Strain Fitness in Caulobacter crescentus NA1000 around CCNA_00400

Experiment: PYE with Polymyxin B sulfate 0.005 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntCCNA_00399 and CCNA_00400 are separated by 44 nucleotidesCCNA_00400 and CCNA_00401 overlap by 4 nucleotidesCCNA_00401 and CCNA_00402 are separated by 3 nucleotides CCNA_00399: CCNA_00399 - zinc metallohydrolase, glyoxalase II family, at 416,919 to 418,016 _00399 CCNA_00400: CCNA_00400 - MarR/EmrR-family transcriptional regulator, at 418,061 to 418,504 _00400 CCNA_00401: CCNA_00401 - enoyl-CoA hydratase, at 418,501 to 419,271 _00401 CCNA_00402: CCNA_00402 - hypothetical protein, at 419,275 to 419,691 _00402 Position (kb) 418 419Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 417.066 kb on + strand, within CCNA_00399at 417.090 kb on + strand, within CCNA_00399at 417.119 kb on + strand, within CCNA_00399at 417.119 kb on + strand, within CCNA_00399at 417.119 kb on + strand, within CCNA_00399at 417.120 kb on + strand, within CCNA_00399at 417.127 kb on - strand, within CCNA_00399at 417.127 kb on - strand, within CCNA_00399at 417.152 kb on - strand, within CCNA_00399at 417.157 kb on + strand, within CCNA_00399at 417.175 kb on - strand, within CCNA_00399at 417.226 kb on + strand, within CCNA_00399at 417.278 kb on + strand, within CCNA_00399at 417.309 kb on + strand, within CCNA_00399at 417.317 kb on - strand, within CCNA_00399at 417.333 kb on - strand, within CCNA_00399at 417.343 kb on - strand, within CCNA_00399at 417.377 kb on - strand, within CCNA_00399at 417.449 kb on + strand, within CCNA_00399at 417.525 kb on - strand, within CCNA_00399at 417.534 kb on + strand, within CCNA_00399at 417.534 kb on + strand, within CCNA_00399at 417.542 kb on - strand, within CCNA_00399at 417.561 kb on + strand, within CCNA_00399at 417.561 kb on + strand, within CCNA_00399at 417.569 kb on - strand, within CCNA_00399at 417.569 kb on - strand, within CCNA_00399at 417.586 kb on - strand, within CCNA_00399at 417.646 kb on - strand, within CCNA_00399at 417.672 kb on + strand, within CCNA_00399at 417.689 kb on + strand, within CCNA_00399at 417.695 kb on + strand, within CCNA_00399at 417.703 kb on - strand, within CCNA_00399at 417.756 kb on + strand, within CCNA_00399at 417.767 kb on - strand, within CCNA_00399at 417.785 kb on - strand, within CCNA_00399at 417.785 kb on - strand, within CCNA_00399at 417.809 kb on - strand, within CCNA_00399at 417.854 kb on - strand, within CCNA_00399at 417.878 kb on + strand, within CCNA_00399at 417.883 kb on + strand, within CCNA_00399at 417.890 kb on + strand, within CCNA_00399at 417.910 kb on + strandat 417.915 kb on + strandat 417.915 kb on + strandat 417.915 kb on + strandat 417.915 kb on + strandat 417.957 kb on - strandat 417.957 kb on - strandat 417.957 kb on - strandat 417.966 kb on + strandat 417.969 kb