Experiment: Nalidixic 0.08 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS19245 and ABZR86_RS19250 overlap by 4 nucleotides ABZR86_RS19250 and ABZR86_RS19255 are separated by 83 nucleotides ABZR86_RS19255 and ABZR86_RS19260 are separated by 473 nucleotides
ABZR86_RS19245: ABZR86_RS19245 - glycosyltransferase family 2 protein, at 306,211 to 307,107
_RS19245
ABZR86_RS19250: ABZR86_RS19250 - DMT family transporter, at 307,104 to 308,006
_RS19250
ABZR86_RS19255: ABZR86_RS19255 - DUF2127 domain-containing protein, at 308,090 to 308,599
_RS19255
ABZR86_RS19260: ABZR86_RS19260 - filamentous hemagglutinin N-terminal domain-containing protein, at 309,073 to 311,274
_RS19260
Position (kb) 308
309 Strain fitness (log2 ratio) -2
-1
0
1
2 at 307.382 kb on + strand, within ABZR86_RS19250 at 307.382 kb on + strand, within ABZR86_RS19250 at 307.383 kb on - strand, within ABZR86_RS19250 at 307.383 kb on - strand, within ABZR86_RS19250 at 307.383 kb on - strand, within ABZR86_RS19250 at 307.383 kb on - strand, within ABZR86_RS19250 at 307.419 kb on + strand, within ABZR86_RS19250 at 307.419 kb on + strand, within ABZR86_RS19250 at 307.419 kb on + strand, within ABZR86_RS19250 at 307.419 kb on + strand, within ABZR86_RS19250 at 307.419 kb on + strand, within ABZR86_RS19250 at 307.419 kb on + strand, within ABZR86_RS19250 at 307.419 kb on + strand, within ABZR86_RS19250 at 307.419 kb on + strand, within ABZR86_RS19250 at 307.419 kb on + strand, within ABZR86_RS19250 at 307.419 kb on + strand, within ABZR86_RS19250 at 307.419 kb on + strand, within ABZR86_RS19250 at 307.419 kb on + strand, within ABZR86_RS19250 at 307.420 kb on - strand, within ABZR86_RS19250 at 307.420 kb on - strand, within ABZR86_RS19250 at 307.420 kb on - strand, within ABZR86_RS19250 at 307.420 kb on - strand, within ABZR86_RS19250 at 307.585 kb on - strand, within ABZR86_RS19250 at 307.585 kb on - strand, within ABZR86_RS19250 at 307.746 kb on + strand, within ABZR86_RS19250 at 307.746 kb on + strand, within ABZR86_RS19250 at 307.746 kb on + strand, within ABZR86_RS19250 at 307.746 kb on + strand, within ABZR86_RS19250 at 307.747 kb on - strand, within ABZR86_RS19250 at 307.747 kb on - strand, within ABZR86_RS19250 at 307.747 kb on - strand, within ABZR86_RS19250 at 307.806 kb on - strand, within ABZR86_RS19250 at 307.938 kb on + strand at 307.938 kb on + strand at 307.938 kb on + strand at 307.939 kb on - strand at 307.939 kb on - strand at 308.004 kb on + strand at 308.005 kb on - strand at 308.091 kb on + strand at 308.091 kb on + strand at 308.091 kb on + strand at 308.152 kb on + strand, within ABZR86_RS19255 at 308.206 kb on - strand, within ABZR86_RS19255 at 308.277 kb on - strand, within ABZR86_RS19255 at 308.277 kb on - strand, within ABZR86_RS19255 at 308.311 kb on - strand, within ABZR86_RS19255 at 308.514 kb on + strand, within ABZR86_RS19255 