Experiment: CPG
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS13160 and RALBFv3_RS13165 are separated by 73 nucleotides RALBFv3_RS13165 and RALBFv3_RS13170 are separated by 41 nucleotides RALBFv3_RS13170 and RALBFv3_RS13175 are separated by 7 nucleotides RALBFv3_RS13175 and RALBFv3_RS13180 are separated by 44 nucleotides
RALBFv3_RS13160: RALBFv3_RS13160 - DUF3999 domain-containing protein, at 2,886,799 to 2,888,151
_RS13160
RALBFv3_RS13165: RALBFv3_RS13165 - membrane protein, at 2,888,225 to 2,888,608
_RS13165
RALBFv3_RS13170: RALBFv3_RS13170 - RNA-binding protein, at 2,888,650 to 2,888,871
_RS13170
RALBFv3_RS13175: RALBFv3_RS13175 - polyisoprenoid-binding protein, at 2,888,879 to 2,889,448
_RS13175
RALBFv3_RS13180: RALBFv3_RS13180 - glutamine--tRNA ligase, at 2,889,493 to 2,891,235
_RS13180
Position (kb)
2888
2889
2890 Strain fitness (log2 ratio)
-1
0
1 at 2888.150 kb on - strand at 2888.332 kb on - strand, within RALBFv3_RS13165 at 2888.563 kb on - strand, within RALBFv3_RS13165 at 2888.564 kb on - strand, within RALBFv3_RS13165 at 2888.837 kb on + strand, within RALBFv3_RS13170 at 2888.838 kb on - strand, within RALBFv3_RS13170 at 2889.032 kb on - strand, within RALBFv3_RS13175 at 2889.494 kb on + strand at 2889.494 kb on + strand at 2889.494 kb on + strand at 2889.494 kb on + strand at 2889.494 kb on + strand at 2889.494 kb on + strand at 2889.495 kb on - strand at 2889.495 kb on - strand at 2889.495 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction CPG remove 2,888,150 - +0.0 2,888,332 - RALBFv3_RS13165 0.28 -0.5 2,888,563 - RALBFv3_RS13165 0.88 +0.6 2,888,564 - RALBFv3_RS13165 0.88 +0.0 2,888,837 + RALBFv3_RS13170 0.84 -0.8 2,888,838 - RALBFv3_RS13170 0.85 +0.5 2,889,032 - RALBFv3_RS13175 0.27 +0.5 2,889,494 + -0.4 2,889,494 + -0.9 2,889,494 + -0.6 2,889,494 + +0.4 2,889,494 + -0.5 2,889,494 + +0.0 2,889,495 - +0.9 2,889,495 - -0.0 2,889,495 - +0.5
Or see this region's nucleotide sequence