Strain Fitness in Caulobacter crescentus NA1000 around CCNA_00202

Experiment: PYE with Polymyxin B sulfate 0.005 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntCCNA_00200 and CCNA_00201 are separated by 162 nucleotidesCCNA_00201 and CCNA_00202 are separated by 116 nucleotidesCCNA_00202 and CCNA_00203 are separated by 57 nucleotides CCNA_00200: CCNA_00200 - 2OG-Fe(II) oxygenase superfamily protein, at 214,320 to 215,255 _00200 CCNA_00201: CCNA_00201 - outer membrane protein, at 215,418 to 216,098 _00201 CCNA_00202: CCNA_00202 - DesA-family fatty acid desaturase, at 216,215 to 217,270 _00202 CCNA_00203: CCNA_00203 - sterol desaturase family protein, at 217,328 to 218,332 _00203 Position (kb) 216 217 218Strain fitness (log2 ratio) -2 -1 0 1 2 3at 215.272 kb on - strandat 215.303 kb on - strandat 215.376 kb on - strandat 215.382 kb on + strandat 215.409 kb on + strandat 215.454 kb on + strandat 215.454 kb on + strandat 215.459 kb on + strandat 215.462 kb on - strandat 215.473 kb on - strandat 215.499 kb on - strand, within CCNA_00201at 215.501 kb on + strand, within CCNA_00201at 215.559 kb on - strand, within CCNA_00201at 215.559 kb on - strand, within CCNA_00201at 215.563 kb on + strand, within CCNA_00201at 215.563 kb on + strandat 215.568 kb on + strand, within CCNA_00201at 215.568 kb on + strand, within CCNA_00201at 215.568 kb on + strand, within CCNA_00201at 215.568 kb on + strand, within CCNA_00201at 215.568 kb on + strand, within CCNA_00201at 215.568 kb on + strand, within CCNA_00201at 215.568 kb on + strand, within CCNA_00201at 215.571 kb on - strand, within CCNA_00201at 215.576 kb on - strand, within CCNA_00201at 215.576 kb on - strand, within CCNA_00201at 215.576 kb on - strand, within CCNA_00201at 215.576 kb on - strand, within CCNA_00201at 215.576 kb on - strand, within CCNA_00201at 215.576 kb on - strand, within CCNA_00201at 215.576 kb on - strand, within CCNA_00201at 215.576 kb on - strand, within CCNA_00201at 215.576 kb on - strand, within CCNA_00201at 215.576 kb on - strand, within CCNA_00201at 215.581 kb on - strand, within CCNA_00201at 215.590 kb on - strand, within CCNA_00201at 215.595 kb on - strand, within CCNA_00201at 215.624 kb on + strand, within CCNA_00201at 215.624 kb on + strand, within CCNA_00201at 215.656 kb on - strand, within CCNA_00201at 215.704 kb on + strand, within CCNA_00201at 215.712 kb on - strand, within CCNA_00201at 215.713 kb on + strand, within CCNA_00201at 215.721 kb on - strand, within CCNA_00201at 215.721 kb on - strand, within CCNA_00201at 215.756 kb on + strand, within CCNA_00201at 215.769 kb on + strand, within CCNA_00201at 215.769 kb on + strand, within CCNA_00201at 215.838 kb on - strand, within CCNA_00201at 215.859 kb on - strand, within CCNA_00201at 215.877 kb on + strand, within CCNA_00201at 215.889 kb on + strand, within CCNA_00201at 215.899 kb on + strand, within CCNA_00201at 215.907 kb on - strand, within CCNA_00201at 215.920 kb