at 308.514 kb on + strand, within ABZR86_RS19255 at 308.515 kb on - strand, within ABZR86_RS19255 at 308.515 kb on - strand, within ABZR86_RS19255 at 308.612 kb on - strand at 308.788 kb on + strand at 308.788 kb on + strand at 308.789 kb on - strand at 309.051 kb on + strand at 309.052 kb on - strand at 309.066 kb on + strand at 309.066 kb on + strand at 309.066 kb on + strand at 309.067 kb on - strand at 309.094 kb on + strand at 309.095 kb on - strand at 309.180 kb on + strand at 309.180 kb on + strand at 309.181 kb on - strand at 309.195 kb on + strand at 309.196 kb on - strand at 309.221 kb on + strand at 309.221 kb on + strand at 309.221 kb on + strand at 309.222 kb on - strand at 309.280 kb on - strand at 309.280 kb on - strand at 309.280 kb on - strand at 309.280 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.08 mg/ml remove 307,382 + ABZR86_RS19250 0.31 +0.2 307,382 + ABZR86_RS19250 0.31 +0.3 307,383 - ABZR86_RS19250 0.31 -1.9 307,383 - ABZR86_RS19250 0.31 -0.1 307,383 - ABZR86_RS19250 0.31 -0.5 307,383 - ABZR86_RS19250 0.31 -0.4 307,419 + ABZR86_RS19250 0.35 +1.0 307,419 + ABZR86_RS19250 0.35 +1.4 307,419 + ABZR86_RS19250 0.35 -1.4 307,419 + ABZR86_RS19250 0.35 -0.7 307,419 + ABZR86_RS19250 0.35 -0.3 307,419 + ABZR86_RS19250 0.35 +0.9 307,419 + ABZR86_RS19250 0.35 -2.0 307,419 + ABZR86_RS19250 0.35 -0.6 307,419 + ABZR86_RS19250 0.35 +0.3 307,419 + ABZR86_RS19250 0.35 +0.2 307,419 + ABZR86_RS19250 0.35 -0.9 307,419 + ABZR86_RS19250 0.35 +0.1 307,420 - ABZR86_RS19250 0.35 -0.3 307,420 - ABZR86_RS19250 0.35 -0.1 307,420 - ABZR86_RS19250 0.35 +0.1 307,420 - ABZR86_RS19250 0.35 -1.2 307,585 - ABZR86_RS19250 0.53 -0.2 307,585 - ABZR86_RS19250 0.53 -0.1 307,746 + ABZR86_RS19250 0.71 +2.5 307,746 + ABZR86_RS19250 0.71 -0.1 307,746 + ABZR86_RS19250 0.71 -0.1 307,746 + ABZR86_RS19250 0.71 -0.1 307,747 - ABZR86_RS19250 0.71 -0.2 307,747 - ABZR86_RS19250 0.71 -1.5 307,747 - ABZR86_RS19250 0.71 +0.3 307,806 - ABZR86_RS19250 0.78 +0.2 307,938 + -1.1 307,938 + +0.2 307,938 + +1.0 307,939 - -2.1 307,939 - -1.1 308,004 + +0.4 308,005 - +0.4 308,091 + +0.9 308,091 + +0.5 308,091 + +0.5 308,152 + ABZR86_RS19255 0.12 -0.5 308,206 - ABZR86_RS19255 0.23 +0.5 308,277 - ABZR86_RS19255 0.37 -0.7 308,277 - ABZR86_RS19255 0.37 +0.2 308,311 - ABZR86_RS19255 0.43 -0.1 308,514 + ABZR86_RS19255 0.83 +0.5 308,514 + ABZR86_RS19255 0.83 -0.5 308,515 - ABZR86_RS19255 0.83 -0.3 308,515 - ABZR86_RS19255 0.83 -0.4 308,612 - -0.7 308,788 + +0.1 308,788 + -1.0 308,789 - +0.1 309,051 + -0.7 309,052 - +0.0 309,066 + -0.1 309,066 + -1.3 309,066 + +0.2 309,067 - +0.0 309,094 + +0.2 309,095 - +0.3 309,180 + +2.1 309,180 + -0.2 309,181 - -0.4 309,195 + -1.2 309,196 - -0.6 309,221 + -0.0 309,221 + +0.1 309,221 + -0.8 309,222 - -0.2 309,280 - +0.4 309,280 - -0.3 309,280 - -1.7 309,280 - +0.3
Or see this region's nucleotide sequence