Experiment: Nalidixic 0.08 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS21245 and ABZR86_RS21250 are separated by 61 nucleotides ABZR86_RS21250 and ABZR86_RS21255 are separated by 22 nucleotides ABZR86_RS21255 and ABZR86_RS21260 are separated by 25 nucleotides ABZR86_RS21260 and ABZR86_RS21265 are separated by 87 nucleotides
ABZR86_RS21245: ABZR86_RS21245 - N-acetylmuramoyl-L-alanine amidase, at 217,786 to 218,361
_RS21245
ABZR86_RS21250: ABZR86_RS21250 - acyl-CoA thioesterase II, at 218,423 to 219,286
_RS21250
ABZR86_RS21255: ABZR86_RS21255 - DUF2007 domain-containing protein, at 219,309 to 219,701
_RS21255
ABZR86_RS21260: ABZR86_RS21260 - arginyltransferase, at 219,727 to 220,506
_RS21260
ABZR86_RS21265: ABZR86_RS21265 - FMN-binding glutamate synthase family protein, at 220,594 to 222,108
_RS21265
Position (kb) 219
220 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 218.380 kb on - strand at 218.380 kb on - strand at 218.381 kb on + strand at 218.381 kb on + strand at 218.382 kb on - strand at 218.382 kb on - strand at 218.402 kb on + strand at 218.612 kb on + strand, within ABZR86_RS21250 at 218.612 kb on + strand, within ABZR86_RS21250 at 218.612 kb on + strand, within ABZR86_RS21250 at 218.612 kb on + strand, within ABZR86_RS21250 at 218.612 kb on + strand, within ABZR86_RS21250 at 218.612 kb on + strand, within ABZR86_RS21250 at 218.612 kb on + strand, within ABZR86_RS21250 at 218.613 kb on - strand, within ABZR86_RS21250 at 218.613 kb on - strand, within ABZR86_RS21250 at 218.613 kb on - strand, within ABZR86_RS21250 at 218.613 kb on - strand, within ABZR86_RS21250 at 218.613 kb on - strand, within ABZR86_RS21250 at 218.652 kb on - strand, within ABZR86_RS21250 at 218.652 kb on - strand, within ABZR86_RS21250 at 218.955 kb on - strand, within ABZR86_RS21250 at 219.026 kb on + strand, within ABZR86_RS21250 at 219.026 kb on + strand, within ABZR86_RS21250 at 219.026 kb on + strand, within ABZR86_RS21250 at 219.027 kb on - strand, within ABZR86_RS21250 at 219.080 kb on + strand, within ABZR86_RS21250 at 219.080 kb on + strand, within ABZR86_RS21250 at 219.080 kb on + strand, within ABZR86_RS21250 at 219.080 kb on + strand, within ABZR86_RS21250 at 219.081 kb on - strand, within ABZR86_RS21250 at 219.081 kb on - strand, within ABZR86_RS21250 at 219.083 kb on + strand, within ABZR86_RS21250 at 219.083 kb on + strand, within ABZR86_RS21250 at 219.083 kb on + strand, within ABZR86_RS21250 at 219.083 kb on + strand, within ABZR86_RS21250 at 219.084 kb on - strand, within ABZR86_RS21250 at 219.165 kb on - strand, within ABZR86_RS21250 at 219.297 kb on + strand at 219.297 kb on + strand at 219.321 kb on + strand at 219.321 kb on + strand at 219.321 kb on + strand at 219.321 kb on + strand at 219.321 kb on + strand at 219.322 kb on - strand at 219.322 kb on - strand at 219.322 kb on - strand at 219.322 kb on - strand at 219.322 kb on - strand at 219.322 kb on - strand at 219.322 kb on - strand at 219.324 kb on - strand at 219.324 kb on - strand at 219.426 kb on + strand, within ABZR86_RS21255 at 219.426 kb on + strand, within ABZR86_RS21255 at 219.427 kb on - strand, within ABZR86_RS21255 at 219.427 kb on - strand, within ABZR86_RS21255 at 219.427 kb on - strand, within ABZR86_RS21255 at 219.504 kb on + strand, within ABZR86_RS21255 at 219.504 kb on + strand, within ABZR86_RS21255 at 219.505 kb on - strand, within ABZR86_RS21255 at 219.505 kb on - strand, within ABZR86_RS21255 at 219.505 kb on - strand, within ABZR86_RS21255 at 219.684 