Strain Fitness in Herbaspirillum seropedicae SmR1 around HSERO_RS10635

Experiment: Plant assay with Setaria viridis; 10 days of inoculation with plant; outgrowth in LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntHSERO_RS10630 and HSERO_RS10635 are separated by 109 nucleotidesHSERO_RS10635 and HSERO_RS10640 are separated by 35 nucleotidesHSERO_RS10640 and HSERO_RS10645 are separated by 59 nucleotides HSERO_RS10630: HSERO_RS10630 - LysR family transcriptional regulator, at 2,392,462 to 2,393,436 _RS10630 HSERO_RS10635: HSERO_RS10635 - guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase, at 2,393,546 to 2,395,807 _RS10635 HSERO_RS10640: HSERO_RS10640 - DNA-directed RNA polymerase subunit omega, at 2,395,843 to 2,396,046 _RS10640 HSERO_RS10645: HSERO_RS10645 - guanylate kinase, at 2,396,106 to 2,396,744 _RS10645 Position (kb) 2393 2394 2395 2396Strain fitness (log2 ratio) -1 0 1at 2392.809 kb on + strand, within HSERO_RS10630at 2393.044 kb on - strand, within HSERO_RS10630at 2393.107 kb on - strand, within HSERO_RS10630at 2393.198 kb on - strand, within HSERO_RS10630at 2393.228 kb on - strand, within HSERO_RS10630at 2393.461 kb on - strandat 2395.802 kb on + strandat 2395.810 kb on - strandat 2396.779 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Plant assay with Setaria viridis; 10 days of inoculation with plant; outgrowth in LB
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2,392,809 + HSERO_RS10630 0.36 +0.7
2,393,044 - HSERO_RS10630 0.60 -0.0
2,393,107 - HSERO_RS10630 0.66 +0.2
2,393,198 - HSERO_RS10630 0.75 -0.4
2,393,228 - HSERO_RS10630 0.79 +1.3
2,393,461 - +0.4
2,395,802 + -1.4
2,395,810 - +0.5
2,396,779 - -1.1

Or see this region's nucleotide sequence