Experiment: CPG
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS07690 and RALBFv3_RS07695 are separated by 128 nucleotides RALBFv3_RS07695 and RALBFv3_RS07700 are separated by 175 nucleotides RALBFv3_RS07700 and RALBFv3_RS07705 are separated by 52 nucleotides RALBFv3_RS07705 and RALBFv3_RS07710 overlap by 4 nucleotides RALBFv3_RS07710 and RALBFv3_RS07715 overlap by 20 nucleotides
RALBFv3_RS07690: RALBFv3_RS07690 - calcium sensor EFh, at 1,688,508 to 1,689,071
_RS07690
RALBFv3_RS07695: RALBFv3_RS07695 - pilus assembly protein PilZ, at 1,689,200 to 1,689,886
_RS07695
RALBFv3_RS07700: RALBFv3_RS07700 - type II secretion system protein GspG, at 1,690,062 to 1,690,532
_RS07700
RALBFv3_RS07705: RALBFv3_RS07705 - type II secretion system protein GspH, at 1,690,585 to 1,691,082
_RS07705
RALBFv3_RS07710: RALBFv3_RS07710 - type II secretion system protein GspI, at 1,691,079 to 1,691,477
_RS07710
RALBFv3_RS07715: RALBFv3_RS07715 - general secretion pathway protein GspJ, at 1,691,458 to 1,692,186
_RS07715
Position (kb)
1690
1691 Strain fitness (log2 ratio)
-1
0
1 at 1689.082 kb on + strand at 1689.082 kb on + strand at 1689.082 kb on + strand at 1689.083 kb on - strand at 1689.107 kb on - strand at 1689.140 kb on + strand at 1689.171 kb on - strand at 1689.173 kb on + strand at 1689.173 kb on + strand at 1689.174 kb on - strand at 1689.174 kb on - strand at 1689.307 kb on + strand, within RALBFv3_RS07695 at 1689.308 kb on - strand, within RALBFv3_RS07695 at 1689.515 kb on + strand, within RALBFv3_RS07695 at 1689.515 kb on + strand, within RALBFv3_RS07695 at 1689.515 kb on + strand, within RALBFv3_RS07695 at 1689.516 kb on - strand, within RALBFv3_RS07695 at 1690.006 kb on + strand at 1690.006 kb on + strand at 1690.006 kb on + strand at 1690.006 kb on + strand at 1690.006 kb on + strand at 1690.006 kb on + strand at 1690.006 kb on + strand at 1690.007 kb on - strand at 1690.025 kb on + strand at 1690.026 kb on - strand at 1690.026 kb on - strand at 1690.027 kb on + strand at 1690.027 kb on + strand at 1690.276 kb on - strand, within RALBFv3_RS07700 at 1690.381 kb on - strand, within RALBFv3_RS07700 at 1690.419 kb on + strand, within RALBFv3_RS07700 at 1690.425 kb on + strand, within RALBFv3_RS07700 at 1690.425 kb on + strand, within RALBFv3_RS07700 at 1690.426 kb on - strand, within RALBFv3_RS07700 at 1690.426 kb on - strand, within RALBFv3_RS07700 at 1690.573 kb on + strand at 1690.573 kb on + strand at 1690.574 kb on - strand at 1691.283 kb on + strand, within RALBFv3_RS07710 at 1691.401 kb on - strand, within RALBFv3_RS07710 at 1691.401 kb on - strand, within RALBFv3_RS07710 at 1691.401 kb on - strand, within RALBFv3_RS07710
Per-strain Table
Position Strand Gene LocusTag Fraction CPG remove 1,689,082 + +0.2 1,689,082 + -1.7 1,689,082 + +1.0 1,689,083 - -0.8 1,689,107 - +0.4 1,689,140 + -0.6 1,689,171 - +0.7 1,689,173 + +0.4 1,689,173 + -0.1 1,689,174 - +0.7 1,689,174 - -0.2 1,689,307 + RALBFv3_RS07695 0.16 +0.3 1,689,308 - RALBFv3_RS07695 0.16 +1.0 1,689,515 + RALBFv3_RS07695 0.46 +0.1 1,689,515 + RALBFv3_RS07695 0.46 +0.1 1,689,515 + RALBFv3_RS07695 0.46 +0.6 1,689,516 - RALBFv3_RS07695 0.46 +0.3 1,690,006 + +0.1 1,690,006 + -0.5 1,690,006 + +0.7 1,690,006 + +0.1 1,690,006 + -0.2 1,690,006 + -0.1 1,690,006 + +0.7 1,690,007 - +0.4 1,690,025 + +0.9 1,690,026 - +0.3 1,690,026 - +0.1 1,690,027 + +0.6 1,690,027 + -0.5 1,690,276 - RALBFv3_RS07700 0.45 +0.6 1,690,381 - RALBFv3_RS07700 0.68 +0.5 1,690,419 + RALBFv3_RS07700 0.76 +0.6 1,690,425 + RALBFv3_RS07700 0.77 +0.5 1,690,425 + RALBFv3_RS07700 0.77 +0.3 1,690,426 - RALBFv3_RS07700 0.77 +1.1 1,690,426 - RALBFv3_RS07700 0.77 +0.3 1,690,573 + +0.1 1,690,573 + -0.3 1,690,574 - -0.1 1,691,283 + RALBFv3_RS07710 0.51 +0.9 1,691,401 - RALBFv3_RS07710 0.81 +0.1 1,691,401 - RALBFv3_RS07710 0.81 +0.5 1,691,401 - RALBFv3_RS07710 0.81 -1.0
Or see this region's nucleotide sequence