Experiment: Nalidixic 0.08 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS22125 and ABZR86_RS22130 are separated by 138 nucleotides ABZR86_RS22130 and ABZR86_RS22135 overlap by 4 nucleotides ABZR86_RS22135 and ABZR86_RS22140 are separated by 3 nucleotides ABZR86_RS22140 and ABZR86_RS22145 are separated by 76 nucleotides
ABZR86_RS22125: ABZR86_RS22125 - NAD(P) transhydrogenase subunit alpha, at 389,449 to 389,754
_RS22125
ABZR86_RS22130: ABZR86_RS22130 - RNA polymerase sigma factor, at 389,893 to 390,480
_RS22130
ABZR86_RS22135: ABZR86_RS22135 - hypothetical protein, at 390,477 to 390,854
_RS22135
ABZR86_RS22140: ABZR86_RS22140 - DUF3106 domain-containing protein, at 390,858 to 391,424
_RS22140
ABZR86_RS22145: ABZR86_RS22145 - NAD(P) transhydrogenase subunit alpha, at 391,501 to 392,601
_RS22145
Position (kb) 390
391 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 389.519 kb on + strand, within ABZR86_RS22125 at 389.519 kb on + strand, within ABZR86_RS22125 at 389.520 kb on - strand, within ABZR86_RS22125 at 389.520 kb on - strand, within ABZR86_RS22125 at 389.599 kb on + strand, within ABZR86_RS22125 at 389.599 kb on + strand, within ABZR86_RS22125 at 389.628 kb on + strand, within ABZR86_RS22125 at 389.629 kb on - strand, within ABZR86_RS22125 at 389.629 kb on - strand, within ABZR86_RS22125 at 389.629 kb on - strand, within ABZR86_RS22125 at 389.729 kb on + strand at 389.729 kb on + strand at 389.729 kb on + strand at 389.729 kb on + strand at 389.729 kb on + strand at 389.729 kb on + strand at 389.730 kb on - strand at 389.730 kb on - strand at 389.730 kb on - strand at 389.730 kb on - strand at 389.730 kb on - strand at 389.792 kb on + strand at 389.793 kb on - strand at 389.793 kb on - strand at 389.793 kb on - strand at 389.793 kb on - strand at 389.793 kb on - strand at 389.793 kb on - strand at 389.793 kb on - strand at 389.793 kb on - strand at 389.793 kb on - strand at 389.793 kb on - strand at 390.292 kb on + strand, within ABZR86_RS22130 at 390.292 kb on + strand, within ABZR86_RS22130 at 390.292 kb on + strand, within ABZR86_RS22130 at 390.293 kb on - strand, within ABZR86_RS22130 at 390.293 kb on - strand, within ABZR86_RS22130 at 390.377 kb on + strand, within ABZR86_RS22130 at 390.810 kb on + strand, within ABZR86_RS22135 at 390.810 kb on + strand, within ABZR86_RS22135 at 390.810 kb on + strand, within ABZR86_RS22135 at 390.811 kb on - strand, within ABZR86_RS22135 at 390.811 kb on - strand, within ABZR86_RS22135 at 390.811 kb on - strand, within ABZR86_RS22135 at 390.873 kb on - strand at 391.430 kb on + strand at 391.430 kb on + strand at 391.430 kb on + strand at 391.430 kb on + strand at 391.430 kb on + strand at 391.431 kb on - strand at 391.431 kb on - strand at 391.444 kb on - strand at 391.444 kb on - strand at 391.498 kb on + strand at 391.498 kb on + strand at 