on + strandat 417.974 kb on - strandat 418.062 kb on + strandat 418.077 kb on + strandat 418.077 kb on + strandat 418.101 kb on + strandat 418.101 kb on + strandat 418.123 kb on + strand, within CCNA_00400at 418.123 kb on + strand, within CCNA_00400at 418.123 kb on + strand, within CCNA_00400at 418.123 kb on + strand, within CCNA_00400at 418.123 kb on + strand, within CCNA_00400at 418.123 kb on + strand, within CCNA_00400at 418.123 kb on + strand, within CCNA_00400at 418.131 kb on - strand, within CCNA_00400at 418.131 kb on - strand, within CCNA_00400at 418.148 kb on + strand, within CCNA_00400at 418.211 kb on + strand, within CCNA_00400at 418.242 kb on + strand, within CCNA_00400at 418.244 kb on - strand, within CCNA_00400at 418.244 kb on - strand, within CCNA_00400at 418.251 kb on + strand, within CCNA_00400at 418.251 kb on + strand, within CCNA_00400at 418.293 kb on + strand, within CCNA_00400at 418.295 kb on - strand, within CCNA_00400at 418.295 kb on - strand, within CCNA_00400at 418.357 kb on + strand, within CCNA_00400at 418.365 kb on - strand, within CCNA_00400at 418.378 kb on + strand, within CCNA_00400at 418.378 kb on + strandat 418.380 kb on - strand, within CCNA_00400at 418.448 kb on + strand, within CCNA_00400at 418.483 kb on + strandat 418.491 kb on - strandat 418.491 kb on - strandat 418.511 kb on - strandat 418.519 kb on + strandat 418.524 kb on + strandat 418.527 kb on - strandat 418.532 kb on + strandat 418.544 kb on + strandat 418.544 kb on + strandat 418.544 kb on + strandat 418.552 kb on - strandat 418.552 kb on - strandat 418.581 kb on + strand, within CCNA_00401at 418.613 kb on + strand, within CCNA_00401at 418.613 kb on + strand, within CCNA_00401at 418.613 kb on - strand, within CCNA_00401at 418.621 kb on - strand, within CCNA_00401at 418.632 kb on + strand, within CCNA_00401at 418.646 kb on + strand, within CCNA_00401at 418.656 kb on + strand, within CCNA_00401at 418.704 kb on - strand, within CCNA_00401at 418.778 kb on - strand, within CCNA_00401at 418.791 kb on + strand, within CCNA_00401at 418.806 kb on + strand, within CCNA_00401at 418.814 kb on - strand, within CCNA_00401at 418.820 kb on + strand, within CCNA_00401at 418.828 kb on - strand, within CCNA_00401at 418.830 kb on - strand, within CCNA_00401at 418.837 kb on + strand, within CCNA_00401at 418.865 kb on + strand, within CCNA_00401at 418.873 kb on - strand, within CCNA_00401at 418.898 kb on + strand, within CCNA_00401at 418.898 kb on + strand, within CCNA_00401at 418.906 kb on - strand, within CCNA_00401at 418.909 kb on + strand, within CCNA_00401at 418.921 kb on + strand, within CCNA_00401at 418.927 kb on + strand, within CCNA_00401at 418.944 kb on - strand, within CCNA_00401at 418.955 kb on - strand, within CCNA_00401at 418.956 kb on + strand, within CCNA_00401at 418.957 kb on + strand, within CCNA_00401at 