Experiment: PYE with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00366 and CCNA_00367 overlap by 4 nucleotides CCNA_00367 and CCNA_00368 are separated by 5 nucleotides CCNA_00368 and CCNA_00369 are separated by 149 nucleotides
CCNA_00366: CCNA_00366 - phosphonates transport ATP-binding protein phnC, at 383,009 to 383,803
_00366
CCNA_00367: CCNA_00367 - phosphonates-binding protein, at 383,800 to 384,825
_00367
CCNA_00368: CCNA_00368 - phosphonates transport system permease protein phnE, at 384,831 to 385,667
_00368
CCNA_00369: CCNA_00369 - magnesium and cobalt efflux protein corC-like protein, at 385,817 to 387,130
_00369
Position (kb) 383
384
385 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5 at 382.801 kb on - strand at 382.802 kb on - strand at 382.841 kb on + strand at 382.841 kb on + strand at 382.898 kb on + strand at 382.898 kb on + strand at 382.898 kb on + strand at 382.906 kb on - strand at 382.906 kb on - strand at 382.906 kb on - strand at 382.906 kb on - strand at 382.906 kb on - strand at 382.906 kb on - strand at 382.906 kb on - strand at 382.915 kb on - strand at 382.932 kb on + strand at 382.984 kb on - strand at 382.988 kb on + strand at 383.008 kb on - strand at 383.014 kb on + strand at 383.127 kb on + strand, within CCNA_00366 at 383.145 kb on - strand, within CCNA_00366 at 383.150 kb on + strand, within CCNA_00366 at 383.163 kb on - strand, within CCNA_00366 at 383.178 kb on + strand, within CCNA_00366 at 383.179 kb on - strand, within CCNA_00366 at 383.179 kb on - strand, within CCNA_00366 at 383.186 kb on - strand, within CCNA_00366 at 383.186 kb on - strand, within CCNA_00366 at 383.222 kb on + strand, within CCNA_00366 at 383.222 kb on + strand, within CCNA_00366 at 383.222 kb on + strand, within CCNA_00366 at 383.222 kb on + strand, within CCNA_00366 at 383.230 kb on - strand, within CCNA_00366 at 383.274 kb on - strand, within CCNA_00366 at 383.292 kb on + strand, within CCNA_00366 at 383.300 kb on - strand, within CCNA_00366 at 383.320 kb on + strand, within CCNA_00366 at 383.320 kb on + strand, within CCNA_00366 at 383.353 kb on + strand, within CCNA_00366 at 383.361 kb on - strand, within CCNA_00366 at 383.361 kb on - strand, within CCNA_00366 at 383.363 kb on + strand, within CCNA_00366 at 383.365 kb on - strand, within CCNA_00366 at 383.372 kb on + strand, within CCNA_00366 at 383.406 kb on + strand at 383.417 kb on + strand, within CCNA_00366 at 383.417 kb on + strand, within CCNA_00366 at 383.417 kb on + strand, within CCNA_00366 at 383.417 kb on + strand, within CCNA_00366 at 383.427 kb on + strand, within CCNA_00366 at 383.460 kb on + strand, within CCNA_00366 at 383.491 kb on - strand, within CCNA_00366 at 383.493 kb on + strand, within CCNA_00366 at 383.495 kb on + strand, within CCNA_00366 at 