Experiment: Nalidixic 0.08 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS21515 and ABZR86_RS21520 are separated by 64 nucleotides ABZR86_RS21520 and ABZR86_RS21525 are separated by 202 nucleotides ABZR86_RS21525 and ABZR86_RS21530 are separated by 31 nucleotides
ABZR86_RS21515: ABZR86_RS21515 - phosphodiester glycosidase family protein, at 268,933 to 269,682
_RS21515
ABZR86_RS21520: ABZR86_RS21520 - penicillin acylase family protein, at 269,747 to 272,167
_RS21520
ABZR86_RS21525: ABZR86_RS21525 - DUF4019 domain-containing protein, at 272,370 to 272,864
_RS21525
ABZR86_RS21530: ABZR86_RS21530 - type II toxin-antitoxin system HipA family toxin, at 272,896 to 274,206
_RS21530
Position (kb) 269
270
271
272
273 Strain fitness (log2 ratio) -2
-1
0
1
2 at 268.864 kb on + strand at 268.864 kb on + strand at 268.864 kb on + strand at 268.865 kb on - strand at 268.865 kb on - strand at 268.865 kb on - strand at 268.913 kb on - strand at 269.087 kb on - strand, within ABZR86_RS21515 at 269.164 kb on + strand, within ABZR86_RS21515 at 269.164 kb on + strand, within ABZR86_RS21515 at 269.164 kb on + strand, within ABZR86_RS21515 at 269.165 kb on - strand, within ABZR86_RS21515 at 269.165 kb on - strand, within ABZR86_RS21515 at 269.194 kb on + strand, within ABZR86_RS21515 at 269.194 kb on + strand, within ABZR86_RS21515 at 269.194 kb on + strand, within ABZR86_RS21515 at 269.195 kb on - strand, within ABZR86_RS21515 at 269.195 kb on - strand, within ABZR86_RS21515 at 269.195 kb on - strand, within ABZR86_RS21515 at 269.195 kb on - strand, within ABZR86_RS21515 at 269.293 kb on + strand, within ABZR86_RS21515 at 269.294 kb on - strand, within ABZR86_RS21515 at 269.514 kb on + strand, within ABZR86_RS21515 at 269.515 kb on - strand, within ABZR86_RS21515 at 269.515 kb on - strand, within ABZR86_RS21515 at 269.563 kb on + strand, within ABZR86_RS21515 at 269.564 kb on - strand, within ABZR86_RS21515 at 269.590 kb on + strand, within ABZR86_RS21515 at 269.590 kb on + strand, within ABZR86_RS21515 at 269.591 kb on - strand, within ABZR86_RS21515 at 269.591 kb on - strand, within ABZR86_RS21515 at 269.591 kb on - strand, within ABZR86_RS21515 at 269.641 kb on + strand at 269.706 kb on + strand at 269.706 kb on + strand at 269.707 kb on - strand at 269.707 kb on - strand at 269.707 kb on - strand at 269.890 kb on - strand at 269.890 kb on - strand at 269.890 kb on - strand at 269.901 kb on + strand at 269.902 kb on - strand at 269.966 kb on + strand at 269.966 kb on + strand at 269.967 kb on - strand at 269.967 kb on - strand at 269.967 kb on - strand at 269.978 kb on + strand at 269.979 kb on - strand at 269.979 kb on - strand at 270.155 kb on - strand, within ABZR86_RS21520 at 270.167 kb on + strand, within ABZR86_RS21520 at 270.167 kb on + strand, within ABZR86_RS21520 at 270.168 kb on - strand, within ABZR86_RS21520 at 270.168 kb on - strand, within ABZR86_RS21520 at 270.221 kb on + strand, within ABZR86_RS21520 at 270.221 kb on + strand, within ABZR86_RS21520 at 270.221 kb on + strand, within ABZR86_RS21520 at 270.221 kb on + strand, within ABZR86_RS21520 at 270.221 kb on + strand, within ABZR86_RS21520 at 270.222 kb on - strand, within ABZR86_RS21520 at 270.222 kb on - strand, within ABZR86_RS21520 at 270.222 kb on - strand, within ABZR86_RS21520 at 270.222 kb on - strand, within ABZR86_RS21520 at 270.290 kb on + strand, within ABZR86_RS21520 at 270.290 kb on + strand, within ABZR86_RS21520 at 270.291 kb on - strand, within ABZR86_RS21520 at 270.556 kb on + strand, within ABZR86_RS21520 at 270.782 kb on + strand, within ABZR86_RS21520 at 270.783 kb on - strand, within ABZR86_RS21520 at 271.247 kb on + strand, within ABZR86_RS21520 at 271.572 kb on - strand, within ABZR86_RS21520 at 271.581 kb on + strand, within ABZR86_RS21520 at 271.581 kb on + strand, within ABZR86_RS21520 at 271.614 kb on + strand, within ABZR86_RS21520 at 271.649 kb on + strand, within ABZR86_RS21520 at 271.650 kb on - strand, within ABZR86_RS21520 at 271.650 kb on - strand, within ABZR86_RS21520 at 272.063 kb on + strand at 272.064 kb on - strand at 272.066 kb on + strand at 272.066 kb on + strand at 272.066 kb on + strand at 272.067 kb on - strand at 272.067 kb on - strand at 272.228 kb on + strand at 272.228 kb on + strand at 272.228 kb on + strand at 272.229 kb on - strand at 272.229 kb on - strand at 272.229 kb on - strand at 272.307 kb on + strand at 272.307 kb on + strand at 272.307 kb on + strand at 272.308 kb on - strand at 272.308 kb on - strand at 272.308 kb on - strand at 272.308 kb on - strand at 272.308 kb on - strand at 272.308 kb on - strand at 272.308 kb on - strand at 272.308 kb on - strand at 272.308 kb on - strand at 272.369 kb on + strand at 272.369 kb on + strand at 272.369 kb on + strand at 272.369 kb on + strand at 272.369 kb on + strand at 272.369 kb on + strand at 272.369 kb on + strand at 272.370 kb on - strand at 272.370 kb on - strand at 272.370 kb on - strand at 272.586 kb on + strand, within ABZR86_RS21525 at 272.587 kb on - strand, within ABZR86_RS21525 at 272.587 kb on - strand, within ABZR86_RS21525 at 272.587 kb on - strand, within ABZR86_RS21525 at 272.782 kb on - strand, within ABZR86_RS21525 at 272.782 kb on - strand, within ABZR86_RS21525 at 272.782 kb on - strand, within ABZR86_RS21525 at 272.782 kb on - strand, within ABZR86_RS21525