on - strand, within CCNA_00201at 215.944 kb on - strand, within CCNA_00201at 216.012 kb on - strand, within CCNA_00201at 216.049 kb on + strandat 216.054 kb on + strandat 216.081 kb on - strandat 216.087 kb on + strandat 216.095 kb on - strandat 216.101 kb on + strandat 216.135 kb on + strandat 216.135 kb on + strandat 216.135 kb on - strandat 216.141 kb on - strandat 216.143 kb on - strandat 216.187 kb on + strandat 216.195 kb on - strandat 216.195 kb on - strandat 216.197 kb on - strandat 216.197 kb on - strandat 216.197 kb on - strandat 216.253 kb on + strandat 216.254 kb on + strandat 216.255 kb on + strandat 216.261 kb on - strandat 216.263 kb on - strandat 216.263 kb on - strandat 216.274 kb on + strandat 216.275 kb on - strandat 216.293 kb on + strandat 216.298 kb on + strandat 216.298 kb on + strandat 216.298 kb on + strandat 216.298 kb on + strandat 216.298 kb on + strandat 216.306 kb on - strandat 216.306 kb on - strandat 216.329 kb on + strand, within CCNA_00202at 216.337 kb on - strand, within CCNA_00202at 216.337 kb on - strand, within CCNA_00202at 216.362 kb on - strand, within CCNA_00202at 216.372 kb on - strand, within CCNA_00202at 216.423 kb on + strand, within CCNA_00202at 216.486 kb on + strand, within CCNA_00202at 216.530 kb on + strand, within CCNA_00202at 216.532 kb on + strand, within CCNA_00202at 216.539 kb on + strand, within CCNA_00202at 216.551 kb on + strand, within CCNA_00202at 216.559 kb on + strand, within CCNA_00202at 216.584 kb on - strand, within CCNA_00202at 216.603 kb on - strand, within CCNA_00202at 216.607 kb on + strand, within CCNA_00202at 216.615 kb on + strand, within CCNA_00202at 216.621 kb on - strand, within CCNA_00202at 216.663 kb on - strand, within CCNA_00202at 216.677 kb on - strand, within CCNA_00202at 216.785 kb on + strand, within CCNA_00202at 216.785 kb on + strand, within CCNA_00202at 216.785 kb on + strand, within CCNA_00202at 216.785 kb on + strand, within CCNA_00202at 216.785 kb on + strand, within CCNA_00202at 216.785 kb on + strand, within CCNA_00202at 216.785 kb on + strand, within CCNA_00202at 216.793 kb on - strand, within CCNA_00202at 216.793 kb on - strand, within CCNA_00202at 216.805 kb on + strand, within CCNA_00202at 216.812 kb on - strand, within CCNA_00202at 216.840 kb on + strand, within CCNA_00202at 216.848 kb on - strand, within CCNA_00202at 216.848 kb on - strand, within CCNA_00202at 216.893 kb on + strand, within CCNA_00202at 216.901 kb on - strand, within CCNA_00202at 216.912 kb on + strand, within CCNA_00202at 216.956 kb on - strand, within CCNA_00202at 216.956 kb on - strand, within CCNA_00202at 216.964 kb on - strand, within CCNA_00202at 216.987 kb on - strand, within CCNA_00202at 217.064 kb on + strand, within CCNA_00202at 217.074 kb on + strand, within CCNA_00202at 217.074 kb on + strand, within CCNA_00202at 217.082 kb on + strand, within CCNA_00202at 217.120 kb on + strand, within CCNA_00202at 217.129 kb on - strand, within CCNA_00202at 217.166 kb on + strandat 217.202 kb on + strandat 217.214 kb on + strandat 217.216 kb on + strandat 217.255 kb on - strandat 217.260 kb on - strandat 217.260 kb on - strandat 217.354 kb on - strandat 217.354 kb on - strandat 217.354 kb on - strandat 217.354 kb on - strandat 217.396 kb on - strandat 217.398 kb on - strandat 217.419 kb on - strandat 217.420 kb on - strandat 217.429 kb on + strand, within CCNA_00203at 217.446 kb on - strand, within CCNA_00203at 217.446 kb on - strand, within CCNA_00203at 217.472 kb on + strand, within CCNA_00203at 217.480 kb on - strand, within CCNA_00203at 217.480 kb on - strand, within CCNA_00203at 217.480 kb on - strand, within CCNA_00203at 217.480 kb on - strand, within CCNA_00203at 217.480 kb on - strand, within CCNA_00203at 217.503 kb on + strand, within CCNA_00203at 217.544 kb on + strand, within CCNA_00203at 217.552 kb on - strand, within CCNA_00203at 217.564 kb on - strand, within CCNA_00203at 217.564 kb on - strand, within CCNA_00203at 217.566 kb on + strand, within CCNA_00203at 217.566 kb on + strand, within CCNA_00203at 217.571 kb on + strand, within CCNA_00203at 217.584 kb on - strand, within CCNA_00203at 217.602 kb on - strand, within CCNA_00203at 217.610 kb on + strand, within CCNA_00203at 217.610 kb on + strand, within CCNA_00203at 217.620 kb on + strand, within CCNA_00203at 217.632 kb on + strand, within CCNA_00203at 217.638 kb on - strand, within CCNA_00203at 217.656 kb on - strand, within CCNA_00203at 217.668 kb on - strand, within CCNA_00203at 217.676 kb on - strand, within CCNA_00203at 217.715 kb on - strand, within CCNA_00203at 217.768 kb on + strand, within CCNA_00203at 217.798 kb on + strand, within CCNA_00203at 217.798 kb on + strand, within CCNA_00203at 217.798 kb on + strand, within CCNA_00203at 217.834 kb on - strand, within CCNA_00203at 217.834 kb on - strand, within CCNA_00203at 217.834 kb on - strand, within CCNA_00203at 217.834 kb on - strand, within CCNA_00203at 217.834 kb on - strand, within CCNA_00203at 217.838 kb on + strand, within CCNA_00203at 217.878 kb on - strand, within CCNA_00203at 217.886 kb on + strand, within CCNA_00203at 217.937 kb on + strand, within CCNA_00203at 217.937 kb on + strand, within CCNA_00203at 217.937 kb on + strand, within CCNA_00203at 217.945 kb on - strand, within CCNA_00203at 217.950 kb on - strand, within CCNA_00203at 217.962 kb on + strand, within CCNA_00203at 217.968 kb on - strand, within CCNA_00203at 217.995 kb on + strand, within CCNA_00203at 218.018 kb on + strand, within CCNA_00203at 218.018 kb on + strand, within CCNA_00203at 218.019 kb on + strand, within CCNA_00203at 218.027 kb on - strand, within CCNA_00203at 218.044 kb on + strand, within CCNA_00203at 218.080 kb on + strand, within CCNA_00203at 218.085 kb on + strand, within CCNA_00203at 218.088 kb on - strand, within CCNA_00203at 218.140 kb on + strand, within CCNA_00203at 218.146 kb on - strand, within CCNA_00203at 218.173 kb on + strand, within CCNA_00203at 218.237 kb on + strandat 218.267 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction PYE with Polymyxin B sulfate 0.005 mg/ml