383.520 kb on - strand, within CCNA_00366 at 383.520 kb on - strand, within CCNA_00366 at 383.521 kb on + strand, within CCNA_00366 at 383.546 kb on + strand, within CCNA_00366 at 383.582 kb on + strand, within CCNA_00366 at 383.590 kb on - strand, within CCNA_00366 at 383.605 kb on + strand, within CCNA_00366 at 383.618 kb on + strand, within CCNA_00366 at 383.626 kb on - strand, within CCNA_00366 at 383.636 kb on + strand, within CCNA_00366 at 383.639 kb on - strand, within CCNA_00366 at 383.644 kb on + strand, within CCNA_00366 at 383.644 kb on - strand, within CCNA_00366 at 383.650 kb on + strand, within CCNA_00366 at 383.658 kb on - strand, within CCNA_00366 at 383.658 kb on - strand, within CCNA_00366 at 383.660 kb on - strand, within CCNA_00366 at 383.662 kb on + strand, within CCNA_00366 at 383.667 kb on + strand, within CCNA_00366 at 383.675 kb on - strand, within CCNA_00366 at 383.675 kb on - strand, within CCNA_00366 at 383.686 kb on + strand, within CCNA_00366 at 383.692 kb on - strand, within CCNA_00366 at 383.704 kb on + strand, within CCNA_00366 at 383.705 kb on + strand, within CCNA_00366 at 383.705 kb on + strand, within CCNA_00366 at 383.713 kb on - strand, within CCNA_00366 at 383.760 kb on + strand at 383.787 kb on - strand at 383.829 kb on - strand at 383.839 kb on + strand at 383.845 kb on + strand at 383.845 kb on + strand at 383.853 kb on - strand at 383.853 kb on - strand at 383.853 kb on - strand at 383.864 kb on + strand at 383.869 kb on + strand at 383.869 kb on + strand at 383.877 kb on - strand at 383.922 kb on - strand, within CCNA_00367 at 383.948 kb on - strand, within CCNA_00367 at 383.948 kb on - strand, within CCNA_00367 at 383.959 kb on - strand, within CCNA_00367 at 383.971 kb on - strand, within CCNA_00367 at 383.990 kb on + strand, within CCNA_00367 at 383.990 kb on + strand, within CCNA_00367 at 383.998 kb on - strand, within CCNA_00367 at 384.004 kb on + strand, within CCNA_00367 at 384.033 kb on + strand, within CCNA_00367 at 384.041 kb on - strand, within CCNA_00367 at 384.047 kb on + strand, within CCNA_00367 at 384.061 kb on + strand, within CCNA_00367 at 384.069 kb on - strand, within CCNA_00367 at 384.121 kb on + strand, within CCNA_00367 at 384.165 kb on - strand, within CCNA_00367 at 384.205 kb on + strand, within CCNA_00367 at 384.205 kb on + strand, within CCNA_00367 at 384.205 kb on + strand, within CCNA_00367 at 384.205 kb on + strand, within CCNA_00367 at 384.205 kb on + strand, within CCNA_00367 at 384.205 kb on + strand, within CCNA_00367 at 384.205 kb on + strand, within CCNA_00367 at 384.213 kb on - strand, within CCNA_00367 at 384.213 kb on - strand, within CCNA_00367 at 384.213 kb on - strand, within CCNA_00367 at 384.213 kb on - strand, within CCNA_00367 at 384.213 kb on - strand, within CCNA_00367 