Experiment: Nalidixic 0.08 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS18270 and ABZR86_RS18275 are separated by 27 nucleotides ABZR86_RS18275 and ABZR86_RS18280 are separated by 141 nucleotides ABZR86_RS18280 and ABZR86_RS18285 are separated by 14 nucleotides
ABZR86_RS18270: ABZR86_RS18270 - hypothetical protein, at 71,240 to 71,935
_RS18270
ABZR86_RS18275: ABZR86_RS18275 - TetR/AcrR family transcriptional regulator, at 71,963 to 72,577
_RS18275
ABZR86_RS18280: ABZR86_RS18280 - efflux RND transporter periplasmic adaptor subunit, at 72,719 to 73,909
_RS18280
ABZR86_RS18285: ABZR86_RS18285 - efflux RND transporter permease subunit, at 73,924 to 77,067
_RS18285
Position (kb) 72
73
74 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 71.802 kb on + strand, within ABZR86_RS18270 at 71.832 kb on - strand, within ABZR86_RS18270 at 71.832 kb on - strand, within ABZR86_RS18270 at 71.897 kb on + strand at 71.985 kb on + strand at 72.033 kb on + strand, within ABZR86_RS18275 at 72.213 kb on + strand, within ABZR86_RS18275 at 72.213 kb on + strand, within ABZR86_RS18275 at 72.214 kb on - strand, within ABZR86_RS18275 at 72.214 kb on - strand, within ABZR86_RS18275 at 72.444 kb on + strand, within ABZR86_RS18275 at 72.444 kb on + strand, within ABZR86_RS18275 at 72.445 kb on - strand, within ABZR86_RS18275 at 72.612 kb on - strand at 72.615 kb on + strand at 72.615 kb on + strand at 72.616 kb on - strand at 72.668 kb on + strand at 72.686 kb on - strand at 72.902 kb on + strand, within ABZR86_RS18280 at 72.902 kb on + strand, within ABZR86_RS18280 at 72.902 kb on + strand, within ABZR86_RS18280 at 72.902 kb on + strand, within ABZR86_RS18280 at 72.903 kb on - strand, within ABZR86_RS18280 at 72.903 kb on - strand, within ABZR86_RS18280 at 72.996 kb on - strand, within ABZR86_RS18280 at 73.016 kb on + strand, within ABZR86_RS18280 at 73.016 kb on + strand, within ABZR86_RS18280 at 73.016 kb on + strand, within ABZR86_RS18280 at 73.016 kb on + strand, within ABZR86_RS18280 at 73.016 kb on + strand, within ABZR86_RS18280 at 73.017 kb on - strand, within ABZR86_RS18280 at 73.028 kb on + strand, within ABZR86_RS18280 at 73.029 kb on - strand, within ABZR86_RS18280 at 73.101 kb on - strand, within ABZR86_RS18280 at 73.352 kb on + strand, within ABZR86_RS18280 at 73.352 kb on + strand, within ABZR86_RS18280 at 73.352 kb on + strand, within ABZR86_RS18280 at 73.353 kb on - strand, within ABZR86_RS18280 at 73.478 kb on + strand, within ABZR86_RS18280 at 73.592 kb on + strand, within ABZR86_RS18280 at 73.592 kb on + strand, within ABZR86_RS18280 at 73.592 kb on + strand, within ABZR86_RS18280 at 73.592 kb on + strand, within ABZR86_RS18280 at 73.593 kb on - strand, within ABZR86_RS18280 at 73.593 kb on - strand, within ABZR86_RS18280 at 73.593 kb on - strand, within