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215,272 - -0.5
215,303 - +0.6
215,376 - -0.6
215,382 + +1.9
215,409 + -1.1
215,454 + -1.6
215,454 + +2.2
215,459 + -0.1
215,462 - -1.0
215,473 - +0.5
215,499 - CCNA_00201 0.12 +0.0
215,501 + CCNA_00201 0.12 +0.1
215,559 - CCNA_00201 0.21 -1.4
215,559 - CCNA_00201 0.21 -0.8
215,563 + CCNA_00201 0.21 +1.0
215,563 + +3.2
215,568 + CCNA_00201 0.22 +1.2
215,568 + CCNA_00201 0.22 -2.3
215,568 + CCNA_00201 0.22 +1.2
215,568 + CCNA_00201 0.22 +0.6
215,568 + CCNA_00201 0.22 -1.1
215,568 + CCNA_00201 0.22 -0.1
215,568 + CCNA_00201 0.22 +0.8
215,571 - CCNA_00201 0.22 +0.6
215,576 - CCNA_00201 0.23 +0.8
215,576 - CCNA_00201 0.23 -1.4
215,576 - CCNA_00201 0.23 -0.9
215,576 - CCNA_00201 0.23 +1.0
215,576 - CCNA_00201 0.23 -1.4
215,576 - CCNA_00201 0.23 -0.6
215,576 - CCNA_00201 0.23 -1.5
215,576 - CCNA_00201 0.23 +1.5
215,576 - CCNA_00201 0.23 +2.2
215,576 - CCNA_00201 0.23 -0.8
215,581 - CCNA_00201 0.24 -0.4
215,590 - CCNA_00201 0.25 +0.4
215,595 - CCNA_00201 0.26 -0.1
215,624 + CCNA_00201 0.30 -0.6
215,624 + CCNA_00201 0.30 -0.3
215,656 - CCNA_00201 0.35 -0.3
215,704 + CCNA_00201 0.42 -0.5
215,712 - CCNA_00201 0.43 -1.4
215,713 + CCNA_00201 0.43 +1.0
215,721 - CCNA_00201 0.44 -1.9
215,721 - CCNA_00201 0.44 -0.3
215,756 + CCNA_00201 0.50 -0.4
215,769 + CCNA_00201 0.52 -0.8
215,769 + CCNA_00201 0.52 -0.0
215,838 - CCNA_00201 0.62 -0.1
215,859 - CCNA_00201 0.65 +0.5
215,877 + CCNA_00201 0.67 -0.3
215,889 + CCNA_00201 0.69 -1.3
215,899 + CCNA_00201 0.71 +0.9
215,907 - CCNA_00201 0.72 -2.3
215,920 - CCNA_00201 0.74 -1.5
215,944 - CCNA_00201 0.77 +0.0
216,012 - CCNA_00201 0.87 -0.8
216,049 + +0.1
216,054 + +0.5
216,081 - -0.2
216,087 + +1.0
216,095 - +1.1
216,101 + -1.4
216,135 + -0.6
216,135 + +0.5
216,135 - -0.8
216,141 - -0.7
216,143 - +2.1
216,187 + +0.3
216,195 - +0.7
216,195 - +1.0
216,197 - +0.5
216,197 - -0.7
216,197 - -1.0
216,253 + -0.6
216,254 + -1.1
216,255 + +2.5
216,261 - -0.7
216,263 - -1.2
216,263 - +0.8
216,274 + -0.8
216,275 - -1.7
216,293 + +0.8
216,298 + +0.7
216,298 + -1.4
216,298 + +0.8
216,298 + -0.2
216,298 + -1.2
216,306 - +0.5
216,306 - +3.2
216,329 + CCNA_00202 0.11 +0.5
216,337 - CCNA_00202 0.12 +0.3
216,337 - CCNA_00202 0.12 -0.6
216,362 - CCNA_00202 0.14 +2.7
216,372 - CCNA_00202 0.15 +0.1
216,423 + CCNA_00202 0.20 +0.8
216,486 + CCNA_00202 0.26 +0.6
216,530 + CCNA_00202 0.30 +0.7
216,532 + CCNA_00202 0.30 +2.9
216,539 + CCNA_00202 0.31 -0.4
216,551 + CCNA_00202 0.32 +0.0
216,559 + CCNA_00202 0.33 -0.3
216,584 - CCNA_00202 0.35 -0.5
216,603 - CCNA_00202 0.37 +0.8
216,607 + CCNA_00202 0.37 +2.1
216,615 + CCNA_00202 0.38 +1.4
216,621 - CCNA_00202 0.38 -0.8
216,663 - CCNA_00202 0.42 -0.1
216,677 - CCNA_00202 0.44 +0.8
216,785 + CCNA_00202 0.54 +1.7