at 384.213 kb on - strand, within CCNA_00367 at 384.213 kb on - strand, within CCNA_00367 at 384.214 kb on + strand, within CCNA_00367 at 384.214 kb on + strand, within CCNA_00367 at 384.275 kb on + strand, within CCNA_00367 at 384.283 kb on + strand, within CCNA_00367 at 384.290 kb on + strand, within CCNA_00367 at 384.295 kb on + strand, within CCNA_00367 at 384.298 kb on - strand, within CCNA_00367 at 384.303 kb on - strand, within CCNA_00367 at 384.342 kb on + strand, within CCNA_00367 at 384.354 kb on + strand, within CCNA_00367 at 384.390 kb on + strand, within CCNA_00367 at 384.390 kb on + strand, within CCNA_00367 at 384.403 kb on + strand, within CCNA_00367 at 384.416 kb on - strand, within CCNA_00367 at 384.429 kb on + strand, within CCNA_00367 at 384.429 kb on + strand, within CCNA_00367 at 384.432 kb on - strand, within CCNA_00367 at 384.437 kb on - strand, within CCNA_00367 at 384.437 kb on - strand, within CCNA_00367 at 384.492 kb on + strand, within CCNA_00367 at 384.507 kb on + strand, within CCNA_00367 at 384.507 kb on + strand, within CCNA_00367 at 384.515 kb on - strand, within CCNA_00367 at 384.566 kb on + strand, within CCNA_00367 at 384.574 kb on - strand, within CCNA_00367 at 384.576 kb on + strand, within CCNA_00367 at 384.579 kb on - strand, within CCNA_00367 at 384.606 kb on + strand, within CCNA_00367 at 384.611 kb on + strand, within CCNA_00367 at 384.635 kb on + strand, within CCNA_00367 at 384.635 kb on + strand, within CCNA_00367 at 384.670 kb on - strand, within CCNA_00367 at 384.670 kb on - strand, within CCNA_00367 at 384.708 kb on - strand, within CCNA_00367 at 384.726 kb on + strand at 384.726 kb on + strand at 384.726 kb on + strand at 384.726 kb on + strand at 384.726 kb on + strand at 384.726 kb on + strand at 384.726 kb on + strand at 384.726 kb on + strand at 384.726 kb on + strand at 384.734 kb on - strand at 384.734 kb on - strand at 384.734 kb on - strand at 384.734 kb on - strand at 384.734 kb on - strand at 384.734 kb on - strand at 384.759 kb on - strand at 384.767 kb on + strand at 384.770 kb on - strand at 384.784 kb on + strand at 384.851 kb on + strand at 384.889 kb on + strand at 384.894 kb on + strand at 384.894 kb on + strand at 384.894 kb on + strand at 384.894 kb on + strand at 384.902 kb on - strand at 384.902 kb on - strand at 384.902 kb on - strand at 384.902 kb on - strand at 384.902 kb on - strand at 384.909 kb on + strand at 384.910 kb on + strand at 384.914 kb on + strand at 384.914 kb on + strand at 384.925 kb on + strand, within CCNA_00368 at 384.933 kb on - strand, within CCNA_00368 at 384.933 kb on - strand, within CCNA_00368 at 384.950 kb on + strand, within CCNA_00368 at 384.974 kb on - strand, within CCNA_00368 at 385.010 kb on + strand, within CCNA_00368 at 385.020 kb on + strand, within CCNA_00368 at 385.055 kb on + strand, within CCNA_00368 at 385.077 kb on - strand, within CCNA_00368 at 385.141 kb on - strand, within CCNA_00368 at 385.141 kb on - strand, within CCNA_00368 at 385.182 kb on + strand, within CCNA_00368 at 385.216 kb on + strand, within CCNA_00368 at 385.224 kb on - strand, within CCNA_00368 at 385.229 kb on - strand, within CCNA_00368 at 385.254 kb on + strand, within CCNA_00368 at 385.277 kb on + strand, within CCNA_00368 at 385.279 kb on + strand, within CCNA_00368 at 385.313 kb on + strand, within CCNA_00368 at 385.334 kb on + strand, within CCNA_00368 at 385.415 kb on + strand, within CCNA_00368 at 385.419 kb on - strand, within CCNA_00368 at 385.423 kb on - strand, within CCNA_00368 at 385.424 kb on + strand, within CCNA_00368 at 385.449 kb on + strand, within CCNA_00368 at 385.465 kb on + strand, within CCNA_00368 at 385.465 kb on + strand, within CCNA_00368 at 385.465 kb on + strand, within CCNA_00368 at 385.465 kb on + strand, within CCNA_00368 at 385.470 kb on + strand, within CCNA_00368 at 385.478 kb on - strand, within CCNA_00368 at 385.488 kb on + strand, within CCNA_00368 at 385.509 kb on + strand, within CCNA_00368 at 385.517 kb on - strand, within CCNA_00368 at 385.539 kb on + strand, within CCNA_00368 at 385.604 kb on - strand at 385.643 kb on + strand at 385.645 kb on - strand at 385.656 kb on + strand at 385.656 kb on + strand at 385.656 kb on + strand at 385.656 kb on + strand at 385.661 kb on + strand at 385.661 kb on + strand at 385.664 kb on - strand at 385.664 kb on - strand at 385.664 kb on - strand at 385.666 kb on + strand at 385.666 kb on + strand at 385.666 kb on + strand at 385.666 kb on + strand at 385.666 kb on + strand at 385.666 kb on + strand at 385.714 kb on + strand at 385.721 kb on - strand at 385.721 kb on - strand at 385.757 kb on + strand at 385.760 kb on + strand at 385.798 kb on + strand at 385.814 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Polymyxin B sulfate 0.005 mg/ml remove 382,801 - -1.7 382,802 - +0.3 382,841 + -1.7 382,841 + -0.9 382,898 + -0.3 382,898 + -1.8 382,898 + -0.4 382,906 - -0.2 382,906 - -1.1 382,906 - -1.3 382,906 - -0.3 382,906 - +1.2 382,906 - +0.8 382,906 - -0.4 382,915 - +1.3 382,932 + -0.8 382,984 - +0.1 382,988 + +0.5 383,008 - +0.6 383,014 + +0.7 383,127 + CCNA_00366 0.15 +0.9 383,145 - CCNA_00366 0.17 +0.3 383,150 + CCNA_00366 0.18 +2.6 383,163 - CCNA_00366 0.19 +1.2 383,178 + CCNA_00366 0.21 +1.3 383,179 - CCNA_00366 0.21 -1.1 383,179 - CCNA_00366 0.21 -1.6 383,186 - CCNA_00366 0.22 +0.2 383,186 - CCNA_00366 0.22 +0.6 383,222 + CCNA_00366 0.27 +1.4 383,222 + CCNA_00366 0.27 +3.1 383,222 + CCNA_00366 0.27 +1.4 383,222 + CCNA_00366 0.27 +0.1 383,230 - CCNA_00366 0.28 +0.4 383,274 - CCNA_00366 0.33 +1.0 383,292 + CCNA_00366 0.36 +2.7 383,300 - CCNA_00366 0.37 -0.3 383,320 + CCNA_00366 0.39 +1.5 383,320 + CCNA_00366 0.39 +2.8 383,353 + CCNA_00366 0.43 +0.7 383,361 - CCNA_00366 0.44 +1.4 383,361 - CCNA_00366 0.44 +1.1 383,363 + CCNA_00366 0.45 +1.3 383,365 - CCNA_00366 0.45 -0.4 383,372 + CCNA_00366 0.46 +2.4 383,406 + +3.8 383,417 + CCNA_00366 0.51 +1.4 383,417 + CCNA_00366 0.51 +1.0 383,417 + CCNA_00366 0.51 +1.3 383,417 + CCNA_00366 0.51 +2.5 383,427 + CCNA_00366 0.53 +2.1 383,460 + CCNA_00366 0.57 +2.6 383,491 - CCNA_00366 0.61 -0.0 383,493 + CCNA_00366 0.61 +1.2 383,495 + CCNA_00366 0.61 +1.2 383,520 - CCNA_00366 0.64 -1.7 383,520 - CCNA_00366 0.64 -0.2 383,521 + CCNA_00366 0.64 +0.7 383,546 + CCNA_00366 0.68 +5.0 383,582 + CCNA_00366 0.72 +2.2 383,590 - CCNA_00366 0.73 -1.5 383,605 + CCNA_00366 0.75 +0.5 383,618 + CCNA_00366 0.77 +1.8 383,626 - CCNA_00366 0.78 +1.2 383,636 + CCNA_00366 0.79 +1.9 383,639 - CCNA_00366 0.79 -0.7 383,644 + CCNA_00366 0.80 -0.0 383,644 - CCNA_00366 0.80 +0.2 383,650 + CCNA_00366 0.81 +2.0 383,658 - CCNA_00366 0.82 +1.0 383,658 - CCNA_00366 0.82 +0.6 383,660 - CCNA_00366 0.82 +1.8 383,662 + CCNA_00366 0.82 +1.8 383,667 + CCNA_00366 0.83 +1.6 383,675 - CCNA_00366 0.84 -0.2 383,675 - CCNA_00366 0.84 +0.5 383,686 + CCNA_00366 0.85 +2.0 383,692 - CCNA_00366 0.86 +1.5 383,704 + CCNA_00366 0.87 +2.2 383,705 + CCNA_00366 0.88 +2.0 383,705 + CCNA_00366 0.88 +2.8 383,713 - CCNA_00366 0.89 +2.8 383,760 + +2.2 383,787 - +0.0 383,829 - +0.6 383,839 + +1.5 383,845 + +3.5 383,845 + +1.8 383,853 - +2.8 383,853 - -1.2 383,853 - -0.0 383,864 + +1.2 383,869 + +2.4 383,869 + +1.1 383,877 - -1.8 383,922 - CCNA_00367 0.12 +0.1 383,948 - CCNA_00367 0.14 -1.0 383,948 - CCNA_00367 0.14 +0.3 383,959 - CCNA_00367 0.15 +0.6 383,971 - CCNA_00367 0.17 +4.8 383,990 + CCNA_00367 0.19 +2.5 383,990 + CCNA_00367 0.19 +4.4 383,998 - CCNA_00367 0.19 +1.9 384,004 + CCNA_00367 0.20 +3.7 384,033 + CCNA_00367 0.23 +1.0 384,041 - CCNA_00367 0.23 +2.1 384,047 + CCNA_00367 0.24 +1.2 384,061 + CCNA_00367 0.25 +1.5 384,069 - CCNA_00367 0.26 +1.6 384,121 + CCNA_00367 0.31 +1.7 384,165 - CCNA_00367 0.36 -0.8 384,205 + CCNA_00367 0.39 +1.0 384,205 + CCNA_00367 0.39 +2.7 384,205 + CCNA_00367 0.39 +1.9 384,205 + CCNA_00367 0.39 +2.3 384,205 + CCNA_00367 0.39 +2.0 384,205 + CCNA_00367 0.39 +1.8 384,205 + CCNA_00367 0.39 +2.0 384,213 - CCNA_00367 0.40 -0.6 384,213 - CCNA_00367 0.40 +0.7 384,213 - CCNA_00367 0.40 -1.2 384,213 - CCNA_00367 0.40 +1.3 384,213 - CCNA_00367 0.40 +0.8 384,213 - CCNA_00367 0.40 +0.2 384,213 - CCNA_00367 0.40 -0.2 384,214 + CCNA_00367 0.40 +1.1 384,214 + CCNA_00367 0.40 +1.4 384,275 + CCNA_00367 0.46 +2.8 384,283 + CCNA_00367 0.47 +3.0 384,290 + CCNA_00367 0.48 +1.7 384,295 + CCNA_00367 0.48 +0.7 384,298 - CCNA_00367 0.49 -1.6 384,303 - CCNA_00367 0.49 +0.6 384,342 + CCNA_00367 0.53 +2.2 384,354 + CCNA_00367 0.54 +2.0 384,390 + CCNA_00367 0.58 +1.6 384,390 + CCNA_00367 0.58 +2.2 384,403 + CCNA_00367 0.59 +3.6 384,416 - CCNA_00367 0.60 -0.3 384,429 + CCNA_00367 0.61 +1.0 384,429 + CCNA_00367 0.61 +1.5 384,432 - CCNA_00367 0.62 -0.4 384,437 - CCNA_00367 0.62 -0.1 384,437 - CCNA_00367 0.62 -1.0 384,492 + CCNA_00367 0.67 +2.4 384,507 + CCNA_00367 0.69 +1.9 384,507 + CCNA_00367 0.69 +1.9 384,515 - CCNA_00367 0.70 +1.2 384,566 + CCNA_00367 0.75 +2.2 384,574 - CCNA_00367 0.75 +0.1 384,576 + CCNA_00367 0.76 +2.1 384,579 - CCNA_00367 0.76 +0.8 384,606 + CCNA_00367 0.79 +2.1 384,611 + CCNA_00367 0.79 +2.0 384,635 + CCNA_00367 0.81 +1.3 384,635 + CCNA_00367 0.81 +2.6 384,670 - CCNA_00367 0.85 -1.0 384,670 - CCNA_00367 0.85 +1.1 384,708 - CCNA_00367 0.88 -0.8 384,726 + +2.0 384,726 + +1.7 384,726 + +3.8 384,726 + +2.2 384,726 + +2.7 384,726 + +2.2 384,726 + +2.1 384,726 + +2.7 384,726 + +2.2 384,734 - +1.1 384,734 - -0.4 384,734 - -0.5 384,734 - +0.3 384,734 - -0.4 384,734 - +2.2 384,759 - +1.6 384,767 + -1.5 384,770 - +2.9 384,784 + +1.3 384,851 + +3.1 384,889 + +2.4 384,894 + +1.9 384,894 + +2.0 384,894 + +3.6 384,894 + +2.6 384,902 - -0.3 384,902 - -0.5 384,902 - +4.5 384,902 - -0.1 384,902 - +0.9 384,909 + +1.6 384,910 + +0.5 384,914 + +2.2 384,914 + +3.1 384,925 + CCNA_00368 0.11 +2.1 384,933 - CCNA_00368 0.12 +1.2 384,933 - CCNA_00368 0.12 -0.4 384,950 + CCNA_00368 0.14 +2.9 384,974 - CCNA_00368 0.17 +1.8 385,010 + CCNA_00368 0.21 +2.0 385,020 + CCNA_00368 0.23 +3.0 385,055 + CCNA_00368 0.27 +2.1 385,077 - CCNA_00368 0.29 -0.1 385,141 - CCNA_00368 0.37 +1.9 385,141 - CCNA_00368 0.37 -1.3 385,182 + CCNA_00368 0.42 +2.5 385,216 + CCNA_00368 0.46 +2.8 385,224 - CCNA_00368 0.47 +0.1 385,229 - CCNA_00368 0.48 +0.6 385,254 + CCNA_00368 0.51 +3.2 385,277 + CCNA_00368 0.53 +2.9 385,279 + CCNA_00368 0.54 +2.8 385,313 + CCNA_00368 0.58 +2.6 385,334 + CCNA_00368 0.60 +2.4 385,415 + CCNA_00368 0.70 +2.0 385,419 - CCNA_00368 0.70 +1.6 385,423 - CCNA_00368 0.71 -0.3 385,424 + CCNA_00368 0.71 +2.0 385,449 + CCNA_00368 0.74 +1.5 385,465 + CCNA_00368 0.76 +2.9 385,465 + CCNA_00368 0.76 +1.5 385,465 + CCNA_00368 0.76 +2.5 385,465 + CCNA_00368 0.76 +3.1 385,470 + CCNA_00368 0.76 +2.4 385,478 - CCNA_00368 0.77 -1.3 385,488 + CCNA_00368 0.78 +2.9 385,509 + CCNA_00368 0.81 +2.6 385,517 - CCNA_00368 0.82 -1.3 385,539 + CCNA_00368 0.85 +2.4 385,604 - +0.3 385,643 + +3.0 385,645 - +2.0 385,656 + +3.4 385,656 + +3.0 385,656 + +3.2 385,656 + +3.3 385,661 + +2.5 385,661 + +3.3 385,664 - +0.9 385,664 - +0.8 385,664 - +1.6 385,666 + +2.4 385,666 + +2.7 385,666 + +1.7 385,666 + +2.7 385,666 + +2.6 385,666 + +2.7 385,714 + +0.8 385,721 - -1.9 385,721 - -1.0 385,757 + -0.1 385,760 + +0.1 385,798 + +0.9 385,814 + +1.9
Or see this region's nucleotide sequence