Experiment: Paraquat dichloride 0.3 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa1236 and SMa1239 overlap by 26 nucleotides SMa1239 and SMa1240 overlap by 8 nucleotides SMa1240 and SMa1241 are separated by 7 nucleotides SMa1241 and SMa1243 are separated by 259 nucleotides
SMa1236: SMa1236 - nitrate reductase catalytic subunit, at 677,198 to 679,702
SMa1236
SMa1239: SMa1239 - NapD component of periplasmic nitrate reductase, at 679,677 to 679,964
SMa1239
SMa1240: SMa1240 - NapF ferredoxin component of periplasmic nitrate reductase, at 679,957 to 680,457
SMa1240
SMa1241: SMa1241 - NapE component of periplasmic nitrate reductase, at 680,465 to 680,650
SMa1241
SMa1243: SMa1243 - Azu1 pseudoazurin, at 680,910 to 681,353
SMa1243
Position (kb) 679
680
681 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 679.092 kb on + strand, within SMa1236 at 679.092 kb on + strand, within SMa1236 at 679.093 kb on - strand, within SMa1236 at 679.137 kb on + strand, within SMa1236 at 679.137 kb on + strand, within SMa1236 at 679.138 kb on - strand, within SMa1236 at 679.416 kb on + strand, within SMa1236 at 679.416 kb on + strand, within SMa1236 at 679.416 kb on + strand, within SMa1236 at 679.417 kb on - strand, within SMa1236 at 679.437 kb on + strand, within SMa1236 at 679.575 kb on - strand at 679.639 kb on + strand at 679.824 kb on + strand, within SMa1239 at 679.825 kb on - strand, within SMa1239 at 679.937 kb on + strand at 679.942 kb on + strand at 679.942 kb on + strand at 679.942 kb on + strand at 680.055 kb on + strand, within SMa1240 at 680.731 kb on - strand at 680.911 kb on + strand at 681.092 kb on + strand at 681.094 kb on + strand, within SMa1243 at 681.094 kb on + strand, within SMa1243 at 681.095 kb on - strand, within SMa1243 at 681.164 kb on + strand, within SMa1243 at 681.164 kb on + strand, within SMa1243 at 681.165 kb on - strand, within SMa1243 at 681.165 kb on - strand, within SMa1243 at 681.406 kb on - strand at 681.411 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.3 mg/ml remove 679,092 + SMa1236 0.76 +1.6 679,092 + SMa1236 0.76 -0.1 679,093 - SMa1236 0.76 +0.1 679,137 + SMa1236 0.77 +0.3 679,137 + SMa1236 0.77 -0.2 679,138 - SMa1236 0.77 -1.2 679,416 + SMa1236 0.89 +2.6 679,416 + SMa1236 0.89 -1.8 679,416 + SMa1236 0.89 +0.6 679,417 - SMa1236 0.89 +0.6 679,437 + SMa1236 0.89 +0.7 679,575 - -1.9 679,639 + +1.1 679,824 + SMa1239 0.51 +0.2 679,825 - SMa1239 0.51 +2.3 679,937 + +0.3 679,942 + +0.1 679,942 + -1.4 679,942 + -0.1 680,055 + SMa1240 0.20 +1.5 680,731 - +1.6 680,911 + -3.5 681,092 + -1.0 681,094 + SMa1243 0.41 -1.1 681,094 + SMa1243 0.41 +0.5 681,095 - SMa1243 0.42 -0.5 681,164 + SMa1243 0.57 +1.3 681,164 + SMa1243 0.57 +0.2 681,165 - SMa1243 0.57 -2.0 681,165 - SMa1243 0.57 -1.5 681,406 - -1.1 681,411 - -0.9
Or see this region's nucleotide sequence