kb on + strand at 219.684 kb on + strand at 219.684 kb on + strand at 219.684 kb on + strand at 219.684 kb on + strand at 219.684 kb on + strand at 219.684 kb on + strand at 219.685 kb on - strand at 219.685 kb on - strand at 219.685 kb on - strand at 219.731 kb on + strand at 219.732 kb on - strand at 219.732 kb on - strand at 219.732 kb on - strand at 219.791 kb on + strand at 219.791 kb on + strand at 219.791 kb on + strand at 219.792 kb on - strand at 219.792 kb on - strand at 219.792 kb on - strand at 219.792 kb on - strand at 219.854 kb on - strand, within ABZR86_RS21260 at 219.854 kb on - strand, within ABZR86_RS21260 at 219.854 kb on - strand, within ABZR86_RS21260 at 219.920 kb on - strand, within ABZR86_RS21260 at 219.920 kb on - strand, within ABZR86_RS21260 at 219.967 kb on + strand, within ABZR86_RS21260 at 219.967 kb on + strand, within ABZR86_RS21260 at 219.967 kb on + strand, within ABZR86_RS21260 at 219.968 kb on - strand, within ABZR86_RS21260 at 219.968 kb on - strand, within ABZR86_RS21260 at 219.968 kb on - strand, within ABZR86_RS21260 at 219.968 kb on - strand, within ABZR86_RS21260 at 219.968 kb on - strand, within ABZR86_RS21260 at 219.976 kb on + strand, within ABZR86_RS21260 at 219.977 kb on - strand, within ABZR86_RS21260 at 220.102 kb on + strand, within ABZR86_RS21260 at 220.103 kb on - strand, within ABZR86_RS21260 at 220.126 kb on + strand, within ABZR86_RS21260 at 220.126 kb on + strand, within ABZR86_RS21260 at 220.126 kb on + strand, within ABZR86_RS21260 at 220.126 kb on + strand, within ABZR86_RS21260 at 220.126 kb on + strand, within ABZR86_RS21260 at 220.126 kb on + strand, within ABZR86_RS21260 at 220.127 kb on - strand, within ABZR86_RS21260 at 220.127 kb on - strand, within ABZR86_RS21260 at 220.135 kb on + strand, within ABZR86_RS21260 at 220.135 kb on + strand, within ABZR86_RS21260 at 220.135 kb on + strand, within ABZR86_RS21260 at 220.136 kb on - strand, within ABZR86_RS21260 at 220.306 kb on + strand, within ABZR86_RS21260 at 220.306 kb on + strand, within ABZR86_RS21260 at 220.306 kb on + strand, within ABZR86_RS21260 at 220.307 kb on - strand, within ABZR86_RS21260 at 220.307 kb on - strand, within ABZR86_RS21260 at 220.405 kb on + strand, within ABZR86_RS21260 at 220.405 kb on + strand, within ABZR86_RS21260 at 220.405 kb on + strand, within ABZR86_RS21260 at 220.406 kb on - strand, within ABZR86_RS21260 at 220.444 kb on + strand at 220.444 kb on + strand at 220.444 kb on + strand at 220.444 kb on + strand at 220.444 kb on + strand at 220.444 kb on + strand at 220.445 kb on - strand at 220.445 kb on - strand at 220.445 kb on - strand at 220.504 kb on + strand at 220.504 kb on + strand at 220.504 kb on + strand at 220.505 kb on - strand at 220.505 kb on - strand at 220.588 kb on + strand at 220.588 kb on + strand at 220.589 kb on - strand at 220.597 kb on + strand at 220.597 kb on + strand at 220.597 kb on + strand at 220.597 kb on + strand at 220.598 kb on - strand at 220.610 kb on + strand at 220.610 kb on + strand at 220.610 kb on + strand at 220.610 kb on + strand at 220.636 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.08 mg/ml remove 218,380 - +0.1 218,380 - -0.3 218,381 + +0.1 218,381 + +0.6 218,382 - -0.0 218,382 - -0.2 218,402 + -0.2 218,612 + ABZR86_RS21250 0.22 +0.3 218,612 + ABZR86_RS21250 0.22 +0.7 218,612 + ABZR86_RS21250 0.22 -0.9 218,612 + ABZR86_RS21250 0.22 -0.3 218,612 + ABZR86_RS21250 0.22 -0.4 218,612 + ABZR86_RS21250 0.22 +0.4 218,612 + ABZR86_RS21250 0.22 +0.4 218,613 - ABZR86_RS21250 0.22 -0.0 218,613 - ABZR86_RS21250 0.22 +0.6 218,613 - ABZR86_RS21250 0.22 +0.0 218,613 - ABZR86_RS21250 0.22 +0.2 218,613 - ABZR86_RS21250 0.22 +0.4 218,652 - ABZR86_RS21250 0.27 +0.1 218,652 - ABZR86_RS21250 0.27 -1.0 218,955 - ABZR86_RS21250 0.62 +0.2 219,026 + ABZR86_RS21250 0.70 +1.5 219,026 + ABZR86_RS21250 0.70 +0.5 219,026 + ABZR86_RS21250 0.70 -0.7 219,027 - ABZR86_RS21250 0.70 -0.5 219,080 + ABZR86_RS21250 0.76 -0.1 219,080 + ABZR86_RS21250 0.76 +1.1 219,080 + ABZR86_RS21250 0.76 -0.1 219,080 + ABZR86_RS21250 0.76 +0.2 219,081 - ABZR86_RS21250 0.76 +0.4 219,081 - ABZR86_RS21250 0.76 +0.3 219,083 + ABZR86_RS21250 0.76 +0.5 219,083 + ABZR86_RS21250 0.76 -0.1 219,083 + ABZR86_RS21250 0.76 -0.1 219,083 + ABZR86_RS21250 0.76 +0.3 219,084 - ABZR86_RS21250 0.77 +1.1 219,165 - ABZR86_RS21250 0.86 +0.1 219,297 + +0.4 219,297 + -0.0 219,321 + -1.0 219,321 + -0.4 219,321 + -0.8 219,321 + -0.1 219,321 + -1.4 219,322 - +0.4 219,322 - -0.7 219,322 - -1.4 219,322 - -1.5 219,322 - -0.1 219,322 - -1.0 219,322 - -0.9 219,324 - -0.7 219,324 - -2.3 219,426 + ABZR86_RS21255 0.30 -0.5 219,426 + ABZR86_RS21255 0.30 -3.2 219,427 - ABZR86_RS21255 0.30 -0.2 219,427 - ABZR86_RS21255 0.30 -1.9 219,427 - ABZR86_RS21255 0.30 +0.8 219,504 + ABZR86_RS21255 0.50 -0.4 219,504 + ABZR86_RS21255 0.50 -1.5 219,505 - ABZR86_RS21255 0.50 -1.0 219,505 - ABZR86_RS21255 0.50 -0.8 219,505 - ABZR86_RS21255 0.50 -0.9 219,684 + +0.7 219,684 + -1.2 219,684 + +1.1 219,684 + -0.5 219,684 + +1.0 219,684 + -0.3 219,684 + +0.2 219,685 - -0.2 219,685 - +0.7 219,685 - +0.8 219,731 + +0.4 219,732 - +0.4 219,732 - +0.2 219,732 - +0.2 219,791 + -2.5 219,791 + -0.1 219,791 + +0.3 219,792 - +0.5 219,792 - +0.4 219,792 - -0.5 219,792 - +0.7 219,854 - ABZR86_RS21260 0.16 -1.1 219,854 - ABZR86_RS21260 0.16 +0.4 219,854 - ABZR86_RS21260 0.16 +0.1 219,920 - ABZR86_RS21260 0.25 -0.8 219,920 - ABZR86_RS21260 0.25 -0.3 219,967 + ABZR86_RS21260 0.31 +0.3 219,967 + ABZR86_RS21260 0.31 -0.4 219,967 + ABZR86_RS21260 0.31 +1.0 219,968 - ABZR86_RS21260 0.31 -0.2 219,968 - ABZR86_RS21260 0.31 -0.2 219,968 - ABZR86_RS21260 0.31 -0.9 219,968 - ABZR86_RS21260 0.31 -0.5 219,968 - ABZR86_RS21260 0.31 +2.4 219,976 + ABZR86_RS21260 0.32 -0.5 219,977 - ABZR86_RS21260 0.32 +0.7 220,102 + ABZR86_RS21260 0.48 +1.9 220,103 - ABZR86_RS21260 0.48 +0.1 220,126 + ABZR86_RS21260 0.51 +0.6 220,126 + ABZR86_RS21260 0.51 +0.3 220,126 + ABZR86_RS21260 0.51 +1.4 220,126 + ABZR86_RS21260 0.51 -0.5 220,126 + ABZR86_RS21260 0.51 +1.2 220,126 + ABZR86_RS21260 0.51 +0.4 220,127 - ABZR86_RS21260 0.51 -1.0 220,127 - ABZR86_RS21260 0.51 +0.5 220,135 + ABZR86_RS21260 0.52 -0.5 220,135 + ABZR86_RS21260 0.52 +0.5 220,135 + ABZR86_RS21260 0.52 -0.5 220,136 - ABZR86_RS21260 0.52 -0.5 220,306 + ABZR86_RS21260 0.74 -0.9 220,306 + ABZR86_RS21260 0.74 +0.4 220,306 + ABZR86_RS21260 0.74 +0.6 220,307 - ABZR86_RS21260 0.74 -0.3 220,307 - ABZR86_RS21260 0.74 +1.2 220,405 + ABZR86_RS21260 0.87 -0.6 220,405 + ABZR86_RS21260 0.87 -0.9 220,405 + ABZR86_RS21260 0.87 -0.7 220,406 - ABZR86_RS21260 0.87 +0.7 220,444 + -0.3 220,444 + +0.2 220,444 + -0.2 220,444 + +0.6 220,444 + +0.5 220,444 + +0.2 220,445 - +0.5 220,445 - +0.5 220,445 - -2.1 220,504 + -1.1 220,504 + -0.1 220,504 + +0.1 220,505 - +0.5 220,505 - -0.7 220,588 + -0.3 220,588 + +0.3 220,589 - -0.0 220,597 + -0.7 220,597 + +0.3 220,597 + +0.4 220,597 + -0.6 220,598 - +0.2 220,610 + -0.4 220,610 + +1.0 220,610 + +0.0 220,610 + +0.6 220,636 + -1.7
Or see this region's nucleotide sequence