Experiment: Paraquat dichloride 0.3 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b21702 and SM_b21701 overlap by 123 nucleotides SM_b21701 and SM_b20783 overlap by 73 nucleotides SM_b20783 and SM_b20784 are separated by 172 nucleotides SM_b20784 and SM_b20785 overlap by 20 nucleotides SM_b20785 and SM_b20786 overlap by 4 nucleotides
SM_b21702: SM_b21702 - transposase of insertion sequence ISRm2011-2, orfB N-terminus protein, at 1,576,466 to 1,576,606
_b21702
SM_b21701: SM_b21701 - hypothetical protein, at 1,576,484 to 1,576,621
_b21701
SM_b20783: SM_b20783 - transposase of insertion sequence ISRm2011-2, orfA protein, at 1,576,549 to 1,576,956
_b20783
SM_b20784: SM_b20784 - branched-chain amino acid ABC transporter ATP-binding protein, at 1,577,129 to 1,577,845
_b20784
SM_b20785: SM_b20785 - branched-chain amino acid ABC transporter ATP-binding protein, at 1,577,826 to 1,578,590
_b20785
SM_b20786: SM_b20786 - branched-chain amino acid ABC transporter permease, at 1,578,587 to 1,579,588
_b20786
Position (kb)
1577
1578 Strain fitness (log2 ratio)
-1
0
1 at 1577.416 kb on + strand, within SM_b20784 at 1577.496 kb on + strand, within SM_b20784 at 1577.496 kb on + strand, within SM_b20784 at 1577.497 kb on - strand, within SM_b20784 at 1577.497 kb on - strand, within SM_b20784 at 1577.674 kb on - strand, within SM_b20784 at 1577.674 kb on - strand, within SM_b20784 at 1577.852 kb on - strand at 1577.863 kb on + strand at 1577.864 kb on - strand at 1578.408 kb on + strand, within SM_b20785
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.3 mg/ml remove 1,577,416 + SM_b20784 0.40 -0.3 1,577,496 + SM_b20784 0.51 -0.1 1,577,496 + SM_b20784 0.51 +0.2 1,577,497 - SM_b20784 0.51 -1.4 1,577,497 - SM_b20784 0.51 -0.1 1,577,674 - SM_b20784 0.76 -1.2 1,577,674 - SM_b20784 0.76 -0.6 1,577,852 - -1.1 1,577,863 + -1.3 1,577,864 - -0.5 1,578,408 + SM_b20785 0.76 +0.4
Or see this region's nucleotide sequence