Experiment: Nalidixic 0.08 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS20365 and ABZR86_RS20370 are separated by 88 nucleotides ABZR86_RS20370 and ABZR86_RS20375 are separated by 20 nucleotides ABZR86_RS20375 and ABZR86_RS20380 overlap by 17 nucleotides ABZR86_RS20380 and ABZR86_RS20385 are separated by 169 nucleotides
ABZR86_RS20365: ABZR86_RS20365 - response regulator transcription factor, at 10,576 to 11,238
_RS20365
ABZR86_RS20370: ABZR86_RS20370 - alpha/beta hydrolase, at 11,327 to 12,127
_RS20370
ABZR86_RS20375: ABZR86_RS20375 - nitrilase-related carbon-nitrogen hydrolase, at 12,148 to 13,575
_RS20375
ABZR86_RS20380: ABZR86_RS20380 - TetR/AcrR family transcriptional regulator, at 13,559 to 14,140
_RS20380
ABZR86_RS20385: ABZR86_RS20385 - STAS domain-containing protein, at 14,310 to 14,702
_RS20385
Position (kb) 12
13
14 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 11.234 kb on + strand at 11.234 kb on + strand at 11.625 kb on + strand, within ABZR86_RS20370 at 11.626 kb on - strand, within ABZR86_RS20370 at 11.931 kb on + strand, within ABZR86_RS20370 at 12.243 kb on - strand at 12.243 kb on - strand at 12.243 kb on - strand at 12.486 kb on - strand, within ABZR86_RS20375 at 12.564 kb on - strand, within ABZR86_RS20375 at 12.582 kb on - strand, within ABZR86_RS20375 at 12.941 kb on + strand, within ABZR86_RS20375 at 12.953 kb on + strand, within ABZR86_RS20375 at 12.953 kb on + strand, within ABZR86_RS20375 at 13.190 kb on + strand, within ABZR86_RS20375 at 13.191 kb on - strand, within ABZR86_RS20375 at 13.355 kb on + strand, within ABZR86_RS20375 at 13.355 kb on + strand, within ABZR86_RS20375 at 13.602 kb on + strand at 13.878 kb on + strand, within ABZR86_RS20380 at 13.878 kb on + strand, within ABZR86_RS20380 at 14.301 kb on + strand at 14.301 kb on + strand at 14.302 kb on - strand at 14.311 kb on + strand at 14.312 kb on - strand at 14.350 kb on - strand, within ABZR86_RS20385 at 14.363 kb on - strand, within ABZR86_RS20385 at 14.466 kb on - strand, within ABZR86_RS20385 at 14.466 kb on - strand, within ABZR86_RS20385 at 14.533 kb on + strand, within ABZR86_RS20385
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.08 mg/ml remove 11,234 + -1.0 11,234 + +0.5 11,625 + ABZR86_RS20370 0.37 +0.9 11,626 - ABZR86_RS20370 0.37 +1.7 11,931 + ABZR86_RS20370 0.75 +0.8 12,243 - +1.0 12,243 - -0.2 12,243 - +0.7 12,486 - ABZR86_RS20375 0.24 -0.1 12,564 - ABZR86_RS20375 0.29 +0.1 12,582 - ABZR86_RS20375 0.30 -0.4 12,941 + ABZR86_RS20375 0.56 -0.3 12,953 + ABZR86_RS20375 0.56 +0.1 12,953 + ABZR86_RS20375 0.56 +0.1 13,190 + ABZR86_RS20375 0.73 -1.0 13,191 - ABZR86_RS20375 0.73 -0.2 13,355 + ABZR86_RS20375 0.85 +0.2 13,355 + ABZR86_RS20375 0.85 -0.5 13,602 + -0.4 13,878 + ABZR86_RS20380 0.55 -0.3 13,878 + ABZR86_RS20380 0.55 -0.0 14,301 + -2.0 14,301 + -3.1 14,302 - -1.0 14,311 + +0.7 14,312 - -0.2 14,350 - ABZR86_RS20385 0.10 +0.5 14,363 - ABZR86_RS20385 0.13 -0.4 14,466 - ABZR86_RS20385 0.40 +1.0 14,466 - ABZR86_RS20385 0.40 +0.6 14,533 + ABZR86_RS20385 0.57 +1.3
Or see this region's nucleotide sequence