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.08 mg/ml remove 268,864 + -1.0 268,864 + -0.8 268,864 + -0.4 268,865 - -1.3 268,865 - +0.6 268,865 - -1.1 268,913 - -0.3 269,087 - ABZR86_RS21515 0.21 -1.9 269,164 + ABZR86_RS21515 0.31 -1.7 269,164 + ABZR86_RS21515 0.31 +0.8 269,164 + ABZR86_RS21515 0.31 -0.9 269,165 - ABZR86_RS21515 0.31 -1.7 269,165 - ABZR86_RS21515 0.31 +0.4 269,194 + ABZR86_RS21515 0.35 +0.1 269,194 + ABZR86_RS21515 0.35 +1.0 269,194 + ABZR86_RS21515 0.35 -0.0 269,195 - ABZR86_RS21515 0.35 -0.2 269,195 - ABZR86_RS21515 0.35 -0.8 269,195 - ABZR86_RS21515 0.35 -1.6 269,195 - ABZR86_RS21515 0.35 +2.3 269,293 + ABZR86_RS21515 0.48 +0.2 269,294 - ABZR86_RS21515 0.48 -0.5 269,514 + ABZR86_RS21515 0.77 -0.2 269,515 - ABZR86_RS21515 0.78 +0.4 269,515 - ABZR86_RS21515 0.78 -0.3 269,563 + ABZR86_RS21515 0.84 -0.7 269,564 - ABZR86_RS21515 0.84 -0.9 269,590 + ABZR86_RS21515 0.88 -0.1 269,590 + ABZR86_RS21515 0.88 +0.7 269,591 - ABZR86_RS21515 0.88 -0.1 269,591 - ABZR86_RS21515 0.88 +0.9 269,591 - ABZR86_RS21515 0.88 +0.2 269,641 + +0.8 269,706 + +0.3 269,706 + +0.3 269,707 - -0.3 269,707 - -1.2 269,707 - -0.3 269,890 - +1.2 269,890 - +1.3 269,890 - +0.5 269,901 + -2.3 269,902 - +0.2 269,966 + -2.3 269,966 + +0.2 269,967 - +0.2 269,967 - +0.1 269,967 - -0.1 269,978 + +0.2 269,979 - -0.3 269,979 - -0.4 270,155 - ABZR86_RS21520 0.17 +0.6 270,167 + ABZR86_RS21520 0.17 -0.5 270,167 + ABZR86_RS21520 0.17 -0.5 270,168 - ABZR86_RS21520 0.17 +0.2 270,168 - ABZR86_RS21520 0.17 +0.4 270,221 + ABZR86_RS21520 0.20 -0.2 270,221 + ABZR86_RS21520 0.20 +0.6 270,221 + ABZR86_RS21520 0.20 -1.2 270,221 + ABZR86_RS21520 0.20 -0.7 270,221 + ABZR86_RS21520 0.20 +0.5 270,222 - ABZR86_RS21520 0.20 +0.8 270,222 - ABZR86_RS21520 0.20 +0.5 270,222 - ABZR86_RS21520 0.20 +0.6 270,222 - ABZR86_RS21520 0.20 +0.9 270,290 + ABZR86_RS21520 0.22 +0.2 270,290 + ABZR86_RS21520 0.22 +0.4 270,291 - ABZR86_RS21520 0.22 +0.2 270,556 + ABZR86_RS21520 0.33 -0.6 270,782 + ABZR86_RS21520 0.43 +1.5 270,783 - ABZR86_RS21520 0.43 -2.1 271,247 + ABZR86_RS21520 0.62 -1.6 271,572 - ABZR86_RS21520 0.75 +0.3 271,581 + ABZR86_RS21520 0.76 -0.9 271,581 + ABZR86_RS21520 0.76 -0.4 271,614 + ABZR86_RS21520 0.77 -1.3 271,649 + ABZR86_RS21520 0.79 +0.2 271,650 - ABZR86_RS21520 0.79 -1.0 271,650 - ABZR86_RS21520 0.79 +0.5 272,063 + -0.1 272,064 - -0.0 272,066 + +0.4 272,066 + +1.4 272,066 + -0.0 272,067 - +1.2 272,067 - -0.7 272,228 + -2.7 272,228 + +0.3 272,228 + +0.3 272,229 - -0.0 272,229 - -0.6 272,229 - +0.4 272,307 + -0.6 272,307 + -1.6 272,307 + -0.4 272,308 - -1.2 272,308 - -0.5 272,308 - -0.3 272,308 - -1.8 272,308 - +0.4 272,308 - -0.6 272,308 - +0.5 272,308 - -0.1 272,308 - -0.5 272,369 + -0.4 272,369 + +1.1 272,369 + -0.4 272,369 + -0.2 272,369 + +0.8 272,369 + +0.4 272,369 + -1.2 272,370 - -0.3 272,370 - +0.4 272,370 - -0.8 272,586 + ABZR86_RS21525 0.44 +0.3 272,587 - ABZR86_RS21525 0.44 -1.9 272,587 - ABZR86_RS21525 0.44 +0.1 272,587 - ABZR86_RS21525 0.44 -2.7 272,782 - ABZR86_RS21525 0.83 -1.3 272,782 - ABZR86_RS21525 0.83 -1.4 272,782 - ABZR86_RS21525 0.83 -0.1 272,782 - ABZR86_RS21525 0.83 +2.5
Or see this region's nucleotide sequence