Experiment: CPG
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS10365 and RALBFv3_RS10370 are separated by 264 nucleotides RALBFv3_RS10370 and RALBFv3_RS10375 are separated by 28 nucleotides
RALBFv3_RS10365: RALBFv3_RS10365 - methylmalonyl-CoA mutase, at 2,304,091 to 2,307,390
_RS10365
RALBFv3_RS10370: RALBFv3_RS10370 - 3-hydroxybutyryl-CoA dehydrogenase, at 2,307,655 to 2,308,509
_RS10370
RALBFv3_RS10375: RALBFv3_RS10375 - type III secretion system effector protein, at 2,308,538 to 2,310,055
_RS10375
Position (kb)
2307
2308
2309 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2306.657 kb on - strand, within RALBFv3_RS10365 at 2306.658 kb on - strand, within RALBFv3_RS10365 at 2306.690 kb on - strand, within RALBFv3_RS10365 at 2306.690 kb on - strand, within RALBFv3_RS10365 at 2306.779 kb on + strand, within RALBFv3_RS10365 at 2306.813 kb on - strand, within RALBFv3_RS10365 at 2306.813 kb on - strand, within RALBFv3_RS10365 at 2307.384 kb on + strand at 2307.385 kb on - strand at 2307.385 kb on - strand at 2307.385 kb on - strand at 2307.388 kb on + strand at 2307.388 kb on + strand at 2307.388 kb on + strand at 2307.388 kb on + strand at 2307.388 kb on + strand at 2307.388 kb on + strand at 2307.388 kb on + strand at 2307.389 kb on - strand at 2307.389 kb on - strand at 2307.389 kb on - strand at 2307.389 kb on - strand at 2307.444 kb on + strand at 2307.548 kb on + strand at 2307.655 kb on - strand at 2307.883 kb on + strand, within RALBFv3_RS10370 at 2308.397 kb on - strand, within RALBFv3_RS10370 at 2308.420 kb on + strand, within RALBFv3_RS10370 at 2308.463 kb on - strand at 2308.492 kb on + strand at 2308.493 kb on - strand at 2308.493 kb on - strand at 2308.493 kb on - strand at 2308.499 kb on - strand at 2308.507 kb on + strand at 2308.507 kb on + strand at 2308.508 kb on - strand at 2308.508 kb on - strand at 2308.740 kb on + strand, within RALBFv3_RS10375 at 2308.740 kb on + strand, within RALBFv3_RS10375 at 2308.741 kb on - strand, within RALBFv3_RS10375 at 2308.741 kb on - strand, within RALBFv3_RS10375 at 2308.844 kb on - strand, within RALBFv3_RS10375 at 2308.844 kb on - strand, within RALBFv3_RS10375 at 2308.977 kb on + strand, within RALBFv3_RS10375 at 2308.977 kb on + strand, within RALBFv3_RS10375 at 2308.977 kb on + strand, within RALBFv3_RS10375 at 2308.977 kb on + strand, within RALBFv3_RS10375 at 2308.977 kb on + strand, within RALBFv3_RS10375 at 2308.978 kb on - strand, within RALBFv3_RS10375 at 2309.004 kb on + strand, within RALBFv3_RS10375 at 2309.061 kb on + strand, within RALBFv3_RS10375 at 2309.100 kb on + strand, within RALBFv3_RS10375 at 2309.124 kb on + strand, within RALBFv3_RS10375 at 2309.124 kb on + strand, within RALBFv3_RS10375 at 2309.427 kb on + strand, within RALBFv3_RS10375 at 2309.428 kb on - strand, within RALBFv3_RS10375
Per-strain Table
Position Strand Gene LocusTag Fraction CPG remove 2,306,657 - RALBFv3_RS10365 0.78 +0.9 2,306,658 - RALBFv3_RS10365 0.78 +0.2 2,306,690 - RALBFv3_RS10365 0.79 -0.2 2,306,690 - RALBFv3_RS10365 0.79 -1.0 2,306,779 + RALBFv3_RS10365 0.81 -0.2 2,306,813 - RALBFv3_RS10365 0.82 +1.2 2,306,813 - RALBFv3_RS10365 0.82 +0.4 2,307,384 + -0.7 2,307,385 - -0.1 2,307,385 - -1.2 2,307,385 - -1.3 2,307,388 + +1.8 2,307,388 + +0.7 2,307,388 + +0.3 2,307,388 + +0.5 2,307,388 + -0.1 2,307,388 + +0.2 2,307,388 + +0.1 2,307,389 - +0.4 2,307,389 - -1.3 2,307,389 - -1.0 2,307,389 - -1.2 2,307,444 + -0.1 2,307,548 + -0.1 2,307,655 - -0.0 2,307,883 + RALBFv3_RS10370 0.27 +0.7 2,308,397 - RALBFv3_RS10370 0.87 +0.8 2,308,420 + RALBFv3_RS10370 0.89 -0.4 2,308,463 - -0.1 2,308,492 + -0.7 2,308,493 - -0.4 2,308,493 - -1.9 2,308,493 - -0.3 2,308,499 - -0.1 2,308,507 + -0.3 2,308,507 + +0.2 2,308,508 - -0.2 2,308,508 - -0.1 2,308,740 + RALBFv3_RS10375 0.13 +0.3 2,308,740 + RALBFv3_RS10375 0.13 +0.1 2,308,741 - RALBFv3_RS10375 0.13 +0.2 2,308,741 - RALBFv3_RS10375 0.13 +0.8 2,308,844 - RALBFv3_RS10375 0.20 -1.3 2,308,844 - RALBFv3_RS10375 0.20 -0.7 2,308,977 + RALBFv3_RS10375 0.29 -1.0 2,308,977 + RALBFv3_RS10375 0.29 +0.9 2,308,977 + RALBFv3_RS10375 0.29 -0.2 2,308,977 + RALBFv3_RS10375 0.29 +0.0 2,308,977 + RALBFv3_RS10375 0.29 +0.5 2,308,978 - RALBFv3_RS10375 0.29 -0.3 2,309,004 + RALBFv3_RS10375 0.31 -1.0 2,309,061 + RALBFv3_RS10375 0.34 +0.4 2,309,100 + RALBFv3_RS10375 0.37 +0.2 2,309,124 + RALBFv3_RS10375 0.39 -0.2 2,309,124 + RALBFv3_RS10375 0.39 -0.3 2,309,427 + RALBFv3_RS10375 0.59 -1.3 2,309,428 - RALBFv3_RS10375 0.59 +2.6
Or see this region's nucleotide sequence