Experiment: Nalidixic 0.08 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS22025 and ABZR86_RS22030 are separated by 57 nucleotides ABZR86_RS22030 and ABZR86_RS22035 are separated by 57 nucleotides ABZR86_RS22035 and ABZR86_RS22040 are separated by 59 nucleotides ABZR86_RS22040 and ABZR86_RS22045 overlap by 4 nucleotides
ABZR86_RS22025: ABZR86_RS22025 - ribose-phosphate diphosphokinase, at 367,551 to 368,489
_RS22025
ABZR86_RS22030: ABZR86_RS22030 - tRNA-Gln, at 368,547 to 368,623
_RS22030
ABZR86_RS22035: ABZR86_RS22035 - 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase, at 368,681 to 370,063
_RS22035
ABZR86_RS22040: ABZR86_RS22040 - lipoprotein insertase outer membrane protein LolB, at 370,123 to 370,731
_RS22040
ABZR86_RS22045: ABZR86_RS22045 - tetratricopeptide repeat protein, at 370,728 to 372,464
_RS22045
Position (kb) 368
369
370
371 Strain fitness (log2 ratio) -2
-1
0
1
2 at 369.673 kb on - strand, within ABZR86_RS22035 at 369.958 kb on - strand at 370.026 kb on - strand at 370.057 kb on - strand at 370.057 kb on - strand at 370.107 kb on + strand at 370.124 kb on + strand at 370.124 kb on + strand at 370.125 kb on - strand at 370.933 kb on + strand, within ABZR86_RS22045 at 371.047 kb on + strand, within ABZR86_RS22045 at 371.047 kb on + strand, within ABZR86_RS22045 at 371.048 kb on - strand, within ABZR86_RS22045 at 371.048 kb on - strand, within ABZR86_RS22045
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.08 mg/ml remove 369,673 - ABZR86_RS22035 0.72 -1.2 369,958 - +0.8 370,026 - +0.4 370,057 - -1.0 370,057 - +1.7 370,107 + -0.7 370,124 + -0.1 370,124 + -0.0 370,125 - +1.1 370,933 + ABZR86_RS22045 0.12 -1.9 371,047 + ABZR86_RS22045 0.18 -0.4 371,047 + ABZR86_RS22045 0.18 -0.1 371,048 - ABZR86_RS22045 0.18 -1.3 371,048 - ABZR86_RS22045 0.18 +1.9
Or see this region's nucleotide sequence