418.990 kb on + strand, within CCNA_00401at 419.003 kb on - strand, within CCNA_00401at 419.011 kb on - strand, within CCNA_00401at 419.013 kb on + strand, within CCNA_00401at 419.015 kb on + strand, within CCNA_00401at 419.015 kb on + strand, within CCNA_00401at 419.020 kb on + strand, within CCNA_00401at 419.023 kb on - strand, within CCNA_00401at 419.041 kb on - strand, within CCNA_00401at 419.043 kb on - strand, within CCNA_00401at 419.157 kb on + strand, within CCNA_00401at 419.160 kb on + strand, within CCNA_00401at 419.160 kb on + strand, within CCNA_00401at 419.207 kb on - strandat 419.219 kb on - strandat 419.221 kb on + strandat 419.278 kb on + strandat 419.313 kb on + strandat 419.313 kb on + strandat 419.326 kb on + strand, within CCNA_00402at 419.326 kb on + strand, within CCNA_00402at 419.326 kb on + strand, within CCNA_00402at 419.334 kb on - strand, within CCNA_00402at 419.368 kb on - strand, within CCNA_00402at 419.368 kb on - strand, within CCNA_00402at 419.394 kb on + strand, within CCNA_00402at 419.433 kb on - strand, within CCNA_00402at 419.433 kb on - strand, within CCNA_00402at 419.440 kb on + strand, within CCNA_00402at 419.482 kb on + strand, within CCNA_00402

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Per-strain Table

Position Strand Gene LocusTag Fraction PYE with Polymyxin B sulfate 0.005 mg/ml
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417,066 + CCNA_00399 0.13 -1.7
417,090 + CCNA_00399 0.16 +1.0
417,119 + CCNA_00399 0.18 -1.9
417,119 + CCNA_00399 0.18 +0.1
417,119 + CCNA_00399 0.18 +0.1
417,120 + CCNA_00399 0.18 +1.4
417,127 - CCNA_00399 0.19 +1.0
417,127 - CCNA_00399 0.19 +0.6
417,152 - CCNA_00399 0.21 +0.2
417,157 + CCNA_00399 0.22 +1.3
417,175 - CCNA_00399 0.23 -0.8
417,226 + CCNA_00399 0.28 +0.2
417,278 + CCNA_00399 0.33 -1.1
417,309 + CCNA_00399 0.36 -0.1
417,317 - CCNA_00399 0.36 -0.3
417,333 - CCNA_00399 0.38 +0.0
417,343 - CCNA_00399 0.39 -1.5
417,377 - CCNA_00399 0.42 +0.2
417,449 + CCNA_00399 0.48 -2.2
417,525 - CCNA_00399 0.55 -1.6
417,534 + CCNA_00399 0.56 +1.0
417,534 + CCNA_00399 0.56 +0.6
417,542 - CCNA_00399 0.57 -0.3
417,561 + CCNA_00399 0.58 +2.8
417,561 + CCNA_00399 0.58 +0.6
417,569 - CCNA_00399 0.59 -0.3
417,569 - CCNA_00399 0.59 +0.7
417,586 - CCNA_00399 0.61 +1.0
417,646 - CCNA_00399 0.66 -1.0
417,672 + CCNA_00399 0.69 -0.9
417,689 + CCNA_00399 0.70 -1.4
417,695 + CCNA_00399 0.71 +0.8
417,703 - CCNA_00399 0.71 +2.6
417,756 + CCNA_00399 0.76 -1.0
417,767 - CCNA_00399 0.77 -2.1
417,785 - CCNA_00399 0.79 -0.7
417,785 - CCNA_00399 0.79 -0.4
417,809 - CCNA_00399 0.81 -2.0
417,854 - CCNA_00399 0.85 +3.2
417,878 + CCNA_00399 0.87 -0.2
417,883 + CCNA_00399 0.88 -0.8
417,890 + CCNA_00399 0.88 -0.7
417,910 + -0.5
417,915 + -1.5
417,915 + -0.6
417,915 + -2.5
417,915 + +3.1
417,957 - +0.7
417,957 - +0.8
417,957 - -2.4
