CCNA_00422 0.89 -0.2 433,039 - -0.6 433,062 + -0.0 433,067 + +0.2 433,070 - +0.3 433,070 - -1.8 433,070 - -0.9 433,070 - -0.1 433,075 - -0.2 433,102 + CCNA_03919 0.15 +0.8 433,116 + CCNA_03919 0.32 -0.4 433,124 - CCNA_03919 0.42 +2.7 433,125 - CCNA_03919 0.43 +1.9 433,136 + CCNA_03919 0.57 +0.3 433,136 + CCNA_03919 0.57 -1.1 433,144 - CCNA_03919 0.67 +3.0 433,144 - CCNA_03919 0.67 +1.9 433,148 - CCNA_03919 0.72 +1.1 433,172 + +0.1 433,205 - -0.1 433,233 - +1.1 433,245 + +1.5 433,253 - -2.4 433,264 + +0.3 433,268 - -0.2 433,268 - -1.3 433,304 + +0.9 433,306 + -1.5 433,306 + -0.9 433,306 + -1.0 433,306 + -1.7 433,306 + +0.3 433,319 + +0.1 433,338 + -0.9 433,338 + -2.2 433,338 + -0.1 433,338 + -1.9 433,346 - -0.9 433,346 - +0.3 433,378 + +0.8 433,390 + +1.5 433,390 + +0.8 433,397 - -0.6 433,406 + -1.7 433,406 + +2.5 433,414 - -2.6 433,445 + -1.5 433,445 + -2.2 433,452 + CCNA_00423 0.10 +0.0 433,453 - CCNA_00423 0.10 -1.6 433,453 - CCNA_00423 0.10 -2.8 433,474 + CCNA_00423 0.15 -0.9 433,474 + CCNA_00423 0.15 +0.0 433,474 + CCNA_00423 0.15 -1.6 433,474 + CCNA_00423 0.15 -2.6 433,474 + CCNA_00423 0.15 +1.8 433,474 + CCNA_00423 0.15 +0.2 433,474 + CCNA_00423 0.15 +1.1 433,482 - CCNA_00423 0.17 +1.6 433,514 + CCNA_00423 0.23 -1.7 433,514 + CCNA_00423 0.23 -0.4 433,522 - CCNA_00423 0.25 +2.2 433,538 + CCNA_00423 0.28 +0.2 433,541 - CCNA_00423 0.29 +0.8 433,546 - CCNA_00423 0.30 +1.9 433,552 - CCNA_00423 0.31 -2.0 433,559 - CCNA_00423 0.33 +0.2 433,560 - CCNA_00423 0.33 -1.2 433,668 + CCNA_00423 0.56 -0.8 433,683 + CCNA_00423 0.59 -0.1 433,696 - CCNA_00423 0.62 -2.2 433,707 + CCNA_00423 0.64 +0.5 433,707 + CCNA_00423 0.64 +0.1 433,741 + CCNA_00423 0.72 -0.3 433,749 - CCNA_00423 0.73 -1.0 433,815 - CCNA_00423 0.87 -0.1 433,839 + +1.3 433,844 + +0.8 433,846 + +1.4 433,851 + +0.6 433,851 + -0.1 433,855 + -0.8 433,859 - -0.9 433,863 - +0.8 433,870 + +0.9 433,873 - +0.1 433,875 + +0.8 433,878 + -1.2 433,899 - -1.3 433,915 - +0.9 433,925 - -3.1 433,926 + -0.2 433,949 + +1.3 433,949 + +1.2 433,949 + +0.6 433,949 + -1.1 433,949 + +0.6 433,949 + -0.4 433,957 - -2.2 433,957 - -0.7 433,957 - -1.0 433,957 - -0.7 433,957 - +0.4 433,957 - -0.2 433,965 - +2.4 433,965 - +1.8 433,965 - -0.4 433,996 + +1.6 433,997 - -0.8 434,033 + +2.3 434,041 - -0.6 434,041 - -0.5 434,041 - -2.9 434,060 + +0.6
Or see this region's nucleotide sequence