216,785 + CCNA_00202 0.54 +0.8
216,785 + CCNA_00202 0.54 +1.6
216,785 + CCNA_00202 0.54 +1.6
216,785 + CCNA_00202 0.54 +1.6
216,785 + CCNA_00202 0.54 -0.4
216,785 + CCNA_00202 0.54 +0.8
216,793 - CCNA_00202 0.55 +1.0
216,793 - CCNA_00202 0.55 -1.2
216,805 + CCNA_00202 0.56 +2.7
216,812 - CCNA_00202 0.57 -0.9
216,840 + CCNA_00202 0.59 +2.7
216,848 - CCNA_00202 0.60 +0.2
216,848 - CCNA_00202 0.60 +1.1
216,893 + CCNA_00202 0.64 +0.3
216,901 - CCNA_00202 0.65 +0.0
216,912 + CCNA_00202 0.66 +1.1
216,956 - CCNA_00202 0.70 -1.1
216,956 - CCNA_00202 0.70 -0.5
216,964 - CCNA_00202 0.71 -0.4
216,987 - CCNA_00202 0.73 -0.5
217,064 + CCNA_00202 0.80 +0.4
217,074 + CCNA_00202 0.81 +2.1
217,074 + CCNA_00202 0.81 +1.1
217,082 + CCNA_00202 0.82 +2.3
217,120 + CCNA_00202 0.86 +2.4
217,129 - CCNA_00202 0.87 -0.6
217,166 + +1.9
217,202 + +1.3
217,214 + +0.4
217,216 + +2.6
217,255 - +1.2
217,260 - +0.5
217,260 - +0.9
217,354 - -0.9
217,354 - +2.8
217,354 - -1.2
217,354 - -0.4
217,396 - +0.2
217,398 - -0.8
217,419 - +0.5
217,420 - -2.3
217,429 + CCNA_00203 0.10 +0.1
217,446 - CCNA_00203 0.12 -0.8
217,446 - CCNA_00203 0.12 -0.8
217,472 + CCNA_00203 0.14 +0.2
217,480 - CCNA_00203 0.15 -1.5
217,480 - CCNA_00203 0.15 +0.3
217,480 - CCNA_00203 0.15 +3.2
217,480 - CCNA_00203 0.15 -0.3
217,480 - CCNA_00203 0.15 -0.1
217,503 + CCNA_00203 0.17 +0.1
217,544 + CCNA_00203 0.21 +0.1
217,552 - CCNA_00203 0.22 +1.2
217,564 - CCNA_00203 0.23 +2.4
217,564 - CCNA_00203 0.23 -1.5
217,566 + CCNA_00203 0.24 -2.4
217,566 + CCNA_00203 0.24 -0.6
217,571 + CCNA_00203 0.24 -2.5
217,584 - CCNA_00203 0.25 -0.9
217,602 - CCNA_00203 0.27 +0.4
217,610 + CCNA_00203 0.28 +1.0
217,610 + CCNA_00203 0.28 +0.1
217,620 + CCNA_00203 0.29 -0.7
217,632 + CCNA_00203 0.30 -1.1
217,638 - CCNA_00203 0.31 +2.0
217,656 - CCNA_00203 0.33 -0.1
217,668 - CCNA_00203 0.34 -0.2
217,676 - CCNA_00203 0.35 +0.1
217,715 - CCNA_00203 0.39 -0.4
217,768 + CCNA_00203 0.44 -1.1
217,798 + CCNA_00203 0.47 -0.4
217,798 + CCNA_00203 0.47 +0.0
217,798 + CCNA_00203 0.47 -1.4
217,834 - CCNA_00203 0.50 +1.7
217,834 - CCNA_00203 0.50 +0.5
217,834 - CCNA_00203 0.50 -0.7
217,834 - CCNA_00203 0.50 -0.4
217,834 - CCNA_00203 0.50 +0.6
217,838 + CCNA_00203 0.51 +1.6
217,878 - CCNA_00203 0.55 -0.3
217,886 + CCNA_00203 0.56 -2.7
217,937 + CCNA_00203 0.61 +0.2
217,937 + CCNA_00203 0.61 +1.2
217,937 + CCNA_00203 0.61 -2.3
217,945 - CCNA_00203 0.61 +0.8
217,950 - CCNA_00203 0.62 +0.5
217,962 + CCNA_00203 0.63 -0.6
217,968 - CCNA_00203 0.64 -1.1
217,995 + CCNA_00203 0.66 -0.9
218,018 + CCNA_00203 0.69 +0.6
218,018 + CCNA_00203 0.69 -1.6
218,019 + CCNA_00203 0.69 -0.4
218,027 - CCNA_00203 0.70 -2.7
218,044 + CCNA_00203 0.71 +0.1
218,080 + CCNA_00203 0.75 -1.7
218,085 + CCNA_00203 0.75 -2.1
218,088 - CCNA_00203 0.76 +1.8
218,140 + CCNA_00203 0.81 +0.6
218,146 - CCNA_00203 0.81 -0.3
218,173 + CCNA_00203 0.84 -1.8
218,237 + -0.8
218,267 - -0.8

Or see this region's nucleotide sequence