ABZR86_RS18280 at 73.593 kb on - strand, within ABZR86_RS18280 at 73.907 kb on + strand at 73.907 kb on + strand at 73.908 kb on - strand at 73.908 kb on - strand at 74.152 kb on + strand at 74.152 kb on + strand at 74.152 kb on + strand at 74.152 kb on + strand at 74.153 kb on - strand at 74.467 kb on + strand, within ABZR86_RS18285 at 74.531 kb on + strand, within ABZR86_RS18285 at 74.532 kb on - strand, within ABZR86_RS18285 at 74.532 kb on - strand, within ABZR86_RS18285 at 74.532 kb on - strand, within ABZR86_RS18285 at 74.532 kb on - strand, within ABZR86_RS18285 at 74.532 kb on - strand, within ABZR86_RS18285 at 74.532 kb on - strand, within ABZR86_RS18285 at 74.532 kb on - strand, within ABZR86_RS18285 at 74.827 kb on - strand, within ABZR86_RS18285
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.08 mg/ml remove 71,802 + ABZR86_RS18270 0.81 +0.3 71,832 - ABZR86_RS18270 0.85 +0.5 71,832 - ABZR86_RS18270 0.85 +0.6 71,897 + -0.7 71,985 + +0.4 72,033 + ABZR86_RS18275 0.11 -0.1 72,213 + ABZR86_RS18275 0.41 +0.2 72,213 + ABZR86_RS18275 0.41 -1.5 72,214 - ABZR86_RS18275 0.41 -0.2 72,214 - ABZR86_RS18275 0.41 +0.1 72,444 + ABZR86_RS18275 0.78 +0.1 72,444 + ABZR86_RS18275 0.78 +0.4 72,445 - ABZR86_RS18275 0.78 +0.1 72,612 - +0.3 72,615 + +0.1 72,615 + -0.3 72,616 - +0.4 72,668 + -0.0 72,686 - -0.1 72,902 + ABZR86_RS18280 0.15 +0.5 72,902 + ABZR86_RS18280 0.15 -0.2 72,902 + ABZR86_RS18280 0.15 +0.2 72,902 + ABZR86_RS18280 0.15 +0.5 72,903 - ABZR86_RS18280 0.15 +0.0 72,903 - ABZR86_RS18280 0.15 -0.5 72,996 - ABZR86_RS18280 0.23 -0.5 73,016 + ABZR86_RS18280 0.25 +0.6 73,016 + ABZR86_RS18280 0.25 -0.4 73,016 + ABZR86_RS18280 0.25 +0.1 73,016 + ABZR86_RS18280 0.25 -3.1 73,016 + ABZR86_RS18280 0.25 +0.5 73,017 - ABZR86_RS18280 0.25 +0.9 73,028 + ABZR86_RS18280 0.26 +0.2 73,029 - ABZR86_RS18280 0.26 -1.6 73,101 - ABZR86_RS18280 0.32 +0.8 73,352 + ABZR86_RS18280 0.53 -0.5 73,352 + ABZR86_RS18280 0.53 +0.1 73,352 + ABZR86_RS18280 0.53 -0.2 73,353 - ABZR86_RS18280 0.53 -1.5 73,478 + ABZR86_RS18280 0.64 +0.2 73,592 + ABZR86_RS18280 0.73 +0.0 73,592 + ABZR86_RS18280 0.73 -0.2 73,592 + ABZR86_RS18280 0.73 -0.3 73,592 + ABZR86_RS18280 0.73 -0.5 73,593 - ABZR86_RS18280 0.73 +0.4 73,593 - ABZR86_RS18280 0.73 -0.3 73,593 - ABZR86_RS18280 0.73 +0.8 73,593 - ABZR86_RS18280 0.73 +0.4 73,907 + +0.8 73,907 + +0.3 73,908 - -0.1 73,908 - -0.5 74,152 + -1.0 74,152 + -0.8 74,152 + +0.7 74,152 + +0.2 74,153 - -0.1 74,467 + ABZR86_RS18285 0.17 -0.1 74,531 + ABZR86_RS18285 0.19 +0.5 74,532 - ABZR86_RS18285 0.19 -1.6 74,532 - ABZR86_RS18285 0.19 -0.5 74,532 - ABZR86_RS18285 0.19 -0.0 74,532 - ABZR86_RS18285 0.19 +0.3 74,532 - ABZR86_RS18285 0.19 +0.1 74,532 - ABZR86_RS18285 0.19 -0.1 74,532 - ABZR86_RS18285 0.19 -0.0 74,827 - ABZR86_RS18285 0.29 +0.0
Or see this region's nucleotide sequence