417,966 + +2.0
417,969 + +0.4
417,974 - +0.8
418,062 + +0.9
418,077 + +2.1
418,077 + +2.5
418,101 + +2.0
418,101 + +2.5
418,123 + CCNA_00400 0.14 +3.4
418,123 + CCNA_00400 0.14 +2.4
418,123 + CCNA_00400 0.14 +2.6
418,123 + CCNA_00400 0.14 +2.7
418,123 + CCNA_00400 0.14 +2.7
418,123 + CCNA_00400 0.14 +2.3
418,123 + CCNA_00400 0.14 +2.8
418,131 - CCNA_00400 0.16 -0.9
418,131 - CCNA_00400 0.16 -1.5
418,148 + CCNA_00400 0.20 +2.4
418,211 + CCNA_00400 0.34 +1.7
418,242 + CCNA_00400 0.41 +1.9
418,244 - CCNA_00400 0.41 -1.9
418,244 - CCNA_00400 0.41 -1.1
418,251 + CCNA_00400 0.43 +3.0
418,251 + CCNA_00400 0.43 +3.4
418,293 + CCNA_00400 0.52 +2.1
418,295 - CCNA_00400 0.53 +1.4
418,295 - CCNA_00400 0.53 -0.4
418,357 + CCNA_00400 0.67 +2.4
418,365 - CCNA_00400 0.68 +0.1
418,378 + CCNA_00400 0.71 +0.9
418,378 + +1.2
418,380 - CCNA_00400 0.72 +0.0
418,448 + CCNA_00400 0.87 +1.7
418,483 + +1.1
418,491 - -0.6
418,491 - -0.6
418,511 - +0.4
418,519 + -0.3
418,524 + -0.8
418,527 - -1.5
418,532 + +0.6
418,544 + +0.9
418,544 + -0.9
418,544 + -1.9
418,552 - +0.6
418,552 - -1.0
418,581 + CCNA_00401 0.10 +0.0
418,613 + CCNA_00401 0.15 -0.8
418,613 + CCNA_00401 0.15 -1.4
418,613 - CCNA_00401 0.15 -0.4
418,621 - CCNA_00401 0.16 -0.9
418,632 + CCNA_00401 0.17 +1.1
418,646 + CCNA_00401 0.19 -0.0
418,656 + CCNA_00401 0.20 +1.3
418,704 - CCNA_00401 0.26 -0.6
418,778 - CCNA_00401 0.36 -2.2
418,791 + CCNA_00401 0.38 -1.6
418,806 + CCNA_00401 0.40 +0.1
418,814 - CCNA_00401 0.41 +0.8
418,820 + CCNA_00401 0.41 -1.2
418,828 - CCNA_00401 0.42 -0.2
418,830 - CCNA_00401 0.43 -1.3
418,837 + CCNA_00401 0.44 +0.2
418,865 + CCNA_00401 0.47 -0.2
418,873 - CCNA_00401 0.48 +0.5
418,898 + CCNA_00401 0.51 +0.9
418,898 + CCNA_00401 0.51 +0.3
418,906 - CCNA_00401 0.53 -0.7
418,909 + CCNA_00401 0.53 +0.1
418,921 + CCNA_00401 0.54 +1.8
418,927 + CCNA_00401 0.55 -0.2
418,944 - CCNA_00401 0.57 -0.1
418,955 - CCNA_00401 0.59 +0.0
418,956 + CCNA_00401 0.59 -0.6
418,957 + CCNA_00401 0.59 -0.5
418,990 + CCNA_00401 0.63 +1.6
419,003 - CCNA_00401 0.65 -0.9
419,011 - CCNA_00401 0.66 -0.7
419,013 + CCNA_00401 0.66 -1.8
419,015 + CCNA_00401 0.67 -1.4
419,015 + CCNA_00401 0.67 -1.8
419,020 + CCNA_00401 0.67 +0.4
419,023 - CCNA_00401 0.68 +1.2
419,041 - CCNA_00401 0.70 +0.0
419,043 - CCNA_00401 0.70 -1.0
419,157 + CCNA_00401 0.85 -1.7
419,160 + CCNA_00401 0.85 -1.0
419,160 + CCNA_00401 0.85 -0.8
419,207 - -1.3
419,219 - +1.0
419,221 + -0.7
419,278 + -0.4
419,313 + -1.0
419,313 + -1.0
419,326 + CCNA_00402 0.12 +0.8
419,326 + CCNA_00402 0.12 +0.3
419,326 + CCNA_00402 0.12 -1.4
419,334 - CCNA_00402 0.14 -0.8
419,368 - CCNA_00402 0.22 -0.2
419,368 - CCNA_00402 0.22 +0.6
419,394 + CCNA_00402 0.29 -3.9
419,433 - CCNA_00402 0.38 +0.6
419,433 - CCNA_00402 0.38 -0.4
419,440 + CCNA_00402 0.40 -1.7
419,482 + CCNA_00402 0.50 -0.8

Or see this region's nucleotide sequence