391.498 kb on + strand at 391.498 kb on + strand at 391.499 kb on - strand at 391.499 kb on - strand at 391.499 kb on - strand at 391.613 kb on + strand, within ABZR86_RS22145 at 391.613 kb on + strand, within ABZR86_RS22145 at 391.614 kb on - strand, within ABZR86_RS22145 at 391.628 kb on + strand, within ABZR86_RS22145 at 391.628 kb on + strand, within ABZR86_RS22145 at 391.628 kb on + strand, within ABZR86_RS22145 at 391.628 kb on + strand, within ABZR86_RS22145 at 391.628 kb on + strand, within ABZR86_RS22145 at 391.628 kb on + strand, within ABZR86_RS22145 at 391.628 kb on + strand, within ABZR86_RS22145 at 391.629 kb on - strand, within ABZR86_RS22145 at 391.629 kb on - strand, within ABZR86_RS22145 at 391.629 kb on - strand, within ABZR86_RS22145 at 391.629 kb on - strand, within ABZR86_RS22145 at 391.629 kb on - strand, within ABZR86_RS22145
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.08 mg/ml remove 389,519 + ABZR86_RS22125 0.23 +1.1 389,519 + ABZR86_RS22125 0.23 +1.5 389,520 - ABZR86_RS22125 0.23 -0.1 389,520 - ABZR86_RS22125 0.23 -1.2 389,599 + ABZR86_RS22125 0.49 +0.5 389,599 + ABZR86_RS22125 0.49 +1.2 389,628 + ABZR86_RS22125 0.58 +0.4 389,629 - ABZR86_RS22125 0.59 -0.5 389,629 - ABZR86_RS22125 0.59 -0.5 389,629 - ABZR86_RS22125 0.59 +0.9 389,729 + +1.2 389,729 + -0.6 389,729 + +0.2 389,729 + -0.5 389,729 + +0.1 389,729 + +0.1 389,730 - -0.3 389,730 - +0.3 389,730 - +0.1 389,730 - -0.1 389,730 - -0.4 389,792 + +0.6 389,793 - +1.0 389,793 - +0.0 389,793 - +0.2 389,793 - -0.5 389,793 - -0.0 389,793 - -1.3 389,793 - +1.0 389,793 - +0.5 389,793 - -0.0 389,793 - +0.7 390,292 + ABZR86_RS22130 0.68 -0.9 390,292 + ABZR86_RS22130 0.68 -0.4 390,292 + ABZR86_RS22130 0.68 -1.1 390,293 - ABZR86_RS22130 0.68 +0.1 390,293 - ABZR86_RS22130 0.68 -0.3 390,377 + ABZR86_RS22130 0.82 +0.2 390,810 + ABZR86_RS22135 0.88 -1.8 390,810 + ABZR86_RS22135 0.88 -0.6 390,810 + ABZR86_RS22135 0.88 -0.6 390,811 - ABZR86_RS22135 0.88 -0.9 390,811 - ABZR86_RS22135 0.88 -2.8 390,811 - ABZR86_RS22135 0.88 +0.1 390,873 - -0.4 391,430 + +0.6 391,430 + +0.2 391,430 + -0.2 391,430 + +0.2 391,430 + +0.4 391,431 - -1.8 391,431 - -0.2 391,444 - +0.6 391,444 - -0.6 391,498 + +0.5 391,498 + +0.4 391,498 + +0.5 391,498 + -0.5 391,499 - +2.2 391,499 - +1.7 391,499 - -0.4 391,613 + ABZR86_RS22145 0.10 -0.6 391,613 + ABZR86_RS22145 0.10 +0.7 391,614 - ABZR86_RS22145 0.10 +0.5 391,628 + ABZR86_RS22145 0.12 -0.9 391,628 + ABZR86_RS22145 0.12 +0.0 391,628 + ABZR86_RS22145 0.12 +0.5 391,628 + ABZR86_RS22145 0.12 +0.2 391,628 + ABZR86_RS22145 0.12 -0.3 391,628 + ABZR86_RS22145 0.12 -0.4 391,628 + ABZR86_RS22145 0.12 +0.8 391,629 - ABZR86_RS22145 0.12 -0.5 391,629 - ABZR86_RS22145 0.12 +0.1 391,629 - ABZR86_RS22145 0.12 -0.4 391,629 - ABZR86_RS22145 0.12 -1.6 391,629 - ABZR86_RS22145 0.12 +1.0
Or see this region's nucleotide sequence