Experiment: Paraquat dichloride 0.3 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa1544 and SMa1545 overlap by 1 nucleotides SMa1545 and SMa1547 are separated by 294 nucleotides SMa1547 and SMa1548 are separated by 141 nucleotides
SMa1544: SMa1544 - NuoK2 NADH:quinone oxidoreductase subunit 11 (chain K), at 852,312 to 852,614
SMa1544
SMa1545: SMa1545 - NuoJ2 NADH:quinone oxidoreductase subunit 6 (chain J), at 852,614 to 853,114
SMa1545
SMa1547: SMa1547 - protein-L-isoaspartate(D-aspartate) O-methyltransferase (PCMT), at 853,409 to 854,080
SMa1547
SMa1548: SMa1548 - Diguanylate cyclase/phosphodiesterase, at 854,222 to 857,437
SMa1548
Position (kb) 853
854
855 Strain fitness (log2 ratio) -2
-1
0
1
2 at 852.457 kb on + strand, within SMa1544 at 852.458 kb on - strand, within SMa1544 at 852.460 kb on + strand, within SMa1544 at 852.461 kb on - strand, within SMa1544 at 852.461 kb on - strand, within SMa1544 at 852.936 kb on + strand, within SMa1545 at 852.936 kb on + strand, within SMa1545 at 852.936 kb on + strand, within SMa1545 at 852.937 kb on - strand, within SMa1545 at 852.937 kb on - strand, within SMa1545 at 852.980 kb on - strand, within SMa1545 at 853.168 kb on + strand at 853.194 kb on + strand at 853.194 kb on + strand at 853.194 kb on + strand at 853.217 kb on + strand at 853.217 kb on + strand at 853.277 kb on + strand at 853.278 kb on - strand at 853.278 kb on - strand at 853.337 kb on + strand at 853.338 kb on - strand at 853.338 kb on - strand at 853.359 kb on - strand at 853.476 kb on - strand at 853.624 kb on + strand, within SMa1547 at 853.828 kb on + strand, within SMa1547 at 853.828 kb on + strand, within SMa1547 at 853.828 kb on + strand, within SMa1547 at 853.828 kb on + strand, within SMa1547 at 853.829 kb on - strand, within SMa1547 at 853.829 kb on - strand, within SMa1547 at 853.829 kb on - strand, within SMa1547 at 854.078 kb on + strand at 854.078 kb on + strand at 854.111 kb on + strand at 854.111 kb on + strand at 854.111 kb on + strand at 854.111 kb on + strand at 854.111 kb on + strand at 854.112 kb on - strand at 854.112 kb on - strand at 854.112 kb on - strand at 854.112 kb on - strand at 854.112 kb on - strand at 854.115 kb on + strand at 854.116 kb on - strand at 854.168 kb on + strand at 854.210 kb on - strand at 854.254 kb on + strand at 854.254 kb on + strand at 854.254 kb on + strand at 854.254 kb on + strand at 854.254 kb on + strand at 854.254 kb on + strand at 854.255 kb on - strand at 854.281 kb on + strand at 854.281 kb on + strand at 854.281 kb on + strand at 854.309 kb on - strand at 854.309 kb on - strand at 854.409 kb on + strand at 854.409 kb on + strand at 854.409 kb on + strand at 854.410 kb on - strand at 854.410 kb on - strand at 854.410 kb on - strand at 854.410 kb on - strand at 854.457 kb on + strand at 854.458 kb on - strand at 854.603 kb on - strand, within SMa1548 at 854.654 kb on + strand, within SMa1548 at 854.873 kb on + strand, within SMa1548 at 854.874 kb on - strand, within SMa1548 at 854.874 kb on - strand, within SMa1548 at 854.903 kb on - strand, within SMa1548 at 854.982 kb on + strand, within SMa1548
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.3 mg/ml remove 852,457 + SMa1544 0.48 +0.1 852,458 - SMa1544 0.48 +0.2 852,460 + SMa1544 0.49 -0.8 852,461 - SMa1544 0.49 +0.5 852,461 - SMa1544 0.49 -1.0 852,936 + SMa1545 0.64 +0.6 852,936 + SMa1545 0.64 +0.2 852,936 + SMa1545 0.64 +1.4 852,937 - SMa1545 0.64 -0.2 852,937 - SMa1545 0.64 -0.9 852,980 - SMa1545 0.73 -1.7 853,168 + -0.6 853,194 + -1.9 853,194 + -0.2 853,194 + +0.7 853,217 + -0.6 853,217 + +1.3 853,277 + +0.8 853,278 - -1.3 853,278 - -0.8 853,337 + -1.2 853,338 - -0.1 853,338 - -0.6 853,359 - +0.8 853,476 - +0.8 853,624 + SMa1547 0.32 -1.7 853,828 + SMa1547 0.62 -1.4 853,828 + SMa1547 0.62 -0.2 853,828 + SMa1547 0.62 -1.5 853,828 + SMa1547 0.62 -0.5 853,829 - SMa1547 0.62 -2.0 853,829 - SMa1547 0.62 -0.2 853,829 - SMa1547 0.62 -1.9 854,078 + -0.0 854,078 + -0.9 854,111 + +0.3 854,111 + -0.2 854,111 + -0.5 854,111 + +1.7 854,111 + -1.8 854,112 - -0.8 854,112 - -1.1 854,112 - -0.1 854,112 - -2.6 854,112 - -0.5 854,115 + +1.9 854,116 - +0.7 854,168 + +0.1 854,210 - -0.6 854,254 + -0.3 854,254 + -1.8 854,254 + -1.4 854,254 + +0.3 854,254 + +1.0 854,254 + +0.8 854,255 - -0.2 854,281 + +0.2 854,281 + -1.7 854,281 + +2.1 854,309 - +0.8 854,309 - +1.8 854,409 + +1.4 854,409 + -2.4 854,409 + +0.0 854,410 - +1.1 854,410 - +0.4 854,410 - -2.4 854,410 - -0.2 854,457 + +1.2 854,458 - -0.1 854,603 - SMa1548 0.12 -0.2 854,654 + SMa1548 0.13 +1.8 854,873 + SMa1548 0.20 +1.1 854,874 - SMa1548 0.20 +1.4 854,874 - SMa1548 0.20 -0.4 854,903 - SMa1548 0.21 +0.0 854,982 + SMa1548 0.24 -1.0
Or see this region's nucleotide sequence